Cytotoxic Compounds from Aerial Organs of Xanthium strumarium

Autores
Piloto Ferrer, Janet; Zampini, Iris Catiana; Cuello, Ana Soledad; Marbelis, Francisco; Romero, Aylema; Valdivia, Dayana; González, María; Salas, Carlos; Sanchez Lamar, Ángel; Isla, Maria Ines
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Xanthium strumarium L., the main species of the genus Xanthium, is ubiquitously distributed. The aim of this study was to determine the cytotoxic effect of aerial organs of X. strumarium, grown in Cuba, against cancer cell lines and the isolation of compounds potentially responsible for this activity. Initially, an ethanol partitioning procedure yielded the XSE extract that was subsequently fractionated with chloroform resulting in a XSCF fraction. Both, XSE and XSCF fractions exhibited cytotoxic effects on MDA MB-231, MCF7, A549 and CT26 cell lines by using the MTT assay. Above all, the XSCF fraction was more active than XSE. For this reason, XSCF was subsequently fractionated by silica gel chromatography and the active fractions submitted to semi-preparative HPLC for isolation of bioactive compounds. Six sub-fractions (SF1 to SF6) were recovered. Sub-fractions 3 and 6 were the most active on each assayed cell line, while sub-fractions 4 and 5 were only active against A549 and CT26 cell lines. In each case, sub-fraction 6 showed the strongest inhibitory effect. The HPLC-DAD fingerprint of sub-fraction 6 showed a single peak that was identified by GC-MS as (-) spathulenol, a sesquiterpene with reported antitumor activity.
Fil: Piloto Ferrer, Janet. Centro de Investigación y Desarrollo de Medicamentos; Cuba
Fil: Zampini, Iris Catiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: Cuello, Ana Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: Marbelis, Francisco. Centro de Investigación y Desarrollo de Medicamentos; Cuba
Fil: Romero, Aylema. Centro de Investigación y Desarrollo de Medicamentos; Cuba
Fil: Valdivia, Dayana. Centro de Investigación y Desarrollo de Medicamentos; Cuba
Fil: González, María. Centro de Investigación y Desarrollo de Medicamentos; Cuba
Fil: Salas, Carlos. Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas; Brasil
Fil: Sanchez Lamar, Ángel. Universidad de La Habana; Cuba
Fil: Isla, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Materia
Toxicity
Xanthium
Cuba
Citotoxicity
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/43684
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/43684
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Cytotoxic Compounds from Aerial Organs of Xanthium strumariumPiloto Ferrer, JanetZampini, Iris CatianaCuello, Ana SoledadMarbelis, FranciscoRomero, AylemaValdivia, DayanaGonzález, MaríaSalas, CarlosSanchez Lamar, ÁngelIsla, Maria InesToxicityXanthiumCubaCitotoxicityhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Xanthium strumarium L., the main species of the genus Xanthium, is ubiquitously distributed. The aim of this study was to determine the cytotoxic effect of aerial organs of X. strumarium, grown in Cuba, against cancer cell lines and the isolation of compounds potentially responsible for this activity. Initially, an ethanol partitioning procedure yielded the XSE extract that was subsequently fractionated with chloroform resulting in a XSCF fraction. Both, XSE and XSCF fractions exhibited cytotoxic effects on MDA MB-231, MCF7, A549 and CT26 cell lines by using the MTT assay. Above all, the XSCF fraction was more active than XSE. For this reason, XSCF was subsequently fractionated by silica gel chromatography and the active fractions submitted to semi-preparative HPLC for isolation of bioactive compounds. Six sub-fractions (SF1 to SF6) were recovered. Sub-fractions 3 and 6 were the most active on each assayed cell line, while sub-fractions 4 and 5 were only active against A549 and CT26 cell lines. In each case, sub-fraction 6 showed the strongest inhibitory effect. The HPLC-DAD fingerprint of sub-fraction 6 showed a single peak that was identified by GC-MS as (-) spathulenol, a sesquiterpene with reported antitumor activity.Fil: Piloto Ferrer, Janet. Centro de Investigación y Desarrollo de Medicamentos; CubaFil: Zampini, Iris Catiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaFil: Cuello, Ana Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaFil: Marbelis, Francisco. Centro de Investigación y Desarrollo de Medicamentos; CubaFil: Romero, Aylema. Centro de Investigación y Desarrollo de Medicamentos; CubaFil: Valdivia, Dayana. Centro de Investigación y Desarrollo de Medicamentos; CubaFil: González, María. Centro de Investigación y Desarrollo de Medicamentos; CubaFil: Salas, Carlos. Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas; BrasilFil: Sanchez Lamar, Ángel. Universidad de La Habana; CubaFil: Isla, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaNatural Products2016-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/43684Piloto Ferrer, Janet; Zampini, Iris Catiana; Cuello, Ana Soledad; Marbelis, Francisco; Romero, Aylema; et al.; Cytotoxic Compounds from Aerial Organs of Xanthium strumarium; Natural Products; Natural Product Communications; 11; 3; 2-2016; 371-3741934-578X1555-9475CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/pdf/10.1177/1934578X1601100313info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:17:04Zoai:ri.conicet.gov.ar:11336/43684instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:17:05.226CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cytotoxic Compounds from Aerial Organs of Xanthium strumarium
title Cytotoxic Compounds from Aerial Organs of Xanthium strumarium
