Uptake and Intracellular Trafficking of Superantigens in Dendritic Cells

Autores
Ganem, María Bernarda; de Marzi, Mauricio Cesar; Fernández Lynch, María Julieta; Jancic, Carolina Cristina; Vermeulen, Monica; Geffner, Jorge Raul; Mariuzza, Roy; Fernández, Marisa Mariel; Malchiodi, Emilio Luis
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bacterial superantigens (SAgs) are exotoxins produced mainly by Staphylococcus aureus and Streptococcus pyogenes that can cause toxic shock syndrome (TSS). According to current paradigm, SAgs interact directly and simultaneously with T cell receptor (TCR) on the T cell and MHC class II (MHC-II) on the antigen-presenting cell (APC), thereby circumventing intracellular processing to trigger T cell activation. Dendritic cells (DCs) are professional APCs that coat nearly all body surfaces and are the most probable candidate to interact with SAgs. We demonstrate that SAgs are taken up by mouse DCs without triggering DC maturation. SAgs were found in intracellular acidic compartment of DCs as biologically active molecules. Moreover, SAgs co-localized with EEA1, RAB-7 and LAMP-2, at different times, and were then recycled to the cell membrane. DCs loaded with SAgs are capable of triggering in vitro lymphocyte proliferation and, injected into mice, stimulate T cells bearing the proper TCR in draining lymph nodes. Transportation and trafficking of SAgs in DCs might increase the local concentration of these exotoxins where they will produce the highest effect by promoting their encounter with both MHC-II and TCR in lymph nodes, and may explain how just a few SAg molecules can induce the severe pathology associated with TSS.
Fil: Ganem, María Bernarda. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; Argentina
Fil: de Marzi, Mauricio Cesar. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; Argentina. Universidad Nacional de Lujan; Argentina
Fil: Fernández Lynch, María Julieta. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; Argentina
Fil: Jancic, Carolina Cristina. Academia Nacional de Medicina de Buenos Aires. Instituto de Invest. Hematologicas "mariano R. Castex". Departamento de Inmunologia y Medicina Experimental; Argentina
Fil: Vermeulen, Monica. Academia Nacional de Medicina de Buenos Aires. Instituto de Invest. Hematologicas "mariano R. Castex". Departamento de Inmunologia y Medicina Experimental. Div.de Inmunologia; Argentina
Fil: Geffner, Jorge Raul. Academia Nacional de Medicina de Buenos Aires. Instituto de Invest. Hematologicas "mariano R. Castex". Departamento de Inmunologia y Medicina Experimental. Div.de Inmunologia; Argentina
Fil: Mariuzza, Roy. University Of Maryland. Biotechnology Institute; Estados Unidos
Fil: Fernández, Marisa Mariel. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; Argentina
Fil: Malchiodi, Emilio Luis. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; Argentina
Materia
Dendritic cells
uptake
traffic
superantigens
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/8262
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/8262
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Uptake and Intracellular Trafficking of Superantigens in Dendritic CellsGanem, María Bernardade Marzi, Mauricio CesarFernández Lynch, María JulietaJancic, Carolina CristinaVermeulen, MonicaGeffner, Jorge RaulMariuzza, RoyFernández, Marisa MarielMalchiodi, Emilio LuisDendritic cellsuptaketrafficsuperantigenshttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Bacterial superantigens (SAgs) are exotoxins produced mainly by Staphylococcus aureus and Streptococcus pyogenes that can cause toxic shock syndrome (TSS). According to current paradigm, SAgs interact directly and simultaneously with T cell receptor (TCR) on the T cell and MHC class II (MHC-II) on the antigen-presenting cell (APC), thereby circumventing intracellular processing to trigger T cell activation. Dendritic cells (DCs) are professional APCs that coat nearly all body surfaces and are the most probable candidate to interact with SAgs. We demonstrate that SAgs are taken up by mouse DCs without triggering DC maturation. SAgs were found in intracellular acidic compartment of DCs as biologically active molecules. Moreover, SAgs co-localized with EEA1, RAB-7 and LAMP-2, at different times, and were then recycled to the cell membrane. DCs loaded with SAgs are capable of triggering in vitro lymphocyte proliferation and, injected into mice, stimulate T cells bearing the proper TCR in draining lymph nodes. Transportation and trafficking of SAgs in DCs might increase the local concentration of these exotoxins where they will produce the highest effect by promoting their encounter with both MHC-II and TCR in lymph nodes, and may explain how just a few SAg molecules can induce the severe pathology associated with TSS.Fil: Ganem, María Bernarda. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; ArgentinaFil: de Marzi, Mauricio Cesar. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; Argentina. Universidad Nacional de Lujan; ArgentinaFil: Fernández Lynch, María Julieta. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; ArgentinaFil: Jancic, Carolina Cristina. Academia Nacional de Medicina de Buenos Aires. Instituto de Invest. Hematologicas "mariano R. Castex". Departamento de Inmunologia y Medicina Experimental; ArgentinaFil: Vermeulen, Monica. Academia Nacional de Medicina de Buenos Aires. Instituto de Invest. Hematologicas "mariano R. Castex". Departamento de Inmunologia y Medicina Experimental. Div.de Inmunologia; ArgentinaFil: Geffner, Jorge Raul. Academia Nacional de Medicina de Buenos Aires. Instituto de Invest. Hematologicas "mariano R. Castex". Departamento de Inmunologia y Medicina Experimental. Div.de Inmunologia; ArgentinaFil: Mariuzza, Roy. University Of Maryland. Biotechnology Institute; Estados UnidosFil: Fernández, Marisa Mariel. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; ArgentinaFil: Malchiodi, Emilio Luis. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; ArgentinaPublic Library Of Science2013-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/8262Ganem, María Bernarda; de Marzi, Mauricio Cesar; Fernández Lynch, María Julieta; Jancic, Carolina Cristina; Vermeulen, Monica; et al.; Uptake and Intracellular Trafficking of Superantigens in Dendritic Cells; Public Library Of Science; Plos One; 8; 6; 6-2013; 1-121932-6203enginfo:eu-repo/semantics/altIdentifier/url/http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0066244info:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1371/journal.pone.0066244info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:38:08Zoai:ri.conicet.gov.ar:11336/8262instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:38:08.751CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Uptake and Intracellular Trafficking of Superantigens in Dendritic Cells
title Uptake and Intracellular Trafficking of Superantigens in Dendritic Cells
spellingShingle Uptake and Intracellular Trafficking of Superantigens in Dendritic Cells
Ganem, María Bernarda
Dendritic cells
uptake
traffic
superantigens
title_short Uptake and Intracellular Trafficking of Superantigens in Dendritic Cells
title_full Uptake and Intracellular Trafficking of Superantigens in Dendritic Cells
title_fullStr Uptake and Intracellular Trafficking of Superantigens in Dendritic Cells
title_full_unstemmed Uptake and Intracellular Trafficking of Superantigens in Dendritic Cells
title_sort Uptake and Intracellular Trafficking of Superantigens in Dendritic Cells
dc.creator.none.fl_str_mv Ganem, María Bernarda
de Marzi, Mauricio Cesar
Fernández Lynch, María Julieta
Jancic, Carolina Cristina
Vermeulen, Monica
Geffner, Jorge Raul
Mariuzza, Roy
Fernández, Marisa Mariel
Malchiodi, Emilio Luis
author Ganem, María Bernarda
author_facet Ganem, María Bernarda
de Marzi, Mauricio Cesar
Fernández Lynch, María Julieta
Jancic, Carolina Cristina
Vermeulen, Monica
Geffner, Jorge Raul
Mariuzza, Roy
Fernández, Marisa Mariel
Malchiodi, Emilio Luis
author_role author
author2 de Marzi, Mauricio Cesar
Fernández Lynch, María Julieta
Jancic, Carolina Cristina
Vermeulen, Monica
Geffner, Jorge Raul
Mariuzza, Roy
Fernández, Marisa Mariel
Malchiodi, Emilio Luis
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Dendritic cells
uptake
traffic
superantigens
topic Dendritic cells
uptake
traffic
superantigens
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Bacterial superantigens (SAgs) are exotoxins produced mainly by Staphylococcus aureus and Streptococcus pyogenes that can cause toxic shock syndrome (TSS). According to current paradigm, SAgs interact directly and simultaneously with T cell receptor (TCR) on the T cell and MHC class II (MHC-II) on the antigen-presenting cell (APC), thereby circumventing intracellular processing to trigger T cell activation. Dendritic cells (DCs) are professional APCs that coat nearly all body surfaces and are the most probable candidate to interact with SAgs. We demonstrate that SAgs are taken up by mouse DCs without triggering DC maturation. SAgs were found in intracellular acidic compartment of DCs as biologically active molecules. Moreover, SAgs co-localized with EEA1, RAB-7 and LAMP-2, at different times, and were then recycled to the cell membrane. DCs loaded with SAgs are capable of triggering in vitro lymphocyte proliferation and, injected into mice, stimulate T cells bearing the proper TCR in draining lymph nodes. Transportation and trafficking of SAgs in DCs might increase the local concentration of these exotoxins where they will produce the highest effect by promoting their encounter with both MHC-II and TCR in lymph nodes, and may explain how just a few SAg molecules can induce the severe pathology associated with TSS.
