A large-scale 19F MRI-based cell migration assay to optimize cell therapy
- Autores
- Bonetto, Fernando Jose; Srinivas, M.; Weigelin, B.; Cruz, L. J.; Heerschap, A.; Friedl, P.; Figdor, C. G.; de Vries, I. J. M.
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Adoptive transfer of cells for therapeutic purposes requires efficient and precise delivery to the target organ whilst preserving cell function. Therefore, therapeutically applied cells need to migrate and integrate within their target tissues after delivery, e.g. dendritic cells (DCs) need to migrate to lymph nodes to elicit an antigen-specific immune response. Previous studies have shown that inappropriate cell delivery can hinder DC migration and result in insufficient immune induction. As migration can be extremely difficult to study quantitatively in vivo, we propose an in vitro assay that reproduces key in vivo conditions to optimize cell delivery and migration in vivo. Using DC migration along a chemokine gradient, we describe here a novel 19 F MR-based, large-scale, quantitative assay to measure cell migration in a three-dimensional collagen scaffold. Unlike conventional migration assays, this set-up is amenable to both large and small cell numbers, as well as opaque tissue samples and the inclusion of chemokines or other factors. We labeled primary human DCs with a 19 F label suitable for clinical use; (0.5–15) × 106 cells in the scaffolds were imaged sequentially, and migration was assessed using two independent methods. We found no migration with larger numbers of cells, but up to 3% with less than one million cells. Hence, we show that the cell density in cell bolus injections has a decisive impact on migration, and this may explain the limited migration observed using large cell numbers in the clinic.
Fil: Bonetto, Fernando Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); Argentina. Radboud University Nijmegen Medical Center; Países Bajos
Fil: Srinivas, M.. Radboud University Nijmegen Medical Center; Países Bajos
Fil: Weigelin, B.. Radboud University Nijmegen Medical Center; Países Bajos
Fil: Cruz, L. J.. Radboud University Nijmegen Medical Center; Países Bajos
Fil: Heerschap, A.. Radboud University Nijmegen Medical Center; Países Bajos
Fil: Friedl, P.. Radboud University Nijmegen Medical Center; Países Bajos
Fil: Figdor, C. G.. Radboud University Nijmegen Medical Center; Países Bajos
Fil: de Vries, I. J. M.. Radboud University Nijmegen Medical Center; Países Bajos - Materia
-
19f Magnetic Resonance Imaging
Cell Migration
Migration Assays
Dendritic Cell Immunotherapy - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/10726
Ver los metadatos del registro completo
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A large-scale 19F MRI-based cell migration assay to optimize cell therapyBonetto, Fernando JoseSrinivas, M.Weigelin, B.Cruz, L. J.Heerschap, A.Friedl, P.Figdor, C. G.de Vries, I. J. M.19f Magnetic Resonance ImagingCell MigrationMigration AssaysDendritic Cell Immunotherapyhttps://purl.org/becyt/ford/1.3https://purl.org/becyt/ford/1Adoptive transfer of cells for therapeutic purposes requires efficient and precise delivery to the target organ whilst preserving cell function. Therefore, therapeutically applied cells need to migrate and integrate within their target tissues after delivery, e.g. dendritic cells (DCs) need to migrate to lymph nodes to elicit an antigen-specific immune response. Previous studies have shown that inappropriate cell delivery can hinder DC migration and result in insufficient immune induction. As migration can be extremely difficult to study quantitatively in vivo, we propose an in vitro assay that reproduces key in vivo conditions to optimize cell delivery and migration in vivo. Using DC migration along a chemokine gradient, we describe here a novel 19 F MR-based, large-scale, quantitative assay to measure cell migration in a three-dimensional collagen scaffold. Unlike conventional migration assays, this set-up is amenable to both large and small cell numbers, as well as opaque tissue samples and the inclusion of chemokines or other factors. We labeled primary human DCs with a 19 F label suitable for clinical use; (0.5–15) × 106 cells in the scaffolds were imaged sequentially, and migration was assessed using two independent methods. We found no migration with larger numbers of cells, but up to 3% with less than one million cells. Hence, we show that the cell density in cell bolus injections has a decisive impact on migration, and this may explain the limited migration observed using large cell numbers in the clinic.Fil: Bonetto, Fernando Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); Argentina. Radboud University Nijmegen Medical Center; Países BajosFil: Srinivas, M.. Radboud University Nijmegen Medical Center; Países BajosFil: Weigelin, B.. Radboud University Nijmegen Medical Center; Países BajosFil: Cruz, L. J.. Radboud University Nijmegen Medical Center; Países BajosFil: Heerschap, A.. Radboud University Nijmegen Medical Center; Países BajosFil: Friedl, P.. Radboud University Nijmegen Medical Center; Países BajosFil: Figdor, C. G.. Radboud University Nijmegen Medical Center; Países BajosFil: de Vries, I. J. M.. Radboud University Nijmegen Medical Center; Países BajosWiley2012-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/10726Bonetto, Fernando Jose; Srinivas, M.; Weigelin, B.; Cruz, L. J.; Heerschap, A.; et al.; A large-scale 19F MRI-based cell migration assay to optimize cell therapy; Wiley; Nmr In Biomedicine; 25; 1-2012; 1095-11030952-3480enginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/nbm.2774/abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1002/nbm.2774info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:51:59Zoai:ri.conicet.gov.ar:11336/10726instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:52:00.138CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
A large-scale 19F MRI-based cell migration assay to optimize cell therapy |
title |
A large-scale 19F MRI-based cell migration assay to optimize cell therapy |
