More than rhizosphere colonization of strawberry plants by Azospirillum brasilense

Autores
Guerrero Molina, María Fernanda; Winik, Beatriz Clara; Pedraza, Raúl Osvaldo
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Azospirillum brasilense is a plant growth promoting bacteria (PGPB), capable of improving growth and yield of important crops including strawberry (Fragaria ananassa, Duch.). Considering that for commercial purposes strawberry plants are asexually reproduced by planting stolons into the soil, the aim of this work was to evaluate Azospirillum root association and further colonization through stolons from bacterial inoculated strawberry mother-plants to new born daughter-plants. For that, three commercial cultivars of strawberry ('Camarosa', 'Milsei' and 'Selva') were root inoculated with two strains of A. brasilense: REC3 and PEC5. Scanning and transmission electron microscopy observations showed clear bacterial attachment to the root surface and colonization of root and stolon inner tissues. The diazotrophic bacteria were re-isolated from inoculated mother-plants, non-inoculated daughters and stolons using N-free NFb semisolid medium. In all cases, the MPN from root samples was higher than from stolons. The bacterial nifD gene, essential in the biological N 2-fixing process, was PCR-amplified from DNA of roots and stolons proving the occurrence of diazotrophs within these tissues. To confirm that these bacteria corresponded to the inoculated PGPB, the 16S rDNA gene of re-isolates was subjected to amplified rDNA restriction analysis (ARDRA) and to automated DNA sequencing, revealing that they belong to Azospirillum brasilense. This confirms effective rhizosphere colonization of strawberry mother-plants and also the colonization of A. brasilense to new daughter-plants via stolons. This is the first report about A. brasilense colonization from one strawberry plant to another one by colonizing inner tissues of roots and stolons. This means that a single inoculation with selected PGPB would allow the growers to have numerous plant generations at nursery already inoculated and with better conditions to be planted at field, contributing to a sustainable agricultural practice.
Fil: Guerrero Molina, María Fernanda. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Winik, Beatriz Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Pedraza, Raúl Osvaldo. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina
Materia
AZOSPIRILLUM BRASILENSE
ELECTRON MICROSCOPY
FRAGARIA ANANASSA
NIFD
PGPB
STOLON
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/195604

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oai_identifier_str oai:ri.conicet.gov.ar:11336/195604
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network_name_str CONICET Digital (CONICET)
spelling More than rhizosphere colonization of strawberry plants by Azospirillum brasilenseGuerrero Molina, María FernandaWinik, Beatriz ClaraPedraza, Raúl OsvaldoAZOSPIRILLUM BRASILENSEELECTRON MICROSCOPYFRAGARIA ANANASSANIFDPGPBSTOLONhttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4Azospirillum brasilense is a plant growth promoting bacteria (PGPB), capable of improving growth and yield of important crops including strawberry (Fragaria ananassa, Duch.). Considering that for commercial purposes strawberry plants are asexually reproduced by planting stolons into the soil, the aim of this work was to evaluate Azospirillum root association and further colonization through stolons from bacterial inoculated strawberry mother-plants to new born daughter-plants. For that, three commercial cultivars of strawberry ('Camarosa', 'Milsei' and 'Selva') were root inoculated with two strains of A. brasilense: REC3 and PEC5. Scanning and transmission electron microscopy observations showed clear bacterial attachment to the root surface and colonization of root and stolon inner tissues. The diazotrophic bacteria were re-isolated from inoculated mother-plants, non-inoculated daughters and stolons using N-free NFb semisolid medium. In all cases, the MPN from root samples was higher than from stolons. The bacterial nifD gene, essential in the biological N 2-fixing process, was PCR-amplified from DNA of roots and stolons proving the occurrence of diazotrophs within these tissues. To confirm that these bacteria corresponded to the inoculated PGPB, the 16S rDNA gene of re-isolates was subjected to amplified rDNA restriction analysis (ARDRA) and to automated DNA sequencing, revealing that they belong to Azospirillum brasilense. This confirms effective rhizosphere colonization of strawberry mother-plants and also the colonization of A. brasilense to new daughter-plants via stolons. This is the first report about A. brasilense colonization from one strawberry plant to another one by colonizing inner tissues of roots and stolons. This means that a single inoculation with selected PGPB would allow the growers to have numerous plant generations at nursery already inoculated and with better conditions to be planted at field, contributing to a sustainable agricultural practice.Fil: Guerrero Molina, María Fernanda. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Winik, Beatriz Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Pedraza, Raúl Osvaldo. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; ArgentinaElsevier Science2012-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/195604Guerrero Molina, María Fernanda; Winik, Beatriz Clara; Pedraza, Raúl Osvaldo; More than rhizosphere colonization of strawberry plants by Azospirillum brasilense; Elsevier Science; Applied Soil Ecology; 61; 6-2012; 205-2120929-1393CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.apsoil.2011.10.011info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0929139311002307info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:01:39Zoai:ri.conicet.gov.ar:11336/195604instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:01:39.787CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv More than rhizosphere colonization of strawberry plants by Azospirillum brasilense
title More than rhizosphere colonization of strawberry plants by Azospirillum brasilense
spellingShingle More than rhizosphere colonization of strawberry plants by Azospirillum brasilense
