Tuba8 Drives Differentiation of Cortical Radial Glia into Apical Intermediate Progenitors by Tuning Modifications of Tubulin C Termini

Autores
Ramos, Susana I.; Makeyev, Eugene V.; Salierno, Marcelo Javier; Kodama, Takashi; Kawakami, Yasuhiko; Sahara, Setsuko
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Most adult neurons and glia originate from radial glial progenitors (RGs), a type of stem cell typically extending from the apical to the basal side of the developing cortex. Precise regulation of the choice between RG self-renewal and differentiation is critical for normal development, but the mechanisms underlying this transition remain elusive. We show that the non-canonical tubulin Tuba8, transiently expressed in cortical progenitors, drives differentiation of RGs into apical intermediate progenitors, a more restricted progenitor type lacking attachment to the basal lamina. This effect depends on the unique C-terminal sequence of Tuba8 that antagonizes tubulin tyrosination and Δ2 cleavage, two post-translational modifications (PTMs) essential for RG fiber maintenance and the switch between direct and indirect neurogenesis and ultimately distinct neuronal lineage outcomes. Our work uncovers an instructive role of a developmentally regulated tubulin isotype in progenitor differentiation and provides new insights into biological functions of the cellular tubulin PTM “code.” Radial glial progenitors of the developing mouse cortex differentiate into a more restricted progenitor type, apical intermediate progenitors. Ramos et al. find that Tuba8 drives this differentiation by tuning tyrosination and Δ2 cleavage, post-translational modifications of tubulin C termini, highlighting the functional significance of the “tubulin code” in cortical progenitor differentiation.
Fil: Ramos, Susana I.. King's College London; Reino Unido
Fil: Makeyev, Eugene V.. King's College London; Reino Unido
Fil: Salierno, Marcelo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina
Fil: Kodama, Takashi. University Johns Hopkins; Estados Unidos
Fil: Kawakami, Yasuhiko. University Johns Hopkins; Estados Unidos
Fil: Sahara, Setsuko. King's College London; Reino Unido
Materia
APICAL INTERMEDIATE PROGENITOR
CELL FATE
CORTICAL PROGENITOR
RADIAL GLIA
TUBULIN POST-TRANSLATIONAL MODIFICATION
TYROSINATION
Δ2-TUBULIN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/211433
Acceder
id CONICETDig_341f8e92665795785685d02d4ceb3854
oai_identifier_str oai:ri.conicet.gov.ar:11336/211433
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Tuba8 Drives Differentiation of Cortical Radial Glia into Apical Intermediate Progenitors by Tuning Modifications of Tubulin C TerminiRamos, Susana I.Makeyev, Eugene V.Salierno, Marcelo JavierKodama, TakashiKawakami, YasuhikoSahara, SetsukoAPICAL INTERMEDIATE PROGENITORCELL FATECORTICAL PROGENITORRADIAL GLIATUBULIN POST-TRANSLATIONAL MODIFICATIONTYROSINATIONΔ2-TUBULINhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Most adult neurons and glia originate from radial glial progenitors (RGs), a type of stem cell typically extending from the apical to the basal side of the developing cortex. Precise regulation of the choice between RG self-renewal and differentiation is critical for normal development, but the mechanisms underlying this transition remain elusive. We show that the non-canonical tubulin Tuba8, transiently expressed in cortical progenitors, drives differentiation of RGs into apical intermediate progenitors, a more restricted progenitor type lacking attachment to the basal lamina. This effect depends on the unique C-terminal sequence of Tuba8 that