A large-scale 19F MRI-based cell migration assay to optimize cell therapy

Autores
Bonetto, Fernando Jose; Srinivas, M.; Weigelin, B.; Cruz, L. J.; Heerschap, A.; Friedl, P.; Figdor, C. G.; de Vries, I. J. M.
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Adoptive transfer of cells for therapeutic purposes requires efficient and precise delivery to the target organ whilst preserving cell function. Therefore, therapeutically applied cells need to migrate and integrate within their target tissues after delivery, e.g. dendritic cells (DCs) need to migrate to lymph nodes to elicit an antigen-specific immune response. Previous studies have shown that inappropriate cell delivery can hinder DC migration and result in insufficient immune induction. As migration can be extremely difficult to study quantitatively in vivo, we propose an in vitro assay that reproduces key in vivo conditions to optimize cell delivery and migration in vivo. Using DC migration along a chemokine gradient, we describe here a novel 19 F MR-based, large-scale, quantitative assay to measure cell migration in a three-dimensional collagen scaffold. Unlike conventional migration assays, this set-up is amenable to both large and small cell numbers, as well as opaque tissue samples and the inclusion of chemokines or other factors. We labeled primary human DCs with a 19 F label suitable for clinical use; (0.5–15) × 106 cells in the scaffolds were imaged sequentially, and migration was assessed using two independent methods. We found no migration with larger numbers of cells, but up to 3% with less than one million cells. Hence, we show that the cell density in cell bolus injections has a decisive impact on migration, and this may explain the limited migration observed using large cell numbers in the clinic.
Fil: Bonetto, Fernando Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); Argentina. Radboud University Nijmegen Medical Center; Países Bajos
Fil: Srinivas, M.. Radboud University Nijmegen Medical Center; Países Bajos
Fil: Weigelin, B.. Radboud University Nijmegen Medical Center; Países Bajos
Fil: Cruz, L. J.. Radboud University Nijmegen Medical Center; Países Bajos
Fil: Heerschap, A.. Radboud University Nijmegen Medical Center; Países Bajos
Fil: Friedl, P.. Radboud University Nijmegen Medical Center; Países Bajos
Fil: Figdor, C. G.. Radboud University Nijmegen Medical Center; Países Bajos
Fil: de Vries, I. J. M.. Radboud University Nijmegen Medical Center; Países Bajos
Materia
19f Magnetic Resonance Imaging
Cell Migration
Migration Assays
Dendritic Cell Immunotherapy
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/10726

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network_name_str CONICET Digital (CONICET)
spelling A large-scale 19F MRI-based cell migration assay to optimize cell therapyBonetto, Fernando JoseSrinivas, M.Weigelin, B.Cruz, L. J.Heerschap, A.Friedl, P.Figdor, C. G.de Vries, I. J. M.19f Magnetic Resonance ImagingCell MigrationMigration AssaysDendritic Cell Immunotherapyhttps://purl.org/becyt/ford/1.3https://purl.org/becyt/ford/1Adoptive transfer of cells for therapeutic purposes requires efficient and precise delivery to the target organ whilst preserving cell function. Therefore, therapeutically applied cells need to migrate and integrate within their target tissues after delivery, e.g. dendritic cells (DCs) need to migrate to lymph nodes to elicit an antigen-specific immune response. Previous studies have shown that inappropriate cell delivery can hinder DC migration and result in insufficient immune induction. As migration can be extremely difficult to study quantitatively in vivo, we propose an in vitro assay that reproduces key in vivo conditions to optimize cell delivery and migration in vivo. Using DC migration along a chemokine gradient, we describe here a novel 19 F MR-based, large-scale, quantitative assay to measure cell migration in a three-dimensional collagen scaffold. Unlike conventional migration assays, this set-up is amenable to both large and small cell numbers, as well as opaque tissue samples and the inclusion of chemokines or other factors. We labeled primary human DCs with a 19 F label suitable for clinical use; (0.5–15) × 106 cells in the scaffolds were imaged sequentially, and migration was assessed using two independent methods. We found no migration with larger numbers of cells, but up to 3% with less than one million cells. Hence, we show that the cell density in cell bolus injections has a decisive impact on migration, and this may explain the limited migration observed using large cell numbers in the clinic.Fil: Bonetto, Fernando Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); Argentina. Radboud University Nijmegen Medical Center; Países BajosFil: Srinivas, M.. Radboud University Nijmegen Medical Center; Países BajosFil: Weigelin, B.. Radboud University Nijmegen Medical Center; Países BajosFil: Cruz, L. J.. Radboud University Nijmegen Medical Center; Países BajosFil: Heerschap, A.. Radboud University Nijmegen Medical Center; Países BajosFil: Friedl, P.. Radboud University Nijmegen Medical Center; Países BajosFil: Figdor, C. G.. Radboud University Nijmegen Medical Center; Países BajosFil: de Vries, I. J. M.. Radboud University Nijmegen Medical Center; Países BajosWiley2012-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/10726Bonetto, Fernando Jose; Srinivas, M.; Weigelin, B.; Cruz, L. J.; Heerschap, A.; et al.; A large-scale 19F MRI-based cell migration assay to optimize cell therapy; Wiley; Nmr In Biomedicine; 25; 1-2012; 1095-11030952-3480enginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/nbm.2774/abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1002/nbm.2774info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:51:59Zoai:ri.conicet.gov.ar:11336/10726instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:52:00.138CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A large-scale 19F MRI-based cell migration assay to optimize cell therapy
title A large-scale 19F MRI-based cell migration assay to optimize cell therapy
spellingShingle A large-scale 19F MRI-based cell migration assay to optimize cell therapy
Bonetto, Fernando Jose
19f Magnetic Resonance Imaging
Cell Migration
Migration Assays
Dendritic Cell Immunotherapy
title_short A large-scale 19F MRI-based cell migration assay to optimize cell therapy
title_full A large-scale 19F MRI-based cell migration assay to optimize cell therapy
title_fullStr A large-scale 19F MRI-based cell migration assay to optimize cell therapy
title_full_unstemmed A large-scale 19F MRI-based cell migration assay to optimize cell therapy
title_sort A large-scale 19F MRI-based cell migration assay to optimize cell therapy
dc.creator.none.fl_str_mv Bonetto, Fernando Jose
Srinivas, M.
