Physical Properties and Ionic Concentration of the Bovine Cervical Mucus at the Moment of Inseminating: Spontaneous Estrous and Induced Estrous

Autores
Bernardi, S. F.; Savia, Caren Luciana; de Paz, L. J.; Rodriguez, J; Marini, Pablo Roberto
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objective of the investigation was to characterize biochemically the cervical mucus collected prior to the artificialinsemination of Holstein heifers comparing spontaneous oestrus with induced oestrus. The concentration of sodiumand potassium was determined through flame photometry, chlorine ions by Schoenfeld reaction, magnesium andcalcium was determined through colorimetry in micro plates. pH (reactive tape) and degree of crystallization (opticalmicroscope) were also evaluated. Natural estrous was detected by observing the behavior of the heifers twice a dayand by observing the characteristics of the vulva. In order to induce oestrous, an intra vaginal device was placedwith 1 gm of progesterone and 2 mg of estradiol benzoate were injected (Day 0). On day 8 the device waswithdrawn and 0-150 mg of D-Cloprostenol and 1 mg of Estradiol Cypionate were provided. 56 hours afterwithdrawal, the device was inseminated and at 60 days of pregnancy determined. The cervical mucus of heifers withspontaneous oestrus showed lower content of sodium and potassium, higher magnesium and chlorine, and biggerproportion of sodium/potassium from that obtained by induce oestrus. When comparing the pregnant with the emptyheifers, it was discovered that at the moment of insemination, the pH value and the degree of crystallization ofpregnant heifers was significantly lower, same as the concentrations of potassium and magnesium. Sodium washigher in this group. Pregnancy was always related to high ionic level generating an osmotic force that draws waterto the cervical mucus, characteristic fundamental in transport of sperms.
Fil: Bernardi, S. F.. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias; Argentina
Fil: Savia, Caren Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Saavedra 15. Centro Argentino de Información Científica y Tecnológica; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias; Argentina. Universidad Nacional de Rosario. Secretaria de Ciencia y Técnica. Centro Binacional de Investigación en Criobiología Clínica y Aplicada; Argentina
Fil: de Paz, L. J.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Saavedra 15. Centro Argentino de Información Científica y Tecnológica; Argentina. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Técnica. Centro Binacional (Argentina- Italia) de Investigaciones en Criobiología Clí­nica y Aplicada; Argentina
Fil: Rodriguez, J. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias; Argentina. Universidad Nacional de Rosario. Secretaria de Ciencia y Técnica. Centro Binacional de Investigación en Criobiología Clínica y Aplicada; Argentina
Fil: Marini, Pablo Roberto. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias; Argentina
Materia
HEIFERS
PREGNANCY
IONIC ELECTROLYTES
MUCUS CRYSTALLIZATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/93949
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Physical Properties and Ionic Concentration of the Bovine Cervical Mucus at the Moment of Inseminating: Spontaneous Estrous and Induced EstrousBernardi, S. F.Savia, Caren Lucianade Paz, L. J.Rodriguez, JMarini, Pablo RobertoHEIFERSPREGNANCYIONIC ELECTROLYTESMUCUS CRYSTALLIZATIONhttps://purl.org/becyt/ford/4.2https://purl.org/becyt/ford/4Objective of the investigation was to characterize biochemically the cervical mucus collected prior to the artificialinsemination of Holstein heifers comparing spontaneous oestrus with induced oestrus. The concentration of sodiumand potassium was determined through flame photometry, chlorine ions by Schoenfeld reaction, magnesium andcalcium was determined through colorimetry in micro plates. pH (reactive tape) and degree of crystallization (opticalmicroscope) were also evaluated. Natural estrous was detected by observing the behavior of the heifers twice a dayand by observing the characteristics of the vulva. In order to induce oestrous, an intra vaginal device was placedwith 1 gm of progesterone and 2 mg of estradiol benzoate were injected (Day 0). On day 8 the device waswithdrawn and 0-150 mg of D-Cloprostenol and 1 mg of Estradiol Cypionate were provided. 56 hours afterwithdrawal, the device was inseminated and at 60 days of pregnancy determined. The cervical mucus of heifers withspontaneous oestrus showed lower content of sodium and potassium, higher magnesium and chlorine, and biggerproportion of sodium/potassium from that obtained by induce oestrus. When comparing the pregnant with the emptyheifers, it was discovered that at the moment of insemination, the pH value and the degree of crystallization ofpregnant heifers was significantly lower, same as the concentrations of potassium and magnesium. Sodium washigher in this group. Pregnancy was always related to high ionic level generating an osmotic force that draws waterto the cervical mucus, characteristic fundamental in transport of sperms.Fil: Bernardi, S. F.. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias; ArgentinaFil: Savia, Caren Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Saavedra 15. Centro Argentino de Información Científica y Tecnológica; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias; Argentina. Universidad Nacional de Rosario. Secretaria de Ciencia y Técnica. Centro Binacional de Investigación en Criobiología Clínica y Aplicada; ArgentinaFil: de Paz, L. J.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Saavedra 15. Centro Argentino de Información Científica y Tecnológica; Argentina. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Técnica. Centro Binacional (Argentina- Italia) de Investigaciones en Criobiología Clí­nica y Aplicada; ArgentinaFil: Rodriguez, J. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias; Argentina. Universidad Nacional de Rosario. Secretaria de Ciencia y Técnica. Centro Binacional de Investigación en Criobiología Clínica y Aplicada; ArgentinaFil: Marini, Pablo Roberto. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias; ArgentinaOmics Publishing Group2018-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/93949Bernardi, S. F.; Savia, Caren Luciana; de Paz, L. J.; Rodriguez, J; Marini, Pablo Roberto; Physical Properties and Ionic Concentration of the Bovine Cervical Mucus at the Moment of Inseminating: Spontaneous Estrous and Induced Estrous; Omics Publishing Group; Journal of Veterinary Science & Technology; 9; 3; 5-2018; 2-52157-75792157-7579CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.4172/2157-7579.1000545info:eu-repo/semantics/altIdentifier/url/https://www.omicsonline.org/open-access/physical-properties-and-ionic-concentration-of-the-bovine-cervical-mucus-at-the-moment-of-inseminating-spontaneous-estrous-and-ind-2157-7579-1000545-102955.htmlinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:10:48Zoai:ri.conicet.gov.ar:11336/93949instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:10:48.786CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Physical Properties and Ionic Concentration of the Bovine Cervical Mucus at the Moment of Inseminating: Spontaneous Estrous and Induced Estrous
title Physical Properties and Ionic Concentration of the Bovine Cervical Mucus at the Moment of Inseminating: Spontaneous Estrous and Induced Estrous
spellingShingle Physical Properties and Ionic Concentration of the Bovine Cervical Mucus at the Moment of Inseminating: Spontaneous Estrous and Induced Estrous
Bernardi, S. F.
HEIFERS
PREGNANCY
IONIC ELECTROLYTES
MUCUS CRYSTALLIZATION
title_short Physical Properties and Ionic Concentration of the Bovine Cervical Mucus at the Moment of Inseminating: Spontaneous Estrous and Induced Estrous
title_full Physical Properties and Ionic Concentration of the Bovine Cervical Mucus at the Moment of Inseminating: Spontaneous Estrous and Induced Estrous
title_fullStr Physical Properties and Ionic Concentration of the Bovine Cervical Mucus at the Moment of Inseminating: Spontaneous Estrous and Induced Estrous
title_full_unstemmed Physical Properties and Ionic Concentration of the Bovine Cervical Mucus at the Moment of Inseminating: Spontaneous Estrous and Induced Estrous
title_sort Physical Properties and Ionic Concentration of the Bovine Cervical Mucus at the Moment of Inseminating: Spontaneous Estrous and Induced Estrous
dc.creator.none.fl_str_mv Bernardi, S. F.
Savia, Caren Luciana
de Paz, L. J.
Rodriguez, J
Marini, Pablo Roberto
author Bernardi, S. F.
author_facet Bernardi, S. F.
Savia, Caren Luciana
de Paz, L. J.
Rodriguez, J
Marini, Pablo Roberto
author_role author
author2 Savia, Caren Luciana
de Paz, L. J.
Rodriguez, J
Marini, Pablo Roberto
author2_role author
author
author
author
dc.subject.none.fl_str_mv HEIFERS
PREGNANCY
IONIC ELECTROLYTES
MUCUS CRYSTALLIZATION
topic HEIFERS
PREGNANCY
IONIC ELECTROLYTES
MUCUS CRYSTALLIZATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.2
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Objective of the investigation was to characterize biochemically the cervical mucus collected prior to the artificialinsemination of Holstein heifers comparing spontaneous oestrus with induced oestrus. The concentration of sodiumand potassium was determined through flame photometry, chlorine ions by Schoenfeld reaction, magnesium andcalcium was determined through colorimetry in micro plates. pH (reactive tape) and degree of crystallization (opticalmicroscope) were also evaluated. Natural estrous was detected by observing the behavior of the heifers twice a dayand by observing the characteristics of the vulva. In order to induce oestrous, an intra vaginal device was placedwith 1 gm of progesterone and 2 mg of estradiol benzoate were injected (Day 0). On day 8 the device waswithdrawn and 0-150 mg of D-Cloprostenol and 1 mg of Estradiol Cypionate were provided. 56 hours afterwithdrawal, the device was inseminated and at 60 days of pregnancy determined. The cervical mucus of heifers withspontaneous oestrus showed lower content of sodium and potassium, higher magnesium and chlorine, and biggerproportion of sodium/potassium from that obtained by induce oestrus. When comparing the pregnant with the emptyheifers, it was discovered that at the moment of insemination, the pH value and the degree of crystallization ofpregnant heifers was significantly lower, same as the concentrations of potassium and magnesium. Sodium washigher in this group. Pregnancy was always related to high ionic level generating an osmotic force that draws waterto the cervical mucus, characteristic fundamental in transport of sperms.