spellingShingle |
A large-scale 19F MRI-based cell migration assay to optimize cell therapy Bonetto, Fernando Jose 19f Magnetic Resonance Imaging Cell Migration Migration Assays Dendritic Cell Immunotherapy |
title_short |
A large-scale 19F MRI-based cell migration assay to optimize cell therapy |
title_full |
A large-scale 19F MRI-based cell migration assay to optimize cell therapy |
title_fullStr |
A large-scale 19F MRI-based cell migration assay to optimize cell therapy |
title_full_unstemmed |
A large-scale 19F MRI-based cell migration assay to optimize cell therapy |
title_sort |
A large-scale 19F MRI-based cell migration assay to optimize cell therapy |
dc.creator.none.fl_str_mv |
Bonetto, Fernando Jose Srinivas, M. Weigelin, B. Cruz, L. J. Heerschap, A. Friedl, P. Figdor, C. G. de Vries, I. J. M. |
author |
Bonetto, Fernando Jose |
author_facet |
Bonetto, Fernando Jose Srinivas, M. Weigelin, B. Cruz, L. J. Heerschap, A. Friedl, P. Figdor, C. G. de Vries, I. J. M. |
author_role |
author |
author2 |
Srinivas, M. Weigelin, B. Cruz, L. J. Heerschap, A. Friedl, P. Figdor, C. G. de Vries, I. J. M. |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
19f Magnetic Resonance Imaging Cell Migration Migration Assays Dendritic Cell Immunotherapy |
topic |
19f Magnetic Resonance Imaging Cell Migration Migration Assays Dendritic Cell Immunotherapy |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.3 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Adoptive transfer of cells for therapeutic purposes requires efficient and precise delivery to the target organ whilst preserving cell function. Therefore, therapeutically applied cells need to migrate and integrate within their target tissues after delivery, e.g. dendritic cells (DCs) need to migrate to lymph nodes to elicit an antigen-specific immune response. Previous studies have shown that inappropriate cell delivery can hinder DC migration and result in insufficient immune induction. As migration can be extremely difficult to study quantitatively in vivo, we propose an in vitro assay that reproduces key in vivo conditions to optimize cell delivery and migration in vivo. Using DC migration along a chemokine gradient, we describe here a novel 19 F MR-based, large-scale, quantitative assay to measure cell migration in a three-dimensional collagen scaffold. Unlike conventional migration assays, this set-up is amenable to both large and small cell numbers, as well as opaque tissue samples and the inclusion of chemokines or other factors. We labeled primary human DCs with a 19 F label suitable for clinical use; (0.5–15) × 106 cells in the scaffolds were imaged sequentially, and migration was assessed using two independent methods. We found no migration with larger numbers of cells, but up to 3% with less than one million cells. Hence, we show that the cell density in cell bolus injections has a decisive impact on migration, and this may explain the limited migration observed using large cell numbers in the clinic. Fil: Bonetto, Fernando Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); Argentina. Radboud University Nijmegen Medical Center; Países Bajos Fil: Srinivas, M.. Radboud University Nijmegen Medical Center; Países Bajos Fil: Weigelin, B.. Radboud University Nijmegen Medical Center; Países Bajos Fil: Cruz, L. J.. Radboud University Nijmegen Medical Center; Países Bajos Fil: Heerschap, A.. Radboud University Nijmegen Medical Center; Países Bajos Fil: Friedl, P.. Radboud University Nijmegen Medical Center; Países Bajos Fil: Figdor, C. G.. Radboud University Nijmegen Medical Center; Países Bajos Fil: de Vries, I. J. M.. Radboud University Nijmegen Medical Center; Países Bajos |
description |
Adoptive transfer of cells for therapeutic purposes requires efficient and precise delivery to the target organ whilst preserving cell function. Therefore, therapeutically applied cells need to migrate and integrate within their target tissues after delivery, e.g. dendritic cells (DCs) need to migrate to lymph nodes to elicit an antigen-specific immune response. Previous studies have shown that inappropriate cell delivery can hinder DC migration and result in insufficient immune induction. As migration can be extremely difficult to study quantitatively in vivo, we propose an in vitro assay that reproduces key in vivo conditions to optimize cell delivery and migration in vivo. Using DC migration along a chemokine gradient, we describe here a novel 19 F MR-based, large-scale, quantitative assay to measure cell migration in a three-dimensional collagen scaffold. Unlike conventional migration assays, this set-up is amenable to both large and small cell numbers, as well as opaque tissue samples and the inclusion of chemokines or other factors. We labeled primary human DCs with a 19 F label suitable for clinical use; (0.5–15) × 106 cells in the scaffolds were imaged sequentially, and migration was assessed using two independent methods. We found no migration with larger numbers of cells, but up to 3% with less than one million cells. Hence, we show that the cell density in cell bolus injections has a decisive impact on migration, and this may explain the limited migration observed using large cell numbers in the clinic. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-01 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/10726 Bonetto, Fernando Jose; Srinivas, M.; Weigelin, B.; Cruz, L. J.; Heerschap, A.; et al.; A large-scale 19F MRI-based cell migration assay to optimize cell therapy; Wiley; Nmr In Biomedicine; 25; 1-2012; 1095-1103 0952-3480 |
url |
http://hdl.handle.net/11336/10726 |
identifier_str_mv |
Bonetto, Fernando Jose; Srinivas, M.; Weigelin, B.; Cruz, L. J.; Heerschap, A.; et al.; A large-scale 19F MRI-based cell migration assay to optimize cell therapy; Wiley; Nmr In Biomedicine; 25; 1-2012; 1095-1103 0952-3480 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/nbm.2774/abstract info:eu-repo/semantics/altIdentifier/doi/10.1002/nbm.2774 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |