Effect of paclitaxel in combination with the beta2 adrenergic agonist salbutamol on breast cancer MDA-MB-231 cell line growing in vivo: Mechanist of action
- Autores
- Jabloñski, Martina; Rivero, Ezequiel; Rodriguez, María Sol; Bruque, Carlos David; Aparicio, Evangelina; Bruzzone, Ariana; Pérez Piñero, Cecilia; Luthy, Isabel Alicia
- Año de publicación
- 2020
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- Breast cancer is the leading cancer death cause of women in Ar- gentina. Beta2 adrenergic receptors have been described in several breast cancer cell lines and tumors. We have previously described (SAIC 2016, abstract 723) that the beta2 adrenergic agonist sal- butamol exerted in vitro a synergic effect with paclitaxel inhibiting cell proliferation. The aim of the present study was to assess the in vivo effect of the combination of paclitaxel and salbutamol in the triple-negative MDA-MB-231 cell line growing in nude mice. Both salbutamol (daily sc 1.2 mg/kg/day) and paclitaxel (10 mg/kg/week, ip in two injections) significantly inhibited tumor growth. Moreover, their combination inhibited very significantly this parameter. For ex- ample, day 63 (mean ± SEM in mm2): control tumors: 46,5 ± 9,70, n=8; paclitaxel: 32.4 ± 6,40, n=7, p<0.05; salbutamol 29.3 ± 7.58, n=7, p<0.05. Paclitaxel + salbutamol 9.33 ± 2.45, n=9, p<0.0001 against control, p<0.05 against each individually. The coefficient of drug interaction indicated synergism since the value obtained is < 1 (0.010, p<0.0005 in interaction by two-way ANOVA). In order to assess the mechanism of action, several in vitro exper- iments were performed. Paclitaxel diminished cell viability (trypan blue essay), while salbutamol diminished pERK/ERK ratio (using WB) and the expression of cyclin D1 gene (RT-qPCR). Moreover, both drugs enhanced apoptosis (acridine orange ethidium bromide staining assay). In preliminary results, salbutamol completely re- versed the increase of MDR1 (ABCB1) expression induced by pacli- taxel. This could account for the synergic effect of both drugs. These results suggest that the doses of chemotherapeutic drugs adminis- tered to patients in some triple negative (claudin-low) tumors could be reduced in the presence of salbutamol, diminishing their toxicity.
Fil: Jabloñski, Martina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Rivero, Ezequiel. No especifíca;
Fil: Rodriguez, María Sol. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Bruque, Carlos David. Dirección Nacional de Instituto de Investigación.Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Aparicio, Evangelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Bruzzone, Ariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Pérez Piñero, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Luthy, Isabel Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Sociedad Argentina de Fisiología - Materia
-
PACLITAXEL
SALBUTAMOL
BETA ADRENERGIC
BREAST CANCER - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/158117
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Effect of paclitaxel in combination with the beta2 adrenergic agonist salbutamol on breast cancer MDA-MB-231 cell line growing in vivo: Mechanist of actionJabloñski, MartinaRivero, EzequielRodriguez, María SolBruque, Carlos DavidAparicio, EvangelinaBruzzone, ArianaPérez Piñero, CeciliaLuthy, Isabel AliciaPACLITAXELSALBUTAMOLBETA ADRENERGICBREAST CANCERhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Breast cancer is the leading cancer death cause of women in Ar- gentina. Beta2 adrenergic receptors have been described in several breast cancer cell lines and tumors. We have previously described (SAIC 2016, abstract 723) that the beta2 adrenergic agonist sal- butamol exerted in vitro a synergic effect with paclitaxel inhibiting cell proliferation. The aim of the present study was to assess the in vivo effect of the combination of paclitaxel and salbutamol in the triple-negative MDA-MB-231 cell line growing in nude mice. Both salbutamol (daily sc 1.2 mg/kg/day) and paclitaxel (10 mg/kg/week, ip in two injections) significantly inhibited tumor growth. Moreover, their combination inhibited very significantly this parameter. For ex- ample, day 63 (mean ± SEM in mm2): control tumors: 46,5 ± 9,70, n=8; paclitaxel: 32.4 ± 6,40, n=7, p<0.05; salbutamol 29.3 ± 7.58, n=7, p<0.05. Paclitaxel + salbutamol 9.33 ± 2.45, n=9, p<0.0001 against control, p<0.05 against each individually. The coefficient of drug interaction indicated synergism since the value obtained is < 1 (0.010, p<0.0005 in interaction by two-way ANOVA). In order to assess the mechanism of action, several in vitro exper- iments were performed. Paclitaxel diminished cell viability (trypan blue essay), while salbutamol diminished pERK/ERK ratio (using WB) and the expression of cyclin D1 gene (RT-qPCR). Moreover, both drugs enhanced apoptosis (acridine orange ethidium bromide staining assay). In preliminary results, salbutamol completely re- versed the increase of MDR1 (ABCB1) expression induced by pacli- taxel. This could account for the synergic effect of both drugs. These results suggest that the doses of chemotherapeutic drugs adminis- tered to patients in some triple negative (claudin-low) tumors could be reduced in the presence of salbutamol, diminishing their toxicity.Fil: Jabloñski, Martina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Rivero, Ezequiel. No especifíca;Fil: Rodriguez, María Sol. