A subset of low density granulocytes is associated with vascular calcification in chronic kidney disease patients

Autores
Rodríguez Carrio, Javier; Carrillo López, Natalia; Ulloa, Catalina; Seijo, Mariana; Rodríguez García, Minerva; Rodríguez Suárez, Carmen; Díaz-Corte, Carmen; Cannata Andía, Jorge B.; Suárez, Ana; Dusso, Adriana
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Inflammation is central to chronic kidney disease (CKD) pathogenesis and vascular outcomes, but the exact players remain unidentified. Since low density granulocytes (LDGs) are emerging mediators in inflammatory conditions, we aimed to evaluate whether LDGs may be altered in CKD and related to clinical outcomes as biomarkers. To his end, LDGs subsets were measured in peripheral blood by flow cytometry and confocal microscopy in 33 CKD patients undergoing peritoneal dialysis and 15 healthy controls (HC). Analyses were replicated in an additional cohort. DEF3 (marker of early granulopoiesis) gene expression on PBMCs was quantified by qPCR. Total CD15+ LDGs and both CD14lowCD16+ and CD14−CD16− subsets were expanded in CKD. The relative frequency of the CD14−CD16− subpopulation was higher among the CD15+ pool in CKD. This alteration was stable over-time. The increased CD14−CD16−CD15+ paralleled Kauppila scores and DEF3 expression, whereas no association was found with CD14lowCD16+ CD15+. Both subsets differed in their CD11b, CD10, CD35, CD31, CD62L, IFNAR1 and CD68 expression, FSC/SSC features and nuclear morphology, pointing to different origins and maturation status. In conclusion, LDGs were expanded in CKD showing a skewed distribution towards a CD14−CD16−CD15+ enrichment, in association with vascular calcification. DEF3 expression in PBMC can be a marker of LDG expansion.
Fil: Rodríguez Carrio, Javier. Hospital Universitario Central de Asturias. Instituto de Investigación Sanitaria del Principado de Asturias (ISPA). Bone and Mineral Research Unit; España. Universidad de Oviedo; España
Fil: Carrillo López, Natalia. Hospital Universitario Central de Asturias; España
Fil: Ulloa, Catalina. Hospital Universitario Central de Asturias; España
Fil: Seijo, Mariana. Hospital Universitario Central de Asturias; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina
Fil: Rodríguez García, Minerva. Hospital Universitario Central de Asturias; España
Fil: Rodríguez Suárez, Carmen. Hospital Universitario Central de Asturias; España
Fil: Díaz-Corte, Carmen. Hospital Universitario Central de Asturias; España
Fil: Cannata Andía, Jorge B.. Universidad de Oviedo; España. Hospital Universitario Central de Asturias; España
Fil: Suárez, Ana. Universidad de Oviedo; España
Fil: Dusso, Adriana. Hospital Universitario Central de Asturias; España
Materia
diagnostic markers
traslational research
kidney disease
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/116165
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/116165
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A subset of low density granulocytes is associated with vascular calcification in chronic kidney disease patientsRodríguez Carrio, JavierCarrillo López, NataliaUlloa, CatalinaSeijo, MarianaRodríguez García, MinervaRodríguez Suárez, CarmenDíaz-Corte, CarmenCannata Andía, Jorge B.Suárez, AnaDusso, Adrianadiagnostic markerstraslational researchkidney diseasehttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Inflammation is central to chronic kidney disease (CKD) pathogenesis and vascular outcomes, but the exact players remain unidentified. Since low density granulocytes (LDGs) are emerging mediators in inflammatory conditions, we aimed to evaluate whether LDGs may be altered in CKD and related to clinical outcomes as biomarkers. To his end, LDGs subsets were measured in peripheral blood by flow cytometry and confocal microscopy in 33 CKD patients undergoing peritoneal dialysis and 15 healthy controls (HC). Analyses were replicated in an additional cohort. DEF3 (marker of early granulopoiesis) gene expression on PBMCs was quantified by qPCR. Total CD15+ LDGs and both CD14lowCD16+ and CD14−CD16− subsets were expanded in CKD. The relative frequency of the CD14−CD16− subpopulation was higher among the CD15+ pool in CKD. This alteration was stable over-time. The increased CD14−CD16−CD15+ paralleled Kauppila scores and DEF3 expression, whereas no association was found with CD14lowCD16+ CD15+. Both subsets