Trends of two epidemic multidrug-resistant strains of Mycobacterium tuberculosis in Argentina disclosed by tailored molecular strategy

Autores
Monteserin, Johana; Pérez Lago, Laura; Yokobori, Noemí; Paul, Roxana Elizabeth; Rodríguez Maus, Sandra; Símboli, Norberto Fabián; Eldholm, Vegard; Lopez, Beatriz; García de Viedma, Darío; Ritacco, Gloria Viviana
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Two Mycobacterium tuberculosis strains-M (sublineage 4.1) and Ra (sublineage 4.3)-have long prevailed in Argentina among patients with multidrug-resistant tuberculosis (MDR-TB). Recently, budget constraints have hampered the surveillance of MDR-TB transmission. Based on whole-genome sequence analysis, we used M- and Ra-specific single nucleotide polymorphisms to tailor two multiplex allele-specific polymerase chain reactions (PCRs), which we applied to 252 stored isolates (95% of all newly diagnosed MDR-TB cases countrywide, 2015-2017). Compared with the latest data available (2007-2009), the M strain has receded (80/324 to 20/252, P < 0.0001), particularly among cross-border migrants (12/58 to 0/53, P = 0.0003) and HIV-infected people (30/97 to 7/74, P = 0.0007), but it still accounts for 4/12 new cases of extensively drug-resistant TB. Differently, the Ra strain remained stable in frequency (39/324 to 33/252) and contributed marginally to the extensive drug-resistance load (1/12). Our novel strategy disclosed recent trends of the two major MDR-TB strains, providing meaningful data to allocate control interventions more efficiently.
Fil: Monteserin, Johana. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pérez Lago, Laura. Instituto de Investigación Sanitaria Gregorio Marañón; España
Fil: Yokobori, Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Paul, Roxana Elizabeth. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Rodríguez Maus, Sandra. Instituto de Investigación Sanitaria Gregorio Marañón; España
Fil: Símboli, Norberto Fabián. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Eldholm, Vegard. Norwegian Institute Of Public Health; Noruega
Fil: Lopez, Beatriz. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: García de Viedma, Darío. Hospital General Universitario Gregorio Marañón; España
Fil: Ritacco, Gloria Viviana. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Multidrug resistant tuberculosis
genotyping
Whole genome sequence
Single Nucleotid Polymorphism
PCR
Surveillance
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/175265
Acceder
id CONICETDig_36627ddf39de358204929ab270f68f66
oai_identifier_str oai:ri.conicet.gov.ar:11336/175265
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Trends of two epidemic multidrug-resistant strains of Mycobacterium tuberculosis in Argentina disclosed by tailored molecular strategyMonteserin, JohanaPérez Lago, LauraYokobori, NoemíPaul, Roxana ElizabethRodríguez Maus, SandraSímboli, Norberto FabiánEldholm, VegardLopez, BeatrizGarcía de Viedma, DaríoRitacco, Gloria VivianaMultidrug resistant tuberculosisgenotypingWhole genome sequenceSingle Nucleotid PolymorphismPCRSurveillancehttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Two Mycobacterium tuberculosis strains-M (sublineage 4.1) and Ra (sublineage 4.3)-have long prevailed in Argentina among patients with multidrug-resistant tuberculosis (MDR-TB). Recently, budget constraints have hampered the surveillance of MDR-TB transmission. Based on whole-genome sequence analysis, we used M- and Ra-specific single nucleotide polymorphisms to tailor two multiplex allele-specific polymerase chain reactions (PCRs), which we applied to 252 stored isolates (95% of all newly diagnosed MDR-TB cases countrywide, 2015-2017). Compared with the latest data available (2007-2009), the M strain has receded (80/324 to 20/252, P < 0.0001), particularly among cross-border migrants (12/58 to 0/53, P = 0.0003) and HIV-infected people (30/97 to 7/74, P = 0.0007), but it still accounts for 4/12 new cases of extensively drug-resistant TB. Differently, the Ra strain remained stable in frequency (39/324 to 33/252) and contributed marginally to the extensive drug-resistance load (1/12). Our novel strategy disclosed recent trends of the two major MDR-TB strains, providing meaningful data to allocate control interventions more efficiently.Fil: Monteserin, Johana. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pérez Lago, Laura. Instituto de Investigación Sanitaria Gregorio Marañón; EspañaFil: Yokobori, Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; ArgentinaFil: Paul, Roxana Elizabeth. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; ArgentinaFil: Rodríguez Maus, Sandra. Instituto de Investigación Sanitaria Gregorio Marañón; EspañaFil: Símboli, Norberto Fabián. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; ArgentinaFil: Eldholm, Vegard. Norwegian Institute Of Public Health; NoruegaFil: Lopez, Beatriz. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; ArgentinaFil: García de Viedma, Darío. Hospital General Universitario Gregorio Marañón; EspañaFil: Ritacco, Gloria Viviana. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaAmerican Society of Tropical Medicine and Hygiene2019-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/175265Monteserin, Johana; Pérez Lago, Laura; Yokobori, Noemí; Paul, Roxana Elizabeth; Rodríguez Maus, Sandra; et al.; Trends of two epidemic multidrug-resistant strains of Mycobacterium tuberculosis in Argentina disclosed by tailored molecular strategy; American Society of Tropical Medicine and Hygiene; American Journal of Tropical Medicine and Hygiene; 101; 6; 10-2019; 1308-13110002-9637CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.ajtmh.org/content/journals/10.4269/ajtmh.19-0397info:eu-repo/semantics/altIdentifier/doi/10.4269/ajtmh.19-0397info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:14:17Zoai:ri.conicet.gov.ar:11336/175265instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:14:17.562CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Trends of two epidemic multidrug-resistant strains of Mycobacterium tuberculosis in Argentina disclosed by tailored molecular strategy
title Trends of two epidemic multidrug-resistant strains of Mycobacterium tuberculosis in Argentina disclosed by tailored molecular strategy
spellingShingle Trends of two epidemic multidrug-resistant strains of Mycobacterium tuberculosis in Argentina disclosed by tailored molecular strategy
Monteserin, Johana
Multidrug resistant tuberculosis
genotyping
Whole genome sequence
Single Nucleotid Polymorphism
PCR
Surveillance
title_short Trends of two epidemic multidrug-resistant strains of Mycobacterium tuberculosis in Argentina disclosed by tailored molecular strategy
title_full Trends of two epidemic multidrug-resistant strains of Mycobacterium tuberculosis in Argentina disclosed by tailored molecular strategy
title_fullStr Trends of two epidemic multidrug-resistant strains of Mycobacterium tuberculosis in Argentina disclosed by tailored molecular strategy
title_full_unstemmed Trends of two epidemic multidrug-resistant strains of Mycobacterium tuberculosis in Argentina disclosed by tailored molecular strategy
title_sort Trends of two epidemic multidrug-resistant strains of Mycobacterium tuberculosis in Argentina disclosed by tailored molecular strategy
dc.creator.none.fl_str_mv Monteserin, Johana
Pérez Lago, Laura
Yokobori, Noemí
Paul, Roxana Elizabeth
Rodríguez Maus, Sandra
Símboli, Norberto Fabián
Eldholm, Vegard
Lopez, Beatriz
García de Viedma, Darío
Ritacco, Gloria Viviana
author Monteserin, Johana
author_facet Monteserin, Johana
Pérez Lago, Laura
Yokobori, Noemí
Paul, Roxana Elizabeth
Rodríguez Maus, Sandra
Símboli, Norberto Fabián
Eldholm, Vegard
Lopez, Beatriz
García de Viedma, Darío
Ritacco, Gloria Viviana
author_role author
author2 Pérez Lago, Laura
Yokobori, Noemí
Paul, Roxana Elizabeth
Rodríguez Maus, Sandra
Símboli, Norberto Fabián
Eldholm, Vegard
Lopez, Beatriz
García de Viedma, Darío
Ritacco, Gloria Viviana
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Multidrug resistant tuberculosis
genotyping
Whole genome sequence
Single Nucleotid Polymorphism
PCR
Surveillance
topic Multidrug resistant tuberculosis
genotyping
Whole genome sequence
Single Nucleotid Polymorphism
PCR
Surveillance
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Two Mycobacterium tuberculosis strains-M (sublineage 4.1) and Ra (sublineage 4.3)-have long prevailed in Argentina among patients with multidrug-resistant tuberculosis (MDR-TB). Recently, budget constraints have hampered the surveillance of MDR-TB transmission. Based on whole-genome sequence analysis, we used M- and Ra-specific single nucleotide polymorphisms to tailor two multiplex allele-specific polymerase chain reactions (PCRs), which we applied to 252 stored isolates (95% of all newly diagnosed MDR-TB cases countrywide, 2015-2017). Compared with the latest data available (2007-2009), the M strain has receded (80/324 to 20/252, P < 0.0001), particularly among cross-border migrants (12/58 to 0/53, P = 0.0003) and HIV-infected people (30/97 to 7/74, P = 0.0007), but it still accounts for 4/12 new cases of extensively drug-resistant TB. Differently, the Ra strain remained stable in frequency (39/324 to 33/252) and contributed marginally to the extensive drug-resistance load (1/12). Our novel strategy disclosed recent trends of the two major MDR-TB strains, providing meaningful data to allocate control interventions more efficiently.
