Sialic acids Neu5Ac and KDN in adipose tissue samples from individuals following habitual vegetarian or non-vegetarian dietary patterns
- Autores
- Guerrero Flores, Gerardo Nataniel; Pacheco, Fabio Juliano; Boskovic, Danilo S.; Pacheco, Sandaly O. S.; Zhang, Guangyu; Fraser, Gary E.; Miles, Fayth L.
- Año de publicación
- 2023
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Sialic acids (Sias) are a class of sugar molecules with a parent nine‑carbon neuraminic acid, generally present at the ends of carbohydrate chains, either attached to cellular surfaces or as secreted glycoconjugates. Given their position and structural diversity, Sias modulate a wide variety of biological processes. However, little is known about the role of Sias in human adipose tissue, or their implications for health and disease, particularly among individuals following different dietary patterns. The goal of this study was to measure N‑Acetylneuraminic acid (Neu5Ac), N‑Glycolylneuraminic acid (Neu5Gc), and 2‑keto‑3‑deoxy‑d‑glycero‑d‑galacto‑nononic acid (KDN) concentrations in adipose tissue samples from participants in the Adventist Health Study‑2 (AHS‑2) and to compare the abundance of these Sias in individuals following habitual, long‑term vegetarian or non‑vegetarian dietary patterns. A method was successfully developed for the extraction anddetection of Sias in adipose tissue. Sias levels were quantified in 52 vegans, 56 lacto‑vegetarians, and 48 non‑vegetarians using LC–MS/MS with Neu5Ac‑D‑1,2,3‑13 C 3 as an internal standard. Dietary groups were compared using linear regression. Vegans and lacto‑ovo‑vegetarians had significantly higher concentrations of Neu5Ac relative to non‑vegetarians. While KDN levels tended to be higher in vegans and lacto‑ovo‑vegetarians, these differences were not statistically significant. However, KDN levels were significantly inversely associated with body mass index. In contrast, Neu5Gc was not detectedin human adipose samples. It is plausible that different Neu5Ac concentrations in adipose tissues of vegetarians, compared to those of non‑vegetarians, reflect a difference in the baseline inflammatory status between the two groups. Epidemiologic studies examining levels of Sias in human adipose tissue and other biospecimens will help to further explore their roles in development and progression of inflammatory conditions and chronic diseases.
Fil: Guerrero Flores, Gerardo Nataniel. Universidad Adventista del Plata. Secretaría de Ciencia y Técnica. Centro Interdisciplinario de Investigaciones En Ciencias de la Salud y del Comportamiento.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Pacheco, Fabio Juliano. Universidad Adventista del Plata; Argentina
Fil: Boskovic, Danilo S.. Loma Linda University (llu);
Fil: Pacheco, Sandaly O. S.. Universidad Adventista del Plata. Secretaría de Ciencia y Técnica. Centro Interdisciplinario de Investigaciones En Ciencias de la Salud y del Comportamiento.; Argentina
Fil: Zhang, Guangyu. Loma Linda University (llu);
Fil: Fraser, Gary E.. Loma Linda University (llu);
Fil: Miles, Fayth L.. Loma Linda University (llu); - Materia
-
Sialic acids
Adipose tissue
Diet
LCMSMS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/262293
Ver los metadatos del registro completo
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Sialic acids Neu5Ac and KDN in adipose tissue samples from individuals following habitual vegetarian or non-vegetarian dietary patternsGuerrero Flores, Gerardo NatanielPacheco, Fabio JulianoBoskovic, Danilo S.Pacheco, Sandaly O. S.Zhang, GuangyuFraser, Gary E.Miles, Fayth L.Sialic acidsAdipose tissueDietLCMSMShttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Sialic acids (Sias) are a class of sugar molecules with a parent nine‑carbon neuraminic acid, generally present at the ends of carbohydrate chains, either attached to cellular surfaces or as secreted glycoconjugates. Given their position and structural diversity, Sias modulate a wide variety of biological processes. However, little is known about the role of Sias in human adipose tissue, or their implications for health and disease, particularly among individuals following different dietary patterns. The goal of this study was to measure N‑Acetylneuraminic acid (Neu5Ac), N‑Glycolylneuraminic acid (Neu5Gc), and 2‑keto‑3‑deoxy‑d‑glycero‑d‑galacto‑nononic acid (KDN) concentrations in adipose tissue samples from participants in the Adventist Health Study‑2 (AHS‑2) and to compare the abundance of these Sias in individuals following habitual, long‑term vegetarian or non‑vegetarian dietary patterns. A method was successfully developed for the extraction anddetection of Sias in adipose tissue. Sias levels were quantified in 52 vegans, 56 lacto‑vegetarians, and 48 non‑vegetarians using LC–MS/MS with Neu5Ac‑D‑1,2,3‑13 C 3 as an internal standard. Dietary groups were compared using linear regression. Vegans and lacto‑ovo‑vegetarians had significantly higher concentrations of Neu5Ac relative to non‑vegetarians. While KDN levels