TGFβ-signaling and FOXG1-expression are a hallmark of astrocyte lineage diversity in the murine ventral and dorsal forebrain

Autores
Weise, Stefan Christopher; Villarreal, Alejandro; Heidrich, Stefanie; Dehghanian, Fariba; Schachtrup, Christian; Nestel, Sigrun; Schwarz, Jennifer; Thedieck, Kathrin; Vogel, Tanja
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Heterogeneous astrocyte populations are defined by diversity in cellular environment, progenitor identity or function. Yet, little is known about the extent of the heterogeneity and how this diversity is acquired during development. To investigate the impact of TGF (transforming growth factor) β-signaling on astrocyte development in the telencephalon we deleted the TGFBR2 (transforming growth factor beta receptor 2) in early neural progenitor cells in mice using a FOXG1 (forkhead box G1)-driven CRE-recombinase. We used quantitative proteomics to characterize TGFBR2-deficient cells derived from the mouse telencephalon and identified differential protein expression of the astrocyte proteins GFAP (glial fibrillary acidic protein) and MFGE8 (milk fat globule-EGF factor 8). Biochemical and histological investigations revealed distinct populations of astrocytes in the dorsal and ventral telencephalon marked by GFAP or MFGE8 protein expression. The two subtypes differed in their response to TGFβ-signaling. Impaired TGFβ-signaling affected numbers of GFAP astrocytes in the ventral telencephalon. In contrast, TGFβ reduced MFGE8-expression in astrocytes deriving from both regions. Additionally, lineage tracing revealed that both GFAP and MFGE8 astrocyte subtypes derived partly from FOXG1-expressing neural precursor cells.
Fil: Weise, Stefan Christopher. Universität Freiburg Im Breisgau; Alemania
Fil: Villarreal, Alejandro. Universität Freiburg Im Breisgau; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: Heidrich, Stefanie. Universität Freiburg Im Breisgau; Alemania
Fil: Dehghanian, Fariba. University Of Isfahan; Irán. Universität Freiburg Im Breisgau; Alemania
Fil: Schachtrup, Christian. Universität Freiburg Im Breisgau; Alemania
Fil: Nestel, Sigrun. Universität Freiburg Im Breisgau; Alemania
Fil: Schwarz, Jennifer. Universität Freiburg Im Breisgau; Alemania
Fil: Thedieck, Kathrin. Universität Oldenburg; Argentina. University of Groningen; Países Bajos
Fil: Vogel, Tanja. Universität Freiburg Im Breisgau; Alemania
Materia
LINEAGE-TRACING
NEURAL DIFFERENTIATION
SILAC
TGFBR2-KNOCKOUT
ASTRCYTE-DIVERSITY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/87973
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/87973
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling TGFβ-signaling and FOXG1-expression are a hallmark of astrocyte lineage diversity in the murine ventral and dorsal forebrainWeise, Stefan ChristopherVillarreal, AlejandroHeidrich, StefanieDehghanian, FaribaSchachtrup, ChristianNestel, SigrunSchwarz, JenniferThedieck, KathrinVogel, TanjaLINEAGE-TRACINGNEURAL DIFFERENTIATIONSILACTGFBR2-KNOCKOUTASTRCYTE-DIVERSITYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Heterogeneous astrocyte populations are defined by diversity in cellular environment, progenitor identity or function. Yet, little is known about the extent of the heterogeneity and how this diversity is acquired during development. To investigate the impact of TGF (transforming growth factor) β-signaling on astrocyte development in the telencephalon we deleted the TGFBR2 (transforming growth factor beta receptor 2) in early neural progenitor cells in mice using a FOXG1 (forkhead box G1)-driven CRE-recombinase. We used quantitative proteomics to characterize TGFBR2-deficient cells derived from the mouse telencephalon and identified differential protein expression of the astrocyte proteins GFAP (glial fibrillary acidic protein) and MFGE8 (milk fat globule-EGF factor 8). Biochemical and histological investigations revealed distinct populations of astrocytes in the dorsal and ventral telencephalon marked by GFAP or MFGE8 protein expression. The two subtypes differed in their response to TGFβ-signaling. Impaired TGFβ-signaling affected numbers of GFAP astrocytes in the ventral telencephalon. In contrast, TGFβ reduced MFGE8-expression in astrocytes deriving from both regions. Additionally, lineage tracing revealed that both GFAP and MFGE8 astrocyte subtypes derived partly from FOXG1-expressing neural precursor cells.Fil: Weise, Stefan Christopher. Universität Freiburg Im Breisgau; AlemaniaFil: Villarreal, Alejandro. Universität Freiburg Im Breisgau; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; ArgentinaFil: Heidrich, Stefanie. Universität Freiburg Im