Lung oxidative metabolism after exposure to ambient particles

Autores
Magnani, Natalia Daniela; Marchini, Timoteo Oscar; Tasat, Deborah Ruth; Alvarez, Silvia; Evelson, Pablo Andrés
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this work was to study the time course of the oxidative metabolism in mice lung after exposure to ambient particles (ROFA). Swiss mice were intranasally instilled with a ROFA suspension (0.20. mg/kg). Animals were sacrificed 1 or 3. h after the exposure. Eighty percentage of increased oxygen consumption was observed in tissue cubes after 1. h of exposure. This observation was accompanied by an increased NADPH oxidase activity (40%) and mitochondrial oxygen consumption in state 3 (19%). NO production by lung homogenates was found to be increased by 43% after 3. h of exposure. Phospholipid oxidation in lung homogenates showed a 29% increase after 1. h of exposure, while a 30% increase in the carbonyl content was found only after 3. h of exposure. Our data show the relative importance of different sources of reactive oxygen species (NADPH oxidase activity and mitochondrial respiration) to the increased tissue oxygen consumption, oxidative damage and antioxidant status observed in an acute model of ROFA particles exposure.
Fil: Magnani, Natalia Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina
Fil: Marchini, Timoteo Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina
Fil: Tasat, Deborah Ruth. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología. Centro de Estudios en Salud y Medio Ambiente; Argentina
Fil: Alvarez, Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina
Fil: Evelson, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina
Materia
Air Particle Pollutants
Lung
Oxygen Consumption
Reactive Oxygen Species
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/67463
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/67463
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Lung oxidative metabolism after exposure to ambient particlesMagnani, Natalia DanielaMarchini, Timoteo OscarTasat, Deborah RuthAlvarez, SilviaEvelson, Pablo AndrésAir Particle PollutantsLungOxygen ConsumptionReactive Oxygen Specieshttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3The aim of this work was to study the time course of the oxidative metabolism in mice lung after exposure to ambient particles (ROFA). Swiss mice were intranasally instilled with a ROFA suspension (0.20. mg/kg). Animals were sacrificed 1 or 3. h after the exposure. Eighty percentage of increased oxygen consumption was observed in tissue cubes after 1. h of exposure. This observation was accompanied by an increased NADPH oxidase activity (40%) and mitochondrial oxygen consumption in state 3 (19%). NO production by lung homogenates was found to be increased by 43% after 3. h of exposure. Phospholipid oxidation in lung homogenates showed a 29% increase after 1. h of exposure, while a 30% increase in the carbonyl content was found only after 3. h of exposure. Our data show the relative importance of different sources of reactive oxygen species (NADPH oxidase activity and mitochondrial respiration) to the increased tissue oxygen consumption, oxidative damage and antioxidant status observed in an acute model of ROFA particles exposure.Fil: Magnani, Natalia Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; ArgentinaFil: Marchini, Timoteo Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; ArgentinaFil: Tasat, Deborah Ruth. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología. Centro de Estudios en Salud y Medio Ambiente; ArgentinaFil: Alvarez, Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; ArgentinaFil: Evelson, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; ArgentinaAcademic Press Inc Elsevier Science2011-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/67463Magnani, Natalia Daniela; Marchini, Timoteo Oscar; Tasat, Deborah Ruth; Alvarez, Silvia; Evelson, Pablo Andrés; Lung oxidative metabolism after exposure to ambient particles; Academic Press Inc Elsevier Science; Biochemical and Biophysical Research Communications; 412; 4; 9-2011; 667-6720006-291XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbrc.2011.08.021info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0006291X11014069info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:19:11Zoai:ri.conicet.gov.ar:11336/67463instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:19:12.172CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Lung oxidative metabolism after exposure to ambient particles
title Lung oxidative metabolism after exposure to ambient particles
spellingShingle Lung oxidative metabolism after exposure to ambient particles
