Myelin Basic Protein and a Multiple Sclerosis-related MBP-peptide Bind to Oligonucleotides

Autores
Rozenblum, Tomas Guido; Kaufman, Tomás; Vitullo, Alfredo Daniel
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aptamer ligands for myelin basic protein (MBP) were obtained using the systematic evolution of ligand by exponential enrichment (SELEX) method. Two clones were isolated from a pool of oligonucleotides and tested for MBP targeting. Using purified MBP, we demonstrated the binding activity of the aptamers and we also showed the affinity of MBP for oligonucleotides of specific length. Moreover, one selected aptamer competitively inhibited the binding of an MBP-specific antibody to MBP and the aptamer was found more sensitive than a commercial antibody. In addition, we showed the ability of the aptamer to detect myelin-rich regions in paraffin-embedded mouse brain tissue. Therefore, the MBP-binding activity of the selected oligonucleotide may prove useful as a tool for life science and medical research for myelin detection and might be a good lead for testing it in autoimmune diseases such as multiple sclerosis.
Fil: Rozenblum, Tomas Guido. Universidad Maimonides. Facultad de Ciencias de la Salud; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Kaufman, Tomás. Universidad Maimonides. Facultad de Ciencias de la Salud; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vitullo, Alfredo Daniel. Universidad Maimonides. Facultad de Ciencias de la Salud; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Aptamers
Histochemistry
Mltiple sclerosis
Myelin basic protein
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/36348
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/36348
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Myelin Basic Protein and a Multiple Sclerosis-related MBP-peptide Bind to OligonucleotidesRozenblum, Tomas GuidoKaufman, TomásVitullo, Alfredo DanielAptamersHistochemistryMltiple sclerosisMyelin basic proteinhttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Aptamer ligands for myelin basic protein (MBP) were obtained using the systematic evolution of ligand by exponential enrichment (SELEX) method. Two clones were isolated from a pool of oligonucleotides and tested for MBP targeting. Using purified MBP, we demonstrated the binding activity of the aptamers and we also showed the affinity of MBP for oligonucleotides of specific length. Moreover, one selected aptamer competitively inhibited the binding of an MBP-specific antibody to MBP and the aptamer was found more sensitive than a commercial antibody. In addition, we showed the ability of the aptamer to detect myelin-rich regions in paraffin-embedded mouse brain tissue. Therefore, the MBP-binding activity of the selected oligonucleotide may prove useful as a tool for life science and medical research for myelin detection and might be a good lead for testing it in autoimmune diseases such as multiple sclerosis.Fil: Rozenblum, Tomas Guido. Universidad Maimonides. Facultad de Ciencias de la Salud; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Kaufman, Tomás. Universidad Maimonides. Facultad de Ciencias de la Salud; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Vitullo, Alfredo Daniel. Universidad Maimonides. Facultad de Ciencias de la Salud; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaThe American Society of Gene & Cell Therapy2014-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/36348Rozenblum, Tomas Guido; Kaufman, Tomás; Vitullo, Alfredo Daniel; Myelin Basic Protein and a Multiple Sclerosis-related MBP-peptide Bind to Oligonucleotides; The American Society of Gene & Cell Therapy; Molecular Therapy-Nucleic Acids; 3; 1-2014; 1-9; e1922162-2531CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1038/mtna.2014.43info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2162253116303316info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:39:30Zoai:ri.conicet.gov.ar:11336/36348instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:39:30.951CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Myelin Basic Protein and a Multiple Sclerosis-related MBP-peptide Bind to Oligonucleotides
title Myelin Basic Protein and a Multiple Sclerosis-related MBP-peptide Bind to Oligonucleotides
spellingShingle Myelin Basic Protein and a Multiple Sclerosis-related MBP-peptide Bind to Oligonucleotides
Rozenblum, Tomas Guido
Aptamers
Histochemistry