Breisgau; AlemaniaFil: Dehghanian, Fariba. University Of Isfahan; Irán. Universität Freiburg Im Breisgau; AlemaniaFil: Schachtrup, Christian. Universität Freiburg Im Breisgau; AlemaniaFil: Nestel, Sigrun. Universität Freiburg Im Breisgau; AlemaniaFil: Schwarz, Jennifer. Universität Freiburg Im Breisgau; AlemaniaFil: Thedieck, Kathrin. Universität Oldenburg; Argentina. University of Groningen; Países BajosFil: Vogel, Tanja. Universität Freiburg Im Breisgau; AlemaniaFrontiers Research Foundation2018-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/87973Weise, Stefan Christopher; Villarreal, Alejandro; Heidrich, Stefanie; Dehghanian, Fariba; Schachtrup, Christian; et al.; TGFβ-signaling and FOXG1-expression are a hallmark of astrocyte lineage diversity in the murine ventral and dorsal forebrain; Frontiers Research Foundation; Frontiers in Cellular Neuroscience; 12; 448; 11-2018; 1-161662-5102CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/article/10.3389/fncel.2018.00448/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fncel.2018.00448info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T10:48:41Zoai:ri.conicet.gov.ar:11336/87973instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 10:48:41.596CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv TGFβ-signaling and FOXG1-expression are a hallmark of astrocyte lineage diversity in the murine ventral and dorsal forebrain
title TGFβ-signaling and FOXG1-expression are a hallmark of astrocyte lineage diversity in the murine ventral and dorsal forebrain
spellingShingle TGFβ-signaling and FOXG1-expression are a hallmark of astrocyte lineage diversity in the murine ventral and dorsal forebrain
Weise, Stefan Christopher
LINEAGE-TRACING
NEURAL DIFFERENTIATION
SILAC
TGFBR2-KNOCKOUT
ASTRCYTE-DIVERSITY
title_short TGFβ-signaling and FOXG1-expression are a hallmark of astrocyte lineage diversity in the murine ventral and dorsal forebrain
title_full TGFβ-signaling and FOXG1-expression are a hallmark of astrocyte lineage diversity in the murine ventral and dorsal forebrain
title_fullStr TGFβ-signaling and FOXG1-expression are a hallmark of astrocyte lineage diversity in the murine ventral and dorsal forebrain
title_full_unstemmed TGFβ-signaling and FOXG1-expression are a hallmark of astrocyte lineage diversity in the murine ventral and dorsal forebrain
title_sort TGFβ-signaling and FOXG1-expression are a hallmark of astrocyte lineage diversity in the murine ventral and dorsal forebrain
dc.creator.none.fl_str_mv Weise, Stefan Christopher
Villarreal, Alejandro
Heidrich, Stefanie
Dehghanian, Fariba
Schachtrup, Christian
Nestel, Sigrun
Schwarz, Jennifer
Thedieck, Kathrin
Vogel, Tanja
author Weise, Stefan Christopher
author_facet Weise, Stefan Christopher
Villarreal, Alejandro
Heidrich, Stefanie
Dehghanian, Fariba
Schachtrup, Christian
Nestel, Sigrun
Schwarz, Jennifer
Thedieck, Kathrin
Vogel, Tanja
author_role author
author2 Villarreal, Alejandro
Heidrich, Stefanie
Dehghanian, Fariba
Schachtrup, Christian
Nestel, Sigrun
Schwarz, Jennifer
Thedieck, Kathrin
Vogel, Tanja
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv LINEAGE-TRACING
NEURAL DIFFERENTIATION
SILAC
TGFBR2-KNOCKOUT
ASTRCYTE-DIVERSITY
topic LINEAGE-TRACING
NEURAL DIFFERENTIATION
SILAC
TGFBR2-KNOCKOUT
ASTRCYTE-DIVERSITY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Heterogeneous astrocyte populations are defined by diversity in cellular environment, progenitor identity or function. Yet, little is known about the extent of the heterogeneity and how this diversity is acquired during development. To investigate the impact of TGF (transforming growth factor) β-signaling on astrocyte development in the telencephalon we deleted the TGFBR2 (transforming growth factor beta receptor 2) in early neural progenitor cells in mice using a FOXG1 (forkhead box G1)-driven CRE-recombinase. We used quantitative proteomics to characterize TGFBR2-deficient cells derived from the mouse telencephalon and identified differential protein expression of the astrocyte proteins GFAP (glial fibrillary acidic protein) and MFGE8 (milk fat globule-EGF factor 8). Biochemical and histological investigations revealed distinct populations of astrocytes in the dorsal and ventral telencephalon marked by GFAP or MFGE8 protein expression. The two subtypes differed in their response to TGFβ-signaling. Impaired TGFβ-signaling affected numbers of GFAP astrocytes in the ventral telencephalon. In contrast, TGFβ reduced MFGE8-expression in astrocytes deriving from both regions. Additionally, lineage tracing revealed that both GFAP and MFGE8 astrocyte subtypes derived partly from FOXG1-expressing neural precursor cells.
