A melanoma cell line exposed to extremely low frequency magnetic fields: assessment of proliferation
- Autores
- Guerra, Maria Florencia; Lacoste, Maria Gabriela; Eliçabe, Ricardo Javier; Anzulovich, Ana Cecilia; Makinistian, Leonardo
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- Extremely low frequency magnetic fields (ELF-MF) have been the axis of heated discussions for decades for their possibly causal link to childhood leukemia. However, the ELF-MF are interesting for the opposite reason: a possible therapeutic use. Indeed, there are several in vitro experiments reporting inhibition of cancer cell proliferation, and some in vivo studies also point in the same direction: significant reduction of tumour growth has been reported in mice with induced breast cancer tumors, sarcoma, and melanoma. In order to elucidate the effect of magnetic fields on the B-16 cell line (murine melanoma), we built two identical systems of coils of cubic geometry. Each one consisted of a triaxial system of 3 pairs of coils in an orthogonal arrange. Then we proceeded to perform three experiments (each repeated three times). In all of them cells were seeded in 96-well microplates (one ?control? and one ?exposed?) and cell viability was measured by the MTT assay at T = 72 h (beginning of exposure was considered time zero, T = 0 h). A negative control (or sham-exposure) was first conducted were both plates were subjected to the same field (static, vertical 50 µT, ?MFref?). In a second experiment one of the plates was exposed to a 50 Hz 100 µTpeak AC field plus MFref while the other one was kept at MFref. In the third experiment a gradient of the DC field was evaluated. No significant differences were found between both plates in any of the three experiments. In summary, the combinations of AC/DC magnetic fields that we tested, for the time we did (duration of exposure: 1 h) did not affect the viability in the B-16 cell line. After an extensive study of the literature, which shows that ELF-MF intensity and waveform, duration, intermittence, and cycling of exposure can affect not only proliferation but also other endpoints such as the expression of clock genes, we decided to perform experiments to assess if ELF-MF affect the circadian expression of BMAL1. These experiments are currently underway.
Fil: Guerra, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich". Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich"; Argentina
Fil: Lacoste, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Eliçabe, Ricardo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Anzulovich, Ana Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Makinistian, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich". Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich"; Argentina
XXXVI Reunión Científica Anual de la Sociedad de Biología de Cuyo
San Luis
Argentina
Sociedad de Biología de Cuyo - Materia
-
LOW FREQUENCY MAGNETIC FIELDS
MELANOMA
PROLIFERATION - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/199653
Ver los metadatos del registro completo
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A melanoma cell line exposed to extremely low frequency magnetic fields: assessment of proliferationGuerra, Maria FlorenciaLacoste, Maria GabrielaEliçabe, Ricardo JavierAnzulovich, Ana CeciliaMakinistian, LeonardoLOW FREQUENCY MAGNETIC FIELDSMELANOMAPROLIFERATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Extremely low frequency magnetic fields (ELF-MF) have been the axis of heated discussions for decades for their possibly causal link to childhood leukemia. However, the ELF-MF are interesting for the opposite reason: a possible therapeutic use. Indeed, there are several in vitro experiments reporting inhibition of cancer cell proliferation, and some in vivo studies also point in the same direction: significant reduction of tumour growth has been reported in mice with induced breast cancer tumors, sarcoma, and melanoma. In order to elucidate the effect of magnetic fields on the B-16 cell line (murine melanoma), we built two identical systems of coils of cubic geometry. Each one consisted of a triaxial system of 3 pairs of coils in an orthogonal arrange. Then we proceeded to perform three experiments (each repeated three times). In all of them cells were seeded in 96-well microplates (one ?control? and one ?exposed?) and cell viability was measured by the MTT assay at T = 72 h (beginning of exposure was considered time zero, T = 0 h). A negative control (or sham-exposure) was first conducted were both plates were subjected to the same field (static, vertical 50 µT, ?MFref?). In a second experiment one of the plates was exposed to a 50 Hz 100 µTpeak AC field plus MFref while the other one was kept at MFref. In the third experiment a gradient of the DC field was evaluated. No significant differences were found between both plates in any of the three experiments. In summary, the combinations of AC/DC magnetic fields that we tested, for the time we did (duration of exposure: 1 h) did not affect the viability in the B-16 cell line. After an extensive study of the literature, which shows that ELF-MF intensity and waveform, duration, intermittence, and cycling of exposure can affect not only proliferation but also other endpoints such as the expression of clock genes, we decided to perform experiments to assess if ELF-MF affect the circadian expression of BMAL1. These experiments are currently underway.Fil: Guerra, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich". Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich"; ArgentinaFil: Lacoste, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Eliçabe, Ricardo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Anzulovich, Ana Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Makinistian, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich". Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich"; ArgentinaXXXVI Reunión Científica Anual de la Sociedad de Biología de CuyoSan LuisArgentinaSociedad de Biología de CuyoSociedad de Biología de Cuyo2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/199653A melanoma cell line exposed to extremely low frequency magnetic fields: assessment of proliferation; XXXVI Reunión Científica Anual de la Sociedad de Biología de Cuyo; San Luis; Argentina; 2019; 18-18CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://sbcuyo.org.ar/wp-content/uploads/2019/12/Libro-BIOCELL-2019.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:42:12Zoai:ri.conicet.gov.ar:11336/199653instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:42:12.522CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
A melanoma cell line exposed to extremely low frequency magnetic fields: assessment of proliferation |
title |
A melanoma cell line exposed to extremely low frequency magnetic fields: assessment of proliferation |
spellingShingle |
A melanoma cell line exposed to extremely low frequency magnetic fields: assessment of proliferation Guerra, Maria Florencia LOW FREQUENCY MAGNETIC FIELDS MELANOMA PROLIFERATION |
title_short |
A melanoma cell line exposed to extremely low frequency magnetic fields: assessment of proliferation |
title_full |
A melanoma cell line exposed to extremely low frequency magnetic fields: assessment of proliferation |
title_fullStr |
A melanoma cell line exposed to extremely low frequency magnetic fields: assessment of proliferation |
title_full_unstemmed |
A melanoma cell line exposed to extremely low frequency magnetic fields: assessment of proliferation |
title_sort |
A melanoma cell line exposed to extremely low frequency magnetic fields: assessment of proliferation |
dc.creator.none.fl_str_mv |
Guerra, Maria Florencia Lacoste, Maria Gabriela Eliçabe, Ricardo Javier Anzulovich, Ana Cecilia Makinistian, Leonardo |
author |
Guerra, Maria Florencia |
author_facet |
Guerra, Maria Florencia Lacoste, Maria Gabriela Eliçabe, Ricardo Javier Anzulovich, Ana Cecilia Makinistian, Leonardo |
author_role |
author |
author2 |
Lacoste, Maria Gabriela Eliçabe, Ricardo Javier Anzulovich, Ana Cecilia Makinistian, Leonardo |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
LOW FREQUENCY MAGNETIC FIELDS MELANOMA PROLIFERATION |
topic |
LOW FREQUENCY MAGNETIC FIELDS MELANOMA PROLIFERATION |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Extremely low frequency magnetic fields (ELF-MF) have been the axis of heated discussions for decades for their possibly causal link to childhood leukemia. However, the ELF-MF are interesting for the opposite reason: a possible therapeutic use. Indeed, there are several in vitro experiments reporting inhibition of cancer cell proliferation, and some in vivo studies also point in the same direction: significant reduction of tumour growth has been reported in mice with induced breast cancer tumors, sarcoma, and melanoma. In order to elucidate the effect of magnetic fields on the B-16 cell line (murine melanoma), we built two identical systems of coils of cubic geometry. Each one consisted of a triaxial system of 3 pairs of coils in an orthogonal arrange. Then we proceeded to perform three experiments (each repeated three times). In all of them cells were seeded in 96-well microplates (one ?control? and one ?exposed?) and cell viability was measured by the MTT assay at T = 72 h (beginning of exposure was considered time zero, T = 0 h). A negative control (or sham-exposure) was first conducted were both plates were subjected to the same field (static, vertical 50 µT, ?MFref?). In a second experiment one of the plates was exposed to a 50 Hz 100 µTpeak AC field plus MFref while the other one