Micro-Environmental Variation in Soil Microbial Biodiversity in Forest Frontier Ecosystems—Implications for Sustainability Assessments

Autores
Alvarez, Valeria Esther; El Mujtar, Veronica Andrea; Salles, Joana Falcão; Xiu, Jia; Cardozo, Andrea; Tittonell, Pablo Adrian
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Soil health indicators based on microbial biodiversity are increasingly used in agricultural sustainability assessments. However, little is known about how microbial communities vary within micro-environmental gradients across different land uses, which is crucial for designing field sampling and monitoring protocols. Our objective was to assess how soil microbial communities changed with soil depth and spatial distance across land-use types. We sampled soils in four spatial distances (within 0.1–70 m) and four depths (within 0–40 cm) in forests, grasslands, and horticultural lands, and combined 16S rRNA gene sequencing, DNA quantification and soil chemical characterization to explore micro-environmental variation in microbial biomass, α-β-diversity, and communities’ assembly processes. Depth and spatial distance had differential effects on microbial biodiversity within different land uses. Microbial biomass was most sensitive to depth, α-diversity to spatial distance, and β-diversity to both depth and spatial distance. Deterministic processes dominate microbial communities’ assembly along depth in all land uses, which is a promising result for developing soil quality indicators based on microbial biodiversity. Overall, our results suggest that collecting soil samples separated by at least 12 m is adequate to capture biodiversity changes across land uses. However, collecting randomly within the first 10 cm is recommended for native forests, while systematic sampling within the first 20 cm is advised for grasslands and horticultural lands. Our findings underscore the need for land use-specific sampling frameworks in soil life-based sustainability assessments for meaningful regional comparisons.
EEA Bariloche
Fil: Alvarez, Valeria Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche (IFAB); Argentina
Fil: Alvarez, Valeria Esther. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche (IFAB); Argentina
Fil: Alvarez, Valeria Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Tecnológico de Chascomús; Argentina
Fil: Alvarez, Valeria Esther. Universidad Nacional de San Martín. Instituto Tecnológico de Chascomús; Argentina
Fil: El Mujtar, Veronica Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche (IFAB); Argentina
Fil: El Mujtar, Veronica Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche (IFAB); Argentina
Fil: Salles, Joana Falcão. Groningen University. Groningen Institute of Evolutionary Life Sciences (GELIFES); Países Bajos
Fil: Xiu, Jia. Groningen University. Groningen Institute of Evolutionary Life Sciences (GELIFES); Países Bajos
Fil: Castan, Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche (IFAB); Argentina
Fil: Castan, Elisa. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche (IFAB); Argentina
Fil: Cardozo, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche (IFAB); Argentina
Fil: Cardozo, Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche (IFAB); Argentina
Fil: Cardozo, Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Agencia de Extensión Rural El Bolsón; Argentina
Fil: Tittonell, Pablo Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche (IFAB); Argentina
Fil: Tittonell, Pablo Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche (IFAB); Argentina
Fil: Tittonell, Pablo Adrian. Groningen University. Groningen Institute of Evolutionary Life Sciences; Países Bajos
Fil: Tittonell, Pablo Adrian. Université de Montpellier. Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD). Agroécologie et Intensification Durable (AïDA); Francia
Fuente
Sustainability 16 (3) : 1236. (February 2024)
Materia
Ecosistema
Ecosistemas Forestales
Microorganismos del Suelo
Biodiversidad
Sostenibilidad
Medio Ambiente
Ecosystems
Forest Ecosystems
Soil Microorganisms
Biodiversity
Sustainability
Environment
Región Patagónica
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/16799
