Sialic acids Neu5Ac and KDN in adipose tissue samples from individuals following habitual vegetarian or non-vegetarian dietary patterns

Autores
Guerrero Flores, Gerardo Nataniel; Pacheco, Fabio Juliano; Boskovic, Danilo S.; Pacheco, Sandaly O. S.; Zhang, Guangyu; Fraser, Gary E.; Miles, Fayth L.
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Sialic acids (Sias) are a class of sugar molecules with a parent nine‑carbon neuraminic acid, generally present at the ends of carbohydrate chains, either attached to cellular surfaces or as secreted glycoconjugates. Given their position and structural diversity, Sias modulate a wide variety of biological processes. However, little is known about the role of Sias in human adipose tissue, or their implications for health and disease, particularly among individuals following different dietary patterns. The goal of this study was to measure N‑Acetylneuraminic acid (Neu5Ac), N‑Glycolylneuraminic acid (Neu5Gc), and 2‑keto‑3‑deoxy‑d‑glycero‑d‑galacto‑nononic acid (KDN) concentrations in adipose tissue samples from participants in the Adventist Health Study‑2 (AHS‑2) and to compare the abundance of these Sias in individuals following habitual, long‑term vegetarian or non‑vegetarian dietary patterns. A method was successfully developed for the extraction anddetection of Sias in adipose tissue. Sias levels were quantified in 52 vegans, 56 lacto‑vegetarians, and 48 non‑vegetarians using LC–MS/MS with Neu5Ac‑D‑1,2,3‑13 C 3 as an internal standard. Dietary groups were compared using linear regression. Vegans and lacto‑ovo‑vegetarians had significantly higher concentrations of Neu5Ac relative to non‑vegetarians. While KDN levels tended to be higher in vegans and lacto‑ovo‑vegetarians, these differences were not statistically significant. However, KDN levels were significantly inversely associated with body mass index. In contrast, Neu5Gc was not detectedin human adipose samples. It is plausible that different Neu5Ac concentrations in adipose tissues of vegetarians, compared to those of non‑vegetarians, reflect a difference in the baseline inflammatory status between the two groups. Epidemiologic studies examining levels of Sias in human adipose tissue and other biospecimens will help to further explore their roles in development and progression of inflammatory conditions and chronic diseases.
Fil: Guerrero Flores, Gerardo Nataniel. Universidad Adventista del Plata. Secretaría de Ciencia y Técnica. Centro Interdisciplinario de Investigaciones En Ciencias de la Salud y del Comportamiento.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Pacheco, Fabio Juliano. Universidad Adventista del Plata; Argentina
Fil: Boskovic, Danilo S.. Loma Linda University (llu);
Fil: Pacheco, Sandaly O. S.. Universidad Adventista del Plata. Secretaría de Ciencia y Técnica. Centro Interdisciplinario de Investigaciones En Ciencias de la Salud y del Comportamiento.; Argentina
Fil: Zhang, Guangyu. Loma Linda University (llu);
Fil: Fraser, Gary E.. Loma Linda University (llu);
Fil: Miles, Fayth L.. Loma Linda University (llu);
Materia
Sialic acids
Adipose tissue
Diet
LCMSMS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/262293

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network_name_str CONICET Digital (CONICET)
spelling Sialic acids Neu5Ac and KDN in adipose tissue samples from individuals following habitual vegetarian or non-vegetarian dietary patternsGuerrero Flores, Gerardo NatanielPacheco, Fabio JulianoBoskovic, Danilo S.Pacheco, Sandaly O. S.Zhang, GuangyuFraser, Gary E.Miles, Fayth L.Sialic acidsAdipose tissueDietLCMSMShttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Sialic acids (Sias) are a class of sugar molecules with a parent nine‑carbon neuraminic acid, generally present at the ends of carbohydrate chains, either attached to cellular surfaces or as secreted glycoconjugates. Given their position and structural diversity, Sias modulate a wide variety of biological processes. However, little is known about the role of Sias in human adipose tissue, or their implications for health and disease, particularly among individuals following different dietary patterns. The goal of this study was to measure N‑Acetylneuraminic acid (Neu5Ac), N‑Glycolylneuraminic acid (Neu5Gc), and 2‑keto‑3‑deoxy‑d‑glycero‑d‑galacto‑nononic acid (KDN) concentrations in adipose tissue samples from participants in the Adventist Health Study‑2 (AHS‑2) and to