Fil: Ganem, María Bernarda. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; Argentina
Fil: de Marzi, Mauricio Cesar. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; Argentina. Universidad Nacional de Lujan; Argentina
Fil: Fernández Lynch, María Julieta. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; Argentina
Fil: Jancic, Carolina Cristina. Academia Nacional de Medicina de Buenos Aires. Instituto de Invest. Hematologicas "mariano R. Castex". Departamento de Inmunologia y Medicina Experimental; Argentina
Fil: Vermeulen, Monica. Academia Nacional de Medicina de Buenos Aires. Instituto de Invest. Hematologicas "mariano R. Castex". Departamento de Inmunologia y Medicina Experimental. Div.de Inmunologia; Argentina
Fil: Geffner, Jorge Raul. Academia Nacional de Medicina de Buenos Aires. Instituto de Invest. Hematologicas "mariano R. Castex". Departamento de Inmunologia y Medicina Experimental. Div.de Inmunologia; Argentina
Fil: Mariuzza, Roy. University Of Maryland. Biotechnology Institute; Estados Unidos
Fil: Fernández, Marisa Mariel. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; Argentina
Fil: Malchiodi, Emilio Luis. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; Argentina
description Bacterial superantigens (SAgs) are exotoxins produced mainly by Staphylococcus aureus and Streptococcus pyogenes that can cause toxic shock syndrome (TSS). According to current paradigm, SAgs interact directly and simultaneously with T cell receptor (TCR) on the T cell and MHC class II (MHC-II) on the antigen-presenting cell (APC), thereby circumventing intracellular processing to trigger T cell activation. Dendritic cells (DCs) are professional APCs that coat nearly all body surfaces and are the most probable candidate to interact with SAgs. We demonstrate that SAgs are taken up by mouse DCs without triggering DC maturation. SAgs were found in intracellular acidic compartment of DCs as biologically active molecules. Moreover, SAgs co-localized with EEA1, RAB-7 and LAMP-2, at different times, and were then recycled to the cell membrane. DCs loaded with SAgs are capable of triggering in vitro lymphocyte proliferation and, injected into mice, stimulate T cells bearing the proper TCR in draining lymph nodes. Transportation and trafficking of SAgs in DCs might increase the local concentration of these exotoxins where they will produce the highest effect by promoting their encounter with both MHC-II and TCR in lymph nodes, and may explain how just a few SAg molecules can induce the severe pathology associated with TSS.
publishDate 2013
dc.date.none.fl_str_mv 2013-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/8262
Ganem, María Bernarda; de Marzi, Mauricio Cesar; Fernández Lynch, María Julieta; Jancic, Carolina Cristina; Vermeulen, Monica; et al.; Uptake and Intracellular Trafficking of Superantigens in Dendritic Cells; Public Library Of Science; Plos One; 8; 6; 6-2013; 1-12
1932-6203
url http://hdl.handle.net/11336/8262
identifier_str_mv Ganem, María Bernarda; de Marzi, Mauricio Cesar; Fernández Lynch, María Julieta; Jancic, Carolina Cristina; Vermeulen, Monica; et al.; Uptake and Intracellular Trafficking of Superantigens in Dendritic Cells; Public Library Of Science; Plos One; 8; 6; 6-2013; 1-12
1932-6203
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0066244
info:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1371/journal.pone.0066244
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library Of Science
publisher.none.fl_str_mv Public Library Of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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