Detection and identification of Lactobacillus helveticus bacteriophages by PCR

Autores
Zago, Miriam; Rossetti, Lia; Reinheimer, Jorge Alberto; Carminati, Domenico; Giraffa, Giorgio
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A PCR protocol for detection of Lactobacillus helveticus bacteriophages was optimized. PCR was designed taking into account the sequence of the lys gene of temperate bacteriophage W-0303 and optimized to obtain a fragment of 222 bp using different Lb. helveticus phages from our collection. PCR was applied to total phage DNA extracted from 53 natural whey starters used for the production of Grana cheese and all gave the expected fragment. The presence of actively growing phages in the cultures was verified by traditional tests. Several PCR products of the lys gene were sequenced and aligned. The resulting sequences showed variable heterogeneity between the phages.
Fil: Zago, Miriam. Centro di Ricerca per le Produzioni Foraggere e Lattiero Casearie; Italia
Fil: Rossetti, Lia. Centro di Ricerca per le Produzioni Foraggere e Lattiero Casearie; Italia
Fil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Carminati, Domenico. Centro di Ricerca per le Produzioni Foraggere e Lattiero Casearie; Italia
Fil: Giraffa, Giorgio. Centro di Ricerca per le Produzioni Foraggere e Lattiero Casearie; Italia
Materia
LACTOBACILLUS HELVETICUS
BACTERIOPHAGES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/113863
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/113863
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Detection and identification of Lactobacillus helveticus bacteriophages by PCRZago, MiriamRossetti, LiaReinheimer, Jorge AlbertoCarminati, DomenicoGiraffa, GiorgioLACTOBACILLUS HELVETICUSBACTERIOPHAGEShttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2A PCR protocol for detection of Lactobacillus helveticus bacteriophages was optimized. PCR was designed taking into account the sequence of the lys gene of temperate bacteriophage W-0303 and optimized to obtain a fragment of 222 bp using different Lb. helveticus phages from our collection. PCR was applied to total phage DNA extracted from 53 natural whey starters used for the production of Grana cheese and all gave the expected fragment. The presence of actively growing phages in the cultures was verified by traditional tests. Several PCR products of the lys gene were sequenced and aligned. The resulting sequences showed variable heterogeneity between the phages.Fil: Zago, Miriam. Centro di Ricerca per le Produzioni Foraggere e Lattiero Casearie; ItaliaFil: Rossetti, Lia. Centro di Ricerca per le Produzioni Foraggere e Lattiero Casearie; ItaliaFil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaFil: Carminati, Domenico. Centro di Ricerca per le Produzioni Foraggere e Lattiero Casearie; ItaliaFil: Giraffa, Giorgio. Centro di Ricerca per le Produzioni Foraggere e Lattiero Casearie; ItaliaCambridge University Press2008-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/113863Zago, Miriam; Rossetti, Lia; Reinheimer, Jorge Alberto; Carminati, Domenico; Giraffa, Giorgio; Detection and identification of Lactobacillus helveticus bacteriophages by PCR; Cambridge University Press; Journal of Dairy Research; 75; 2; 12-2008; 196-2010022-0299CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1017/S0022029908003208info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:17:45Zoai:ri.conicet.gov.ar:11336/113863instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:17:45.89CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Detection and identification of Lactobacillus helveticus bacteriophages by PCR
title Detection and identification of Lactobacillus helveticus bacteriophages by PCR
spellingShingle Detection and identification of Lactobacillus helveticus bacteriophages by PCR
Zago, Miriam
LACTOBACILLUS HELVETICUS
BACTERIOPHAGES
title_short Detection and identification of Lactobacillus helveticus bacteriophages by PCR
title_full Detection and identification of Lactobacillus helveticus bacteriophages by PCR
title_fullStr Detection and identification of Lactobacillus helveticus bacteriophages by PCR
title_full_unstemmed Detection and identification of Lactobacillus helveticus bacteriophages by PCR
title_sort Detection and identification of Lactobacillus helveticus bacteriophages by PCR
dc.creator.none.fl_str_mv Zago, Miriam
Rossetti, Lia
Reinheimer, Jorge Alberto
Carminati, Domenico
Giraffa, Giorgio
author Zago, Miriam
author_facet Zago, Miriam
Rossetti, Lia
Reinheimer, Jorge Alberto
Carminati, Domenico
Giraffa, Giorgio
author_role author
author2 Rossetti, Lia
Reinheimer, Jorge Alberto
Carminati, Domenico
Giraffa, Giorgio
author2_role author
author
author
author
dc.subject.none.fl_str_mv LACTOBACILLUS HELVETICUS
BACTERIOPHAGES
topic LACTOBACILLUS HELVETICUS
BACTERIOPHAGES
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv A PCR protocol for detection of Lactobacillus helveticus bacteriophages was optimized. PCR was designed taking into account the sequence of the lys gene of temperate bacteriophage W-0303 and optimized to obtain a fragment of 222 bp using different Lb. helveticus phages from our collection. PCR was applied to total phage DNA extracted from 53 natural whey starters used for the production of Grana cheese and all gave the expected fragment. The presence of actively growing phages in the cultures was verified by traditional tests. Several PCR products of the lys gene were sequenced and aligned. The resulting sequences showed variable heterogeneity between the phages.
Fil: Zago, Miriam. Centro di Ricerca per le Produzioni Foraggere e Lattiero Casearie; Italia
Fil: Rossetti, Lia. Centro di Ricerca per le Produzioni Foraggere e Lattiero Casearie; Italia
Fil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Carminati, Domenico. Centro di Ricerca per le Produzioni Foraggere e Lattiero Casearie; Italia
Fil: Giraffa, Giorgio. Centro di Ricerca per le Produzioni Foraggere e Lattiero Casearie; Italia
description A PCR protocol for detection of Lactobacillus helveticus bacteriophages was optimized. PCR was designed taking into account the sequence of the lys gene of temperate bacteriophage W-0303 and optimized to obtain a fragment of 222 bp using different Lb. helveticus phages from our collection. PCR was applied to total phage DNA extracted from 53 natural whey starters used for the production of Grana cheese and all gave the expected fragment. The presence of actively growing phages in the cultures was verified by traditional tests. Several PCR products of the lys gene were sequenced and aligned. The resulting sequences showed variable heterogeneity between the phages.
publishDate 2008
dc.date.none.fl_str_mv 2008-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/113863
Zago, Miriam; Rossetti, Lia; Reinheimer, Jorge Alberto; Carminati, Domenico; Giraffa, Giorgio; Detection and identification of Lactobacillus helveticus bacteriophages by PCR; Cambridge University Press; Journal of Dairy Research; 75; 2; 12-2008; 196-201
0022-0299
CONICET Digital
CONICET
url http://hdl.handle.net/11336/113863
identifier_str_mv Zago, Miriam; Rossetti, Lia; Reinheimer, Jorge Alberto; Carminati, Domenico; Giraffa, Giorgio; Detection and identification of Lactobacillus helveticus bacteriophages by PCR; Cambridge University Press; Journal of Dairy Research; 75; 2; 12-2008; 196-201
0022-0299
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1017/S0022029908003208
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cambridge University Press
publisher.none.fl_str_mv Cambridge University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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