Uptake and Intracellular Trafficking of Superantigens in Dendritic Cells.

Autores
Ganem, María B.; de Marzi, Mauricio Cesar; Fernández-Lynch, María J.; Jancic, Carolina Cristina; Vermeulen, Elba Monica; Geffner, Jorge Raul; Mariuzza, Roy; Fernández, Marisa Mariel; Malchiodi, Emilio Luis
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bacterial superantigens (SAgs) are exotoxins produced mainly by Staphylococcus aureus and Streptococcus pyogenes that can cause toxic shock syndrome (TSS). According to current paradigm, SAgs interact directly and simultaneously with T cell receptor (TCR) on the T cell and MHC class II (MHC-II) on the antigen-presenting cell (APC), thereby circumventing intracellular processing to trigger T cell activation. Dendritic cells (DCs) are professional APCs that coat nearly all body surfaces and are the most probable candidate to interact with SAgs. We demonstrate that SAgs are taken up by mouse DCs without triggering DC maturation. SAgs were found in intracellular acidic compartment of DCs as biologically active molecules. Moreover, SAgs co-localized with EEA1, RAB-7 and LAMP-2, at different times, and were then recycled to the cell membrane. DCs loaded with SAgs are capable of triggering in vitro lymphocyte proliferation and, injected into mice, stimulate T cells bearing the proper TCR in draining lymph nodes. Transportation and trafficking of SAgs in DCs might increase the local concentration of these exotoxins where they will produce the highest effect by promoting their encounter with both MHC-II and TCR in lymph nodes, and may explain how just a few SAg molecules can induce the severe pathology associated with TSS
Fil: Ganem, María B.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral;
Fil: de Marzi, Mauricio Cesar. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Est.de la Inmunidad Humoral Prof.r.a.margni; Universidad Nacional de Lujan. Departamento de Cs.basicas;
Fil: Fernández-Lynch, María J.. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Est.de la Inmunidad Humoral Prof.r.a.margni;
Fil: Jancic, Carolina Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biologia y Medicina Experimental (i); Argentina;
Fil: Vermeulen, Elba Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biologia y Medicina Experimental (i); Argentina;
Fil: Geffner, Jorge Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biologia y Medicina Experimental (i); Argentina;
Fil: Mariuzza, Roy. INST FOR BIOSCIENCE AND BIOTECHNOLOGY RESEARCH;
Fil: Fernández, Marisa Mariel. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Est.de la Inmunidad Humoral Prof.r.a.margni;
Fil: Malchiodi, Emilio Luis. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Est.de la Inmunidad Humoral Prof.r.a.margni;
Materia
SUPERANTIGENOS
CELULAS DENDRITICAS
TCR
TRAFICO
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/1248
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/1248
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Uptake and Intracellular Trafficking of Superantigens in Dendritic Cells.Ganem, María B.de Marzi, Mauricio CesarFernández-Lynch, María J.Jancic, Carolina CristinaVermeulen, Elba MonicaGeffner, Jorge RaulMariuzza, RoyFernández, Marisa MarielMalchiodi, Emilio LuisSUPERANTIGENOSCELULAS DENDRITICASTCRTRAFICOhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Bacterial superantigens (SAgs) are exotoxins produced mainly by Staphylococcus aureus and Streptococcus pyogenes that can cause toxic shock syndrome (TSS). According to current paradigm, SAgs interact directly and simultaneously with T cell receptor (TCR) on the T cell and MHC class II (MHC-II) on the antigen-presenting cell (APC), thereby circumventing intracellular processing to trigger T cell activation. Dendritic cells (DCs) are professional APCs that coat nearly all body surfaces and are the most probable candidate to interact with SAgs. We demonstrate