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spelling Micro-Environmental Variation in Soil Microbial Biodiversity in Forest Frontier Ecosystems—Implications for Sustainability AssessmentsAlvarez, Valeria EstherEl Mujtar, Veronica AndreaSalles, Joana FalcãoXiu, JiaCardozo, AndreaTittonell, Pablo AdrianEcosistemaEcosistemas ForestalesMicroorganismos del SueloBiodiversidadSostenibilidadMedio AmbienteEcosystemsForest EcosystemsSoil MicroorganismsBiodiversitySustainabilityEnvironmentRegión PatagónicaSoil health indicators based on microbial biodiversity are increasingly used in agricultural sustainability assessments. However, little is known about how microbial communities vary within micro-environmental gradients across different land uses, which is crucial for designing field sampling and monitoring protocols. Our objective was to assess how soil microbial communities changed with soil depth and spatial distance across land-use types. We sampled soils in four spatial distances (within 0.1–70 m) and four depths (within 0–40 cm) in forests, grasslands, and horticultural lands, and combined 16S rRNA gene sequencing, DNA quantification and soil chemical characterization to explore micro-environmental variation in microbial biomass, α-β-diversity, and communities’ assembly processes. Depth and spatial distance had differential effects on microbial biodiversity within different land uses. Microbial biomass was most sensitive to depth, α-diversity to spatial distance, and β-diversity to both depth and spatial distance. Deterministic processes dominate microbial communities’ assembly along depth in all land uses, which is a promising result for developing soil quality indicators based on microbial biodiversity. Overall, our results suggest that collecting soil samples separated by at least 12 m is adequate to capture biodiversity changes across land uses. However, collecting randomly within the first 10 cm is recommended for native forests, while systematic sampling within the first 20 cm is advised for grasslands and horticultural lands. Our findings underscore the need for land use-specific sampling frameworks in soil life-based sustainability assessments for meaningful regional comparisons.EEA BarilocheFil: Alvarez, Valeria Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche (IFAB); ArgentinaFil: Alvarez, Valeria Esther. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche (IFAB); ArgentinaFil: Alvarez, Valeria Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Tecnológico de Chascomús; ArgentinaFil: Alvarez, Valeria Esther. Universidad Nacional de San Martín. Instituto Tecnológico de Chascomús; ArgentinaFil: El Mujtar, Veronica Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche (IFAB); ArgentinaFil: El Mujtar, Veronica Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche (IFAB); ArgentinaFil: Salles, Joana Falcão. Groningen University. Groningen Institute of Evolutionary Life Sciences (GELIFES); Países BajosFil: Xiu, Jia. Groningen University. Groningen Institute of Evolutionary Life Sciences (GELIFES); Países BajosFil: Castan, Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche (IFAB); ArgentinaFil: Castan, Elisa. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche (IFAB); ArgentinaFil: Cardozo, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche (IFAB); ArgentinaFil: Cardozo, Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche (IFAB); ArgentinaFil: Cardozo, Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Agencia de Extensión Rural El Bolsón; ArgentinaFil: Tittonell, Pablo Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche (IFAB); ArgentinaFil: Tittonell, Pablo Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche (IFAB); ArgentinaFil: Tittonell, Pablo Adrian. Groningen University. Groningen Institute of Evolutionary Life Sciences; Países BajosFil: Tittonell, Pablo Adrian. Université de Montpellier. Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD). Agroécologie et Intensification Durable (AïDA); FranciaMDPI2024-02-26T13:42:39Z2024-02-26T13:42:39Z2024-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/16799https://www.mdpi.com/2071-1050/16/3/12362071-1050https://doi.org/10.3390/su16031236Sustainability 16 (3) : 1236. (February 2024)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PE-E1-I020-001, Desarrollo de criterios para diseño, monitoreo y evaluación de estrategias de intensificación sostenible de agroecosistemas, basadas en múltiples servicios ecosistémicosinfo:eu-repograntAgreement/INTA/2019-PD-E2-I037-002, Biodiversidad edáfica: componente clave para una gestión integral y sustentable del recurso sueloinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-23T11:18:42Zoai:localhost:20.500.12123/16799instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:18:43.27INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Micro-Environmental Variation in Soil Microbial Biodiversity in Forest Frontier Ecosystems—Implications for Sustainability Assessments