Magnani, Natalia Daniela
Air Particle Pollutants
Lung
Oxygen Consumption
Reactive Oxygen Species
title_short Lung oxidative metabolism after exposure to ambient particles
title_full Lung oxidative metabolism after exposure to ambient particles
title_fullStr Lung oxidative metabolism after exposure to ambient particles
title_full_unstemmed Lung oxidative metabolism after exposure to ambient particles
title_sort Lung oxidative metabolism after exposure to ambient particles
dc.creator.none.fl_str_mv Magnani, Natalia Daniela
Marchini, Timoteo Oscar
Tasat, Deborah Ruth
Alvarez, Silvia
Evelson, Pablo Andrés
author Magnani, Natalia Daniela
author_facet Magnani, Natalia Daniela
Marchini, Timoteo Oscar
Tasat, Deborah Ruth
Alvarez, Silvia
Evelson, Pablo Andrés
author_role author
author2 Marchini, Timoteo Oscar
Tasat, Deborah Ruth
Alvarez, Silvia
Evelson, Pablo Andrés
author2_role author
author
author
author
dc.subject.none.fl_str_mv Air Particle Pollutants
Lung
Oxygen Consumption
Reactive Oxygen Species
topic Air Particle Pollutants
Lung
Oxygen Consumption
Reactive Oxygen Species
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The aim of this work was to study the time course of the oxidative metabolism in mice lung after exposure to ambient particles (ROFA). Swiss mice were intranasally instilled with a ROFA suspension (0.20. mg/kg). Animals were sacrificed 1 or 3. h after the exposure. Eighty percentage of increased oxygen consumption was observed in tissue cubes after 1. h of exposure. This observation was accompanied by an increased NADPH oxidase activity (40%) and mitochondrial oxygen consumption in state 3 (19%). NO production by lung homogenates was found to be increased by 43% after 3. h of exposure. Phospholipid oxidation in lung homogenates showed a 29% increase after 1. h of exposure, while a 30% increase in the carbonyl content was found only after 3. h of exposure. Our data show the relative importance of different sources of reactive oxygen species (NADPH oxidase activity and mitochondrial respiration) to the increased tissue oxygen consumption, oxidative damage and antioxidant status observed in an acute model of ROFA particles exposure.
Fil: Magnani, Natalia Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina
Fil: Marchini, Timoteo Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina
Fil: Tasat, Deborah Ruth. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología. Centro de Estudios en Salud y Medio Ambiente; Argentina
Fil: Alvarez, Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina
Fil: Evelson, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina
description The aim of this work was to study the time course of the oxidative metabolism in mice lung after exposure to ambient particles (ROFA). Swiss mice were intranasally instilled with a ROFA suspension (0.20. mg/kg). Animals were sacrificed 1 or 3. h after the exposure. Eighty percentage of increased oxygen consumption was observed in tissue cubes after 1. h of exposure. This observation was accompanied by an increased NADPH oxidase activity (40%) and mitochondrial oxygen consumption in state 3 (19%). NO production by lung homogenates was found to be increased by 43% after 3. h of exposure. Phospholipid oxidation in lung homogenates showed a 29% increase after 1. h of exposure, while a 30% increase in the carbonyl content was found only after 3. h of exposure. Our data show the relative importance of different sources of reactive oxygen species (NADPH oxidase activity and mitochondrial respiration) to the increased tissue oxygen consumption, oxidative damage and antioxidant status observed in an acute model of ROFA particles exposure.
publishDate 2011
dc.date.none.fl_str_mv 2011-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/67463
Magnani, Natalia Daniela; Marchini, Timoteo Oscar; Tasat, Deborah Ruth; Alvarez, Silvia; Evelson, Pablo Andrés; Lung oxidative metabolism after exposure to ambient particles; Academic Press Inc Elsevier Science; Biochemical and Biophysical Research Communications; 412; 4; 9-2011; 667-672
0006-291X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/67463
identifier_str_mv Magnani, Natalia Daniela; Marchini, Timoteo Oscar; Tasat, Deborah Ruth; Alvarez, Silvia; Evelson, Pablo Andrés; Lung oxidative metabolism after exposure to ambient particles; Academic Press Inc Elsevier Science; Biochemical and Biophysical Research Communications; 412; 4; 9-2011; 667-672
0006-291X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbrc.2011.08.021
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0006291X11014069
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842981045309276160
score 12.48226

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