Alterations of LXRα and LXRβ expression in the hypothalamus of glucose-intolerant rats
- Autores
- Kruse, Maria Sol; Rey, Mariana; Vega, Maria Cristina; Coirini, Hector
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Liver X receptor (LXR) a and b are nuclear receptors that are crucial for the regulation of carbohydrate and lipid metabolism. Activation of LXRs in the brain facilitates cholesterol clearance and improves cognitive deficits, thus they are considered as promising drug targets to treat diseases such as atherosclerosis and Alzheimer´s disease. Nevertheless, little is known about the function and localization of LXRs in the brain. Here, we studied the expression of LXR in the brains of rats that received free access to 10% (w/v) fructose group (FG) in their beverages or water control drinks (control group (CG)). After 6 weeks rats in the FG presented with hypertriglyceridemia, hyperinsulinemia, and became glucose intolerant, suggesting a progression toward type 2 diabetes. We found that hypothalamic LXR expression was altered in fructose-fed rats. Rats in the FG presented with a decrease in LXRb levels while showing an increase in LXRa expression in the hypothalamus but not in the hippocampus, cerebellum, or neocortex. Moreover, both LXRa and b expression correlated negatively with insulin and triglyceride levels. Interestingly, LXRb showed a negative correlation with the area under the curve during the glucose tolerance test in the CG and a positive correlation in the FG. Immunocytochemistry revealed that the paraventricular and ventromedial nuclei express mainly LXRa whereas the arcuate nucleus expresses LXRb. Both LXR immunosignals were found in the median preoptic area. This is the first study showing a relationship between glucose and lipid homeostasis and the expression of LXRs in the hypothalamus, suggesting that LXRs may trigger neurochemical and neurophysiological responses for the control of food intake and energy expenditure through these receptors.
Fil: Kruse, Maria Sol. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Rey, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Vega, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Coirini, Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; Argentina. Universidad Catolica de Cuyo - Sede San Juan; Argentina - Materia
-
Lxr Receptors
Glucose Intolerant
Hypothalamus - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/22184
Ver los metadatos del registro completo
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Alterations of LXRα and LXRβ expression in the hypothalamus of glucose-intolerant ratsKruse, Maria SolRey, MarianaVega, Maria CristinaCoirini, HectorLxr ReceptorsGlucose IntolerantHypothalamushttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Liver X receptor (LXR) a and b are nuclear receptors that are crucial for the regulation of carbohydrate and lipid metabolism. Activation of LXRs in the brain facilitates cholesterol clearance and improves cognitive deficits, thus they are considered as promising drug targets to treat diseases such as atherosclerosis and Alzheimer´s disease. Nevertheless, little is known about the function and localization of LXRs in the brain. Here, we studied the expression of LXR in the brains of rats that received free access to 10% (w/v) fructose group (FG) in their beverages or water control drinks (control group (CG)). After 6 weeks rats in the FG presented with hypertriglyceridemia, hyperinsulinemia, and became glucose intolerant, suggesting a progression toward type 2 diabetes. We found that hypothalamic LXR expression was altered in fructose-fed rats. Rats in the FG presented with a decrease in LXRb levels while showing an increase in LXRa expression in the hypothalamus but not in the hippocampus, cerebellum, or neocortex. Moreover, both LXRa and b expression correlated negatively with insulin and triglyceride levels. Interestingly, LXRb showed a negative correlation with the area under the curve during the glucose tolerance test in the CG and a positive correlation in the FG. Immunocytochemistry revealed that the paraventricular and ventromedial nuclei express mainly LXRa whereas the arcuate nucleus expresses LXRb. Both LXR immunosignals were found in the median preoptic area. This is the first study showing a relationship between glucose and lipid homeostasis and the expression of LXRs in the hypothalamus, suggesting that LXRs may trigger neurochemical and neurophysiological responses for the control of food intake and energy expenditure through these receptors.Fil: Kruse, Maria Sol. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Rey, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Vega, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Coirini, Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; Argentina. Universidad Catolica de Cuyo - Sede San Juan; ArgentinaBioScientifica2012-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/22184Kruse, Maria Sol; Rey, Mariana; Vega, Maria Cristina; Coirini, Hector; Alterations of LXRα and LXRβ expression in the hypothalamus of glucose-intolerant rats; BioScientifica; Journal of Endocrinology; 215; 1; 10-2012; 51-580022-07951479-6805CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://joe.endocrinology-journals.org/content/215/1/51.longinfo:eu-repo/semantics/altIdentifier/doi/ 10.1530/JOE-12-0088info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T11:23:02Zoai:ri.conicet.gov.ar:11336/22184instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 11:23:02.482CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Alterations of LXRα and LXRβ expression in the hypothalamus of glucose-intolerant rats |
| title |
Alterations of LXRα and LXRβ expression in the hypothalamus of glucose-intolerant rats |
| spellingShingle |
Alterations of LXRα and LXRβ expression in the hypothalamus of glucose-intolerant rats Kruse, Maria Sol Lxr Receptors Glucose Intolerant Hypothalamus |
| title_short |
Alterations of LXRα and LXRβ expression in the hypothalamus of glucose-intolerant rats |
