Protective Effects of New Medicinal Mushroom Grifola gargal Singer (Higher Basidiomycetes), on Induced DNA Damage in Somatic cells of Drosophila melanogaster
- Autores
- Postemsky, Pablo Daniel; Palermo, Ana María; Curvetto, Nestor Raul
- Año de publicación
- 2011
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Grifola gargal is an edible mushroom with attributed antioxidant properties. Different sources of G. gargal materials, i.e., fruit bodies and mycelia grown in liquid or solid media, were used to study its potential protective capacity when somatic mutation and recombination is induced in Drosophila melanogaster using DMBA (7-12-dimethyl-benz(α)anthracene) as promutagen. Heterozygote larvae (white/white+) were grown in media with different concentrations of DMBA. Grifola gargal fruit bodies (GgFB) or mycelia from liquid culture (GgLC) or from solid culture (GgWG), i.e., biotransformed wheat kernel flour, were added to the culture media in combined treatments with DMBA. Water, DMBA solvent, or wheat flour (WF) plus DMBA solvent were used as negative controls. Larval mortality increased from 9% to 11% in negative controls to 31% to 36% in DMBA treatments. The addition of GgFB, GgLC, or GgWG materials produced a protective effect on 25 μmol/vial DMBA-induced mortality. Mutations observed in SMART, as light spots per 100 eyes (LS/100 eyes), increased with increasing doses of DMBA; this was also true when considering the mutation incidence expressed as percentage of eyes exhibiting light spots (% eyes with LS). Interestingly, mycelia from GgFB, GgLC, or GgWG, in the presence of 25 μmol/vial DMBA, showed lower values in SMART of both the total LS/100 eyes and the percentage of eyes with LS. Thus, Grifola gargal materials were not only nontoxic, but in combination with 25 μmol/vial DMBA lowered the mortality induced by the promutagen and showed antimutagenic effects. Protective effects of G. gargal against DMBA are discussed in terms of the onset of desmutagenic and/or bioantimutagenic mechanisms of detoxification in the host organism, probably due to some bioactive compounds known to occur in higher mushrooms.
Fil: Postemsky, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; Argentina
Fil: Palermo, Ana María. Ministerio de Defensa. Instituto de Investigaciones Científicas y Técnicas Para la Defensa; Argentina
Fil: Curvetto, Nestor Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; Argentina - Materia
-
Somatic Mutation And Recombination Test
Gifola Gargal
Wild-Forest Edible Mushrooms - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/19279
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oai:ri.conicet.gov.ar:11336/19279 |
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3498 |
network_name_str |
CONICET Digital (CONICET) |
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Protective Effects of New Medicinal Mushroom Grifola gargal Singer (Higher Basidiomycetes), on Induced DNA Damage in Somatic cells of Drosophila melanogasterPostemsky, Pablo DanielPalermo, Ana MaríaCurvetto, Nestor RaulSomatic Mutation And Recombination TestGifola GargalWild-Forest Edible Mushroomshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Grifola gargal is an edible mushroom with attributed antioxidant properties. Different sources of G. gargal materials, i.e., fruit bodies and mycelia grown in liquid or solid media, were used to study its potential protective capacity when somatic mutation and recombination is induced in Drosophila melanogaster using DMBA (7-12-dimethyl-benz(α)anthracene) as promutagen. Heterozygote larvae (white/white+) were grown in media with different concentrations of DMBA. Grifola gargal fruit bodies (GgFB) or mycelia from liquid culture (GgLC) or from solid culture (GgWG), i.e., biotransformed wheat kernel flour, were added to the culture media in combined treatments with DMBA. Water, DMBA solvent, or wheat flour (WF) plus DMBA solvent were used as negative controls. Larval mortality increased from 9% to 11% in negative controls to 31% to 36% in DMBA treatments. The addition of GgFB, GgLC, or GgWG materials produced a protective effect on 25 μmol/vial DMBA-induced mortality. Mutations observed in SMART, as light spots per 100 eyes (LS/100 eyes), increased with increasing doses of DMBA; this was also true when considering the mutation incidence expressed as percentage of eyes exhibiting light spots (% eyes with LS). Interestingly, mycelia from GgFB, GgLC, or GgWG, in the presence