compare the abundance of these Sias in individuals following habitual, long‑term vegetarian or non‑vegetarian dietary patterns. A method was successfully developed for the extraction anddetection of Sias in adipose tissue. Sias levels were quantified in 52 vegans, 56 lacto‑vegetarians, and 48 non‑vegetarians using LC–MS/MS with Neu5Ac‑D‑1,2,3‑13 C 3 as an internal standard. Dietary groups were compared using linear regression. Vegans and lacto‑ovo‑vegetarians had significantly higher concentrations of Neu5Ac relative to non‑vegetarians. While KDN levels tended to be higher in vegans and lacto‑ovo‑vegetarians, these differences were not statistically significant. However, KDN levels were significantly inversely associated with body mass index. In contrast, Neu5Gc was not detectedin human adipose samples. It is plausible that different Neu5Ac concentrations in adipose tissues of vegetarians, compared to those of non‑vegetarians, reflect a difference in the baseline inflammatory status between the two groups. Epidemiologic studies examining levels of Sias in human adipose tissue and other biospecimens will help to further explore their roles in development and progression of inflammatory conditions and chronic diseases.Fil: Guerrero Flores, Gerardo Nataniel. Universidad Adventista del Plata. Secretaría de Ciencia y Técnica. Centro Interdisciplinario de Investigaciones En Ciencias de la Salud y del Comportamiento.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Pacheco, Fabio Juliano. Universidad Adventista del Plata; ArgentinaFil: Boskovic, Danilo S.. Loma Linda University (llu);Fil: Pacheco, Sandaly O. S.. Universidad Adventista del Plata. Secretaría de Ciencia y Técnica. Centro Interdisciplinario de Investigaciones En Ciencias de la Salud y del Comportamiento.; ArgentinaFil: Zhang, Guangyu. Loma Linda University (llu);Fil: Fraser, Gary E.. Loma Linda University (llu);Fil: Miles, Fayth L.. Loma Linda University (llu);Springer2023-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/262293Guerrero Flores, Gerardo Nataniel; Pacheco, Fabio Juliano; Boskovic, Danilo S.; Pacheco, Sandaly O. S.; Zhang, Guangyu; et al.; Sialic acids Neu5Ac and KDN in adipose tissue samples from individuals following habitual vegetarian or non-vegetarian dietary patterns; Springer; Scientific Reports; 13; 1; 8-2023; 1-112045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-023-38102-zinfo:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-023-38102-zinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:06:10Zoai:ri.conicet.gov.ar:11336/262293instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:06:10.934CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Sialic acids Neu5Ac and KDN in adipose tissue samples from individuals following habitual vegetarian or non-vegetarian dietary patterns
title Sialic acids Neu5Ac and KDN in adipose tissue samples from individuals following habitual vegetarian or non-vegetarian dietary patterns
spellingShingle Sialic acids Neu5Ac and KDN in adipose tissue samples from individuals following habitual vegetarian or non-vegetarian dietary patterns
Guerrero Flores, Gerardo Nataniel
Sialic acids
Adipose tissue
Diet
LCMSMS
title_short Sialic acids Neu5Ac and KDN in adipose tissue samples from individuals following habitual vegetarian or non-vegetarian dietary patterns
title_full Sialic acids Neu5Ac and KDN in adipose tissue samples from individuals following habitual vegetarian or non-vegetarian dietary patterns
title_fullStr Sialic acids Neu5Ac and KDN in adipose tissue samples from individuals following habitual vegetarian or non-vegetarian dietary patterns
title_full_unstemmed Sialic acids Neu5Ac and KDN in adipose tissue samples from individuals following habitual vegetarian or non-vegetarian dietary patterns
title_sort Sialic acids Neu5Ac and KDN in adipose tissue samples from individuals following habitual vegetarian or non-vegetarian dietary patterns
dc.creator.none.fl_str_mv Guerrero Flores, Gerardo Nataniel
Pacheco, Fabio Juliano
Boskovic, Danilo S.
Pacheco, Sandaly O. S.
Zhang, Guangyu
Fraser, Gary E.
Miles, Fayth L.
author Guerrero Flores, Gerardo Nataniel
author_facet Guerrero Flores, Gerardo Nataniel
Pacheco, Fabio Juliano
Boskovic, Danilo S.
Pacheco, Sandaly O. S.
Zhang, Guangyu
Fraser, Gary E.
Miles, Fayth L.
author_role author
author2 Pacheco, Fabio Juliano
Boskovic, Danilo S.
Pacheco, Sandaly O. S.
Zhang, Guangyu
Fraser, Gary E.
