Combination of cyclic voltammetry and single-particle Brownian dynamics methodology to evaluate the fluidity of phospholipid monolayers at polarized liquid/liquid interfaces

Autores
Juarez, Andrea Virginia; Juárez, Ana Valeria; Wilke, Natalia; Yudi, Lidia Mabel
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The present work shows, for the first time, the use of single-particle Brownian dynamics methodology, followed by optical microscopy, coupled with electrochemistry techniques to evaluate the fluidity of phospholipid films adsorbed at liquid/liquid interfaces. The combination of the two methods allows observing the movement of latex beads at the liquid/liquid interface modified by a phospholipid monolayer, with and without polarization by linear potential scans or potential steps. In this way, the effect of polarization on distearoylphosphatidylglycerol (DSPG), dimyristoylphosphatidylcholine (DMPC), and distearoylphosphatidylcholine (DSPC) monolayers, adsorbed at the water/1,2-dichloroethane interface, could be directly observed and diffusion coefficient values were derived. Lateral motilities of these particles can be correlated with the monolayer fluidity and solid character. A comparison with the behavior of the lipids at air/water interfaces is also included.
Fil: Juarez, Andrea Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Juárez, Ana Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Wilke, Natalia. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Yudi, Lidia Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Materia
LATEX MICROBEADS
LIQUID/LIQUID INTERFACES
MONOLAYERS FLUIDITY
PHOSPHOLIPIDS MONOLAYERS
SINGLE-PARTICLE BROWNIAN DYNAMICS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/85196
Acceder
id CONICETDig_3405ea7aa4d143fcc1b1952150997054
oai_identifier_str oai:ri.conicet.gov.ar:11336/85196
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Combination of cyclic voltammetry and single-particle Brownian dynamics methodology to evaluate the fluidity of phospholipid monolayers at polarized liquid/liquid interfacesJuarez, Andrea VirginiaJuárez, Ana ValeriaWilke, NataliaYudi, Lidia MabelLATEX MICROBEADSLIQUID/LIQUID INTERFACESMONOLAYERS FLUIDITYPHOSPHOLIPIDS MONOLAYERSSINGLE-PARTICLE BROWNIAN DYNAMICShttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The present work shows, for the first time, the use of single-particle Brownian dynamics methodology, followed by optical microscopy, coupled with electrochemistry techniques to evaluate the fluidity of phospholipid films adsorbed at liquid/liquid interfaces. The combination of the two methods allows observing the movement of latex beads at the liquid/liquid interface modified by a phospholipid monolayer, with and without polarization by linear potential scans or potential steps. In this way, the effect of polarization on distearoylphosphatidylglycerol (DSPG), dimyristoylphosphatidylcholine (DMPC), and distearoylphosphatidylcholine (DSPC) monolayers, adsorbed at the water/1,2-dichloroethane interface, could be directly observed and diffusion coefficient values were derived. Lateral motilities of these particles can be correlated with the monolayer fluidity and solid character. A comparison with the behavior of the lipids at air/water interfaces is also included.Fil: Juarez, Andrea Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Juárez, Ana Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Wilke, Natalia. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Yudi, Lidia Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaPergamon-Elsevier Science Ltd2018-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/85196Juarez, Andrea Virginia; Juárez, Ana Valeria; Wilke, Natalia; Yudi, Lidia Mabel; Combination of cyclic voltammetry and single-particle Brownian dynamics methodology to evaluate the fluidity of phospholipid monolayers at polarized liquid/liquid interfaces; Pergamon-Elsevier Science Ltd; Electrochimica Acta; 281; 8-2018; 611-6180013-4686CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0013468618311630info:eu-repo/semantics/altIdentifier/doi/10.1016/j.electacta.2018.05.118info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:53:08Zoai:ri.conicet.gov.ar:11336/85196instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:53:09.104CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Combination of cyclic voltammetry and single-particle Brownian dynamics methodology to evaluate the fluidity of phospholipid monolayers at polarized liquid/liquid interfaces
title Combination of cyclic voltammetry and single-particle Brownian dynamics methodology to evaluate the fluidity of phospholipid monolayers at polarized liquid/liquid interfaces
spellingShingle Combination of cyclic voltammetry and single-particle Brownian dynamics methodology to evaluate the fluidity of phospholipid monolayers at polarized liquid/liquid interfaces
Juarez, Andrea Virginia
LATEX MICROBEADS
LIQUID/LIQUID INTERFACES
MONOLAYERS FLUIDITY
PHOSPHOLIPIDS MONOLAYERS
