Recombineering: A powerful tool for modification of bacteriophage genomes.
- Autores
- Marinelli, Laura J.; Hatfull, Graham F.; Piuri, Mariana
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Recombineering, a recently developed technique for efficient genetic manipulation of bacteria, is facilitated by phagederived recombination proteins and has the advantage of using DNA substrates with short regions of homology. This system was first developed in Escherichia coli but has since been adapted for use in other bacteria. It is now widely used in a number of different systems for a variety of purposes, and the construction of chromosomal gene knockouts, deletions, insertions, point mutations, as well as in vivo cloning, mutagenesis of bacterial artificial chromosomes and phasmids, and the construction of genomic libraries has been reported. However, these methods also can be effectively applied to the genetic modification of bacteriophage genomes, in both their prophage and lytically growing states. The ever-growing collection of fully sequenced bacteriophages raises more questions than they answer, including the unknown functions of vast numbers of genes with no known homologs and of unknown function. Recombineering of phage genomes is central to addressing these questions, enabling the simple construction of mutants, determination of gene essentiality, and elucidation of gene function. In turn, advances in our understanding of phage genomics should present similar recombineering tools for dissecting a multitude of other genetically naïve bacterial systems.
Fil: Marinelli, Laura J.. University of California; Estados Unidos
Fil: Hatfull, Graham F.. University of Pittsburgh; Estados Unidos
Fil: Piuri, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina - Materia
-
BACTERIOPHAGE
RECOMBINEERING
BRED
MUTANT - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/268961
Ver los metadatos del registro completo
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CONICET Digital (CONICET) |
spelling |
Recombineering: A powerful tool for modification of bacteriophage genomes.Marinelli, Laura J.Hatfull, Graham F.Piuri, MarianaBACTERIOPHAGERECOMBINEERINGBREDMUTANThttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Recombineering, a recently developed technique for efficient genetic manipulation of bacteria, is facilitated by phagederived recombination proteins and has the advantage of using DNA substrates with short regions of homology. This system was first developed in Escherichia coli but has since been adapted for use in other bacteria. It is now widely used in a number of different systems for a variety of purposes, and the construction of chromosomal gene knockouts, deletions, insertions, point mutations, as well as in vivo cloning, mutagenesis of bacterial artificial chromosomes and phasmids, and the construction of genomic libraries has been reported. However, these methods also can be effectively applied to the genetic modification of bacteriophage genomes, in both their prophage and lytically growing states. The ever-growing collection of fully sequenced bacteriophages raises more questions than they answer, including the unknown functions of vast numbers of genes with no known homologs and of unknown function. Recombineering of phage genomes is central to addressing these questions, enabling the simple construction of mutants, determination of gene essentiality, and elucidation of gene function. In turn, advances in our understanding of phage genomics should present similar recombineering tools for dissecting a multitude of other genetically naïve bacterial systems.Fil: Marinelli, Laura J.. University of California; Estados UnidosFil: Hatfull, Graham F.. University of Pittsburgh; Estados UnidosFil: Piuri, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaLandes Bioscience2012-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/268961Marinelli, Laura J.; Hatfull, Graham F.; Piuri, Mariana; Recombineering: A powerful tool for modification of bacteriophage genomes.; Landes Bioscience; Bacteriophage; 2; 1; 1-2012; 5-142159-70732159-7081CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/10.4161/bact.18778info:eu-repo/semantics/altIdentifier/doi/10.4161/bact.18778info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:07:49Zoai:ri.conicet.gov.ar:11336/268961instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:07:49.87CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Recombineering: A powerful tool for modification of bacteriophage genomes. |
title |
Recombineering: A powerful tool for modification of bacteriophage genomes. |
spellingShingle |
Recombineering: A powerful tool for modification of bacteriophage genomes. Marinelli, Laura J. BACTERIOPHAGE RECOMBINEERING BRED MUTANT |
title_short |