Miles, Fayth L.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Sialic acids
Adipose tissue
Diet
LCMSMS
topic Sialic acids
Adipose tissue
Diet
LCMSMS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Sialic acids (Sias) are a class of sugar molecules with a parent nine‑carbon neuraminic acid, generally present at the ends of carbohydrate chains, either attached to cellular surfaces or as secreted glycoconjugates. Given their position and structural diversity, Sias modulate a wide variety of biological processes. However, little is known about the role of Sias in human adipose tissue, or their implications for health and disease, particularly among individuals following different dietary patterns. The goal of this study was to measure N‑Acetylneuraminic acid (Neu5Ac), N‑Glycolylneuraminic acid (Neu5Gc), and 2‑keto‑3‑deoxy‑d‑glycero‑d‑galacto‑nononic acid (KDN) concentrations in adipose tissue samples from participants in the Adventist Health Study‑2 (AHS‑2) and to compare the abundance of these Sias in individuals following habitual, long‑term vegetarian or non‑vegetarian dietary patterns. A method was successfully developed for the extraction anddetection of Sias in adipose tissue. Sias levels were quantified in 52 vegans, 56 lacto‑vegetarians, and 48 non‑vegetarians using LC–MS/MS with Neu5Ac‑D‑1,2,3‑13 C 3 as an internal standard. Dietary groups were compared using linear regression. Vegans and lacto‑ovo‑vegetarians had significantly higher concentrations of Neu5Ac relative to non‑vegetarians. While KDN levels tended to be higher in vegans and lacto‑ovo‑vegetarians, these differences were not statistically significant. However, KDN levels were significantly inversely associated with body mass index. In contrast, Neu5Gc was not detectedin human adipose samples. It is plausible that different Neu5Ac concentrations in adipose tissues of vegetarians, compared to those of non‑vegetarians, reflect a difference in the baseline inflammatory status between the two groups. Epidemiologic studies examining levels of Sias in human adipose tissue and other biospecimens will help to further explore their roles in development and progression of inflammatory conditions and chronic diseases.
Fil: Guerrero Flores, Gerardo Nataniel. Universidad Adventista del Plata. Secretaría de Ciencia y Técnica. Centro Interdisciplinario de Investigaciones En Ciencias de la Salud y del Comportamiento.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Pacheco, Fabio Juliano. Universidad Adventista del Plata; Argentina
Fil: Boskovic, Danilo S.. Loma Linda University (llu);
Fil: Pacheco, Sandaly O. S.. Universidad Adventista del Plata. Secretaría de Ciencia y Técnica. Centro Interdisciplinario de Investigaciones En Ciencias de la Salud y del Comportamiento.; Argentina
Fil: Zhang, Guangyu. Loma Linda University (llu);
Fil: Fraser, Gary E.. Loma Linda University (llu);
Fil: Miles, Fayth L.. Loma Linda University (llu);
description Sialic acids (Sias) are a class of sugar molecules with a parent nine‑carbon neuraminic acid, generally present at the ends of carbohydrate chains, either attached to cellular surfaces or as secreted glycoconjugates. Given their position and structural diversity, Sias modulate a wide variety of biological processes. However, little is known about the role of Sias in human adipose tissue, or their implications for health and disease, particularly among individuals following different dietary patterns. The goal of this study was to measure N‑Acetylneuraminic acid (Neu5Ac), N‑Glycolylneuraminic acid (Neu5Gc), and 2‑keto‑3‑deoxy‑d‑glycero‑d‑galacto‑nononic acid (KDN) concentrations in adipose tissue samples from participants in the Adventist Health Study‑2 (AHS‑2) and to compare the abundance of these Sias in individuals following habitual, long‑term vegetarian or non‑vegetarian dietary patterns. A method was successfully developed for the extraction anddetection of Sias in adipose tissue. Sias levels were quantified in 52 vegans, 56 lacto‑vegetarians, and 48 non‑vegetarians using LC–MS/MS with Neu5Ac‑D‑1,2,3‑13 C 3 as an internal standard. Dietary groups were compared using linear regression. Vegans and lacto‑ovo‑vegetarians had significantly higher concentrations of Neu5Ac relative to non‑vegetarians. While KDN levels tended to be higher in vegans and lacto‑ovo‑vegetarians, these differences were not statistically significant. However, KDN levels were significantly inversely associated with body mass index. In contrast, Neu5Gc was not detectedin human adipose samples. It is plausible that different Neu5Ac concentrations in adipose tissues of vegetarians, compared to those of non‑vegetarians, reflect a difference in the baseline inflammatory status between the two groups. Epidemiologic studies examining levels of Sias in human adipose tissue and other biospecimens will help to further explore their roles in development and progression of inflammatory conditions and chronic diseases.
publishDate 2023
dc.date.none.fl_str_mv 2023-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/262293
Guerrero Flores, Gerardo Nataniel; Pacheco, Fabio Juliano; Boskovic, Danilo S.; Pacheco, Sandaly O. S.; Zhang, Guangyu; et al.; Sialic acids Neu5Ac and KDN in adipose tissue samples from individuals following habitual vegetarian or non-vegetarian dietary patterns; Springer; Scientific Reports; 13; 1; 8-2023; 1-11
2045-2322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/262293
identifier_str_mv Guerrero Flores, Gerardo Nataniel; Pacheco, Fabio Juliano; Boskovic, Danilo S.; Pacheco, Sandaly O. S.; Zhang, Guangyu; et al.; Sialic acids Neu5Ac and KDN in adipose tissue samples from individuals following habitual vegetarian or non-vegetarian dietary patterns; Springer; Scientific Reports; 13; 1; 8-2023; 1-11
2045-2322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-023-38102-z
info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-023-38102-z
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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