Recombineering: A powerful tool for modification of bacteriophage genomes.

Autores
Marinelli, Laura J.; Hatfull, Graham F.; Piuri, Mariana
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Recombineering, a recently developed technique for efficient genetic manipulation of bacteria, is facilitated by phagederived recombination proteins and has the advantage of using DNA substrates with short regions of homology. This system was first developed in Escherichia coli but has since been adapted for use in other bacteria. It is now widely used in a number of different systems for a variety of purposes, and the construction of chromosomal gene knockouts, deletions, insertions, point mutations, as well as in vivo cloning, mutagenesis of bacterial artificial chromosomes and phasmids, and the construction of genomic libraries has been reported. However, these methods also can be effectively applied to the genetic modification of bacteriophage genomes, in both their prophage and lytically growing states. The ever-growing collection of fully sequenced bacteriophages raises more questions than they answer, including the unknown functions of vast numbers of genes with no known homologs and of unknown function. Recombineering of phage genomes is central to addressing these questions, enabling the simple construction of mutants, determination of gene essentiality, and elucidation of gene function. In turn, advances in our understanding of phage genomics should present similar recombineering tools for dissecting a multitude of other genetically naïve bacterial systems.
Fil: Marinelli, Laura J.. University of California; Estados Unidos
Fil: Hatfull, Graham F.. University of Pittsburgh; Estados Unidos
Fil: Piuri, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Materia
BACTERIOPHAGE
RECOMBINEERING
BRED
MUTANT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/268961

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spelling Recombineering: A powerful tool for modification of bacteriophage genomes.Marinelli, Laura J.Hatfull, Graham F.Piuri, MarianaBACTERIOPHAGERECOMBINEERINGBREDMUTANThttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Recombineering, a recently developed technique for efficient genetic manipulation of bacteria, is facilitated by phagederived recombination proteins and has the advantage of using DNA substrates with short regions of homology. This system was first developed in Escherichia coli but has since been adapted for use in other bacteria. It is now widely used in a number of different systems for a variety of purposes, and the construction of chromosomal gene knockouts, deletions, insertions, point mutations, as well as in vivo cloning, mutagenesis of bacterial artificial chromosomes and phasmids, and the construction of genomic libraries has been reported. However, these methods also can be effectively applied to the genetic modification of bacteriophage genomes, in both their prophage and lytically growing states. The ever-growing collection of fully sequenced bacteriophages raises more questions than they answer, including the unknown functions of vast numbers of genes with no known homologs and of unknown function. Recombineering of phage genomes is central to addressing these questions, enabling the simple construction of mutants, determination of gene essentiality, and elucidation of gene function. In turn, advances in our understanding of phage genomics should present similar recombineering tools for dissecting a multitude of other genetically naïve bacterial systems.Fil: Marinelli, Laura J.. University of California; Estados UnidosFil: Hatfull, Graham F.. University of Pittsburgh; Estados UnidosFil: Piuri, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaLandes Bioscience2012-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/268961Marinelli, Laura J.; Hatfull, Graham F.; Piuri, Mariana; Recombineering: A powerful tool for modification of bacteriophage genomes.; Landes Bioscience; Bacteriophage; 2; 1; 1-2012; 5-142159-70732159-7081CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/10.4161/bact.18778info:eu-repo/semantics/altIdentifier/doi/10.4161/bact.18778info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:07:49Zoai:ri.conicet.gov.ar:11336/268961instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:07:49.87CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Recombineering: A powerful tool for modification of bacteriophage genomes.
title Recombineering: A powerful tool for modification of bacteriophage genomes.
spellingShingle Recombineering: A powerful tool for modification of bacteriophage genomes.
Marinelli, Laura J.
BACTERIOPHAGE
RECOMBINEERING
BRED
MUTANT
title_short Recombineering: A powerful tool for modification of bacteriophage genomes.
title_full Recombineering: A powerful tool for modification of bacteriophage genomes.
title_fullStr Recombineering: A powerful tool for modification of bacteriophage genomes.
title_full_unstemmed Recombineering: A powerful tool for modification of bacteriophage genomes.
title_sort Recombineering: A powerful tool for modification of bacteriophage genomes.
dc.creator.none.fl_str_mv Marinelli, Laura J.
Hatfull, Graham F.
Piuri, Mariana
author Marinelli, Laura J.
author_facet Marinelli, Laura J.
Hatfull, Graham F.
Piuri, Mariana
author_role author
author2 Hatfull, Graham F.
Piuri, Mariana
author2_role author
author
dc.subject.none.fl_str_mv BACTERIOPHAGE
RECOMBINEERING
BRED
MUTANT
topic BACTERIOPHAGE
RECOMBINEERING
BRED
MUTANT
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Recombineering, a recently developed technique for efficient genetic manipulation of bacteria, is facilitated by phagederived recombination proteins and has the advantage of using DNA substrates with short regions of homology. This system was first developed in Escherichia coli but has since been adapted for use in other bacteria. It is now widely used in a number of different systems for a variety of purposes, and the construction of chromosomal gene knockouts, deletions, insertions, point mutations, as well as in vivo cloning, mutagenesis of bacterial artificial chromosomes and phasmids, and the construction of genomic libraries has been reported. However, these methods also can be effectively applied to the genetic modification of bacteriophage genomes, in both their prophage and lytically growing states. The ever-growing collection of fully sequenced bacteriophages raises more questions than they answer, including the unknown functions of vast numbers of genes with no known homologs and of unknown function. Recombineering of phage genomes is central to addressing these questions, enabling the simple construction of mutants, determination of gene essentiality, and elucidation of gene function. In turn, advances in our understanding of phage genomics should present similar recombineering tools for dissecting a multitude of other genetically naïve bacterial systems.
Fil: Marinelli, Laura J.. University of California; Estados Unidos
Fil: Hatfull, Graham F.. University of Pittsburgh; Estados Unidos
Fil: Piuri, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
description Recombineering, a recently developed technique for efficient genetic manipulation of bacteria, is facilitated by phagederived recombination proteins and has the advantage of using DNA substrates with short regions of homology. This system was first developed in Escherichia coli but has since been adapted for use in other bacteria. It is now widely used in a number of different systems for a variety of purposes, and the construction of chromosomal gene knockouts, deletions, insertions, point mutations, as well as in vivo cloning, mutagenesis of bacterial artificial chromosomes and phasmids, and the construction of genomic libraries has been reported. However, these methods also can be effectively applied to the genetic modification of bacteriophage genomes, in both their prophage and lytically growing states. The ever-growing collection of fully sequenced bacteriophages raises more questions than they answer, including the unknown functions of vast numbers of genes with no known homologs and of unknown function. Recombineering of phage genomes is central to addressing these questions, enabling the simple construction of mutants, determination of gene essentiality, and elucidation of gene function. In turn, advances in our understanding of phage genomics should present similar recombineering tools for dissecting a multitude of other genetically naïve bacterial systems.
publishDate 2012
dc.date.none.fl_str_mv 2012-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/268961
Marinelli, Laura J.; Hatfull, Graham F.; Piuri, Mariana; Recombineering: A powerful tool for modification of bacteriophage genomes.; Landes Bioscience; Bacteriophage; 2; 1; 1-2012; 5-14
2159-7073
2159-7081
CONICET Digital
CONICET
url http://hdl.handle.net/11336/268961
identifier_str_mv Marinelli, Laura J.; Hatfull, Graham F.; Piuri, Mariana; Recombineering: A powerful tool for modification of bacteriophage genomes.; Landes Bioscience; Bacteriophage; 2; 1; 1-2012; 5-14
2159-7073
2159-7081
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/10.4161/bact.18778
info:eu-repo/semantics/altIdentifier/doi/10.4161/bact.18778
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Landes Bioscience
publisher.none.fl_str_mv Landes Bioscience
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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