that SAgs are taken up by mouse DCs without triggering DC maturation. SAgs were found in intracellular acidic compartment of DCs as biologically active molecules. Moreover, SAgs co-localized with EEA1, RAB-7 and LAMP-2, at different times, and were then recycled to the cell membrane. DCs loaded with SAgs are capable of triggering in vitro lymphocyte proliferation and, injected into mice, stimulate T cells bearing the proper TCR in draining lymph nodes. Transportation and trafficking of SAgs in DCs might increase the local concentration of these exotoxins where they will produce the highest effect by promoting their encounter with both MHC-II and TCR in lymph nodes, and may explain how just a few SAg molecules can induce the severe pathology associated with TSSFil: Ganem, María B.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral;Fil: de Marzi, Mauricio Cesar. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Est.de la Inmunidad Humoral Prof.r.a.margni; Universidad Nacional de Lujan. Departamento de Cs.basicas;Fil: Fernández-Lynch, María J.. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Est.de la Inmunidad Humoral Prof.r.a.margni;Fil: Jancic, Carolina Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biologia y Medicina Experimental (i); Argentina;Fil: Vermeulen, Elba Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biologia y Medicina Experimental (i); Argentina;Fil: Geffner, Jorge Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biologia y Medicina Experimental (i); Argentina;Fil: Mariuzza, Roy. INST FOR BIOSCIENCE AND BIOTECHNOLOGY RESEARCH;Fil: Fernández, Marisa Mariel. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Est.de la Inmunidad Humoral Prof.r.a.margni;Fil: Malchiodi, Emilio Luis. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Est.de la Inmunidad Humoral Prof.r.a.margni;Public Library Science2013-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/1248Ganem, María B.; de Marzi, Mauricio Cesar; Fernández-Lynch, María J.; Jancic, Carolina Cristina; Vermeulen, Elba Monica; et al.; Uptake and Intracellular Trafficking of Superantigens in Dendritic Cells.; Public Library Science; Plos One; 8; 6; 6-2013; 1-141932-6203enginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0066244info:eu-repo/semantics/altIdentifier/url/http://www.plosone.org/article/fetchObject.action?uri=info%3Adoi%2F10.1371%2Fjournal.pone.0066244&representation=PDFinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:34:05Zoai:ri.conicet.gov.ar:11336/1248instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:34:05.437CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Uptake and Intracellular Trafficking of Superantigens in Dendritic Cells.
title Uptake and Intracellular Trafficking of Superantigens in Dendritic Cells.
spellingShingle Uptake and Intracellular Trafficking of Superantigens in Dendritic Cells.
Ganem, María B.
SUPERANTIGENOS
CELULAS DENDRITICAS
TCR
TRAFICO
title_short Uptake and Intracellular Trafficking of Superantigens in Dendritic Cells.
title_full Uptake and Intracellular Trafficking of Superantigens in Dendritic Cells.
title_fullStr Uptake and Intracellular Trafficking of Superantigens in Dendritic Cells.
title_full_unstemmed Uptake and Intracellular Trafficking of Superantigens in Dendritic Cells.
title_sort Uptake and Intracellular Trafficking of Superantigens in Dendritic Cells.
dc.creator.none.fl_str_mv Ganem, María B.
de Marzi, Mauricio Cesar
Fernández-Lynch, María J.
Jancic, Carolina Cristina
Vermeulen, Elba Monica
Geffner, Jorge Raul
Mariuzza, Roy
Fernández, Marisa Mariel
Malchiodi, Emilio Luis
author Ganem, María B.
author_facet Ganem, María B.
de Marzi, Mauricio Cesar
Fernández-Lynch, María J.
Jancic, Carolina Cristina
Vermeulen, Elba Monica
Geffner, Jorge Raul
Mariuzza, Roy
Fernández, Marisa Mariel
Malchiodi, Emilio Luis
author_role author
author2 de Marzi, Mauricio Cesar
Fernández-Lynch, María J.