title Micro-Environmental Variation in Soil Microbial Biodiversity in Forest Frontier Ecosystems—Implications for Sustainability Assessments
spellingShingle Micro-Environmental Variation in Soil Microbial Biodiversity in Forest Frontier Ecosystems—Implications for Sustainability Assessments
Alvarez, Valeria Esther
Ecosistema
Ecosistemas Forestales
Microorganismos del Suelo
Biodiversidad
Sostenibilidad
Medio Ambiente
Ecosystems
Forest Ecosystems
Soil Microorganisms
Biodiversity
Sustainability
Environment
Región Patagónica
title_short Micro-Environmental Variation in Soil Microbial Biodiversity in Forest Frontier Ecosystems—Implications for Sustainability Assessments
title_full Micro-Environmental Variation in Soil Microbial Biodiversity in Forest Frontier Ecosystems—Implications for Sustainability Assessments
title_fullStr Micro-Environmental Variation in Soil Microbial Biodiversity in Forest Frontier Ecosystems—Implications for Sustainability Assessments
title_full_unstemmed Micro-Environmental Variation in Soil Microbial Biodiversity in Forest Frontier Ecosystems—Implications for Sustainability Assessments
title_sort Micro-Environmental Variation in Soil Microbial Biodiversity in Forest Frontier Ecosystems—Implications for Sustainability Assessments
dc.creator.none.fl_str_mv Alvarez, Valeria Esther
El Mujtar, Veronica Andrea
Salles, Joana Falcão
Xiu, Jia
Cardozo, Andrea
Tittonell, Pablo Adrian
author Alvarez, Valeria Esther
author_facet Alvarez, Valeria Esther
El Mujtar, Veronica Andrea
Salles, Joana Falcão
Xiu, Jia
Cardozo, Andrea
Tittonell, Pablo Adrian
author_role author
author2 El Mujtar, Veronica Andrea
Salles, Joana Falcão
Xiu, Jia
Cardozo, Andrea
Tittonell, Pablo Adrian
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ecosistema
Ecosistemas Forestales
Microorganismos del Suelo
Biodiversidad
Sostenibilidad
Medio Ambiente
Ecosystems
Forest Ecosystems
Soil Microorganisms
Biodiversity
Sustainability
Environment
Región Patagónica
topic Ecosistema
Ecosistemas Forestales
Microorganismos del Suelo
Biodiversidad
Sostenibilidad
Medio Ambiente
Ecosystems
Forest Ecosystems
Soil Microorganisms
Biodiversity
Sustainability
Environment
Región Patagónica
dc.description.none.fl_txt_mv Soil health indicators based on microbial biodiversity are increasingly used in agricultural sustainability assessments. However, little is known about how microbial communities vary within micro-environmental gradients across different land uses, which is crucial for designing field sampling and monitoring protocols. Our objective was to assess how soil microbial communities changed with soil depth and spatial distance across land-use types. We sampled soils in four spatial distances (within 0.1–70 m) and four depths (within 0–40 cm) in forests, grasslands, and horticultural lands, and combined 16S rRNA gene sequencing, DNA quantification and soil chemical characterization to explore micro-environmental variation in microbial biomass, α-β-diversity, and communities’ assembly processes. Depth and spatial distance had differential effects on microbial biodiversity within different land uses. Microbial biomass was most sensitive to depth, α-diversity to spatial distance, and β-diversity to both depth and spatial distance. Deterministic processes dominate microbial communities’ assembly along depth in all land uses, which is a promising result for developing soil quality indicators based on microbial biodiversity. Overall, our results suggest that collecting soil samples separated by at least 12 m is adequate to capture biodiversity changes across land uses. However, collecting randomly within the first 10 cm is recommended for native forests, while systematic sampling within the first 20 cm is advised for grasslands and horticultural lands. Our findings underscore the need for land use-specific sampling frameworks in soil life-based sustainability assessments for meaningful regional comparisons.