was kept at MFref. In the third experiment a gradient of the DC field was evaluated. No significant differences were found between both plates in any of the three experiments. In summary, the combinations of AC/DC magnetic fields that we tested, for the time we did (duration of exposure: 1 h) did not affect the viability in the B-16 cell line. After an extensive study of the literature, which shows that ELF-MF intensity and waveform, duration, intermittence, and cycling of exposure can affect not only proliferation but also other endpoints such as the expression of clock genes, we decided to perform experiments to assess if ELF-MF affect the circadian expression of BMAL1. These experiments are currently underway. Fil: Guerra, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich". Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich"; Argentina Fil: Lacoste, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina Fil: Eliçabe, Ricardo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina Fil: Anzulovich, Ana Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina Fil: Makinistian, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich". Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich"; Argentina XXXVI Reunión Científica Anual de la Sociedad de Biología de Cuyo San Luis Argentina Sociedad de Biología de Cuyo |
description |
Extremely low frequency magnetic fields (ELF-MF) have been the axis of heated discussions for decades for their possibly causal link to childhood leukemia. However, the ELF-MF are interesting for the opposite reason: a possible therapeutic use. Indeed, there are several in vitro experiments reporting inhibition of cancer cell proliferation, and some in vivo studies also point in the same direction: significant reduction of tumour growth has been reported in mice with induced breast cancer tumors, sarcoma, and melanoma. In order to elucidate the effect of magnetic fields on the B-16 cell line (murine melanoma), we built two identical systems of coils of cubic geometry. Each one consisted of a triaxial system of 3 pairs of coils in an orthogonal arrange. Then we proceeded to perform three experiments (each repeated three times). In all of them cells were seeded in 96-well microplates (one ?control? and one ?exposed?) and cell viability was measured by the MTT assay at T = 72 h (beginning of exposure was considered time zero, T = 0 h). A negative control (or sham-exposure) was first conducted were both plates were subjected to the same field (static, vertical 50 µT, ?MFref?). In a second experiment one of the plates was exposed to a 50 Hz 100 µTpeak AC field plus MFref while the other one was kept at MFref. In the third experiment a gradient of the DC field was evaluated. No significant differences were found between both plates in any of the three experiments. In summary, the combinations of AC/DC magnetic fields that we tested, for the time we did (duration of exposure: 1 h) did not affect the viability in the B-16 cell line. After an extensive study of the literature, which shows that ELF-MF intensity and waveform, duration, intermittence, and cycling of exposure can affect not only proliferation but also other endpoints such as the expression of clock genes, we decided to perform experiments to assess if ELF-MF affect the circadian expression of BMAL1. These experiments are currently underway. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Congreso Book http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
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publishedVersion |
format |
conferenceObject |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/199653 A melanoma cell line exposed to extremely low frequency magnetic fields: assessment of proliferation; XXXVI Reunión Científica Anual de la Sociedad de Biología de Cuyo; San Luis; Argentina; 2019; 18-18 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/199653 |
identifier_str_mv |
A melanoma cell line exposed to extremely low frequency magnetic fields: assessment of proliferation; XXXVI Reunión Científica Anual de la Sociedad de Biología de Cuyo; San Luis; Argentina; 2019; 18-18 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
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info:eu-repo/semantics/altIdentifier/url/https://sbcuyo.org.ar/wp-content/uploads/2019/12/Libro-BIOCELL-2019.pdf |
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info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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Nacional |
dc.publisher.none.fl_str_mv |
Sociedad de Biología de Cuyo |
publisher.none.fl_str_mv |
Sociedad de Biología de Cuyo |
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reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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