A melanoma cell line exposed to extremely low frequency magnetic fields: assessment of proliferation

Autores
Guerra, Maria Florencia; Lacoste, Maria Gabriela; Eliçabe, Ricardo Javier; Anzulovich, Ana Cecilia; Makinistian, Leonardo
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Extremely low frequency magnetic fields (ELF-MF) have been the axis of heated discussions for decades for their possibly causal link to childhood leukemia. However, the ELF-MF are interesting for the opposite reason: a possible therapeutic use. Indeed, there are several in vitro experiments reporting inhibition of cancer cell proliferation, and some in vivo studies also point in the same direction: significant reduction of tumour growth has been reported in mice with induced breast cancer tumors, sarcoma, and melanoma. In order to elucidate the effect of magnetic fields on the B-16 cell line (murine melanoma), we built two identical systems of coils of cubic geometry. Each one consisted of a triaxial system of 3 pairs of coils in an orthogonal arrange. Then we proceeded to perform three experiments (each repeated three times). In all of them cells were seeded in 96-well microplates (one ?control? and one ?exposed?) and cell viability was measured by the MTT assay at T = 72 h (beginning of exposure was considered time zero, T = 0 h). A negative control (or sham-exposure) was first conducted were both plates were subjected to the same field (static, vertical 50 µT, ?MFref?). In a second experiment one of the plates was exposed to a 50 Hz 100 µTpeak AC field plus MFref while the other one was kept at MFref. In the third experiment a gradient of the DC field was evaluated. No significant differences were found between both plates in any of the three experiments. In summary, the combinations of AC/DC magnetic fields that we tested, for the time we did (duration of exposure: 1 h) did not affect the viability in the B-16 cell line. After an extensive study of the literature, which shows that ELF-MF intensity and waveform, duration, intermittence, and cycling of exposure can affect not only proliferation but also other endpoints such as the expression of clock genes, we decided to perform experiments to assess if ELF-MF affect the circadian expression of BMAL1. These experiments are currently underway.
Fil: Guerra, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich". Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich"; Argentina
Fil: Lacoste, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Eliçabe, Ricardo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Anzulovich, Ana Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Makinistian, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich". Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich"; Argentina
XXXVI Reunión Científica Anual de la Sociedad de Biología de Cuyo
San Luis
Argentina
Sociedad de Biología de Cuyo
Materia
LOW FREQUENCY MAGNETIC FIELDS
MELANOMA
PROLIFERATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/199653
Acceder
id CONICETDig_2f3f223dd806c142ba7bdd3b19b5452c
oai_identifier_str oai:ri.conicet.gov.ar:11336/199653
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A melanoma cell line exposed to extremely low frequency magnetic fields: assessment of proliferationGuerra, Maria FlorenciaLacoste, Maria GabrielaEliçabe, Ricardo JavierAnzulovich, Ana CeciliaMakinistian, LeonardoLOW FREQUENCY MAGNETIC FIELDSMELANOMAPROLIFERATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Extremely low frequency magnetic fields (ELF-MF) have been the axis of heated discussions for decades for their possibly causal link to childhood leukemia. However, the ELF-MF are interesting for the opposite reason: a possible therapeutic use. Indeed, there are several in vitro experiments reporting inhibition of cancer cell proliferation, and some in vivo studies also point in the same direction: significant reduction of tumour growth has been reported in mice with induced breast cancer tumors, sarcoma, and melanoma. In order to elucidate the effect of magnetic fields on the B-16 cell line (murine melanoma), we built two identical systems of coils of cubic geometry. Each one consisted of a triaxial system of 3 pairs of coils in an orthogonal arrange. Then we proceeded to perform three experiments (each repeated three times). In all of them cells were seeded in 96-well microplates (one ?control? and one ?exposed?) and cell viability was measured by the MTT assay at T = 72 h (beginning of exposure was considered time zero, T = 0 h). A negative control (or sham-exposure) was first conducted were both plates were subjected to the same field (static, vertical 50 µT, ?MFref?). In a second experiment one of the plates was exposed to a 50 Hz 100 µTpeak AC field plus MFref while the other one was kept at MFref. In the third experiment a gradient of the DC field was evaluated. No significant differences were found between both plates in any of the three experiments. In summary, the combinations of AC/DC magnetic fields that we tested, for the time we did (duration of exposure: 1 h) did not affect the viability in the B-16 cell line. After an extensive study of the literature, which shows that ELF-MF intensity and waveform, duration, intermittence, and cycling of exposure can affect not only proliferation but also other endpoints such as the expression of clock genes, we decided to perform experiments to assess if ELF-MF affect the circadian expression of BMAL1. These experiments are currently underway.Fil: Guerra, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich". Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich"; ArgentinaFil: Lacoste, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Eliçabe, Ricardo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Anzulovich, Ana Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Makinistian, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich". Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich"; ArgentinaXXXVI Reunión Científica Anual de la Sociedad de Biología de CuyoSan LuisArgentinaSociedad de Biología de CuyoSociedad de Biología de Cuyo2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/199653A melanoma cell line exposed to extremely low frequency magnetic fields: assessment of proliferation; XXXVI Reunión Científica Anual de la Sociedad de Biología de Cuyo; San Luis; Argentina; 2019; 18-18CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://sbcuyo.org.ar/wp-content/uploads/2019/12/Libro-BIOCELL-2019.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:14:52Zoai:ri.conicet.gov.ar:11336/199653instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:14:52.787CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A melanoma cell line exposed to extremely low frequency magnetic fields: assessment of proliferation
title A melanoma cell line exposed to extremely low frequency magnetic fields: assessment of proliferation
spellingShingle A melanoma cell line exposed to extremely low frequency magnetic fields: assessment of proliferation
Guerra, Maria Florencia
LOW FREQUENCY MAGNETIC FIELDS
MELANOMA
PROLIFERATION
title_short A melanoma cell line exposed to extremely low frequency magnetic fields: assessment of proliferation
title_full A melanoma cell line exposed to extremely low frequency magnetic fields: assessment of proliferation
title_fullStr A melanoma cell line exposed to extremely low frequency magnetic fields: assessment of proliferation
title_full_unstemmed A melanoma cell line exposed to extremely low frequency magnetic fields: assessment of proliferation
title_sort A melanoma cell line exposed to extremely low frequency magnetic fields: assessment of proliferation
dc.creator.none.fl_str_mv Guerra, Maria Florencia
Lacoste, Maria Gabriela
Eliçabe, Ricardo Javier
Anzulovich, Ana Cecilia
Makinistian, Leonardo
author Guerra, Maria Florencia
author_facet Guerra, Maria Florencia
Lacoste, Maria Gabriela
Eliçabe, Ricardo Javier
Anzulovich, Ana Cecilia
Makinistian, Leonardo
author_role author
author2 Lacoste, Maria Gabriela
Eliçabe, Ricardo Javier
Anzulovich, Ana Cecilia
Makinistian, Leonardo
author2_role author
author
author
author
dc.subject.none.fl_str_mv LOW FREQUENCY MAGNETIC FIELDS
MELANOMA
PROLIFERATION
topic LOW FREQUENCY MAGNETIC FIELDS
MELANOMA
PROLIFERATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Extremely low frequency magnetic fields (ELF-MF) have been the axis of heated discussions for decades for their possibly causal link to childhood leukemia. However, the ELF-MF are interesting for the opposite reason: a possible therapeutic use. Indeed, there are several in vitro experiments reporting inhibition of cancer cell proliferation, and some in vivo studies also point in the same direction: significant reduction of tumour growth has been reported in mice with induced breast cancer tumors, sarcoma, and melanoma. In order to elucidate the effect of magnetic fields on the B-16 cell line (murine melanoma), we built two identical systems of coils of cubic geometry. Each one consisted of a triaxial system of 3 pairs of coils in an orthogonal arrange. Then we proceeded to perform three experiments (each repeated three times). In all of them cells were seeded in 96-well microplates (one ?control? and one ?exposed?) and cell viability was measured by the MTT assay at T = 72 h (beginning of exposure was considered time zero, T = 0 h). A negative control (or sham-exposure) was first conducted were both plates were subjected to the same field (static, vertical 50 µT, ?MFref?). In a second experiment one of the plates was exposed to a 50 Hz 100 µTpeak AC field plus MFref while the other one was kept at MFref. In the third experiment a gradient of the DC field was evaluated. No significant differences were found between both plates in any of the three experiments. In summary, the combinations of AC/DC magnetic fields that we tested, for the time we did (duration of exposure: 1 h) did not affect the viability in the B-16 cell line. After an extensive study of the literature, which shows that ELF-MF intensity and waveform, duration, intermittence, and cycling of exposure can affect not only proliferation but also other endpoints such as the expression of clock genes, we decided to perform experiments to assess if ELF-MF affect the circadian expression of BMAL1. These experiments are currently underway.