antagonizes tubulin tyrosination and Δ2 cleavage, two post-translational modifications (PTMs) essential for RG fiber maintenance and the switch between direct and indirect neurogenesis and ultimately distinct neuronal lineage outcomes. Our work uncovers an instructive role of a developmentally regulated tubulin isotype in progenitor differentiation and provides new insights into biological functions of the cellular tubulin PTM “code.” Radial glial progenitors of the developing mouse cortex differentiate into a more restricted progenitor type, apical intermediate progenitors. Ramos et al. find that Tuba8 drives this differentiation by tuning tyrosination and Δ2 cleavage, post-translational modifications of tubulin C termini, highlighting the functional significance of the “tubulin code” in cortical progenitor differentiation.Fil: Ramos, Susana I.. King's College London; Reino UnidoFil: Makeyev, Eugene V.. King's College London; Reino UnidoFil: Salierno, Marcelo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; ArgentinaFil: Kodama, Takashi. University Johns Hopkins; Estados UnidosFil: Kawakami, Yasuhiko. University Johns Hopkins; Estados UnidosFil: Sahara, Setsuko. King's College London; Reino UnidoCell Press2020-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/211433Ramos, Susana I.; Makeyev, Eugene V.; Salierno, Marcelo Javier; Kodama, Takashi; Kawakami, Yasuhiko; et al.; Tuba8 Drives Differentiation of Cortical Radial Glia into Apical Intermediate Progenitors by Tuning Modifications of Tubulin C Termini; Cell Press; Developmental Cell; 52; 4; 2-2020; 477-491.e81534-5807CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S153458072030068Xinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.devcel.2020.01.036info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-05T09:40:43Zoai:ri.conicet.gov.ar:11336/211433instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-05 09:40:43.891CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Tuba8 Drives Differentiation of Cortical Radial Glia into Apical Intermediate Progenitors by Tuning Modifications of Tubulin C Termini
title Tuba8 Drives Differentiation of Cortical Radial Glia into Apical Intermediate Progenitors by Tuning Modifications of Tubulin C Termini
spellingShingle Tuba8 Drives Differentiation of Cortical Radial Glia into Apical Intermediate Progenitors by Tuning Modifications of Tubulin C Termini
Ramos, Susana I.
APICAL INTERMEDIATE PROGENITOR
CELL FATE
CORTICAL PROGENITOR
RADIAL GLIA
TUBULIN POST-TRANSLATIONAL MODIFICATION
TYROSINATION
Δ2-TUBULIN
title_short Tuba8 Drives Differentiation of Cortical Radial Glia into Apical Intermediate Progenitors by Tuning Modifications of Tubulin C Termini
title_full Tuba8 Drives Differentiation of Cortical Radial Glia into Apical Intermediate Progenitors by Tuning Modifications of Tubulin C Termini
title_fullStr Tuba8 Drives Differentiation of Cortical Radial Glia into Apical Intermediate Progenitors by Tuning Modifications of Tubulin C Termini
title_full_unstemmed Tuba8 Drives Differentiation of Cortical Radial Glia into Apical Intermediate Progenitors by Tuning Modifications of Tubulin C Termini
title_sort Tuba8 Drives Differentiation of Cortical Radial Glia into Apical Intermediate Progenitors by Tuning Modifications of Tubulin C Termini
dc.creator.none.fl_str_mv Ramos, Susana I.
Makeyev, Eugene V.
Salierno, Marcelo Javier
Kodama, Takashi
Kawakami, Yasuhiko
Sahara, Setsuko
author Ramos, Susana I.
author_facet Ramos, Susana I.
Makeyev, Eugene V.
Salierno, Marcelo Javier
Kodama, Takashi
Kawakami, Yasuhiko
Sahara, Setsuko
author_role author
author2 Makeyev, Eugene V.