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Bruque, Carlos David. Dirección Nacional de Instituto de Investigación.Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Aparicio, Evangelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Bruzzone, Ariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Pérez Piñero, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Luthy, Isabel Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaLXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de FisiologíaMar del PlataArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de InmunologíaSociedad Argentina de FisiologíaFundación Revista Medicina2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/158117Effect of paclitaxel in combination with the beta2 adrenergic agonist salbutamol on breast cancer MDA-MB-231 cell line growing in vivo: Mechanist of action; LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2020; 127-1270025-76801669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.medicinabuenosaires.com/indices-de-2020/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:22Zoai:ri.conicet.gov.ar:11336/158117instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:23.282CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Effect of paclitaxel in combination with the beta2 adrenergic agonist salbutamol on breast cancer MDA-MB-231 cell line growing in vivo: Mechanist of action |
title |
Effect of paclitaxel in combination with the beta2 adrenergic agonist salbutamol on breast cancer MDA-MB-231 cell line growing in vivo: Mechanist of action |
spellingShingle |
Effect of paclitaxel in combination with the beta2 adrenergic agonist salbutamol on breast cancer MDA-MB-231 cell line growing in vivo: Mechanist of action Jabloñski, Martina PACLITAXEL SALBUTAMOL BETA ADRENERGIC BREAST CANCER |
title_short |
Effect of paclitaxel in combination with the beta2 adrenergic agonist salbutamol on breast cancer MDA-MB-231 cell line growing in vivo: Mechanist of action |
title_full |
Effect of paclitaxel in combination with the beta2 adrenergic agonist salbutamol on breast cancer MDA-MB-231 cell line growing in vivo: Mechanist of action |
title_fullStr |
Effect of paclitaxel in combination with the beta2 adrenergic agonist salbutamol on breast cancer MDA-MB-231 cell line growing in vivo: Mechanist of action |
title_full_unstemmed |
Effect of paclitaxel in combination with the beta2 adrenergic agonist salbutamol on breast cancer MDA-MB-231 cell line growing in vivo: Mechanist of action |
title_sort |
Effect of paclitaxel in combination with the beta2 adrenergic agonist salbutamol on breast cancer MDA-MB-231 cell line growing in vivo: Mechanist of action |
dc.creator.none.fl_str_mv |
Jabloñski, Martina Rivero, Ezequiel Rodriguez, María Sol Bruque, Carlos David Aparicio, Evangelina Bruzzone, Ariana Pérez Piñero, Cecilia Luthy, Isabel Alicia |
author |
Jabloñski, Martina |
author_facet |
Jabloñski, Martina Rivero, Ezequiel Rodriguez, María Sol Bruque, Carlos David Aparicio, Evangelina Bruzzone, Ariana Pérez Piñero, Cecilia Luthy, Isabel Alicia |
author_role |
author |
author2 |
Rivero, Ezequiel Rodriguez, María Sol Bruque, Carlos David Aparicio, Evangelina Bruzzone, Ariana Pérez Piñero, Cecilia Luthy, Isabel Alicia |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
PACLITAXEL SALBUTAMOL BETA ADRENERGIC BREAST CANCER |
topic |
PACLITAXEL SALBUTAMOL BETA ADRENERGIC BREAST CANCER |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.3 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Breast cancer is the leading cancer death cause of women in Ar- gentina. Beta2 adrenergic receptors have been described in several breast cancer cell lines and tumors. We have previously described (SAIC 2016, abstract 723) that the beta2 adrenergic agonist sal- butamol exerted in vitro a synergic effect with paclitaxel inhibiting cell proliferation. The aim of the present study was to assess the in vivo effect of the combination of paclitaxel and salbutamol in the triple-negative MDA-MB-231 cell line growing in nude mice. Both salbutamol (daily sc 1.2 mg/kg/day) and paclitaxel (10 mg/kg/week, ip in two injections) significantly inhibited tumor growth. Moreover, their combination inhibited very