EEA Bariloche
Fil: Alvarez, Valeria Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche (IFAB); Argentina
Fil: Alvarez, Valeria Esther. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche (IFAB); Argentina
Fil: Alvarez, Valeria Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Tecnológico de Chascomús; Argentina
Fil: Alvarez, Valeria Esther. Universidad Nacional de San Martín. Instituto Tecnológico de Chascomús; Argentina
Fil: El Mujtar, Veronica Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche (IFAB); Argentina
Fil: El Mujtar, Veronica Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche (IFAB); Argentina
Fil: Salles, Joana Falcão. Groningen University. Groningen Institute of Evolutionary Life Sciences (GELIFES); Países Bajos
Fil: Xiu, Jia. Groningen University. Groningen Institute of Evolutionary Life Sciences (GELIFES); Países Bajos
Fil: Castan, Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche (IFAB); Argentina
Fil: Castan, Elisa. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche (IFAB); Argentina
Fil: Cardozo, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche (IFAB); Argentina
Fil: Cardozo, Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche (IFAB); Argentina
Fil: Cardozo, Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Agencia de Extensión Rural El Bolsón; Argentina
Fil: Tittonell, Pablo Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche (IFAB); Argentina
Fil: Tittonell, Pablo Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche (IFAB); Argentina
Fil: Tittonell, Pablo Adrian. Groningen University. Groningen Institute of Evolutionary Life Sciences; Países Bajos
Fil: Tittonell, Pablo Adrian. Université de Montpellier. Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD). Agroécologie et Intensification Durable (AïDA); Francia
description Soil health indicators based on microbial biodiversity are increasingly used in agricultural sustainability assessments. However, little is known about how microbial communities vary within micro-environmental gradients across different land uses, which is crucial for designing field sampling and monitoring protocols. Our objective was to assess how soil microbial communities changed with soil depth and spatial distance across land-use types. We sampled soils in four spatial distances (within 0.1–70 m) and four depths (within 0–40 cm) in forests, grasslands, and horticultural lands, and combined 16S rRNA gene sequencing, DNA quantification and soil chemical characterization to explore micro-environmental variation in microbial biomass, α-β-diversity, and communities’ assembly processes. Depth and spatial distance had differential effects on microbial biodiversity within different land uses. Microbial biomass was most sensitive to depth, α-diversity to spatial distance, and β-diversity to both depth and spatial distance. Deterministic processes dominate microbial communities’ assembly along depth in all land uses, which is a promising result for developing soil quality indicators based on microbial biodiversity. Overall, our results suggest that collecting soil samples separated by at least 12 m is adequate to capture biodiversity changes across land uses. However, collecting randomly within the first 10 cm is recommended for native forests, while systematic sampling within the first 20 cm is advised for grasslands and horticultural lands. Our findings underscore the need for land use-specific sampling frameworks in soil life-based sustainability assessments for meaningful regional comparisons.
publishDate 2024
dc.date.none.fl_str_mv 2024-02-26T13:42:39Z
2024-02-26T13:42:39Z
2024-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/16799
https://www.mdpi.com/2071-1050/16/3/1236
2071-1050
https://doi.org/10.3390/su16031236
url http://hdl.handle.net/20.500.12123/16799
https://www.mdpi.com/2071-1050/16/3/1236
https://doi.org/10.3390/su16031236
identifier_str_mv 2071-1050
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PE-E1-I020-001, Desarrollo de criterios para diseño, monitoreo y evaluación de estrategias de intensificación sostenible de agroecosistemas, basadas en múltiples servicios ecosistémicos
info:eu-repograntAgreement/INTA/2019-PD-E2-I037-002, Biodiversidad edáfica: componente clave para una gestión integral y sustentable del recurso suelo
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Sustainability 16 (3) : 1236. (February 2024)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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