of 25 μmol/vial DMBA, showed lower values in SMART of both the total LS/100 eyes and the percentage of eyes with LS. Thus, Grifola gargal materials were not only nontoxic, but in combination with 25 μmol/vial DMBA lowered the mortality induced by the promutagen and showed antimutagenic effects. Protective effects of G. gargal against DMBA are discussed in terms of the onset of desmutagenic and/or bioantimutagenic mechanisms of detoxification in the host organism, probably due to some bioactive compounds known to occur in higher mushrooms.Fil: Postemsky, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; ArgentinaFil: Palermo, Ana María. Ministerio de Defensa. Instituto de Investigaciones Científicas y Técnicas Para la Defensa; ArgentinaFil: Curvetto, Nestor Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; ArgentinaBegell House Inc2011-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/19279Postemsky, Pablo Daniel; Palermo, Ana María; Curvetto, Nestor Raul; Protective Effects of New Medicinal Mushroom Grifola gargal Singer (Higher Basidiomycetes), on Induced DNA Damage in Somatic cells of Drosophila melanogaster; Begell House Inc; International Journal Of Medicinal Mushrooms; 13; 6; 12-2011; 583-5941521-9437CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.dl.begellhouse.com/journals/708ae68d64b17c52,7b5e519241ca8193,311fb28f35fc39b4.htmlinfo:eu-repo/semantics/altIdentifier/doi/10.1615/IntJMedMushr.v13.i6.100info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:06:16Zoai:ri.conicet.gov.ar:11336/19279instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:06:16.519CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Protective Effects of New Medicinal Mushroom Grifola gargal Singer (Higher Basidiomycetes), on Induced DNA Damage in Somatic cells of Drosophila melanogaster |
title |
Protective Effects of New Medicinal Mushroom Grifola gargal Singer (Higher Basidiomycetes), on Induced DNA Damage in Somatic cells of Drosophila melanogaster |
spellingShingle |
Protective Effects of New Medicinal Mushroom Grifola gargal Singer (Higher Basidiomycetes), on Induced DNA Damage in Somatic cells of Drosophila melanogaster Postemsky, Pablo Daniel Somatic Mutation And Recombination Test Gifola Gargal Wild-Forest Edible Mushrooms |
title_short |
Protective Effects of New Medicinal Mushroom Grifola gargal Singer (Higher Basidiomycetes), on Induced DNA Damage in Somatic cells of Drosophila melanogaster |
title_full |
Protective Effects of New Medicinal Mushroom Grifola gargal Singer (Higher Basidiomycetes), on Induced DNA Damage in Somatic cells of Drosophila melanogaster |
title_fullStr |
Protective Effects of New Medicinal Mushroom Grifola gargal Singer (Higher Basidiomycetes), on Induced DNA Damage in Somatic cells of Drosophila melanogaster |
title_full_unstemmed |
Protective Effects of New Medicinal Mushroom Grifola gargal Singer (Higher Basidiomycetes), on Induced DNA Damage in Somatic cells of Drosophila melanogaster |
title_sort |
Protective Effects of New Medicinal Mushroom Grifola gargal Singer (Higher Basidiomycetes), on Induced DNA Damage in Somatic cells of Drosophila melanogaster |
dc.creator.none.fl_str_mv |
Postemsky, Pablo Daniel Palermo, Ana María Curvetto, Nestor Raul |
author |
Postemsky, Pablo Daniel |
author_facet |
Postemsky, Pablo Daniel Palermo, Ana María Curvetto, Nestor Raul |
author_role |
author |
author2 |
Palermo, Ana María Curvetto, Nestor Raul |
author2_role |
author author |
dc.subject.none.fl_str_mv |
Somatic Mutation And Recombination Test Gifola Gargal Wild-Forest Edible Mushrooms |
topic |
Somatic Mutation And Recombination Test Gifola Gargal Wild-Forest Edible Mushrooms |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Grifola gargal is an edible mushroom with attributed antioxidant properties. Different sources of G. gargal materials, i.e., fruit bodies and mycelia grown in liquid or solid media, were used to study its potential protective capacity when somatic mutation and recombination is induced in Drosophila melanogaster using DMBA (7-12-dimethyl-benz(α)anthracene) as promutagen. Heterozygote larvae (white/white+) were grown in media with different concentrations of DMBA. Grifola gargal fruit bodies (GgFB) or mycelia from liquid culture (GgLC) or from solid culture (GgWG), i.e., biotransformed wheat kernel flour, were added to the culture media in combined treatments with DMBA. Water, DMBA solvent, or wheat flour (WF) plus DMBA solvent were used as negative