Fil: Bernardi, S. F.. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias; Argentina
Fil: Savia, Caren Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Saavedra 15. Centro Argentino de Información Científica y Tecnológica; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias; Argentina. Universidad Nacional de Rosario. Secretaria de Ciencia y Técnica. Centro Binacional de Investigación en Criobiología Clínica y Aplicada; Argentina
Fil: de Paz, L. J.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Saavedra 15. Centro Argentino de Información Científica y Tecnológica; Argentina. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Técnica. Centro Binacional (Argentina- Italia) de Investigaciones en Criobiología Clí­nica y Aplicada; Argentina
Fil: Rodriguez, J. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias; Argentina. Universidad Nacional de Rosario. Secretaria de Ciencia y Técnica. Centro Binacional de Investigación en Criobiología Clínica y Aplicada; Argentina
Fil: Marini, Pablo Roberto. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias; Argentina
description Objective of the investigation was to characterize biochemically the cervical mucus collected prior to the artificialinsemination of Holstein heifers comparing spontaneous oestrus with induced oestrus. The concentration of sodiumand potassium was determined through flame photometry, chlorine ions by Schoenfeld reaction, magnesium andcalcium was determined through colorimetry in micro plates. pH (reactive tape) and degree of crystallization (opticalmicroscope) were also evaluated. Natural estrous was detected by observing the behavior of the heifers twice a dayand by observing the characteristics of the vulva. In order to induce oestrous, an intra vaginal device was placedwith 1 gm of progesterone and 2 mg of estradiol benzoate were injected (Day 0). On day 8 the device waswithdrawn and 0-150 mg of D-Cloprostenol and 1 mg of Estradiol Cypionate were provided. 56 hours afterwithdrawal, the device was inseminated and at 60 days of pregnancy determined. The cervical mucus of heifers withspontaneous oestrus showed lower content of sodium and potassium, higher magnesium and chlorine, and biggerproportion of sodium/potassium from that obtained by induce oestrus. When comparing the pregnant with the emptyheifers, it was discovered that at the moment of insemination, the pH value and the degree of crystallization ofpregnant heifers was significantly lower, same as the concentrations of potassium and magnesium. Sodium washigher in this group. Pregnancy was always related to high ionic level generating an osmotic force that draws waterto the cervical mucus, characteristic fundamental in transport of sperms.
publishDate 2018
dc.date.none.fl_str_mv 2018-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/93949
Bernardi, S. F.; Savia, Caren Luciana; de Paz, L. J.; Rodriguez, J; Marini, Pablo Roberto; Physical Properties and Ionic Concentration of the Bovine Cervical Mucus at the Moment of Inseminating: Spontaneous Estrous and Induced Estrous; Omics Publishing Group; Journal of Veterinary Science & Technology; 9; 3; 5-2018; 2-5
2157-7579
2157-7579
CONICET Digital
CONICET
url http://hdl.handle.net/11336/93949
identifier_str_mv Bernardi, S. F.; Savia, Caren Luciana; de Paz, L. J.; Rodriguez, J; Marini, Pablo Roberto; Physical Properties and Ionic Concentration of the Bovine Cervical Mucus at the Moment of Inseminating: Spontaneous Estrous and Induced Estrous; Omics Publishing Group; Journal of Veterinary Science & Technology; 9; 3; 5-2018; 2-5
2157-7579
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.4172/2157-7579.1000545
info:eu-repo/semantics/altIdentifier/url/https://www.omicsonline.org/open-access/physical-properties-and-ionic-concentration-of-the-bovine-cervical-mucus-at-the-moment-of-inseminating-spontaneous-estrous-and-ind-2157-7579-1000545-102955.html
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Omics Publishing Group
publisher.none.fl_str_mv Omics Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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