Elemental composition of strawberry plants inoculated with the plant growth-promoting bacterium Azospirillum brasilense REC3 assessed by scanning electron microscopy and energy dis...

Autores
Guerrero Molina, María Fernanda; Lovaisa, Nadia Carolina; Salazar, Sergio Miguel; Diaz Ricci, Juan Carlos; Pedraza, Raúl Osvaldo
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The elemental composition of strawberry plants (Fragaria ananassa cv. Macarena) inoculated with the plant growth-promoting bacterium Azospirillum brasilense REC3 and non-inoculated controls, was studied for the first time by using scanning electron microscopy (SEM) and energy dispersive X-ray (EDS) analysis. This technique allowed semi-quantification of different elements simultaneously using a small solid sample. Plants were inoculated and grown hydroponically in 50 % and 100 % Hoagland solution, corresponding to a poor- or rich- nutrient medium, respectively. Bacterial inoculated plants increased 45% and 80 % strawberry growth index compared to their controls when grown in 100% and 50% Hoagland solution, respectively. Thus, inoculation with A. brasilense REC3 in a poor-nutrient medium had the largest effect in terms of increasing both shoot and root biomass as well as growth index. SEM-EDS spectra and maps showed the elemental composition and relative distribution of nutrients in strawberry tissues. Leaves were composed of C, O, N, Na, P, K, Ca and Cu, while roots had also Si and Cl. The organic fraction (C, O and N) accounted for over the 96.3 % of the total chemical composition, and among the mineral fraction composition, Na exhibited a higher accumulation in both tissues. Azospirillum-inoculated and control plants showed similar elemental quantification values. Although, in bacteria-inoculated roots, P was significantly increased (34.33 %) while Cu was decreased (35.16 %).
Fil: Guerrero Molina, María Fernanda. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Lovaisa, Nadia Carolina. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Salazar, Sergio Miguel. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina
Fil: Diaz Ricci, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Pedraza, Raúl Osvaldo. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina
Materia
Azospirillum Brasilense
Energy Dispersive X-Ray Spectroscopy
Plant Mineral Nutrition
Scanning Electron Microscopy
Fragaria Ananassa
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/7222
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/7222
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Elemental composition of strawberry plants inoculated with the plant growth-promoting bacterium Azospirillum brasilense REC3 assessed by scanning electron microscopy and energy dispersive X-ray analysisGuerrero Molina, María FernandaLovaisa, Nadia CarolinaSalazar, Sergio MiguelDiaz Ricci, Juan CarlosPedraza, Raúl OsvaldoAzospirillum BrasilenseEnergy Dispersive X-Ray SpectroscopyPlant Mineral NutritionScanning Electron MicroscopyFragaria Ananassahttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4The elemental composition of strawberry plants (Fragaria ananassa cv. Macarena) inoculated with the plant growth-promoting bacterium Azospirillum brasilense REC3 and non-inoculated controls, was studied for the first time by using scanning electron microscopy (SEM) and energy dispersive X-ray (EDS) analysis. This technique allowed semi-quantification of different elements simultaneously using a small solid sample. Plants were inoculated and grown hydroponically in 50 % and 100 % Hoagland solution, corresponding to a poor- or rich- nutrient medium, respectively. Bacterial inoculated plants increased 45% and 80 % strawberry growth index compared to their controls when grown in 100% and 50% Hoagland solution, respectively. Thus, inoculation with A. brasilense REC3 in a poor-nutrient medium had the largest effect in terms of increasing both shoot and root biomass as well as growth index. SEM-EDS spectra and maps showed the elemental composition and relative distribution of nutrients in strawberry tissues. Leaves were composed of C, O, N, Na, P, K, Ca and Cu, while roots had also Si and Cl. The organic fraction (C, O and N) accounted for over the 96.3 % of the total chemical composition, and among the mineral fraction composition, Na exhibited a higher accumulation in both tissues. Azospirillum-inoculated and control plants showed similar elemental quantification values. Although, in bacteria-inoculated roots, P was significantly increased (34.33 %) while Cu was decreased (35.16 %).Fil: Guerrero Molina, María Fernanda. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Lovaisa, Nadia Carolina. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Salazar, Sergio Miguel. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; ArgentinaFil: Diaz Ricci, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Pedraza, Raúl Osvaldo. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; ArgentinaWiley2013-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/7222Guerrero Molina, María Fernanda; Lovaisa, Nadia Carolina; Salazar, Sergio Miguel; Diaz Ricci, Juan Carlos; Pedraza, Raúl Osvaldo; Elemental composition of strawberry plants inoculated with the plant growth-promoting bacterium Azospirillum brasilense REC3 assessed by scanning electron microscopy and energy dispersive X-ray analysis; Wiley; Plant Biology; 16; 4; 7-2013; 726-7311435-8603enginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/plb.12114/abstractinfo:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/doi/10.1111/plb.12114info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:22:01Zoai:ri.conicet.gov.ar:11336/7222instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:22:01.8CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Elemental composition of strawberry plants inoculated with the plant growth-promoting bacterium Azospirillum brasilense REC3 assessed by scanning electron microscopy and energy dispersive X-ray analysis
title Elemental composition of strawberry plants inoculated with the plant growth-promoting bacterium Azospirillum brasilense REC3 assessed by scanning electron microscopy and energy dispersive X-ray analysis
spellingShingle Elemental composition of strawberry plants inoculated with the plant growth-promoting bacterium Azospirillum brasilense REC3 assessed by scanning electron microscopy and energy dispersive X-ray analysis
Guerrero Molina, María Fernanda
Azospirillum Brasilense
Energy Dispersive X-Ray Spectroscopy
Plant Mineral Nutrition
Scanning Electron Microscopy
Fragaria Ananassa
title_short Elemental composition of strawberry plants inoculated with the plant growth-promoting bacterium Azospirillum brasilense REC3 assessed by scanning electron microscopy and energy dispersive X-ray analysis
title_full Elemental composition of strawberry plants inoculated with the plant growth-promoting bacterium Azospirillum brasilense REC3 assessed by scanning electron microscopy and energy dispersive X-ray analysis
title_fullStr Elemental composition of strawberry plants inoculated with the plant growth-promoting bacterium Azospirillum brasilense REC3 assessed by scanning electron microscopy and energy dispersive X-ray analysis
title_full_unstemmed Elemental composition of strawberry plants inoculated with the plant growth-promoting bacterium Azospirillum brasilense REC3 assessed by scanning electron microscopy and energy dispersive X-ray analysis
title_sort Elemental composition of strawberry plants inoculated with the plant growth-promoting bacterium Azospirillum brasilense REC3 assessed by scanning electron microscopy and energy dispersive X-ray analysis
dc.creator.none.fl_str_mv Guerrero Molina, María Fernanda
Lovaisa, Nadia Carolina
Salazar, Sergio Miguel
Diaz Ricci, Juan Carlos
Pedraza, Raúl Osvaldo
author Guerrero Molina, María Fernanda
author_facet Guerrero Molina, María Fernanda
Lovaisa, Nadia Carolina
Salazar, Sergio Miguel
Diaz Ricci, Juan Carlos
Pedraza, Raúl Osvaldo
author_role author
author2 Lovaisa, Nadia Carolina
Salazar, Sergio Miguel
Diaz Ricci, Juan Carlos
Pedraza, Raúl Osvaldo
author2_role author
author
author
author
dc.subject.none.fl_str_mv Azospirillum Brasilense
Energy Dispersive X-Ray Spectroscopy
Plant Mineral Nutrition
Scanning Electron Microscopy
Fragaria Ananassa
topic Azospirillum Brasilense
Energy Dispersive X-Ray Spectroscopy
Plant Mineral Nutrition
Scanning Electron Microscopy
Fragaria Ananassa
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The elemental composition of strawberry plants (Fragaria ananassa cv. Macarena) inoculated with the plant growth-promoting bacterium Azospirillum brasilense REC3 and non-inoculated controls, was studied for the first time by using scanning electron microscopy (SEM) and energy dispersive X-ray (EDS) analysis. This technique allowed semi-quantification of different elements simultaneously using a small solid sample. Plants were inoculated and grown hydroponically in 50 % and 100 % Hoagland solution, corresponding to a poor- or rich- nutrient medium, respectively. Bacterial inoculated plants increased 45% and 80 % strawberry growth index compared to their controls when grown in 100% and 50% Hoagland solution, respectively. Thus, inoculation with A. brasilense REC3 in a poor-nutrient medium had the largest effect in terms of increasing both shoot and root biomass as well as growth index. SEM-EDS spectra and maps showed the elemental composition and relative distribution of nutrients in strawberry tissues. Leaves were composed of C, O, N, Na, P, K, Ca and Cu, while roots had also Si and Cl. The organic fraction (C, O and N) accounted for over the 96.3 % of the total chemical composition, and among the mineral fraction composition, Na exhibited a higher accumulation in both tissues. Azospirillum-inoculated and control plants showed similar elemental quantification values. Although, in bacteria-inoculated roots, P was significantly increased (34.33 %) while Cu was decreased (35.16 %).