Fil: Guerra, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich". Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich"; Argentina
Fil: Lacoste, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Eliçabe, Ricardo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Anzulovich, Ana Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Makinistian, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich". Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich"; Argentina
XXXVI Reunión Científica Anual de la Sociedad de Biología de Cuyo
San Luis
Argentina
Sociedad de Biología de Cuyo
description Extremely low frequency magnetic fields (ELF-MF) have been the axis of heated discussions for decades for their possibly causal link to childhood leukemia. However, the ELF-MF are interesting for the opposite reason: a possible therapeutic use. Indeed, there are several in vitro experiments reporting inhibition of cancer cell proliferation, and some in vivo studies also point in the same direction: significant reduction of tumour growth has been reported in mice with induced breast cancer tumors, sarcoma, and melanoma. In order to elucidate the effect of magnetic fields on the B-16 cell line (murine melanoma), we built two identical systems of coils of cubic geometry. Each one consisted of a triaxial system of 3 pairs of coils in an orthogonal arrange. Then we proceeded to perform three experiments (each repeated three times). In all of them cells were seeded in 96-well microplates (one ?control? and one ?exposed?) and cell viability was measured by the MTT assay at T = 72 h (beginning of exposure was considered time zero, T = 0 h). A negative control (or sham-exposure) was first conducted were both plates were subjected to the same field (static, vertical 50 µT, ?MFref?). In a second experiment one of the plates was exposed to a 50 Hz 100 µTpeak AC field plus MFref while the other one was kept at MFref. In the third experiment a gradient of the DC field was evaluated. No significant differences were found between both plates in any of the three experiments. In summary, the combinations of AC/DC magnetic fields that we tested, for the time we did (duration of exposure: 1 h) did not affect the viability in the B-16 cell line. After an extensive study of the literature, which shows that ELF-MF intensity and waveform, duration, intermittence, and cycling of exposure can affect not only proliferation but also other endpoints such as the expression of clock genes, we decided to perform experiments to assess if ELF-MF affect the circadian expression of BMAL1. These experiments are currently underway.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/199653
A melanoma cell line exposed to extremely low frequency magnetic fields: assessment of proliferation; XXXVI Reunión Científica Anual de la Sociedad de Biología de Cuyo; San Luis; Argentina; 2019; 18-18
CONICET Digital
CONICET
url http://hdl.handle.net/11336/199653
identifier_str_mv A melanoma cell line exposed to extremely low frequency magnetic fields: assessment of proliferation; XXXVI Reunión Científica Anual de la Sociedad de Biología de Cuyo; San Luis; Argentina; 2019; 18-18
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://sbcuyo.org.ar/wp-content/uploads/2019/12/Libro-BIOCELL-2019.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Sociedad de Biología de Cuyo
publisher.none.fl_str_mv Sociedad de Biología de Cuyo
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846782559386599424
score 12.982451

Publicaciones similares