tended to be higher in vegans and lacto‑ovo‑vegetarians, these differences were not statistically significant. However, KDN levels were significantly inversely associated with body mass index. In contrast, Neu5Gc was not detectedin human adipose samples. It is plausible that different Neu5Ac concentrations in adipose tissues of vegetarians, compared to those of non‑vegetarians, reflect a difference in the baseline inflammatory status between the two groups. Epidemiologic studies examining levels of Sias in human adipose tissue and other biospecimens will help to further explore their roles in development and progression of inflammatory conditions and chronic diseases.Fil: Guerrero Flores, Gerardo Nataniel. Universidad Adventista del Plata. Secretaría de Ciencia y Técnica. Centro Interdisciplinario de Investigaciones En Ciencias de la Salud y del Comportamiento.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Pacheco, Fabio Juliano. Universidad Adventista del Plata; ArgentinaFil: Boskovic, Danilo S.. Loma Linda University (llu);Fil: Pacheco, Sandaly O. S.. Universidad Adventista del Plata. Secretaría de Ciencia y Técnica. Centro Interdisciplinario de Investigaciones En Ciencias de la Salud y del Comportamiento.; ArgentinaFil: Zhang, Guangyu. Loma Linda University (llu);Fil: Fraser, Gary E.. Loma Linda University (llu);Fil: Miles, Fayth L.. Loma Linda University (llu);Springer2023-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/262293Guerrero Flores, Gerardo Nataniel; Pacheco, Fabio Juliano; Boskovic, Danilo S.; Pacheco, Sandaly O. S.; Zhang, Guangyu; et al.; Sialic acids Neu5Ac and KDN in adipose tissue samples from individuals following habitual vegetarian or non-vegetarian dietary patterns; Springer; Scientific Reports; 13; 1; 8-2023; 1-112045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-023-38102-zinfo:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-023-38102-zinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:06:10Zoai:ri.conicet.gov.ar:11336/262293instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:06:10.934CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Sialic acids Neu5Ac and KDN in adipose tissue samples from individuals following habitual vegetarian or non-vegetarian dietary patterns |
title |
Sialic acids Neu5Ac and KDN in adipose tissue samples from individuals following habitual vegetarian or non-vegetarian dietary patterns |
spellingShingle |
Sialic acids Neu5Ac and KDN in adipose tissue samples from individuals following habitual vegetarian or non-vegetarian dietary patterns Guerrero Flores, Gerardo Nataniel Sialic acids Adipose tissue Diet LCMSMS |
title_short |
Sialic acids Neu5Ac and KDN in adipose tissue samples from individuals following habitual vegetarian or non-vegetarian dietary patterns |
title_full |
Sialic acids Neu5Ac and KDN in adipose tissue samples from individuals following habitual vegetarian or non-vegetarian dietary patterns |
title_fullStr |
Sialic acids Neu5Ac and KDN in adipose tissue samples from individuals following habitual vegetarian or non-vegetarian dietary patterns |
title_full_unstemmed |
Sialic acids Neu5Ac and KDN in adipose tissue samples from individuals following habitual vegetarian or non-vegetarian dietary patterns |
title_sort |
Sialic acids Neu5Ac and KDN in adipose tissue samples from individuals following habitual vegetarian or non-vegetarian dietary patterns |
dc.creator.none.fl_str_mv |
Guerrero Flores, Gerardo Nataniel Pacheco, Fabio Juliano Boskovic, Danilo S. Pacheco, Sandaly O. S. Zhang, Guangyu Fraser, Gary E. Miles, Fayth L. |
author |
Guerrero Flores, Gerardo Nataniel |
author_facet |
Guerrero Flores, Gerardo Nataniel Pacheco, Fabio Juliano Boskovic, Danilo S. Pacheco, Sandaly O. S. Zhang, Guangyu Fraser, Gary E. Miles, Fayth L. |
author_role |
author |
author2 |
Pacheco, Fabio Juliano Boskovic, Danilo S. Pacheco, Sandaly O. S. Zhang, Guangyu Fraser, Gary E. Miles, Fayth L. |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
Sialic acids Adipose tissue Diet LCMSMS |
topic |
Sialic acids Adipose tissue Diet LCMSMS |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.3 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Sialic acids (Sias) are a class of sugar molecules with a parent nine‑carbon neuraminic acid, generally present at the ends of carbohydrate chains, either attached to cellular surfaces or as secreted glycoconjugates. Given their position and structural diversity, Sias modulate a wide variety of biological processes. However, little is known about the role of Sias in human adipose tissue, or their implications for health and disease, particularly among individuals following different dietary patterns. The goal of this study was to measure N‑Acetylneuraminic acid (Neu5Ac), N‑Glycolylneuraminic acid (Neu5Gc), and 2‑keto‑3‑deoxy‑d‑glycero‑d‑galacto‑nononic acid (KDN) concentrations in adipose tissue samples from participants in the Adventist Health Study‑2 (AHS‑2) and to compare the abundance of these Sias in individuals