Guerrero Molina, María Fernanda
AZOSPIRILLUM BRASILENSE
ELECTRON MICROSCOPY
FRAGARIA ANANASSA
NIFD
PGPB
STOLON
title_short More than rhizosphere colonization of strawberry plants by Azospirillum brasilense
title_full More than rhizosphere colonization of strawberry plants by Azospirillum brasilense
title_fullStr More than rhizosphere colonization of strawberry plants by Azospirillum brasilense
title_full_unstemmed More than rhizosphere colonization of strawberry plants by Azospirillum brasilense
title_sort More than rhizosphere colonization of strawberry plants by Azospirillum brasilense
dc.creator.none.fl_str_mv Guerrero Molina, María Fernanda
Winik, Beatriz Clara
Pedraza, Raúl Osvaldo
author Guerrero Molina, María Fernanda
author_facet Guerrero Molina, María Fernanda
Winik, Beatriz Clara
Pedraza, Raúl Osvaldo
author_role author
author2 Winik, Beatriz Clara
Pedraza, Raúl Osvaldo
author2_role author
author
dc.subject.none.fl_str_mv AZOSPIRILLUM BRASILENSE
ELECTRON MICROSCOPY
FRAGARIA ANANASSA
NIFD
PGPB
STOLON
topic AZOSPIRILLUM BRASILENSE
ELECTRON MICROSCOPY
FRAGARIA ANANASSA
NIFD
PGPB
STOLON
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Azospirillum brasilense is a plant growth promoting bacteria (PGPB), capable of improving growth and yield of important crops including strawberry (Fragaria ananassa, Duch.). Considering that for commercial purposes strawberry plants are asexually reproduced by planting stolons into the soil, the aim of this work was to evaluate Azospirillum root association and further colonization through stolons from bacterial inoculated strawberry mother-plants to new born daughter-plants. For that, three commercial cultivars of strawberry ('Camarosa', 'Milsei' and 'Selva') were root inoculated with two strains of A. brasilense: REC3 and PEC5. Scanning and transmission electron microscopy observations showed clear bacterial attachment to the root surface and colonization of root and stolon inner tissues. The diazotrophic bacteria were re-isolated from inoculated mother-plants, non-inoculated daughters and stolons using N-free NFb semisolid medium. In all cases, the MPN from root samples was higher than from stolons. The bacterial nifD gene, essential in the biological N 2-fixing process, was PCR-amplified from DNA of roots and stolons proving the occurrence of diazotrophs within these tissues. To confirm that these bacteria corresponded to the inoculated PGPB, the 16S rDNA gene of re-isolates was subjected to amplified rDNA restriction analysis (ARDRA) and to automated DNA sequencing, revealing that they belong to Azospirillum brasilense. This confirms effective rhizosphere colonization of strawberry mother-plants and also the colonization of A. brasilense to new daughter-plants via stolons. This is the first report about A. brasilense colonization from one strawberry plant to another one by colonizing inner tissues of roots and stolons. This means that a single inoculation with selected PGPB would allow the growers to have numerous plant generations at nursery already inoculated and with better conditions to be planted at field, contributing to a sustainable agricultural practice.
Fil: Guerrero Molina, María Fernanda. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Winik, Beatriz Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Pedraza, Raúl Osvaldo. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina
description Azospirillum brasilense is a plant growth promoting bacteria (PGPB), capable of improving growth and yield of important crops including strawberry (Fragaria ananassa, Duch.). Considering that for commercial purposes strawberry plants are asexually reproduced by planting stolons into the soil, the aim of this work was to evaluate Azospirillum root association and further colonization through stolons from bacterial inoculated strawberry mother-plants to new born daughter-plants. For that, three commercial cultivars of strawberry ('Camarosa', 'Milsei' and 'Selva') were root inoculated with two strains of A. brasilense: REC3 and PEC5. Scanning and transmission electron microscopy observations showed clear bacterial attachment to the root surface and colonization of root and stolon inner tissues. The diazotrophic bacteria were re-isolated from inoculated mother-plants, non-inoculated daughters and stolons using N-free NFb semisolid medium. In all cases, the MPN from root samples was higher than from stolons. The bacterial nifD gene, essential in the biological N 2-fixing process, was PCR-amplified from DNA of roots and stolons proving the occurrence of diazotrophs within these tissues. To confirm that these bacteria corresponded to the inoculated PGPB, the 16S rDNA gene of re-isolates was subjected to amplified rDNA restriction analysis (ARDRA) and to automated DNA sequencing, revealing that they belong to Azospirillum brasilense. This confirms effective rhizosphere colonization of strawberry mother-plants and also the colonization of A. brasilense to new daughter-plants via stolons. This is the first report about A. brasilense colonization from one strawberry plant to another one by colonizing inner tissues of roots and stolons. This means that a single inoculation with selected PGPB would allow the growers to have numerous plant generations at nursery already inoculated and with better conditions to be planted at field, contributing to a sustainable agricultural practice.
publishDate 2012
dc.date.none.fl_str_mv 2012-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/195604
Guerrero Molina, María Fernanda; Winik, Beatriz Clara; Pedraza, Raúl Osvaldo; More than rhizosphere colonization of strawberry plants by Azospirillum brasilense; Elsevier Science; Applied Soil Ecology; 61; 6-2012; 205-212
0929-1393
CONICET Digital
CONICET
url http://hdl.handle.net/11336/195604
identifier_str_mv Guerrero Molina, María Fernanda; Winik, Beatriz Clara; Pedraza, Raúl Osvaldo; More than rhizosphere colonization of strawberry plants by Azospirillum brasilense; Elsevier Science; Applied Soil Ecology; 61; 6-2012; 205-212
0929-1393
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.apsoil.2011.10.011
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0929139311002307
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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