Jancic, Carolina Cristina
Vermeulen, Elba Monica
Geffner, Jorge Raul
Mariuzza, Roy
Fernández, Marisa Mariel
Malchiodi, Emilio Luis
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv SUPERANTIGENOS
CELULAS DENDRITICAS
TCR
TRAFICO
topic SUPERANTIGENOS
CELULAS DENDRITICAS
TCR
TRAFICO
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Bacterial superantigens (SAgs) are exotoxins produced mainly by Staphylococcus aureus and Streptococcus pyogenes that can cause toxic shock syndrome (TSS). According to current paradigm, SAgs interact directly and simultaneously with T cell receptor (TCR) on the T cell and MHC class II (MHC-II) on the antigen-presenting cell (APC), thereby circumventing intracellular processing to trigger T cell activation. Dendritic cells (DCs) are professional APCs that coat nearly all body surfaces and are the most probable candidate to interact with SAgs. We demonstrate that SAgs are taken up by mouse DCs without triggering DC maturation. SAgs were found in intracellular acidic compartment of DCs as biologically active molecules. Moreover, SAgs co-localized with EEA1, RAB-7 and LAMP-2, at different times, and were then recycled to the cell membrane. DCs loaded with SAgs are capable of triggering in vitro lymphocyte proliferation and, injected into mice, stimulate T cells bearing the proper TCR in draining lymph nodes. Transportation and trafficking of SAgs in DCs might increase the local concentration of these exotoxins where they will produce the highest effect by promoting their encounter with both MHC-II and TCR in lymph nodes, and may explain how just a few SAg molecules can induce the severe pathology associated with TSS
Fil: Ganem, María B.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral;
Fil: de Marzi, Mauricio Cesar. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Est.de la Inmunidad Humoral Prof.r.a.margni; Universidad Nacional de Lujan. Departamento de Cs.basicas;
Fil: Fernández-Lynch, María J.. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Est.de la Inmunidad Humoral Prof.r.a.margni;
Fil: Jancic, Carolina Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biologia y Medicina Experimental (i); Argentina;
Fil: Vermeulen, Elba Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biologia y Medicina Experimental (i); Argentina;
Fil: Geffner, Jorge Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biologia y Medicina Experimental (i); Argentina;
Fil: Mariuzza, Roy. INST FOR BIOSCIENCE AND BIOTECHNOLOGY RESEARCH;
Fil: Fernández, Marisa Mariel. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Est.de la Inmunidad Humoral Prof.r.a.margni;
Fil: Malchiodi, Emilio Luis. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Est.de la Inmunidad Humoral Prof.r.a.margni;
description Bacterial superantigens (SAgs) are exotoxins produced mainly by Staphylococcus aureus and Streptococcus pyogenes that can cause toxic shock syndrome (TSS). According to current paradigm, SAgs interact directly and simultaneously with T cell receptor (TCR) on the T cell and MHC class II (MHC-II) on the antigen-presenting cell (APC), thereby circumventing intracellular processing to trigger T cell activation. Dendritic cells (DCs) are professional APCs that coat nearly all body surfaces and are the most probable candidate to interact with SAgs. We demonstrate that SAgs are taken up by mouse DCs without triggering DC maturation. SAgs were found in intracellular acidic compartment of DCs as biologically active molecules. Moreover, SAgs co-localized with EEA1, RAB-7 and LAMP-2, at different times, and were then recycled to the cell membrane. DCs loaded with SAgs are capable of triggering in vitro lymphocyte proliferation and, injected into mice, stimulate T cells bearing the proper TCR in draining lymph nodes. Transportation and trafficking of SAgs in DCs might increase the local concentration of these exotoxins where they will produce the highest effect by promoting their encounter with both MHC-II and TCR in lymph nodes, and may explain how just a few SAg molecules can induce the severe pathology associated with TSS
publishDate 2013
dc.date.none.fl_str_mv 2013-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/1248
Ganem, María B.; de Marzi, Mauricio Cesar; Fernández-Lynch, María J.; Jancic, Carolina Cristina; Vermeulen, Elba Monica; et al.; Uptake and Intracellular Trafficking of Superantigens in Dendritic Cells.; Public Library Science; Plos One; 8; 6; 6-2013; 1-14
1932-6203
url http://hdl.handle.net/11336/1248
identifier_str_mv Ganem, María B.; de Marzi, Mauricio Cesar; Fernández-Lynch, María J.; Jancic, Carolina Cristina; Vermeulen, Elba Monica; et al.; Uptake and Intracellular Trafficking of Superantigens in Dendritic Cells.; Public Library Science; Plos One; 8; 6; 6-2013; 1-14
1932-6203
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0066244
info:eu-repo/semantics/altIdentifier/url/http://www.plosone.org/article/fetchObject.action?uri=info%3Adoi%2F10.1371%2Fjournal.pone.0066244&representation=PDF
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library Science
publisher.none.fl_str_mv Public Library Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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