More than rhizosphere colonization of strawberry plants by Azospirillum brasilense
- Autores
- Guerrero Molina, María Fernanda; Winik, Beatriz Clara; Pedraza, Raúl Osvaldo
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Azospirillum brasilense is a plant growth promoting bacteria (PGPB), capable of improving growth and yield of important crops including strawberry (Fragaria ananassa, Duch.). Considering that for commercial purposes strawberry plants are asexually reproduced by planting stolons into the soil, the aim of this work was to evaluate Azospirillum root association and further colonization through stolons from bacterial inoculated strawberry mother-plants to new born daughter-plants. For that, three commercial cultivars of strawberry ('Camarosa', 'Milsei' and 'Selva') were root inoculated with two strains of A. brasilense: REC3 and PEC5. Scanning and transmission electron microscopy observations showed clear bacterial attachment to the root surface and colonization of root and stolon inner tissues. The diazotrophic bacteria were re-isolated from inoculated mother-plants, non-inoculated daughters and stolons using N-free NFb semisolid medium. In all cases, the MPN from root samples was higher than from stolons. The bacterial nifD gene, essential in the biological N 2-fixing process, was PCR-amplified from DNA of roots and stolons proving the occurrence of diazotrophs within these tissues. To confirm that these bacteria corresponded to the inoculated PGPB, the 16S rDNA gene of re-isolates was subjected to amplified rDNA restriction analysis (ARDRA) and to automated DNA sequencing, revealing that they belong to Azospirillum brasilense. This confirms effective rhizosphere colonization of strawberry mother-plants and also the colonization of A. brasilense to new daughter-plants via stolons. This is the first report about A. brasilense colonization from one strawberry plant to another one by colonizing inner tissues of roots and stolons. This means that a single inoculation with selected PGPB would allow the growers to have numerous plant generations at nursery already inoculated and with better conditions to be planted at field, contributing to a sustainable agricultural practice.
Fil: Guerrero Molina, María Fernanda. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Winik, Beatriz Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Pedraza, Raúl Osvaldo. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina - Materia
-
AZOSPIRILLUM BRASILENSE
ELECTRON MICROSCOPY
FRAGARIA ANANASSA
NIFD
PGPB
STOLON - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/195604
Ver los metadatos del registro completo
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network_name_str |
CONICET Digital (CONICET) |
spelling |
More than rhizosphere colonization of strawberry plants by Azospirillum brasilenseGuerrero Molina, María FernandaWinik, Beatriz ClaraPedraza, Raúl OsvaldoAZOSPIRILLUM BRASILENSEELECTRON MICROSCOPYFRAGARIA ANANASSANIFDPGPBSTOLONhttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4Azospirillum brasilense is a plant growth promoting bacteria (PGPB), capable of improving growth and yield of important crops including strawberry (Fragaria ananassa, Duch.). Considering that for commercial purposes strawberry plants are asexually reproduced by planting stolons into the soil, the aim of this work was to evaluate Azospirillum root association and further colonization through stolons from bacterial inoculated strawberry mother-plants to new born daughter-plants. For that, three commercial cultivars of strawberry ('Camarosa', 'Milsei' and 'Selva') were root inoculated with two strains of A. brasilense: REC3 and PEC5. Scanning and transmission electron microscopy observations showed clear bacterial attachment to the root surface and colonization of root and stolon inner tissues. The diazotrophic bacteria were re-isolated from inoculated mother-plants, non-inoculated daughters and stolons using N-free NFb semisolid medium. In all cases, the MPN from root samples was higher than from stolons. The bacterial nifD gene, essential in the biological N 2-fixing process, was PCR-amplified from DNA of roots and stolons proving the occurrence of diazotrophs within these tissues. To confirm that these bacteria corresponded to the inoculated PGPB, the 16S rDNA gene of re-isolates was subjected to amplified rDNA restriction analysis (ARDRA) and to automated DNA sequencing, revealing that they belong to Azospirillum brasilense. This confirms effective rhizosphere colonization of strawberry mother-plants and also the colonization of A. brasilense to new daughter-plants via stolons. This is the first report about A. brasilense colonization from one strawberry plant to another one by colonizing inner tissues of roots and stolons. This means that a single inoculation with selected PGPB would allow the growers to have numerous plant generations at nursery already inoculated and with better conditions to be planted at field, contributing to a sustainable agricultural practice.Fil: Guerrero Molina, María Fernanda. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Winik, Beatriz Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Pedraza, Raúl Osvaldo. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; ArgentinaElsevier Science2012-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/195604Guerrero Molina, María Fernanda; Winik, Beatriz Clara; Pedraza, Raúl Osvaldo; More than rhizosphere colonization of strawberry plants by Azospirillum brasilense; Elsevier Science; Applied Soil Ecology; 61; 6-2012; 205-2120929-1393CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.apsoil.2011.10.011info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0929139311002307info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:01:39Zoai:ri.conicet.gov.ar:11336/195604instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:01:39.787CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
More than rhizosphere colonization of strawberry plants by Azospirillum brasilense |
title |
More than rhizosphere colonization of strawberry plants by Azospirillum brasilense |
spellingShingle |
More than rhizosphere colonization of strawberry plants by Azospirillum brasilense Guerrero Molina, María Fernanda AZOSPIRILLUM BRASILENSE ELECTRON MICROSCOPY FRAGARIA ANANASSA NIFD PGPB STOLON |
title_short |