Weigelin, B.
Cruz, L. J.
Heerschap, A.
Friedl, P.
Figdor, C. G.
de Vries, I. J. M.
author Bonetto, Fernando Jose
author_facet Bonetto, Fernando Jose
Srinivas, M.
Weigelin, B.
Cruz, L. J.
Heerschap, A.
Friedl, P.
Figdor, C. G.
de Vries, I. J. M.
author_role author
author2 Srinivas, M.
Weigelin, B.
Cruz, L. J.
Heerschap, A.
Friedl, P.
Figdor, C. G.
de Vries, I. J. M.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv 19f Magnetic Resonance Imaging
Cell Migration
Migration Assays
Dendritic Cell Immunotherapy
topic 19f Magnetic Resonance Imaging
Cell Migration
Migration Assays
Dendritic Cell Immunotherapy
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.3
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Adoptive transfer of cells for therapeutic purposes requires efficient and precise delivery to the target organ whilst preserving cell function. Therefore, therapeutically applied cells need to migrate and integrate within their target tissues after delivery, e.g. dendritic cells (DCs) need to migrate to lymph nodes to elicit an antigen-specific immune response. Previous studies have shown that inappropriate cell delivery can hinder DC migration and result in insufficient immune induction. As migration can be extremely difficult to study quantitatively in vivo, we propose an in vitro assay that reproduces key in vivo conditions to optimize cell delivery and migration in vivo. Using DC migration along a chemokine gradient, we describe here a novel 19 F MR-based, large-scale, quantitative assay to measure cell migration in a three-dimensional collagen scaffold. Unlike conventional migration assays, this set-up is amenable to both large and small cell numbers, as well as opaque tissue samples and the inclusion of chemokines or other factors. We labeled primary human DCs with a 19 F label suitable for clinical use; (0.5–15) × 106 cells in the scaffolds were imaged sequentially, and migration was assessed using two independent methods. We found no migration with larger numbers of cells, but up to 3% with less than one million cells. Hence, we show that the cell density in cell bolus injections has a decisive impact on migration, and this may explain the limited migration observed using large cell numbers in the clinic.
Fil: Bonetto, Fernando Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); Argentina. Radboud University Nijmegen Medical Center; Países Bajos
Fil: Srinivas, M.. Radboud University Nijmegen Medical Center; Países Bajos
Fil: Weigelin, B.. Radboud University Nijmegen Medical Center; Países Bajos
Fil: Cruz, L. J.. Radboud University Nijmegen Medical Center; Países Bajos
Fil: Heerschap, A.. Radboud University Nijmegen Medical Center; Países Bajos
Fil: Friedl, P.. Radboud University Nijmegen Medical Center; Países Bajos
Fil: Figdor, C. G.. Radboud University Nijmegen Medical Center; Países Bajos
Fil: de Vries, I. J. M.. Radboud University Nijmegen Medical Center; Países Bajos
description Adoptive transfer of cells for therapeutic purposes requires efficient and precise delivery to the target organ whilst preserving cell function. Therefore, therapeutically applied cells need to migrate and integrate within their target tissues after delivery, e.g. dendritic cells (DCs) need to migrate to lymph nodes to elicit an antigen-specific immune response. Previous studies have shown that inappropriate cell delivery can hinder DC migration and result in insufficient immune induction. As migration can be extremely difficult to study quantitatively in vivo, we propose an in vitro assay that reproduces key in vivo conditions to optimize cell delivery and migration in vivo. Using DC migration along a chemokine gradient, we describe here a novel 19 F MR-based, large-scale, quantitative assay to measure cell migration in a three-dimensional collagen scaffold. Unlike conventional migration assays, this set-up is amenable to both large and small cell numbers, as well as opaque tissue samples and the inclusion of chemokines or other factors. We labeled primary human DCs with a 19 F label suitable for clinical use; (0.5–15) × 106 cells in the scaffolds were imaged sequentially, and migration was assessed using two independent methods. We found no migration with larger numbers of cells, but up to 3% with less than one million cells. Hence, we show that the cell density in cell bolus injections has a decisive impact on migration, and this may explain the limited migration observed using large cell numbers in the clinic.
publishDate 2012
dc.date.none.fl_str_mv 2012-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/10726
Bonetto, Fernando Jose; Srinivas, M.; Weigelin, B.; Cruz, L. J.; Heerschap, A.; et al.; A large-scale 19F MRI-based cell migration assay to optimize cell therapy; Wiley; Nmr In Biomedicine; 25; 1-2012; 1095-1103
0952-3480
url http://hdl.handle.net/11336/10726
identifier_str_mv Bonetto, Fernando Jose; Srinivas, M.; Weigelin, B.; Cruz, L. J.; Heerschap, A.; et al.; A large-scale 19F MRI-based cell migration assay to optimize cell therapy; Wiley; Nmr In Biomedicine; 25; 1-2012; 1095-1103
0952-3480
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/nbm.2774/abstract
info:eu-repo/semantics/altIdentifier/doi/10.1002/nbm.2774
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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