Mltiple sclerosis
Myelin basic protein
title_short Myelin Basic Protein and a Multiple Sclerosis-related MBP-peptide Bind to Oligonucleotides
title_full Myelin Basic Protein and a Multiple Sclerosis-related MBP-peptide Bind to Oligonucleotides
title_fullStr Myelin Basic Protein and a Multiple Sclerosis-related MBP-peptide Bind to Oligonucleotides
title_full_unstemmed Myelin Basic Protein and a Multiple Sclerosis-related MBP-peptide Bind to Oligonucleotides
title_sort Myelin Basic Protein and a Multiple Sclerosis-related MBP-peptide Bind to Oligonucleotides
dc.creator.none.fl_str_mv Rozenblum, Tomas Guido
Kaufman, Tomás
Vitullo, Alfredo Daniel
author Rozenblum, Tomas Guido
author_facet Rozenblum, Tomas Guido
Kaufman, Tomás
Vitullo, Alfredo Daniel
author_role author
author2 Kaufman, Tomás
Vitullo, Alfredo Daniel
author2_role author
author
dc.subject.none.fl_str_mv Aptamers
Histochemistry
Mltiple sclerosis
Myelin basic protein
topic Aptamers
Histochemistry
Mltiple sclerosis
Myelin basic protein
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Aptamer ligands for myelin basic protein (MBP) were obtained using the systematic evolution of ligand by exponential enrichment (SELEX) method. Two clones were isolated from a pool of oligonucleotides and tested for MBP targeting. Using purified MBP, we demonstrated the binding activity of the aptamers and we also showed the affinity of MBP for oligonucleotides of specific length. Moreover, one selected aptamer competitively inhibited the binding of an MBP-specific antibody to MBP and the aptamer was found more sensitive than a commercial antibody. In addition, we showed the ability of the aptamer to detect myelin-rich regions in paraffin-embedded mouse brain tissue. Therefore, the MBP-binding activity of the selected oligonucleotide may prove useful as a tool for life science and medical research for myelin detection and might be a good lead for testing it in autoimmune diseases such as multiple sclerosis.
Fil: Rozenblum, Tomas Guido. Universidad Maimonides. Facultad de Ciencias de la Salud; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Kaufman, Tomás. Universidad Maimonides. Facultad de Ciencias de la Salud; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vitullo, Alfredo Daniel. Universidad Maimonides. Facultad de Ciencias de la Salud; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Aptamer ligands for myelin basic protein (MBP) were obtained using the systematic evolution of ligand by exponential enrichment (SELEX) method. Two clones were isolated from a pool of oligonucleotides and tested for MBP targeting. Using purified MBP, we demonstrated the binding activity of the aptamers and we also showed the affinity of MBP for oligonucleotides of specific length. Moreover, one selected aptamer competitively inhibited the binding of an MBP-specific antibody to MBP and the aptamer was found more sensitive than a commercial antibody. In addition, we showed the ability of the aptamer to detect myelin-rich regions in paraffin-embedded mouse brain tissue. Therefore, the MBP-binding activity of the selected oligonucleotide may prove useful as a tool for life science and medical research for myelin detection and might be a good lead for testing it in autoimmune diseases such as multiple sclerosis.
publishDate 2014
dc.date.none.fl_str_mv 2014-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/36348
Rozenblum, Tomas Guido; Kaufman, Tomás; Vitullo, Alfredo Daniel; Myelin Basic Protein and a Multiple Sclerosis-related MBP-peptide Bind to Oligonucleotides; The American Society of Gene & Cell Therapy; Molecular Therapy-Nucleic Acids; 3; 1-2014; 1-9; e192
2162-2531
CONICET Digital
CONICET
url http://hdl.handle.net/11336/36348
identifier_str_mv Rozenblum, Tomas Guido; Kaufman, Tomás; Vitullo, Alfredo Daniel; Myelin Basic Protein and a Multiple Sclerosis-related MBP-peptide Bind to Oligonucleotides; The American Society of Gene & Cell Therapy; Molecular Therapy-Nucleic Acids; 3; 1-2014; 1-9; e192
2162-2531
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1038/mtna.2014.43
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2162253116303316
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv The American Society of Gene & Cell Therapy
publisher.none.fl_str_mv The American Society of Gene & Cell Therapy
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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