Recombineering: A powerful tool for modification of bacteriophage genomes. |
title_full |
Recombineering: A powerful tool for modification of bacteriophage genomes. |
title_fullStr |
Recombineering: A powerful tool for modification of bacteriophage genomes. |
title_full_unstemmed |
Recombineering: A powerful tool for modification of bacteriophage genomes. |
title_sort |
Recombineering: A powerful tool for modification of bacteriophage genomes. |
dc.creator.none.fl_str_mv |
Marinelli, Laura J. Hatfull, Graham F. Piuri, Mariana |
author |
Marinelli, Laura J. |
author_facet |
Marinelli, Laura J. Hatfull, Graham F. Piuri, Mariana |
author_role |
author |
author2 |
Hatfull, Graham F. Piuri, Mariana |
author2_role |
author author |
dc.subject.none.fl_str_mv |
BACTERIOPHAGE RECOMBINEERING BRED MUTANT |
topic |
BACTERIOPHAGE RECOMBINEERING BRED MUTANT |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Recombineering, a recently developed technique for efficient genetic manipulation of bacteria, is facilitated by phagederived recombination proteins and has the advantage of using DNA substrates with short regions of homology. This system was first developed in Escherichia coli but has since been adapted for use in other bacteria. It is now widely used in a number of different systems for a variety of purposes, and the construction of chromosomal gene knockouts, deletions, insertions, point mutations, as well as in vivo cloning, mutagenesis of bacterial artificial chromosomes and phasmids, and the construction of genomic libraries has been reported. However, these methods also can be effectively applied to the genetic modification of bacteriophage genomes, in both their prophage and lytically growing states. The ever-growing collection of fully sequenced bacteriophages raises more questions than they answer, including the unknown functions of vast numbers of genes with no known homologs and of unknown function. Recombineering of phage genomes is central to addressing these questions, enabling the simple construction of mutants, determination of gene essentiality, and elucidation of gene function. In turn, advances in our understanding of phage genomics should present similar recombineering tools for dissecting a multitude of other genetically naïve bacterial systems. Fil: Marinelli, Laura J.. University of California; Estados Unidos Fil: Hatfull, Graham F.. University of Pittsburgh; Estados Unidos Fil: Piuri, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina |
description |
Recombineering, a recently developed technique for efficient genetic manipulation of bacteria, is facilitated by phagederived recombination proteins and has the advantage of using DNA substrates with short regions of homology. This system was first developed in Escherichia coli but has since been adapted for use in other bacteria. It is now widely used in a number of different systems for a variety of purposes, and the construction of chromosomal gene knockouts, deletions, insertions, point mutations, as well as in vivo cloning, mutagenesis of bacterial artificial chromosomes and phasmids, and the construction of genomic libraries has been reported. However, these methods also can be effectively applied to the genetic modification of bacteriophage genomes, in both their prophage and lytically growing states. The ever-growing collection of fully sequenced bacteriophages raises more questions than they answer, including the unknown functions of vast numbers of genes with no known homologs and of unknown function. Recombineering of phage genomes is central to addressing these questions, enabling the simple construction of mutants, determination of gene essentiality, and elucidation of gene function. In turn, advances in our understanding of phage genomics should present similar recombineering tools for dissecting a multitude of other genetically naïve bacterial systems. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-01 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/268961 Marinelli, Laura J.; Hatfull, Graham F.; Piuri, Mariana; Recombineering: A powerful tool for modification of bacteriophage genomes.; Landes Bioscience; Bacteriophage; 2; 1; 1-2012; 5-14 2159-7073 2159-7081 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/268961 |
identifier_str_mv |
Marinelli, Laura J.; Hatfull, Graham F.; Piuri, Mariana; Recombineering: A powerful tool for modification of bacteriophage genomes.; Landes Bioscience; Bacteriophage; 2; 1; 1-2012; 5-14 2159-7073 2159-7081 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/10.4161/bact.18778 info:eu-repo/semantics/altIdentifier/doi/10.4161/bact.18778 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Landes Bioscience |
publisher.none.fl_str_mv |
Landes Bioscience |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613942620454912 |
score |
13.070432 |