Fil: Monteserin, Johana. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pérez Lago, Laura. Instituto de Investigación Sanitaria Gregorio Marañón; España
Fil: Yokobori, Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Paul, Roxana Elizabeth. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Rodríguez Maus, Sandra. Instituto de Investigación Sanitaria Gregorio Marañón; España
Fil: Símboli, Norberto Fabián. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Eldholm, Vegard. Norwegian Institute Of Public Health; Noruega
Fil: Lopez, Beatriz. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: García de Viedma, Darío. Hospital General Universitario Gregorio Marañón; España
Fil: Ritacco, Gloria Viviana. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Two Mycobacterium tuberculosis strains-M (sublineage 4.1) and Ra (sublineage 4.3)-have long prevailed in Argentina among patients with multidrug-resistant tuberculosis (MDR-TB). Recently, budget constraints have hampered the surveillance of MDR-TB transmission. Based on whole-genome sequence analysis, we used M- and Ra-specific single nucleotide polymorphisms to tailor two multiplex allele-specific polymerase chain reactions (PCRs), which we applied to 252 stored isolates (95% of all newly diagnosed MDR-TB cases countrywide, 2015-2017). Compared with the latest data available (2007-2009), the M strain has receded (80/324 to 20/252, P < 0.0001), particularly among cross-border migrants (12/58 to 0/53, P = 0.0003) and HIV-infected people (30/97 to 7/74, P = 0.0007), but it still accounts for 4/12 new cases of extensively drug-resistant TB. Differently, the Ra strain remained stable in frequency (39/324 to 33/252) and contributed marginally to the extensive drug-resistance load (1/12). Our novel strategy disclosed recent trends of the two major MDR-TB strains, providing meaningful data to allocate control interventions more efficiently.
publishDate 2019
dc.date.none.fl_str_mv 2019-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/175265
Monteserin, Johana; Pérez Lago, Laura; Yokobori, Noemí; Paul, Roxana Elizabeth; Rodríguez Maus, Sandra; et al.; Trends of two epidemic multidrug-resistant strains of Mycobacterium tuberculosis in Argentina disclosed by tailored molecular strategy; American Society of Tropical Medicine and Hygiene; American Journal of Tropical Medicine and Hygiene; 101; 6; 10-2019; 1308-1311
0002-9637
CONICET Digital
CONICET
url http://hdl.handle.net/11336/175265
identifier_str_mv Monteserin, Johana; Pérez Lago, Laura; Yokobori, Noemí; Paul, Roxana Elizabeth; Rodríguez Maus, Sandra; et al.; Trends of two epidemic multidrug-resistant strains of Mycobacterium tuberculosis in Argentina disclosed by tailored molecular strategy; American Society of Tropical Medicine and Hygiene; American Journal of Tropical Medicine and Hygiene; 101; 6; 10-2019; 1308-1311
0002-9637
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.ajtmh.org/content/journals/10.4269/ajtmh.19-0397
info:eu-repo/semantics/altIdentifier/doi/10.4269/ajtmh.19-0397
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society of Tropical Medicine and Hygiene
publisher.none.fl_str_mv American Society of Tropical Medicine and Hygiene
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.260194

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