Valepotriate Hydrines Isolated from an Anticonvulsant Fraction of Valeriana pavonii Poepp. & Endl

Autores
Giraldo, Sara E.; Rincón, Javier; Guerrero, Mario F.; López, Isabel; Jiménez, Ignacio; Marder, Nora Mariel; Wasowski, Cristina Lucia N.; Vergel, Nadezdha E.
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The present study deals with the isolation and identification of three valepotriate hydrines that are first reported in Valeriana pavonii Poepp. & Endl. (Valerianaceae), which were obtained from a dichloromethane fraction showing anticonvulsant activity in vivo. The isolation and purification of dichloromethane fraction was carried out by chromatographic techniques. The compounds were identified by comparison of their 1H and 13C NMR spectra with previously published data in scientific literature. Maximal electroshock seizure was used as in vivo pharmacological test, additionally in vitro GABA-A/BDZ-binding site studies were performed. Three valepotriate hydrines: valtrate acetoxyhydrine (1), valtrate isovaleroyloxyhydrine (2) and valtrate chlorohydrine (3), were isolated from a dichloromethane fraction that offered 90% protection against crisis-like tonic-clonic seizures in an in vivo pharmacological maximal electroshock seizure (MES) test in mice (35 mg/kg, p.o.). According to an in vitro GABA-A/BDZ binding site test, the mechanism of action for these compounds does not involve binding to the GABA-A receptor. These compounds are reported in this species for the first time. The valepotriate hydrines isolated from V. pavonii could be active metabolites of this species with anticonvulsant properties, however further in vivo an in vitro studies are required. Their molecular mechanisms of action are unrelated to the benzodiazepine binding site of the GABA-A receptor.
Fil: Giraldo, Sara E.. Universidad Nacional de Colombia; Colombia
Fil: Rincón, Javier. Universidad Nacional de Colombia; Colombia
Fil: Guerrero, Mario F.. Universidad Nacional de Colombia; Colombia
Fil: López, Isabel. Universidad de la Laguna; España
Fil: Jiménez, Ignacio. Universidad de la Laguna; España
Fil: Marder, Nora Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; Argentina
Fil: Wasowski, Cristina Lucia N.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; Argentina
Fil: Vergel, Nadezdha E.. Universidad Nacional de Colombia; Colombia
Materia
Anticonvulsant
Gaba a Receptor
Tonic-Clonic-Epilepsy
Valeriana
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/8514
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/8514
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Valepotriate Hydrines Isolated from an Anticonvulsant Fraction of Valeriana pavonii Poepp. & EndlGiraldo, Sara E.Rincón, JavierGuerrero, Mario F.López, IsabelJiménez, IgnacioMarder, Nora MarielWasowski, Cristina Lucia N.Vergel, Nadezdha E.AnticonvulsantGaba a ReceptorTonic-Clonic-EpilepsyValerianahttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3The present study deals with the isolation and identification of three valepotriate hydrines that are first reported in Valeriana pavonii Poepp. & Endl. (Valerianaceae), which were obtained from a dichloromethane fraction showing anticonvulsant activity in vivo. The isolation and purification of dichloromethane fraction was carried out by chromatographic techniques. The compounds were identified by comparison of their 1H and 13C NMR spectra with previously published data in scientific literature. Maximal electroshock seizure was used as in vivo pharmacological test, additionally in vitro GABA-A/BDZ-binding site studies were