| title_full |
Alterations of LXRα and LXRβ expression in the hypothalamus of glucose-intolerant rats |
| title_fullStr |
Alterations of LXRα and LXRβ expression in the hypothalamus of glucose-intolerant rats |
| title_full_unstemmed |
Alterations of LXRα and LXRβ expression in the hypothalamus of glucose-intolerant rats |
| title_sort |
Alterations of LXRα and LXRβ expression in the hypothalamus of glucose-intolerant rats |
| dc.creator.none.fl_str_mv |
Kruse, Maria Sol Rey, Mariana Vega, Maria Cristina Coirini, Hector |
| author |
Kruse, Maria Sol |
| author_facet |
Kruse, Maria Sol Rey, Mariana Vega, Maria Cristina Coirini, Hector |
| author_role |
author |
| author2 |
Rey, Mariana Vega, Maria Cristina Coirini, Hector |
| author2_role |
author author author |
| dc.subject.none.fl_str_mv |
Lxr Receptors Glucose Intolerant Hypothalamus |
| topic |
Lxr Receptors Glucose Intolerant Hypothalamus |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
| dc.description.none.fl_txt_mv |
Liver X receptor (LXR) a and b are nuclear receptors that are crucial for the regulation of carbohydrate and lipid metabolism. Activation of LXRs in the brain facilitates cholesterol clearance and improves cognitive deficits, thus they are considered as promising drug targets to treat diseases such as atherosclerosis and Alzheimer´s disease. Nevertheless, little is known about the function and localization of LXRs in the brain. Here, we studied the expression of LXR in the brains of rats that received free access to 10% (w/v) fructose group (FG) in their beverages or water control drinks (control group (CG)). After 6 weeks rats in the FG presented with hypertriglyceridemia, hyperinsulinemia, and became glucose intolerant, suggesting a progression toward type 2 diabetes. We found that hypothalamic LXR expression was altered in fructose-fed rats. Rats in the FG presented with a decrease in LXRb levels while showing an increase in LXRa expression in the hypothalamus but not in the hippocampus, cerebellum, or neocortex. Moreover, both LXRa and b expression correlated negatively with insulin and triglyceride levels. Interestingly, LXRb showed a negative correlation with the area under the curve during the glucose tolerance test in the CG and a positive correlation in the FG. Immunocytochemistry revealed that the paraventricular and ventromedial nuclei express mainly LXRa whereas the arcuate nucleus expresses LXRb. Both LXR immunosignals were found in the median preoptic area. This is the first study showing a relationship between glucose and lipid homeostasis and the expression of LXRs in the hypothalamus, suggesting that LXRs may trigger neurochemical and neurophysiological responses for the control of food intake and energy expenditure through these receptors. Fil: Kruse, Maria Sol. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina Fil: Rey, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina Fil: Vega, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina Fil: Coirini, Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Bioquímica Humana; Argentina. Universidad Catolica de Cuyo - Sede San Juan; Argentina |
| description |
Liver X receptor (LXR) a and b are nuclear receptors that are crucial for the regulation of carbohydrate and lipid metabolism. Activation of LXRs in the brain facilitates cholesterol clearance and improves cognitive deficits, thus they are considered as promising drug targets to treat diseases such as atherosclerosis and Alzheimer´s disease. Nevertheless, little is known about the function and localization of LXRs in the brain. Here, we studied the expression of LXR in the brains of rats that received free access to 10% (w/v) fructose group (FG) in their beverages or water control drinks (control group (CG)). After 6 weeks rats in the FG presented with hypertriglyceridemia, hyperinsulinemia, and became glucose intolerant, suggesting a progression toward type 2 diabetes. We found that hypothalamic LXR expression was altered in fructose-fed rats. Rats in the FG presented with a decrease in LXRb levels while showing an increase in LXRa expression in the hypothalamus but not in the hippocampus, cerebellum, or neocortex. Moreover, both LXRa and b expression correlated negatively with insulin and triglyceride levels. Interestingly, LXRb showed a negative correlation with the area under the curve during the glucose tolerance test in the CG and a positive correlation in the FG. Immunocytochemistry revealed that the paraventricular and ventromedial nuclei express mainly LXRa whereas the arcuate nucleus expresses LXRb. Both LXR immunosignals were found in the median preoptic area. This is the first study showing a relationship between glucose and lipid homeostasis and the expression of LXRs in the hypothalamus, suggesting that LXRs may trigger neurochemical and neurophysiological responses for the control of food intake and energy expenditure through these receptors. |
| publishDate |
2012 |
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2012-10 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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publishedVersion |
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http://hdl.handle.net/11336/22184 Kruse, Maria Sol; Rey, Mariana; Vega, Maria Cristina; Coirini, Hector; Alterations of LXRα and LXRβ expression in the hypothalamus of glucose-intolerant rats; BioScientifica; Journal of Endocrinology; 215; 1; 10-2012; 51-58 0022-0795 1479-6805 CONICET Digital CONICET |
| url |
http://hdl.handle.net/11336/22184 |
| identifier_str_mv |
Kruse, Maria Sol; Rey, Mariana; Vega, Maria Cristina; Coirini, Hector; Alterations of LXRα and LXRβ expression in the hypothalamus of glucose-intolerant rats; BioScientifica; Journal of Endocrinology; 215; 1; 10-2012; 51-58 0022-0795 1479-6805 CONICET Digital CONICET |
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eng |
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eng |
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