A high throughput screening system of coils for ELF magnetic fields experiments: Proof of concept on the proliferation of cancer cell lines

• Autores: Makinistian, Leonardo; Marková, Eva; Belyaev, Igor
• Año de publicación: 2019
• Idioma: inglés
• Tipo de recurso: artículo
• Materia: CANCER CELL LINE PROLIFERATION; ELF MAGNETIC FIELDS; HIGH THROUGHPUT SCREENING; MDA-MB-231; U251
• Institución: Consejo Nacional de Investigaciones Científicas y Técnicas

Background: It has been demonstrated that relatively small variations of the parameters of exposure to extremely low frequency magnetic fields (ELF-MF) can change significantly the outcome of experiments. Hence, either in trying to elucidate if these fields are carcinogenic, or in exploring their possible therapeutic use, it is desirable to screen through as many different exposures as possible. The purpose of this work is to provide a proof of concept of how a recently reported system of coils allows testing different field exposures, in a single experiment. Methods: Using a novel exposure system, we subjected a glioblastoma cancer cell line (U251) to three different time modulations of an ELF-MF at 60 different combinations of the alternated current (AC) and direct current (DC) components of the field. One of those three time modulations was also tested on another cell line, MDA-MB-231 (breast cancer). After exposure, proliferation was assessed by colorimetric assays. Results: For the U251 cells, a total of 180 different exposures were tested in three different experiments. Depending on exposure modulation and AC field intensity (but, remarkably, not on DC intensity), we found the three possible outcomes: increase (14.3% above control, p < 0.01), decrease (16.6% below control, p < 0.001), and also no-effect on proliferation with respect to control. Only the time modulation that inhibited proliferation of U251 was also tested on MDA-MB-231 cells which, in contrast, showed no alteration of their proliferation on any of the 60 AC/DC field combinations tested. Conclusions: We demonstrated, for the first time, the use of a novel system of coils for magnetobiology research, which allowed us to find that differences of only a few μT resulted in statistically different results. Not only does our study demonstrate the relevance of the time modulation and the importance of finely sweeping through the AC and DC amplitudes, but also, and most importantly, provides a proof of concept of a system that sensibly reduces the time and costs of screening.
Fil: Makinistian, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich". Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich"; Argentina. Slovak Academy of Sciences. Biomedical Center. Cancer Research Institute. Department of Radiobiology; Eslovaquia
Fil: Marková, Eva. Slovak Academy of Sciences. Biomedical Center. Cancer Research Institute. Department of Radiobiology; Eslovaquia
Fil: Belyaev, Igor. Slovak Academy of Sciences. Biomedical Center. Cancer Research Institute. Department of Radiobiology; Eslovaquia