Fil: Guerrero Molina, María Fernanda. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Lovaisa, Nadia Carolina. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Salazar, Sergio Miguel. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina
Fil: Diaz Ricci, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Pedraza, Raúl Osvaldo. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina
description The elemental composition of strawberry plants (Fragaria ananassa cv. Macarena) inoculated with the plant growth-promoting bacterium Azospirillum brasilense REC3 and non-inoculated controls, was studied for the first time by using scanning electron microscopy (SEM) and energy dispersive X-ray (EDS) analysis. This technique allowed semi-quantification of different elements simultaneously using a small solid sample. Plants were inoculated and grown hydroponically in 50 % and 100 % Hoagland solution, corresponding to a poor- or rich- nutrient medium, respectively. Bacterial inoculated plants increased 45% and 80 % strawberry growth index compared to their controls when grown in 100% and 50% Hoagland solution, respectively. Thus, inoculation with A. brasilense REC3 in a poor-nutrient medium had the largest effect in terms of increasing both shoot and root biomass as well as growth index. SEM-EDS spectra and maps showed the elemental composition and relative distribution of nutrients in strawberry tissues. Leaves were composed of C, O, N, Na, P, K, Ca and Cu, while roots had also Si and Cl. The organic fraction (C, O and N) accounted for over the 96.3 % of the total chemical composition, and among the mineral fraction composition, Na exhibited a higher accumulation in both tissues. Azospirillum-inoculated and control plants showed similar elemental quantification values. Although, in bacteria-inoculated roots, P was significantly increased (34.33 %) while Cu was decreased (35.16 %).
publishDate 2013
dc.date.none.fl_str_mv 2013-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/7222
Guerrero Molina, María Fernanda; Lovaisa, Nadia Carolina; Salazar, Sergio Miguel; Diaz Ricci, Juan Carlos; Pedraza, Raúl Osvaldo; Elemental composition of strawberry plants inoculated with the plant growth-promoting bacterium Azospirillum brasilense REC3 assessed by scanning electron microscopy and energy dispersive X-ray analysis; Wiley; Plant Biology; 16; 4; 7-2013; 726-731
1435-8603
url http://hdl.handle.net/11336/7222
identifier_str_mv Guerrero Molina, María Fernanda; Lovaisa, Nadia Carolina; Salazar, Sergio Miguel; Diaz Ricci, Juan Carlos; Pedraza, Raúl Osvaldo; Elemental composition of strawberry plants inoculated with the plant growth-promoting bacterium Azospirillum brasilense REC3 assessed by scanning electron microscopy and energy dispersive X-ray analysis; Wiley; Plant Biology; 16; 4; 7-2013; 726-731
1435-8603
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/plb.12114/abstract
info:eu-repo/semantics/altIdentifier/doi/
info:eu-repo/semantics/altIdentifier/doi/10.1111/plb.12114
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.48226

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