Metronomic doses and drug schematic combination response tested within chambered coverslips for the treatment of breast cancer cells (JIMT-1)

Autores
Rosero, Gustavo; Pattarone, Gisela; Peñaherrera Pazmiño, Ana Belén; Pilz, Julia; Bödecker, Joschka; Perez, Maximiliano Sebastian; Mertelsmann, Roland; Lerner, Betiana; Follo, Marie
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Low-dose metronomic (LDM) chemotherapy is an alternative to conventional chemotherapy and is the most frequently used approach in low dose chemotherapy regimens. The selection of patients, drug dosages, and dosing intervals in LDM is empirical. In this study, we systematically examined the schedule-dependent interaction of drugs on a breast cancer cell line (BCC) cultured in chambered coverslips. The LDM studies were combined with cell staining in order to better characterize different cell states and cell death modes, including caspase-dependent apoptosis, caspase-independent cell death and autophagy-dependent cell death. Microscope images were examined using the Fiji Trainable Weka Segmentation plugin to analyse cell area in 7500 images showing different modes of cell death. Paclitaxel combined with LDM chemotherapy demonstrated a reduction in the area covered by live cells. In contrast, there was an induction of high levels of cell death due to caspase-dependent apoptosis.
Fil: Rosero, Gustavo. Universidad Tecnológica Nacional; Argentina. Albert Ludwigs University of Freiburg; Alemania
Fil: Pattarone, Gisela. Albert Ludwigs University of Freiburg; Alemania
Fil: Peñaherrera Pazmiño, Ana Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Tecnológica Nacional; Argentina. Albert Ludwigs University of Freiburg; Alemania
Fil: Pilz, Julia. Albert Ludwigs University of Freiburg; Alemania
Fil: Bödecker, Joschka. Albert Ludwigs University of Freiburg; Alemania
Fil: Perez, Maximiliano Sebastian. Universidad de Buenos Aires; Argentina. Universidad Tecnológica Nacional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mertelsmann, Roland. Albert Ludwigs University of Freiburg; Alemania
Fil: Lerner, Betiana. Universidad de Buenos Aires; Argentina. Universidad Tecnológica Nacional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Follo, Marie. Universidad de Buenos Aires; Argentina
Materia
metronomic
chip
pdms
cancer
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/223536
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/223536
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Metronomic doses and drug schematic combination response tested within chambered coverslips for the treatment of breast cancer cells (JIMT-1)Rosero, GustavoPattarone, GiselaPeñaherrera Pazmiño, Ana BelénPilz, JuliaBödecker, JoschkaPerez, Maximiliano SebastianMertelsmann, RolandLerner, BetianaFollo, Mariemetronomicchippdmscancerhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Low-dose metronomic (LDM) chemotherapy is an alternative to conventional chemotherapy and is the most frequently used approach in low dose chemotherapy regimens. The selection of patients, drug dosages, and dosing intervals in LDM is empirical. In this study, we systematically examined the schedule-dependent interaction of drugs on a breast cancer cell line (BCC) cultured in chambered coverslips. The LDM studies were combined with cell staining in order to better characterize different cell states and cell death modes, including caspase-dependent apoptosis, caspase-independent cell death and autophagy-dependent cell death. Microscope images were examined using the Fiji Trainable Weka Segmentation plugin to analyse cell area in 7500 images showing different modes of cell death. Paclitaxel combined with LDM chemotherapy demonstrated a reduction in the area covered by live cells. In contrast, there was an induction of high levels of cell death due to caspase-dependent apoptosis.Fil: Rosero, Gustavo. Universidad Tecnológica Nacional; Argentina. Albert Ludwigs University of Freiburg; AlemaniaFil: Pattarone, Gisela. Albert Ludwigs University of Freiburg; AlemaniaFil: Peñaherrera Pazmiño, Ana Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Tecnológica Nacional; Argentina. Albert Ludwigs University of Freiburg; AlemaniaFil: Pilz, Julia. Albert Ludwigs University of Freiburg; AlemaniaFil: Bödecker, Joschka. Albert Ludwigs University of Freiburg; AlemaniaFil: Perez, Maximiliano Sebastian. Universidad de Buenos Aires; Argentina. Universidad Tecnológica Nacional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Mertelsmann, Roland. Albert Ludwigs University of Freiburg; AlemaniaFil: Lerner, Betiana. Universidad de Buenos Aires; Argentina. Universidad Tecnológica Nacional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Follo, Marie. Universidad de Buenos Aires; ArgentinaPublic Library of Science2022-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/223536Rosero, Gustavo; Pattarone, Gisela; Peñaherrera Pazmiño, Ana Belén; Pilz, Julia; Bödecker, Joschka; et al.; Metronomic doses and drug schematic combination response tested within chambered coverslips for the treatment of breast cancer cells (JIMT-1); Public Library of Science; Plos One; 17; 9-2022; 1-121932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0274911info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:20:49Zoai:ri.conicet.gov.ar:11336/223536instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:20:49.885CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Metronomic doses and drug schematic combination response tested within chambered coverslips for the treatment of breast cancer cells (JIMT-1)
title Metronomic doses and drug schematic combination response tested within chambered coverslips for the treatment of breast cancer cells (JIMT-1)
