Preliminary Study on Artificial versus Animal-Based Feeding Systems for Amblyomma Ticks (Acari: Ixodidae)

Autores
Sebastian, Patrick Stephan; Król, Nina; Novoa, María Belén; Nijhof, Ard Menzo; Pfeffer, Martin; Nava, Santiago; Obiegala, Anna
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Hard ticks pose a threat to animal and human health. Active life stages need to feed on a vertebrate host in order to complete their life cycle. To study processes such as tick-pathogen interactions or drug efficacy and pharmacokinetics, it is necessary to maintain tick colonies under defined laboratory conditions, typically using laboratory animals. The aim of this study was to test a membrane-based artificial feeding system (AFS) applicable for Amblyomma ticks using Amblyomma tonelliae as a biological model. Adult ticks from a laboratory colony were fed in a membrane-based AFS. For comparison, other A. tonelliae adults were fed on calf and rabbit. The proportions of attached (AFS: 76%; calf/rabbit: 100%) and engorged females (AFS: 47.4%; calf/rabbit: 100%) in the AFS were significantly lower compared to animal-based feeding (p = 0.0265). The engorgement weight of in vitro fed ticks ((Formula presented.) = 658 mg; SD ± 259.80) did not significantly differ from that of ticks fed on animals (p = 0.3272, respectively 0.0947). The proportion of females that oviposited was 100% for all three feeding methods. However, the incubation period of eggs ((Formula presented.) = 54 days; SD ± 7) was longer in the AFS compared to conventional animal-based feeding (p = 0.0014); (Formula presented.) = 45 days; SD ± 2 in the rabbit and (p = 0.0144). (Formula presented.) = 48 days; SD ± 2 in the calf). Egg cluster hatching ((Formula presented.) = 41%; SD ± 44.82) was lower in the AFS than in the other feeding methods (rabbit: (Formula presented.) = 74%; SD ± 20; p = 0.0529; calf: (Formula presented.) = 81%; SD ± 22; p = 0.0256). Although the attachment, development, and the hatching of AFS ticks were below those from animal-based feeding, the method may be useful in future experiments. Nevertheless, further experiments with a higher number of tick specimens (including immature life stages) and different attractant stimuli are required to confirm the preliminary results of this study and to evaluate the applicability of AFS for Amblyomma ticks as an alternative to animal-based feeding methods.
Fil: Sebastian, Patrick Stephan. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; Argentina
Fil: Król, Nina. Universitat Leipzig; Alemania
Fil: Novoa, María Belén. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; Argentina
Fil: Nijhof, Ard Menzo. Freie Universität Berlin; Alemania
Fil: Pfeffer, Martin. Universitat Leipzig; Alemania
Fil: Nava, Santiago. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; Argentina
Fil: Obiegala, Anna. Universitat Leipzig; Alemania
Materia
3R PRINCIPLE
ARGENTINA
FEEDING SYSTEMS
HARD TICKS
LABORATORY ANIMALS
MEMBRANE-BASED FEEDING
SOUTH AMERICA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/225715
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/225715
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Preliminary Study on Artificial versus Animal-Based Feeding Systems for Amblyomma Ticks (Acari: Ixodidae)Sebastian, Patrick StephanKról, NinaNovoa, María BelénNijhof, Ard MenzoPfeffer, MartinNava, SantiagoObiegala, Anna3R PRINCIPLEARGENTINAFEEDING SYSTEMSHARD TICKSLABORATORY ANIMALSMEMBRANE-BASED FEEDINGSOUTH AMERICAhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Hard ticks pose a threat to animal and human health. Active life stages need to feed on a vertebrate host in order to complete their life cycle. To study processes such as tick-pathogen interactions or drug efficacy and pharmacokinetics, it is necessary to maintain tick colonies under defined laboratory conditions, typically using laboratory animals. The aim of this study was to test a membrane-based artificial feeding system (AFS) applicable for Amblyomma ticks using Amblyomma tonelliae as a biological model. Adult ticks from a laboratory colony were fed in a membrane-based AFS. For comparison, other A. tonelliae adults were fed on calf and rabbit. The proportions of attached (AFS: 76%; calf/rabbit: 100%) and engorged females (AFS: 47.4%; calf/rabbit: 100%) in the AFS were significantly lower compared to animal-based feeding (p = 0.0265). The engorgement weight of in vitro fed ticks ((Formula presented.) = 658 mg; SD ± 259.80) did not significantly differ from that of ticks fed on animals (p = 0.3272, respectively 0.0947). The proportion of females that oviposited was 100% for all three feeding methods. However, the incubation period of eggs ((Formula presented.) = 54 days; SD ± 7) was longer in the AFS compared to conventional animal-based feeding (p = 0.0014); (Formula presented.) = 45 days; SD ± 2 in the rabbit and (p = 0.0144). (Formula presented.) = 48 days; SD ± 2 in the calf). Egg cluster hatching ((Formula presented.) = 41%; SD ± 44.82) was lower in the AFS than in the other feeding methods (rabbit: (Formula presented.) = 74%; SD ± 20; p = 0.0529; calf: (Formula presented.) = 81%; SD ± 22; p = 0.0256). Although the attachment, development, and the hatching of AFS ticks were below those from animal-based feeding, the method may be useful in future experiments. Nevertheless, further experiments with a higher number of tick specimens (including immature life stages) and different attractant stimuli are required to confirm the preliminary results of this study and to evaluate the applicability of AFS for Amblyomma ticks as an alternative to animal-based feeding methods.Fil: Sebastian, Patrick Stephan. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; ArgentinaFil: Król, Nina. Universitat Leipzig; AlemaniaFil: Novoa, María Belén. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; ArgentinaFil: Nijhof, Ard Menzo. Freie Universität Berlin; AlemaniaFil: Pfeffer, Martin. Universitat Leipzig; AlemaniaFil: Nava, Santiago. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; ArgentinaFil: Obiegala, Anna. Universitat Leipzig; AlemaniaMDPI2023-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/225715Sebastian, Patrick Stephan; Król, Nina; Novoa, María Belén; Nijhof, Ard Menzo; Pfeffer, Martin; et al.; Preliminary Study on Artificial versus Animal-Based Feeding Systems for Amblyomma Ticks (Acari: Ixodidae); MDPI; Microorganisms; 11; 5; 4-2023; 1-102076-2607CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-2607/11/5/1107info:eu-repo/semantics/altIdentifier/doi/10.3390/microorganisms11051107info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:48:45Zoai:ri.conicet.gov.ar:11336/225715instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:48:45.376CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Preliminary Study on Artificial versus Animal-Based Feeding Systems for Amblyomma Ticks (Acari: Ixodidae)
title Preliminary Study on Artificial versus Animal-Based Feeding Systems for Amblyomma Ticks (Acari: Ixodidae)
spellingShingle Preliminary Study on Artificial versus Animal-Based Feeding Systems for Amblyomma Ticks (Acari: Ixodidae)
Sebastian, Patrick Stephan
3R PRINCIPLE
ARGENTINA
FEEDING SYSTEMS
HARD TICKS
LABORATORY ANIMALS
MEMBRANE-BASED FEEDING
SOUTH AMERICA
title_short Preliminary Study on Artificial versus Animal-Based Feeding Systems for Amblyomma Ticks (Acari: Ixodidae)
title_full Preliminary Study on Artificial versus Animal-Based Feeding Systems for Amblyomma Ticks (Acari: Ixodidae)
title_fullStr Preliminary Study on Artificial versus Animal-Based Feeding Systems for Amblyomma Ticks (Acari: Ixodidae)
title_full_unstemmed Preliminary Study on Artificial versus Animal-Based Feeding Systems for Amblyomma Ticks (Acari: Ixodidae)
title_sort Preliminary Study on Artificial versus Animal-Based Feeding Systems for Amblyomma Ticks (Acari: Ixodidae)
dc.creator.none.fl_str_mv Sebastian, Patrick Stephan
Król, Nina
Novoa, María Belén
Nijhof, Ard Menzo
Pfeffer, Martin
Nava, Santiago
Obiegala, Anna
author Sebastian, Patrick Stephan
author_facet Sebastian, Patrick Stephan
Król, Nina
Novoa, María Belén
Nijhof, Ard Menzo
Pfeffer, Martin
Nava, Santiago
Obiegala, Anna
author_role author
author2 Król, Nina
Novoa, María Belén
Nijhof, Ard Menzo
Pfeffer, Martin
Nava, Santiago
Obiegala, Anna
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv 3R PRINCIPLE
ARGENTINA
FEEDING SYSTEMS
HARD TICKS
LABORATORY ANIMALS
MEMBRANE-BASED FEEDING
SOUTH AMERICA
topic 3R PRINCIPLE
ARGENTINA
FEEDING SYSTEMS
HARD TICKS
LABORATORY ANIMALS
MEMBRANE-BASED FEEDING
SOUTH AMERICA
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Hard ticks pose a threat to animal and human health. Active life stages need to feed on a vertebrate host in order to complete their life cycle. To study processes such as tick-pathogen interactions or drug efficacy and pharmacokinetics, it is necessary to maintain tick colonies under defined laboratory conditions, typically using laboratory animals. The aim of this study was to test a membrane-based artificial feeding system (AFS) applicable for Amblyomma ticks using Amblyomma tonelliae as a biological model. Adult ticks from a laboratory colony were fed in a membrane-based AFS. For comparison, other A. tonelliae adults were fed on calf and rabbit. The proportions of attached (AFS: 76%; calf/rabbit: 100%) and engorged females (AFS: 47.4%; calf/rabbit: 100%) in the AFS were significantly lower compared to animal-based feeding (p = 0.0265). The engorgement weight of in vitro fed ticks ((Formula presented.) = 658 mg; SD ± 259.80) did not significantly differ from that of ticks fed on animals (p = 0.3272, respectively 0.0947). The proportion of females that oviposited was 100% for all three feeding methods. However, the incubation period of eggs ((Formula presented.) = 54 days; SD ± 7) was longer in the AFS compared to conventional animal-based feeding (p = 0.0014); (Formula presented.) = 45 days; SD ± 2 in the rabbit and (p = 0.0144). (Formula presented.) = 48 days; SD ± 2 in the calf). Egg cluster hatching ((Formula presented.) = 41%; SD ± 44.82) was lower in the AFS than in the other feeding methods (rabbit: (Formula presented.) = 74%; SD ± 20; p = 0.0529; calf: (Formula presented.) = 81%; SD ± 22; p = 0.0256). Although the attachment, development, and the hatching of AFS ticks were below those from animal-based feeding, the method may be useful in future experiments. Nevertheless, further experiments with a higher number of tick specimens (including immature life stages) and different attractant stimuli are required to confirm the preliminary results of this study and to evaluate the applicability of AFS for Amblyomma ticks as an alternative to animal-based feeding methods.