More than rhizosphere colonization of strawberry plants by Azospirillum brasilense |
title_full |
More than rhizosphere colonization of strawberry plants by Azospirillum brasilense |
title_fullStr |
More than rhizosphere colonization of strawberry plants by Azospirillum brasilense |
title_full_unstemmed |
More than rhizosphere colonization of strawberry plants by Azospirillum brasilense |
title_sort |
More than rhizosphere colonization of strawberry plants by Azospirillum brasilense |
dc.creator.none.fl_str_mv |
Guerrero Molina, María Fernanda Winik, Beatriz Clara Pedraza, Raúl Osvaldo |
author |
Guerrero Molina, María Fernanda |
author_facet |
Guerrero Molina, María Fernanda Winik, Beatriz Clara Pedraza, Raúl Osvaldo |
author_role |
author |
author2 |
Winik, Beatriz Clara Pedraza, Raúl Osvaldo |
author2_role |
author author |
dc.subject.none.fl_str_mv |
AZOSPIRILLUM BRASILENSE ELECTRON MICROSCOPY FRAGARIA ANANASSA NIFD PGPB STOLON |
topic |
AZOSPIRILLUM BRASILENSE ELECTRON MICROSCOPY FRAGARIA ANANASSA NIFD PGPB STOLON |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/4.1 https://purl.org/becyt/ford/4 |
dc.description.none.fl_txt_mv |
Azospirillum brasilense is a plant growth promoting bacteria (PGPB), capable of improving growth and yield of important crops including strawberry (Fragaria ananassa, Duch.). Considering that for commercial purposes strawberry plants are asexually reproduced by planting stolons into the soil, the aim of this work was to evaluate Azospirillum root association and further colonization through stolons from bacterial inoculated strawberry mother-plants to new born daughter-plants. For that, three commercial cultivars of strawberry ('Camarosa', 'Milsei' and 'Selva') were root inoculated with two strains of A. brasilense: REC3 and PEC5. Scanning and transmission electron microscopy observations showed clear bacterial attachment to the root surface and colonization of root and stolon inner tissues. The diazotrophic bacteria were re-isolated from inoculated mother-plants, non-inoculated daughters and stolons using N-free NFb semisolid medium. In all cases, the MPN from root samples was higher than from stolons. The bacterial nifD gene, essential in the biological N 2-fixing process, was PCR-amplified from DNA of roots and stolons proving the occurrence of diazotrophs within these tissues. To confirm that these bacteria corresponded to the inoculated PGPB, the 16S rDNA gene of re-isolates was subjected to amplified rDNA restriction analysis (ARDRA) and to automated DNA sequencing, revealing that they belong to Azospirillum brasilense. This confirms effective rhizosphere colonization of strawberry mother-plants and also the colonization of A. brasilense to new daughter-plants via stolons. This is the first report about A. brasilense colonization from one strawberry plant to another one by colonizing inner tissues of roots and stolons. This means that a single inoculation with selected PGPB would allow the growers to have numerous plant generations at nursery already inoculated and with better conditions to be planted at field, contributing to a sustainable agricultural practice. Fil: Guerrero Molina, María Fernanda. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Winik, Beatriz Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina Fil: Pedraza, Raúl Osvaldo. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina |
description |
Azospirillum brasilense is a plant growth promoting bacteria (PGPB), capable of improving growth and yield of important crops including strawberry (Fragaria ananassa, Duch.). Considering that for commercial purposes strawberry plants are asexually reproduced by planting stolons into the soil, the aim of this work was to evaluate Azospirillum root association and further colonization through stolons from bacterial inoculated strawberry mother-plants to new born daughter-plants. For that, three commercial cultivars of strawberry ('Camarosa', 'Milsei' and 'Selva') were root inoculated with two strains of A. brasilense: REC3 and PEC5. Scanning and transmission electron microscopy observations showed clear bacterial attachment to the root surface and colonization of root and stolon inner tissues. The diazotrophic bacteria were re-isolated from inoculated mother-plants, non-inoculated daughters and stolons using N-free NFb semisolid medium. In all cases, the MPN from root samples was higher than from stolons. The bacterial nifD gene, essential in the biological N 2-fixing process, was PCR-amplified from DNA of roots and stolons proving the occurrence of diazotrophs within these tissues. To confirm that these bacteria corresponded to the inoculated PGPB, the 16S rDNA gene of re-isolates was subjected to amplified rDNA restriction analysis (ARDRA) and to automated DNA sequencing, revealing that they belong to Azospirillum brasilense. This confirms effective rhizosphere colonization of strawberry mother-plants and also the colonization of A. brasilense to new daughter-plants via stolons. This is the first report about A. brasilense colonization from one strawberry plant to another one by colonizing inner tissues of roots and stolons. This means that a single inoculation with selected PGPB would allow the growers to have numerous plant generations at nursery already inoculated and with better conditions to be planted at field, contributing to a sustainable agricultural practice. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-06 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/195604 Guerrero Molina, María Fernanda; Winik, Beatriz Clara; Pedraza, Raúl Osvaldo; More than rhizosphere colonization of strawberry plants by Azospirillum brasilense; Elsevier Science; Applied Soil Ecology; 61; 6-2012; 205-212 0929-1393 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/195604 |
identifier_str_mv |
Guerrero Molina, María Fernanda; Winik, Beatriz Clara; Pedraza, Raúl Osvaldo; More than rhizosphere colonization of strawberry plants by Azospirillum brasilense; Elsevier Science; Applied Soil Ecology; 61; 6-2012; 205-212 0929-1393 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.apsoil.2011.10.011 info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0929139311002307 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Science |
publisher.none.fl_str_mv |
Elsevier Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842269710240972800 |
score |
13.13397 |