performed. Three valepotriate hydrines: valtrate acetoxyhydrine (1), valtrate isovaleroyloxyhydrine (2) and valtrate chlorohydrine (3), were isolated from a dichloromethane fraction that offered 90% protection against crisis-like tonic-clonic seizures in an in vivo pharmacological maximal electroshock seizure (MES) test in mice (35 mg/kg, p.o.). According to an in vitro GABA-A/BDZ binding site test, the mechanism of action for these compounds does not involve binding to the GABA-A receptor. These compounds are reported in this species for the first time. The valepotriate hydrines isolated from V. pavonii could be active metabolites of this species with anticonvulsant properties, however further in vivo an in vitro studies are required. Their molecular mechanisms of action are unrelated to the benzodiazepine binding site of the GABA-A receptor.Fil: Giraldo, Sara E.. Universidad Nacional de Colombia; ColombiaFil: Rincón, Javier. Universidad Nacional de Colombia; ColombiaFil: Guerrero, Mario F.. Universidad Nacional de Colombia; ColombiaFil: López, Isabel. Universidad de la Laguna; EspañaFil: Jiménez, Ignacio. Universidad de la Laguna; EspañaFil: Marder, Nora Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; ArgentinaFil: Wasowski, Cristina Lucia N.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; ArgentinaFil: Vergel, Nadezdha E.. Universidad Nacional de Colombia; ColombiaColegio Farmaceuticos Provincia de Buenos Aires2013-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/8514Giraldo, Sara E.; Rincón, Javier; Guerrero, Mario F.; López, Isabel; Jiménez, Ignacio; et al.; Valepotriate Hydrines Isolated from an Anticonvulsant Fraction of Valeriana pavonii Poepp. & Endl; Colegio Farmaceuticos Provincia de Buenos Aires; Latin American Journal of Pharmacy; 32; 8-2013; 1224-12300326-2383enginfo:eu-repo/semantics/altIdentifier/url/http://www.latamjpharm.org/previous_issue.php?vol=32&num=8info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:06:49Zoai:ri.conicet.gov.ar:11336/8514instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:06:50.038CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Valepotriate Hydrines Isolated from an Anticonvulsant Fraction of Valeriana pavonii Poepp. & Endl
title Valepotriate Hydrines Isolated from an Anticonvulsant Fraction of Valeriana pavonii Poepp. & Endl
spellingShingle Valepotriate Hydrines Isolated from an Anticonvulsant Fraction of Valeriana pavonii Poepp. & Endl
Giraldo, Sara E.
Anticonvulsant
Gaba a Receptor
Tonic-Clonic-Epilepsy
Valeriana
title_short Valepotriate Hydrines Isolated from an Anticonvulsant Fraction of Valeriana pavonii Poepp. & Endl
title_full Valepotriate Hydrines Isolated from an Anticonvulsant Fraction of Valeriana pavonii Poepp. & Endl
title_fullStr Valepotriate Hydrines Isolated from an Anticonvulsant Fraction of Valeriana pavonii Poepp. & Endl
title_full_unstemmed Valepotriate Hydrines Isolated from an Anticonvulsant Fraction of Valeriana pavonii Poepp. & Endl
title_sort Valepotriate Hydrines Isolated from an Anticonvulsant Fraction of Valeriana pavonii Poepp. & Endl
dc.creator.none.fl_str_mv Giraldo, Sara E.
Rincón, Javier
Guerrero, Mario F.
López, Isabel
Jiménez, Ignacio
Marder, Nora Mariel
Wasowski, Cristina Lucia N.
Vergel, Nadezdha E.
author Giraldo, Sara E.
author_facet Giraldo, Sara E.
Rincón, Javier
Guerrero, Mario F.
López, Isabel
Jiménez, Ignacio
Marder, Nora Mariel
Wasowski, Cristina Lucia N.
Vergel, Nadezdha E.
author_role author
author2 Rincón, Javier
Guerrero, Mario F.
López, Isabel
Jiménez, Ignacio
Marder, Nora Mariel
Wasowski, Cristina Lucia N.