following habitual, long‑term vegetarian or non‑vegetarian dietary patterns. A method was successfully developed for the extraction anddetection of Sias in adipose tissue. Sias levels were quantified in 52 vegans, 56 lacto‑vegetarians, and 48 non‑vegetarians using LC–MS/MS with Neu5Ac‑D‑1,2,3‑13 C 3 as an internal standard. Dietary groups were compared using linear regression. Vegans and lacto‑ovo‑vegetarians had significantly higher concentrations of Neu5Ac relative to non‑vegetarians. While KDN levels tended to be higher in vegans and lacto‑ovo‑vegetarians, these differences were not statistically significant. However, KDN levels were significantly inversely associated with body mass index. In contrast, Neu5Gc was not detectedin human adipose samples. It is plausible that different Neu5Ac concentrations in adipose tissues of vegetarians, compared to those of non‑vegetarians, reflect a difference in the baseline inflammatory status between the two groups. Epidemiologic studies examining levels of Sias in human adipose tissue and other biospecimens will help to further explore their roles in development and progression of inflammatory conditions and chronic diseases. Fil: Guerrero Flores, Gerardo Nataniel. Universidad Adventista del Plata. Secretaría de Ciencia y Técnica. Centro Interdisciplinario de Investigaciones En Ciencias de la Salud y del Comportamiento.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina Fil: Pacheco, Fabio Juliano. Universidad Adventista del Plata; Argentina Fil: Boskovic, Danilo S.. Loma Linda University (llu); Fil: Pacheco, Sandaly O. S.. Universidad Adventista del Plata. Secretaría de Ciencia y Técnica. Centro Interdisciplinario de Investigaciones En Ciencias de la Salud y del Comportamiento.; Argentina Fil: Zhang, Guangyu. Loma Linda University (llu); Fil: Fraser, Gary E.. Loma Linda University (llu); Fil: Miles, Fayth L.. Loma Linda University (llu); |
description |
Sialic acids (Sias) are a class of sugar molecules with a parent nine‑carbon neuraminic acid, generally present at the ends of carbohydrate chains, either attached to cellular surfaces or as secreted glycoconjugates. Given their position and structural diversity, Sias modulate a wide variety of biological processes. However, little is known about the role of Sias in human adipose tissue, or their implications for health and disease, particularly among individuals following different dietary patterns. The goal of this study was to measure N‑Acetylneuraminic acid (Neu5Ac), N‑Glycolylneuraminic acid (Neu5Gc), and 2‑keto‑3‑deoxy‑d‑glycero‑d‑galacto‑nononic acid (KDN) concentrations in adipose tissue samples from participants in the Adventist Health Study‑2 (AHS‑2) and to compare the abundance of these Sias in individuals following habitual, long‑term vegetarian or non‑vegetarian dietary patterns. A method was successfully developed for the extraction anddetection of Sias in adipose tissue. Sias levels were quantified in 52 vegans, 56 lacto‑vegetarians, and 48 non‑vegetarians using LC–MS/MS with Neu5Ac‑D‑1,2,3‑13 C 3 as an internal standard. Dietary groups were compared using linear regression. Vegans and lacto‑ovo‑vegetarians had significantly higher concentrations of Neu5Ac relative to non‑vegetarians. While KDN levels tended to be higher in vegans and lacto‑ovo‑vegetarians, these differences were not statistically significant. However, KDN levels were significantly inversely associated with body mass index. In contrast, Neu5Gc was not detectedin human adipose samples. It is plausible that different Neu5Ac concentrations in adipose tissues of vegetarians, compared to those of non‑vegetarians, reflect a difference in the baseline inflammatory status between the two groups. Epidemiologic studies examining levels of Sias in human adipose tissue and other biospecimens will help to further explore their roles in development and progression of inflammatory conditions and chronic diseases. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-08 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/262293 Guerrero Flores, Gerardo Nataniel; Pacheco, Fabio Juliano; Boskovic, Danilo S.; Pacheco, Sandaly O. S.; Zhang, Guangyu; et al.; Sialic acids Neu5Ac and KDN in adipose tissue samples from individuals following habitual vegetarian or non-vegetarian dietary patterns; Springer; Scientific Reports; 13; 1; 8-2023; 1-11 2045-2322 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/262293 |
identifier_str_mv |
Guerrero Flores, Gerardo Nataniel; Pacheco, Fabio Juliano; Boskovic, Danilo S.; Pacheco, Sandaly O. S.; Zhang, Guangyu; et al.; Sialic acids Neu5Ac and KDN in adipose tissue samples from individuals following habitual vegetarian or non-vegetarian dietary patterns; Springer; Scientific Reports; 13; 1; 8-2023; 1-11 2045-2322 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-023-38102-z info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-023-38102-z |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842269945535135744 |
score |
13.13397 |