Fil: Sebastian, Patrick Stephan. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; Argentina
Fil: Król, Nina. Universitat Leipzig; Alemania
Fil: Novoa, María Belén. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; Argentina
Fil: Nijhof, Ard Menzo. Freie Universität Berlin; Alemania
Fil: Pfeffer, Martin. Universitat Leipzig; Alemania
Fil: Nava, Santiago. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; Argentina
Fil: Obiegala, Anna. Universitat Leipzig; Alemania
description Hard ticks pose a threat to animal and human health. Active life stages need to feed on a vertebrate host in order to complete their life cycle. To study processes such as tick-pathogen interactions or drug efficacy and pharmacokinetics, it is necessary to maintain tick colonies under defined laboratory conditions, typically using laboratory animals. The aim of this study was to test a membrane-based artificial feeding system (AFS) applicable for Amblyomma ticks using Amblyomma tonelliae as a biological model. Adult ticks from a laboratory colony were fed in a membrane-based AFS. For comparison, other A. tonelliae adults were fed on calf and rabbit. The proportions of attached (AFS: 76%; calf/rabbit: 100%) and engorged females (AFS: 47.4%; calf/rabbit: 100%) in the AFS were significantly lower compared to animal-based feeding (p = 0.0265). The engorgement weight of in vitro fed ticks ((Formula presented.) = 658 mg; SD ± 259.80) did not significantly differ from that of ticks fed on animals (p = 0.3272, respectively 0.0947). The proportion of females that oviposited was 100% for all three feeding methods. However, the incubation period of eggs ((Formula presented.) = 54 days; SD ± 7) was longer in the AFS compared to conventional animal-based feeding (p = 0.0014); (Formula presented.) = 45 days; SD ± 2 in the rabbit and (p = 0.0144). (Formula presented.) = 48 days; SD ± 2 in the calf). Egg cluster hatching ((Formula presented.) = 41%; SD ± 44.82) was lower in the AFS than in the other feeding methods (rabbit: (Formula presented.) = 74%; SD ± 20; p = 0.0529; calf: (Formula presented.) = 81%; SD ± 22; p = 0.0256). Although the attachment, development, and the hatching of AFS ticks were below those from animal-based feeding, the method may be useful in future experiments. Nevertheless, further experiments with a higher number of tick specimens (including immature life stages) and different attractant stimuli are required to confirm the preliminary results of this study and to evaluate the applicability of AFS for Amblyomma ticks as an alternative to animal-based feeding methods.
publishDate 2023
dc.date.none.fl_str_mv 2023-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/225715
Sebastian, Patrick Stephan; Król, Nina; Novoa, María Belén; Nijhof, Ard Menzo; Pfeffer, Martin; et al.; Preliminary Study on Artificial versus Animal-Based Feeding Systems for Amblyomma Ticks (Acari: Ixodidae); MDPI; Microorganisms; 11; 5; 4-2023; 1-10
2076-2607
CONICET Digital
CONICET
url http://hdl.handle.net/11336/225715
identifier_str_mv Sebastian, Patrick Stephan; Król, Nina; Novoa, María Belén; Nijhof, Ard Menzo; Pfeffer, Martin; et al.; Preliminary Study on Artificial versus Animal-Based Feeding Systems for Amblyomma Ticks (Acari: Ixodidae); MDPI; Microorganisms; 11; 5; 4-2023; 1-10
2076-2607
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-2607/11/5/1107
info:eu-repo/semantics/altIdentifier/doi/10.3390/microorganisms11051107
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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