significantly this parameter. For ex- ample, day 63 (mean ± SEM in mm2): control tumors: 46,5 ± 9,70, n=8; paclitaxel: 32.4 ± 6,40, n=7, p<0.05; salbutamol 29.3 ± 7.58, n=7, p<0.05. Paclitaxel + salbutamol 9.33 ± 2.45, n=9, p<0.0001 against control, p<0.05 against each individually. The coefficient of drug interaction indicated synergism since the value obtained is < 1 (0.010, p<0.0005 in interaction by two-way ANOVA). In order to assess the mechanism of action, several in vitro exper- iments were performed. Paclitaxel diminished cell viability (trypan blue essay), while salbutamol diminished pERK/ERK ratio (using WB) and the expression of cyclin D1 gene (RT-qPCR). Moreover, both drugs enhanced apoptosis (acridine orange ethidium bromide staining assay). In preliminary results, salbutamol completely re- versed the increase of MDR1 (ABCB1) expression induced by pacli- taxel. This could account for the synergic effect of both drugs. These results suggest that the doses of chemotherapeutic drugs adminis- tered to patients in some triple negative (claudin-low) tumors could be reduced in the presence of salbutamol, diminishing their toxicity. Fil: Jabloñski, Martina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina Fil: Rivero, Ezequiel. No especifíca; Fil: Rodriguez, María Sol. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina Fil: Bruque, Carlos David. Dirección Nacional de Instituto de Investigación.Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Aparicio, Evangelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina Fil: Bruzzone, Ariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina Fil: Pérez Piñero, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina Fil: Luthy, Isabel Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología Mar del Plata Argentina Sociedad Argentina de Investigación Clínica Sociedad Argentina de Inmunología Sociedad Argentina de Fisiología |
description |
Breast cancer is the leading cancer death cause of women in Ar- gentina. Beta2 adrenergic receptors have been described in several breast cancer cell lines and tumors. We have previously described (SAIC 2016, abstract 723) that the beta2 adrenergic agonist sal- butamol exerted in vitro a synergic effect with paclitaxel inhibiting cell proliferation. The aim of the present study was to assess the in vivo effect of the combination of paclitaxel and salbutamol in the triple-negative MDA-MB-231 cell line growing in nude mice. Both salbutamol (daily sc 1.2 mg/kg/day) and paclitaxel (10 mg/kg/week, ip in two injections) significantly inhibited tumor growth. Moreover, their combination inhibited very significantly this parameter. For ex- ample, day 63 (mean ± SEM in mm2): control tumors: 46,5 ± 9,70, n=8; paclitaxel: 32.4 ± 6,40, n=7, p<0.05; salbutamol 29.3 ± 7.58, n=7, p<0.05. Paclitaxel + salbutamol 9.33 ± 2.45, n=9, p<0.0001 against control, p<0.05 against each individually. The coefficient of drug interaction indicated synergism since the value obtained is < 1 (0.010, p<0.0005 in interaction by two-way ANOVA). In order to assess the mechanism of action, several in vitro exper- iments were performed. Paclitaxel diminished cell viability (trypan blue essay), while salbutamol diminished pERK/ERK ratio (using WB) and the expression of cyclin D1 gene (RT-qPCR). Moreover, both drugs enhanced apoptosis (acridine orange ethidium bromide staining assay). In preliminary results, salbutamol completely re- versed the increase of MDR1 (ABCB1) expression induced by pacli- taxel. This could account for the synergic effect of both drugs. These results suggest that the doses of chemotherapeutic drugs adminis- tered to patients in some triple negative (claudin-low) tumors could be reduced in the presence of salbutamol, diminishing their toxicity. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Reunión Journal http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
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http://hdl.handle.net/11336/158117 Effect of paclitaxel in combination with the beta2 adrenergic agonist salbutamol on breast cancer MDA-MB-231 cell line growing in vivo: Mechanist of action; LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2020; 127-127 0025-7680 1669-9106 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/158117 |
identifier_str_mv |
Effect of paclitaxel in combination with the beta2 adrenergic agonist salbutamol on breast cancer MDA-MB-231 cell line growing in vivo: Mechanist of action; LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2020; 127-127 0025-7680 1669-9106 CONICET Digital CONICET |
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eng |
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eng |
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Fundación Revista Medicina |
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