Detection and identification of Lactobacillus helveticus bacteriophages by PCR
- Autores
- Zago, Miriam; Rossetti, Lia; Reinheimer, Jorge Alberto; Carminati, Domenico; Giraffa, Giorgio
- Año de publicación
- 2008
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- A PCR protocol for detection of Lactobacillus helveticus bacteriophages was optimized. PCR was designed taking into account the sequence of the lys gene of temperate bacteriophage W-0303 and optimized to obtain a fragment of 222 bp using different Lb. helveticus phages from our collection. PCR was applied to total phage DNA extracted from 53 natural whey starters used for the production of Grana cheese and all gave the expected fragment. The presence of actively growing phages in the cultures was verified by traditional tests. Several PCR products of the lys gene were sequenced and aligned. The resulting sequences showed variable heterogeneity between the phages.
Fil: Zago, Miriam. Centro di Ricerca per le Produzioni Foraggere e Lattiero Casearie; Italia
Fil: Rossetti, Lia. Centro di Ricerca per le Produzioni Foraggere e Lattiero Casearie; Italia
Fil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Carminati, Domenico. Centro di Ricerca per le Produzioni Foraggere e Lattiero Casearie; Italia
Fil: Giraffa, Giorgio. Centro di Ricerca per le Produzioni Foraggere e Lattiero Casearie; Italia - Materia
-
LACTOBACILLUS HELVETICUS
BACTERIOPHAGES - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/113863
Ver los metadatos del registro completo
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Detection and identification of Lactobacillus helveticus bacteriophages by PCRZago, MiriamRossetti, LiaReinheimer, Jorge AlbertoCarminati, DomenicoGiraffa, GiorgioLACTOBACILLUS HELVETICUSBACTERIOPHAGEShttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2A PCR protocol for detection of Lactobacillus helveticus bacteriophages was optimized. PCR was designed taking into account the sequence of the lys gene of temperate bacteriophage W-0303 and optimized to obtain a fragment of 222 bp using different Lb. helveticus phages from our collection. PCR was applied to total phage DNA extracted from 53 natural whey starters used for the production of Grana cheese and all gave the expected fragment. The presence of actively growing phages in the cultures was verified by traditional tests. Several PCR products of the lys gene were sequenced and aligned. The resulting sequences showed variable heterogeneity between the phages.Fil: Zago, Miriam. Centro di Ricerca per le Produzioni Foraggere e Lattiero Casearie; ItaliaFil: Rossetti, Lia. Centro di Ricerca per le Produzioni Foraggere e Lattiero Casearie; ItaliaFil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaFil: Carminati, Domenico. Centro di Ricerca per le Produzioni Foraggere e Lattiero Casearie; ItaliaFil: Giraffa, Giorgio. Centro di Ricerca per le Produzioni Foraggere e Lattiero Casearie; ItaliaCambridge University Press2008-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/113863Zago, Miriam; Rossetti, Lia; Reinheimer, Jorge Alberto; Carminati, Domenico; Giraffa, Giorgio; Detection and identification of Lactobacillus helveticus bacteriophages by PCR; Cambridge University Press; Journal of Dairy Research; 75; 2; 12-2008; 196-2010022-0299CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1017/S0022029908003208info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:44:32Zoai:ri.conicet.gov.ar:11336/113863instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:44:32.357CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Detection and identification of Lactobacillus helveticus bacteriophages by PCR |
title |
Detection and identification of Lactobacillus helveticus bacteriophages by PCR |
spellingShingle |
Detection and identification of Lactobacillus helveticus bacteriophages by PCR Zago, Miriam LACTOBACILLUS HELVETICUS BACTERIOPHAGES |
title_short |
Detection and identification of Lactobacillus helveticus bacteriophages by PCR |
title_full |
Detection and identification of Lactobacillus helveticus bacteriophages by PCR |
title_fullStr |
Detection and identification of Lactobacillus helveticus bacteriophages by PCR |
title_full_unstemmed |
Detection and identification of Lactobacillus helveticus bacteriophages by PCR |
title_sort |
Detection and identification of Lactobacillus helveticus bacteriophages by PCR |
dc.creator.none.fl_str_mv |
Zago, Miriam Rossetti, Lia Reinheimer, Jorge Alberto Carminati, Domenico Giraffa, Giorgio |
author |
Zago, Miriam |
author_facet |
Zago, Miriam Rossetti, Lia Reinheimer, Jorge Alberto Carminati, Domenico Giraffa, Giorgio |
author_role |
author |
author2 |
Rossetti, Lia Reinheimer, Jorge Alberto Carminati, Domenico Giraffa, Giorgio |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
LACTOBACILLUS HELVETICUS BACTERIOPHAGES |
topic |
LACTOBACILLUS HELVETICUS BACTERIOPHAGES |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.11 https://purl.org/becyt/ford/2 |
dc.description.none.fl_txt_mv |
A PCR protocol for detection of Lactobacillus helveticus bacteriophages was optimized. PCR was designed taking into account the sequence of the lys gene of temperate bacteriophage W-0303 and optimized to obtain a fragment of 222 bp using different Lb. helveticus phages from our collection. PCR was applied to total phage DNA extracted from 53 natural whey starters used for the production of Grana cheese and all gave the expected fragment. The presence of actively growing phages in the cultures was verified by traditional tests. Several PCR products of the lys gene were sequenced and aligned. The resulting sequences showed variable heterogeneity between the phages. Fil: Zago, Miriam. Centro di Ricerca per le Produzioni Foraggere e Lattiero Casearie; Italia Fil: Rossetti, Lia. Centro di Ricerca per le Produzioni Foraggere e Lattiero Casearie; Italia Fil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina Fil: Carminati, Domenico. Centro di Ricerca per le Produzioni Foraggere e Lattiero Casearie; Italia Fil: Giraffa, Giorgio. Centro di Ricerca per le Produzioni Foraggere e Lattiero Casearie; Italia |
description |
A PCR protocol for detection of Lactobacillus helveticus bacteriophages was optimized. PCR was designed taking into account the sequence of the lys gene of temperate bacteriophage W-0303 and optimized to obtain a fragment of 222 bp using different Lb. helveticus phages from our collection. PCR was applied to total phage DNA extracted from 53 natural whey starters used for the production of Grana cheese and all gave the expected fragment. The presence of actively growing phages in the cultures was verified by traditional tests. Several PCR products of the lys gene were sequenced and aligned. The resulting sequences showed variable heterogeneity between the phages. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-12 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/113863 Zago, Miriam; Rossetti, Lia; Reinheimer, Jorge Alberto; Carminati, Domenico; Giraffa, Giorgio; Detection and identification of Lactobacillus helveticus bacteriophages by PCR; Cambridge University Press; Journal of Dairy Research; 75; 2; 12-2008; 196-201 0022-0299 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/113863 |
identifier_str_mv |
Zago, Miriam; Rossetti, Lia; Reinheimer, Jorge Alberto; Carminati, Domenico; Giraffa, Giorgio; Detection and identification of Lactobacillus helveticus bacteriophages by PCR; Cambridge University Press; Journal of Dairy Research; 75; 2; 12-2008; 196-201 0022-0299 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1017/S0022029908003208 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Cambridge University Press |
publisher.none.fl_str_mv |
Cambridge University Press |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614483422478336 |
score |
13.070432 |