Fil: Weise, Stefan Christopher. Universität Freiburg Im Breisgau; Alemania
Fil: Villarreal, Alejandro. Universität Freiburg Im Breisgau; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: Heidrich, Stefanie. Universität Freiburg Im Breisgau; Alemania
Fil: Dehghanian, Fariba. University Of Isfahan; Irán. Universität Freiburg Im Breisgau; Alemania
Fil: Schachtrup, Christian. Universität Freiburg Im Breisgau; Alemania
Fil: Nestel, Sigrun. Universität Freiburg Im Breisgau; Alemania
Fil: Schwarz, Jennifer. Universität Freiburg Im Breisgau; Alemania
Fil: Thedieck, Kathrin. Universität Oldenburg; Argentina. University of Groningen; Países Bajos
Fil: Vogel, Tanja. Universität Freiburg Im Breisgau; Alemania
description Heterogeneous astrocyte populations are defined by diversity in cellular environment, progenitor identity or function. Yet, little is known about the extent of the heterogeneity and how this diversity is acquired during development. To investigate the impact of TGF (transforming growth factor) β-signaling on astrocyte development in the telencephalon we deleted the TGFBR2 (transforming growth factor beta receptor 2) in early neural progenitor cells in mice using a FOXG1 (forkhead box G1)-driven CRE-recombinase. We used quantitative proteomics to characterize TGFBR2-deficient cells derived from the mouse telencephalon and identified differential protein expression of the astrocyte proteins GFAP (glial fibrillary acidic protein) and MFGE8 (milk fat globule-EGF factor 8). Biochemical and histological investigations revealed distinct populations of astrocytes in the dorsal and ventral telencephalon marked by GFAP or MFGE8 protein expression. The two subtypes differed in their response to TGFβ-signaling. Impaired TGFβ-signaling affected numbers of GFAP astrocytes in the ventral telencephalon. In contrast, TGFβ reduced MFGE8-expression in astrocytes deriving from both regions. Additionally, lineage tracing revealed that both GFAP and MFGE8 astrocyte subtypes derived partly from FOXG1-expressing neural precursor cells.
publishDate 2018
dc.date.none.fl_str_mv 2018-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/87973
Weise, Stefan Christopher; Villarreal, Alejandro; Heidrich, Stefanie; Dehghanian, Fariba; Schachtrup, Christian; et al.; TGFβ-signaling and FOXG1-expression are a hallmark of astrocyte lineage diversity in the murine ventral and dorsal forebrain; Frontiers Research Foundation; Frontiers in Cellular Neuroscience; 12; 448; 11-2018; 1-16
1662-5102
CONICET Digital
CONICET
url http://hdl.handle.net/11336/87973
identifier_str_mv Weise, Stefan Christopher; Villarreal, Alejandro; Heidrich, Stefanie; Dehghanian, Fariba; Schachtrup, Christian; et al.; TGFβ-signaling and FOXG1-expression are a hallmark of astrocyte lineage diversity in the murine ventral and dorsal forebrain; Frontiers Research Foundation; Frontiers in Cellular Neuroscience; 12; 448; 11-2018; 1-16
1662-5102
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/article/10.3389/fncel.2018.00448/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/fncel.2018.00448
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Research Foundation
publisher.none.fl_str_mv Frontiers Research Foundation
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.000565

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