controls. Larval mortality increased from 9% to 11% in negative controls to 31% to 36% in DMBA treatments. The addition of GgFB, GgLC, or GgWG materials produced a protective effect on 25 μmol/vial DMBA-induced mortality. Mutations observed in SMART, as light spots per 100 eyes (LS/100 eyes), increased with increasing doses of DMBA; this was also true when considering the mutation incidence expressed as percentage of eyes exhibiting light spots (% eyes with LS). Interestingly, mycelia from GgFB, GgLC, or GgWG, in the presence of 25 μmol/vial DMBA, showed lower values in SMART of both the total LS/100 eyes and the percentage of eyes with LS. Thus, Grifola gargal materials were not only nontoxic, but in combination with 25 μmol/vial DMBA lowered the mortality induced by the promutagen and showed antimutagenic effects. Protective effects of G. gargal against DMBA are discussed in terms of the onset of desmutagenic and/or bioantimutagenic mechanisms of detoxification in the host organism, probably due to some bioactive compounds known to occur in higher mushrooms. Fil: Postemsky, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; Argentina Fil: Palermo, Ana María. Ministerio de Defensa. Instituto de Investigaciones Científicas y Técnicas Para la Defensa; Argentina Fil: Curvetto, Nestor Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; Argentina |
description |
Grifola gargal is an edible mushroom with attributed antioxidant properties. Different sources of G. gargal materials, i.e., fruit bodies and mycelia grown in liquid or solid media, were used to study its potential protective capacity when somatic mutation and recombination is induced in Drosophila melanogaster using DMBA (7-12-dimethyl-benz(α)anthracene) as promutagen. Heterozygote larvae (white/white+) were grown in media with different concentrations of DMBA. Grifola gargal fruit bodies (GgFB) or mycelia from liquid culture (GgLC) or from solid culture (GgWG), i.e., biotransformed wheat kernel flour, were added to the culture media in combined treatments with DMBA. Water, DMBA solvent, or wheat flour (WF) plus DMBA solvent were used as negative controls. Larval mortality increased from 9% to 11% in negative controls to 31% to 36% in DMBA treatments. The addition of GgFB, GgLC, or GgWG materials produced a protective effect on 25 μmol/vial DMBA-induced mortality. Mutations observed in SMART, as light spots per 100 eyes (LS/100 eyes), increased with increasing doses of DMBA; this was also true when considering the mutation incidence expressed as percentage of eyes exhibiting light spots (% eyes with LS). Interestingly, mycelia from GgFB, GgLC, or GgWG, in the presence of 25 μmol/vial DMBA, showed lower values in SMART of both the total LS/100 eyes and the percentage of eyes with LS. Thus, Grifola gargal materials were not only nontoxic, but in combination with 25 μmol/vial DMBA lowered the mortality induced by the promutagen and showed antimutagenic effects. Protective effects of G. gargal against DMBA are discussed in terms of the onset of desmutagenic and/or bioantimutagenic mechanisms of detoxification in the host organism, probably due to some bioactive compounds known to occur in higher mushrooms. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-12 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/19279 Postemsky, Pablo Daniel; Palermo, Ana María; Curvetto, Nestor Raul; Protective Effects of New Medicinal Mushroom Grifola gargal Singer (Higher Basidiomycetes), on Induced DNA Damage in Somatic cells of Drosophila melanogaster; Begell House Inc; International Journal Of Medicinal Mushrooms; 13; 6; 12-2011; 583-594 1521-9437 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/19279 |
identifier_str_mv |
Postemsky, Pablo Daniel; Palermo, Ana María; Curvetto, Nestor Raul; Protective Effects of New Medicinal Mushroom Grifola gargal Singer (Higher Basidiomycetes), on Induced DNA Damage in Somatic cells of Drosophila melanogaster; Begell House Inc; International Journal Of Medicinal Mushrooms; 13; 6; 12-2011; 583-594 1521-9437 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.dl.begellhouse.com/journals/708ae68d64b17c52,7b5e519241ca8193,311fb28f35fc39b4.html info:eu-repo/semantics/altIdentifier/doi/10.1615/IntJMedMushr.v13.i6.100 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Begell House Inc |
publisher.none.fl_str_mv |
Begell House Inc |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1842269950387945472 |
score |
13.13397 |