Salierno, Marcelo Javier
Kodama, Takashi
Kawakami, Yasuhiko
Sahara, Setsuko
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv APICAL INTERMEDIATE PROGENITOR
CELL FATE
CORTICAL PROGENITOR
RADIAL GLIA
TUBULIN POST-TRANSLATIONAL MODIFICATION
TYROSINATION
Δ2-TUBULIN
topic APICAL INTERMEDIATE PROGENITOR
CELL FATE
CORTICAL PROGENITOR
RADIAL GLIA
TUBULIN POST-TRANSLATIONAL MODIFICATION
TYROSINATION
Δ2-TUBULIN
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Most adult neurons and glia originate from radial glial progenitors (RGs), a type of stem cell typically extending from the apical to the basal side of the developing cortex. Precise regulation of the choice between RG self-renewal and differentiation is critical for normal development, but the mechanisms underlying this transition remain elusive. We show that the non-canonical tubulin Tuba8, transiently expressed in cortical progenitors, drives differentiation of RGs into apical intermediate progenitors, a more restricted progenitor type lacking attachment to the basal lamina. This effect depends on the unique C-terminal sequence of Tuba8 that antagonizes tubulin tyrosination and Δ2 cleavage, two post-translational modifications (PTMs) essential for RG fiber maintenance and the switch between direct and indirect neurogenesis and ultimately distinct neuronal lineage outcomes. Our work uncovers an instructive role of a developmentally regulated tubulin isotype in progenitor differentiation and provides new insights into biological functions of the cellular tubulin PTM “code.” Radial glial progenitors of the developing mouse cortex differentiate into a more restricted progenitor type, apical intermediate progenitors. Ramos et al. find that Tuba8 drives this differentiation by tuning tyrosination and Δ2 cleavage, post-translational modifications of tubulin C termini, highlighting the functional significance of the “tubulin code” in cortical progenitor differentiation.
Fil: Ramos, Susana I.. King's College London; Reino Unido
Fil: Makeyev, Eugene V.. King's College London; Reino Unido
Fil: Salierno, Marcelo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentina
Fil: Kodama, Takashi. University Johns Hopkins; Estados Unidos
Fil: Kawakami, Yasuhiko. University Johns Hopkins; Estados Unidos
Fil: Sahara, Setsuko. King's College London; Reino Unido
description Most adult neurons and glia originate from radial glial progenitors (RGs), a type of stem cell typically extending from the apical to the basal side of the developing cortex. Precise regulation of the choice between RG self-renewal and differentiation is critical for normal development, but the mechanisms underlying this transition remain elusive. We show that the non-canonical tubulin Tuba8, transiently expressed in cortical progenitors, drives differentiation of RGs into apical intermediate progenitors, a more restricted progenitor type lacking attachment to the basal lamina. This effect depends on the unique C-terminal sequence of Tuba8 that antagonizes tubulin tyrosination and Δ2 cleavage, two post-translational modifications (PTMs) essential for RG fiber maintenance and the switch between direct and indirect neurogenesis and ultimately distinct neuronal lineage outcomes. Our work uncovers an instructive role of a developmentally regulated tubulin isotype in progenitor differentiation and provides new insights into biological functions of the cellular tubulin PTM “code.” Radial glial progenitors of the developing mouse cortex differentiate into a more restricted progenitor type, apical intermediate progenitors. Ramos et al. find that Tuba8 drives this differentiation by tuning tyrosination and Δ2 cleavage, post-translational modifications of tubulin C termini, highlighting the functional significance of the “tubulin code” in cortical progenitor differentiation.
publishDate 2020
dc.date.none.fl_str_mv 2020-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/211433
Ramos, Susana I.; Makeyev, Eugene V.; Salierno, Marcelo Javier; Kodama, Takashi; Kawakami, Yasuhiko; et al.; Tuba8 Drives Differentiation of Cortical Radial Glia into Apical Intermediate Progenitors by Tuning Modifications of Tubulin C Termini; Cell Press; Developmental Cell; 52; 4; 2-2020; 477-491.e8
1534-5807
CONICET Digital
CONICET
url http://hdl.handle.net/11336/211433
identifier_str_mv Ramos, Susana I.; Makeyev, Eugene V.; Salierno, Marcelo Javier; Kodama, Takashi; Kawakami, Yasuhiko; et al.; Tuba8 Drives Differentiation of Cortical Radial Glia into Apical Intermediate Progenitors by Tuning Modifications of Tubulin C Termini; Cell Press; Developmental Cell; 52; 4; 2-2020; 477-491.e8
1534-5807
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S153458072030068X
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.devcel.2020.01.036
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cell Press
publisher.none.fl_str_mv Cell Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1847976955643691008
score 13.121305

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