spellingShingle Cytotoxic Compounds from Aerial Organs of Xanthium strumarium
Piloto Ferrer, Janet
Toxicity
Xanthium
Cuba
Citotoxicity
title_short Cytotoxic Compounds from Aerial Organs of Xanthium strumarium
title_full Cytotoxic Compounds from Aerial Organs of Xanthium strumarium
title_fullStr Cytotoxic Compounds from Aerial Organs of Xanthium strumarium
title_full_unstemmed Cytotoxic Compounds from Aerial Organs of Xanthium strumarium
title_sort Cytotoxic Compounds from Aerial Organs of Xanthium strumarium
dc.creator.none.fl_str_mv Piloto Ferrer, Janet
Zampini, Iris Catiana
Cuello, Ana Soledad
Marbelis, Francisco
Romero, Aylema
Valdivia, Dayana
González, María
Salas, Carlos
Sanchez Lamar, Ángel
Isla, Maria Ines
author Piloto Ferrer, Janet
author_facet Piloto Ferrer, Janet
Zampini, Iris Catiana
Cuello, Ana Soledad
Marbelis, Francisco
Romero, Aylema
Valdivia, Dayana
González, María
Salas, Carlos
Sanchez Lamar, Ángel
Isla, Maria Ines
author_role author
author2 Zampini, Iris Catiana
Cuello, Ana Soledad
Marbelis, Francisco
Romero, Aylema
Valdivia, Dayana
González, María
Salas, Carlos
Sanchez Lamar, Ángel
Isla, Maria Ines
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Toxicity
Xanthium
Cuba
Citotoxicity
topic Toxicity
Xanthium
Cuba
Citotoxicity
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Xanthium strumarium L., the main species of the genus Xanthium, is ubiquitously distributed. The aim of this study was to determine the cytotoxic effect of aerial organs of X. strumarium, grown in Cuba, against cancer cell lines and the isolation of compounds potentially responsible for this activity. Initially, an ethanol partitioning procedure yielded the XSE extract that was subsequently fractionated with chloroform resulting in a XSCF fraction. Both, XSE and XSCF fractions exhibited cytotoxic effects on MDA MB-231, MCF7, A549 and CT26 cell lines by using the MTT assay. Above all, the XSCF fraction was more active than XSE. For this reason, XSCF was subsequently fractionated by silica gel chromatography and the active fractions submitted to semi-preparative HPLC for isolation of bioactive compounds. Six sub-fractions (SF1 to SF6) were recovered. Sub-fractions 3 and 6 were the most active on each assayed cell line, while sub-fractions 4 and 5 were only active against A549 and CT26 cell lines. In each case, sub-fraction 6 showed the strongest inhibitory effect. The HPLC-DAD fingerprint of sub-fraction 6 showed a single peak that was identified by GC-MS as (-) spathulenol, a sesquiterpene with reported antitumor activity.
Fil: Piloto Ferrer, Janet. Centro de Investigación y Desarrollo de Medicamentos; Cuba
Fil: Zampini, Iris Catiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: Cuello, Ana Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: Marbelis, Francisco. Centro de Investigación y Desarrollo de Medicamentos; Cuba
Fil: Romero, Aylema. Centro de Investigación y Desarrollo de Medicamentos; Cuba
Fil: Valdivia, Dayana. Centro de Investigación y Desarrollo de Medicamentos; Cuba
Fil: González, María. Centro de Investigación y Desarrollo de Medicamentos; Cuba
Fil: Salas, Carlos. Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas; Brasil
Fil: Sanchez Lamar, Ángel. Universidad de La Habana; Cuba
Fil: Isla, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
description Xanthium strumarium L., the main species of the genus Xanthium, is ubiquitously distributed. The aim of this study was to determine the cytotoxic effect of aerial organs of X. strumarium, grown in Cuba, against cancer cell lines and the isolation of compounds potentially responsible for this activity. Initially, an ethanol partitioning procedure yielded the XSE extract that was subsequently fractionated with chloroform resulting in a XSCF fraction. Both, XSE and XSCF fractions exhibited cytotoxic effects on MDA MB-231, MCF7, A549 and CT26 cell lines by using the MTT assay. Above all, the XSCF fraction was more active than XSE. For this reason, XSCF was subsequently fractionated by silica gel chromatography and the active fractions submitted to semi-preparative HPLC for isolation of bioactive compounds. Six sub-fractions (SF1 to SF6) were recovered. Sub-fractions 3 and 6 were the most active on each assayed cell line, while sub-fractions 4 and 5 were only active against A549 and CT26 cell lines. In each case, sub-fraction 6 showed the strongest inhibitory effect. The HPLC-DAD fingerprint of sub-fraction 6 showed a single peak that was identified by GC-MS as (-) spathulenol, a sesquiterpene with reported antitumor activity.
publishDate 2016
dc.date.none.fl_str_mv 2016-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/43684
Piloto Ferrer, Janet; Zampini, Iris Catiana; Cuello, Ana Soledad; Marbelis, Francisco; Romero, Aylema; et al.; Cytotoxic Compounds from Aerial Organs of Xanthium strumarium; Natural Products; Natural Product Communications; 11; 3; 2-2016; 371-374
1934-578X
1555-9475
CONICET Digital
CONICET
url http://hdl.handle.net/11336/43684
identifier_str_mv Piloto Ferrer, Janet; Zampini, Iris Catiana; Cuello, Ana Soledad; Marbelis, Francisco; Romero, Aylema; et al.; Cytotoxic Compounds from Aerial Organs of Xanthium strumarium; Natural Products; Natural Product Communications; 11; 3; 2-2016; 371-374
1934-578X
1555-9475
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/pdf/10.1177/1934578X1601100313
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Natural Products
publisher.none.fl_str_mv Natural Products
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846781629430759424
score 12.982451

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