spellingShingle Metronomic doses and drug schematic combination response tested within chambered coverslips for the treatment of breast cancer cells (JIMT-1)
Rosero, Gustavo
metronomic
chip
pdms
cancer
title_short Metronomic doses and drug schematic combination response tested within chambered coverslips for the treatment of breast cancer cells (JIMT-1)
title_full Metronomic doses and drug schematic combination response tested within chambered coverslips for the treatment of breast cancer cells (JIMT-1)
title_fullStr Metronomic doses and drug schematic combination response tested within chambered coverslips for the treatment of breast cancer cells (JIMT-1)
title_full_unstemmed Metronomic doses and drug schematic combination response tested within chambered coverslips for the treatment of breast cancer cells (JIMT-1)
title_sort Metronomic doses and drug schematic combination response tested within chambered coverslips for the treatment of breast cancer cells (JIMT-1)
dc.creator.none.fl_str_mv Rosero, Gustavo
Pattarone, Gisela
Peñaherrera Pazmiño, Ana Belén
Pilz, Julia
Bödecker, Joschka
Perez, Maximiliano Sebastian
Mertelsmann, Roland
Lerner, Betiana
Follo, Marie
author Rosero, Gustavo
author_facet Rosero, Gustavo
Pattarone, Gisela
Peñaherrera Pazmiño, Ana Belén
Pilz, Julia
Bödecker, Joschka
Perez, Maximiliano Sebastian
Mertelsmann, Roland
Lerner, Betiana
Follo, Marie
author_role author
author2 Pattarone, Gisela
Peñaherrera Pazmiño, Ana Belén
Pilz, Julia
Bödecker, Joschka
Perez, Maximiliano Sebastian
Mertelsmann, Roland
Lerner, Betiana
Follo, Marie
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv metronomic
chip
pdms
cancer
topic metronomic
chip
pdms
cancer
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Low-dose metronomic (LDM) chemotherapy is an alternative to conventional chemotherapy and is the most frequently used approach in low dose chemotherapy regimens. The selection of patients, drug dosages, and dosing intervals in LDM is empirical. In this study, we systematically examined the schedule-dependent interaction of drugs on a breast cancer cell line (BCC) cultured in chambered coverslips. The LDM studies were combined with cell staining in order to better characterize different cell states and cell death modes, including caspase-dependent apoptosis, caspase-independent cell death and autophagy-dependent cell death. Microscope images were examined using the Fiji Trainable Weka Segmentation plugin to analyse cell area in 7500 images showing different modes of cell death. Paclitaxel combined with LDM chemotherapy demonstrated a reduction in the area covered by live cells. In contrast, there was an induction of high levels of cell death due to caspase-dependent apoptosis.
Fil: Rosero, Gustavo. Universidad Tecnológica Nacional; Argentina. Albert Ludwigs University of Freiburg; Alemania
Fil: Pattarone, Gisela. Albert Ludwigs University of Freiburg; Alemania
Fil: Peñaherrera Pazmiño, Ana Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Tecnológica Nacional; Argentina. Albert Ludwigs University of Freiburg; Alemania
Fil: Pilz, Julia. Albert Ludwigs University of Freiburg; Alemania
Fil: Bödecker, Joschka. Albert Ludwigs University of Freiburg; Alemania
Fil: Perez, Maximiliano Sebastian. Universidad de Buenos Aires; Argentina. Universidad Tecnológica Nacional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mertelsmann, Roland. Albert Ludwigs University of Freiburg; Alemania
Fil: Lerner, Betiana. Universidad de Buenos Aires; Argentina. Universidad Tecnológica Nacional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Follo, Marie. Universidad de Buenos Aires; Argentina
description Low-dose metronomic (LDM) chemotherapy is an alternative to conventional chemotherapy and is the most frequently used approach in low dose chemotherapy regimens. The selection of patients, drug dosages, and dosing intervals in LDM is empirical. In this study, we systematically examined the schedule-dependent interaction of drugs on a breast cancer cell line (BCC) cultured in chambered coverslips. The LDM studies were combined with cell staining in order to better characterize different cell states and cell death modes, including caspase-dependent apoptosis, caspase-independent cell death and autophagy-dependent cell death. Microscope images were examined using the Fiji Trainable Weka Segmentation plugin to analyse cell area in 7500 images showing different modes of cell death. Paclitaxel combined with LDM chemotherapy demonstrated a reduction in the area covered by live cells. In contrast, there was an induction of high levels of cell death due to caspase-dependent apoptosis.
publishDate 2022
dc.date.none.fl_str_mv 2022-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/223536
Rosero, Gustavo; Pattarone, Gisela; Peñaherrera Pazmiño, Ana Belén; Pilz, Julia; Bödecker, Joschka; et al.; Metronomic doses and drug schematic combination response tested within chambered coverslips for the treatment of breast cancer cells (JIMT-1); Public Library of Science; Plos One; 17; 9-2022; 1-12
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/223536
identifier_str_mv Rosero, Gustavo; Pattarone, Gisela; Peñaherrera Pazmiño, Ana Belén; Pilz, Julia; Bödecker, Joschka; et al.; Metronomic doses and drug schematic combination response tested within chambered coverslips for the treatment of breast cancer cells (JIMT-1); Public Library of Science; Plos One; 17; 9-2022; 1-12
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0274911
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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