Vergel, Nadezdha E.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Anticonvulsant
Gaba a Receptor
Tonic-Clonic-Epilepsy
Valeriana
topic Anticonvulsant
Gaba a Receptor
Tonic-Clonic-Epilepsy
Valeriana
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The present study deals with the isolation and identification of three valepotriate hydrines that are first reported in Valeriana pavonii Poepp. & Endl. (Valerianaceae), which were obtained from a dichloromethane fraction showing anticonvulsant activity in vivo. The isolation and purification of dichloromethane fraction was carried out by chromatographic techniques. The compounds were identified by comparison of their 1H and 13C NMR spectra with previously published data in scientific literature. Maximal electroshock seizure was used as in vivo pharmacological test, additionally in vitro GABA-A/BDZ-binding site studies were performed. Three valepotriate hydrines: valtrate acetoxyhydrine (1), valtrate isovaleroyloxyhydrine (2) and valtrate chlorohydrine (3), were isolated from a dichloromethane fraction that offered 90% protection against crisis-like tonic-clonic seizures in an in vivo pharmacological maximal electroshock seizure (MES) test in mice (35 mg/kg, p.o.). According to an in vitro GABA-A/BDZ binding site test, the mechanism of action for these compounds does not involve binding to the GABA-A receptor. These compounds are reported in this species for the first time. The valepotriate hydrines isolated from V. pavonii could be active metabolites of this species with anticonvulsant properties, however further in vivo an in vitro studies are required. Their molecular mechanisms of action are unrelated to the benzodiazepine binding site of the GABA-A receptor.
Fil: Giraldo, Sara E.. Universidad Nacional de Colombia; Colombia
Fil: Rincón, Javier. Universidad Nacional de Colombia; Colombia
Fil: Guerrero, Mario F.. Universidad Nacional de Colombia; Colombia
Fil: López, Isabel. Universidad de la Laguna; España
Fil: Jiménez, Ignacio. Universidad de la Laguna; España
Fil: Marder, Nora Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; Argentina
Fil: Wasowski, Cristina Lucia N.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; Argentina
Fil: Vergel, Nadezdha E.. Universidad Nacional de Colombia; Colombia
description The present study deals with the isolation and identification of three valepotriate hydrines that are first reported in Valeriana pavonii Poepp. & Endl. (Valerianaceae), which were obtained from a dichloromethane fraction showing anticonvulsant activity in vivo. The isolation and purification of dichloromethane fraction was carried out by chromatographic techniques. The compounds were identified by comparison of their 1H and 13C NMR spectra with previously published data in scientific literature. Maximal electroshock seizure was used as in vivo pharmacological test, additionally in vitro GABA-A/BDZ-binding site studies were performed. Three valepotriate hydrines: valtrate acetoxyhydrine (1), valtrate isovaleroyloxyhydrine (2) and valtrate chlorohydrine (3), were isolated from a dichloromethane fraction that offered 90% protection against crisis-like tonic-clonic seizures in an in vivo pharmacological maximal electroshock seizure (MES) test in mice (35 mg/kg, p.o.). According to an in vitro GABA-A/BDZ binding site test, the mechanism of action for these compounds does not involve binding to the GABA-A receptor. These compounds are reported in this species for the first time. The valepotriate hydrines isolated from V. pavonii could be active metabolites of this species with anticonvulsant properties, however further in vivo an in vitro studies are required. Their molecular mechanisms of action are unrelated to the benzodiazepine binding site of the GABA-A receptor.
publishDate 2013
dc.date.none.fl_str_mv 2013-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/8514
Giraldo, Sara E.; Rincón, Javier; Guerrero, Mario F.; López, Isabel; Jiménez, Ignacio; et al.; Valepotriate Hydrines Isolated from an Anticonvulsant Fraction of Valeriana pavonii Poepp. & Endl; Colegio Farmaceuticos Provincia de Buenos Aires; Latin American Journal of Pharmacy; 32; 8-2013; 1224-1230
0326-2383
url http://hdl.handle.net/11336/8514
identifier_str_mv Giraldo, Sara E.; Rincón, Javier; Guerrero, Mario F.; López, Isabel; Jiménez, Ignacio; et al.; Valepotriate Hydrines Isolated from an Anticonvulsant Fraction of Valeriana pavonii Poepp. & Endl; Colegio Farmaceuticos Provincia de Buenos Aires; Latin American Journal of Pharmacy; 32; 8-2013; 1224-1230
0326-2383
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.latamjpharm.org/previous_issue.php?vol=32&num=8
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Colegio Farmaceuticos Provincia de Buenos Aires
publisher.none.fl_str_mv Colegio Farmaceuticos Provincia de Buenos Aires
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269976369561600
score 13.13397

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