SINGLE-PARTICLE BROWNIAN DYNAMICS
title_short Combination of cyclic voltammetry and single-particle Brownian dynamics methodology to evaluate the fluidity of phospholipid monolayers at polarized liquid/liquid interfaces
title_full Combination of cyclic voltammetry and single-particle Brownian dynamics methodology to evaluate the fluidity of phospholipid monolayers at polarized liquid/liquid interfaces
title_fullStr Combination of cyclic voltammetry and single-particle Brownian dynamics methodology to evaluate the fluidity of phospholipid monolayers at polarized liquid/liquid interfaces
title_full_unstemmed Combination of cyclic voltammetry and single-particle Brownian dynamics methodology to evaluate the fluidity of phospholipid monolayers at polarized liquid/liquid interfaces
title_sort Combination of cyclic voltammetry and single-particle Brownian dynamics methodology to evaluate the fluidity of phospholipid monolayers at polarized liquid/liquid interfaces
dc.creator.none.fl_str_mv Juarez, Andrea Virginia
Juárez, Ana Valeria
Wilke, Natalia
Yudi, Lidia Mabel
author Juarez, Andrea Virginia
author_facet Juarez, Andrea Virginia
Juárez, Ana Valeria
Wilke, Natalia
Yudi, Lidia Mabel
author_role author
author2 Juárez, Ana Valeria
Wilke, Natalia
Yudi, Lidia Mabel
author2_role author
author
author
dc.subject.none.fl_str_mv LATEX MICROBEADS
LIQUID/LIQUID INTERFACES
MONOLAYERS FLUIDITY
PHOSPHOLIPIDS MONOLAYERS
SINGLE-PARTICLE BROWNIAN DYNAMICS
topic LATEX MICROBEADS
LIQUID/LIQUID INTERFACES
MONOLAYERS FLUIDITY
PHOSPHOLIPIDS MONOLAYERS
SINGLE-PARTICLE BROWNIAN DYNAMICS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The present work shows, for the first time, the use of single-particle Brownian dynamics methodology, followed by optical microscopy, coupled with electrochemistry techniques to evaluate the fluidity of phospholipid films adsorbed at liquid/liquid interfaces. The combination of the two methods allows observing the movement of latex beads at the liquid/liquid interface modified by a phospholipid monolayer, with and without polarization by linear potential scans or potential steps. In this way, the effect of polarization on distearoylphosphatidylglycerol (DSPG), dimyristoylphosphatidylcholine (DMPC), and distearoylphosphatidylcholine (DSPC) monolayers, adsorbed at the water/1,2-dichloroethane interface, could be directly observed and diffusion coefficient values were derived. Lateral motilities of these particles can be correlated with the monolayer fluidity and solid character. A comparison with the behavior of the lipids at air/water interfaces is also included.
Fil: Juarez, Andrea Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Juárez, Ana Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Wilke, Natalia. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Yudi, Lidia Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
description The present work shows, for the first time, the use of single-particle Brownian dynamics methodology, followed by optical microscopy, coupled with electrochemistry techniques to evaluate the fluidity of phospholipid films adsorbed at liquid/liquid interfaces. The combination of the two methods allows observing the movement of latex beads at the liquid/liquid interface modified by a phospholipid monolayer, with and without polarization by linear potential scans or potential steps. In this way, the effect of polarization on distearoylphosphatidylglycerol (DSPG), dimyristoylphosphatidylcholine (DMPC), and distearoylphosphatidylcholine (DSPC) monolayers, adsorbed at the water/1,2-dichloroethane interface, could be directly observed and diffusion coefficient values were derived. Lateral motilities of these particles can be correlated with the monolayer fluidity and solid character. A comparison with the behavior of the lipids at air/water interfaces is also included.
publishDate 2018
dc.date.none.fl_str_mv 2018-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/85196
Juarez, Andrea Virginia; Juárez, Ana Valeria; Wilke, Natalia; Yudi, Lidia Mabel; Combination of cyclic voltammetry and single-particle Brownian dynamics methodology to evaluate the fluidity of phospholipid monolayers at polarized liquid/liquid interfaces; Pergamon-Elsevier Science Ltd; Electrochimica Acta; 281; 8-2018; 611-618
0013-4686
CONICET Digital
CONICET
url http://hdl.handle.net/11336/85196
identifier_str_mv Juarez, Andrea Virginia; Juárez, Ana Valeria; Wilke, Natalia; Yudi, Lidia Mabel; Combination of cyclic voltammetry and single-particle Brownian dynamics methodology to evaluate the fluidity of phospholipid monolayers at polarized liquid/liquid interfaces; Pergamon-Elsevier Science Ltd; Electrochimica Acta; 281; 8-2018; 611-618
0013-4686
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0013468618311630
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.electacta.2018.05.118
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269204145766400
score 13.13397

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