differed in their CD11b, CD10, CD35, CD31, CD62L, IFNAR1 and CD68 expression, FSC/SSC features and nuclear morphology, pointing to different origins and maturation status. In conclusion, LDGs were expanded in CKD showing a skewed distribution towards a CD14−CD16−CD15+ enrichment, in association with vascular calcification. DEF3 expression in PBMC can be a marker of LDG expansion.Fil: Rodríguez Carrio, Javier. Hospital Universitario Central de Asturias. Instituto de Investigación Sanitaria del Principado de Asturias (ISPA). Bone and Mineral Research Unit; España. Universidad de Oviedo; EspañaFil: Carrillo López, Natalia. Hospital Universitario Central de Asturias; EspañaFil: Ulloa, Catalina. Hospital Universitario Central de Asturias; EspañaFil: Seijo, Mariana. Hospital Universitario Central de Asturias; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; ArgentinaFil: Rodríguez García, Minerva. Hospital Universitario Central de Asturias; EspañaFil: Rodríguez Suárez, Carmen. Hospital Universitario Central de Asturias; EspañaFil: Díaz-Corte, Carmen. Hospital Universitario Central de Asturias; EspañaFil: Cannata Andía, Jorge B.. Universidad de Oviedo; España. Hospital Universitario Central de Asturias; EspañaFil: Suárez, Ana. Universidad de Oviedo; EspañaFil: Dusso, Adriana. Hospital Universitario Central de Asturias; EspañaNature Publishing Group2019-09-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/116165Rodríguez Carrio, Javier; Carrillo López, Natalia; Ulloa, Catalina; Seijo, Mariana; Rodríguez García, Minerva; et al.; A subset of low density granulocytes is associated with vascular calcification in chronic kidney disease patients; Nature Publishing Group; Scientific Reports; 9; 1; 13-9-2019; 1-112045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.nature.com/articles/s41598-019-49429-xinfo:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-019-49429-xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-02-26T10:26:03Zoai:ri.conicet.gov.ar:11336/116165instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-02-26 10:26:03.683CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A subset of low density granulocytes is associated with vascular calcification in chronic kidney disease patients
title A subset of low density granulocytes is associated with vascular calcification in chronic kidney disease patients
spellingShingle A subset of low density granulocytes is associated with vascular calcification in chronic kidney disease patients
Rodríguez Carrio, Javier
diagnostic markers
traslational research
kidney disease
title_short A subset of low density granulocytes is associated with vascular calcification in chronic kidney disease patients
title_full A subset of low density granulocytes is associated with vascular calcification in chronic kidney disease patients
title_fullStr A subset of low density granulocytes is associated with vascular calcification in chronic kidney disease patients
title_full_unstemmed A subset of low density granulocytes is associated with vascular calcification in chronic kidney disease patients
title_sort A subset of low density granulocytes is associated with vascular calcification in chronic kidney disease patients
dc.creator.none.fl_str_mv Rodríguez Carrio, Javier
Carrillo López, Natalia
Ulloa, Catalina
Seijo, Mariana
Rodríguez García, Minerva
Rodríguez Suárez, Carmen
Díaz-Corte, Carmen
Cannata Andía, Jorge B.
Suárez, Ana
Dusso, Adriana
author Rodríguez Carrio, Javier
author_facet Rodríguez Carrio, Javier
Carrillo López, Natalia
Ulloa, Catalina
Seijo, Mariana
Rodríguez García, Minerva
Rodríguez Suárez, Carmen
Díaz-Corte, Carmen
Cannata Andía, Jorge B.
Suárez, Ana
Dusso, Adriana
author_role author
author2 Carrillo López, Natalia
Ulloa, Catalina
Seijo, Mariana
Rodríguez García, Minerva
Rodríguez Suárez, Carmen
Díaz-Corte, Carmen
Cannata Andía, Jorge B.
Suárez, Ana
Dusso, Adriana
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv diagnostic markers
traslational research
kidney disease
topic diagnostic markers
traslational research
kidney disease
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Inflammation is central to chronic kidney disease (CKD) pathogenesis and vascular outcomes, but the exact players remain unidentified. Since low density granulocytes (LDGs) are emerging mediators in inflammatory conditions, we aimed to evaluate whether LDGs may be altered in CKD and related to clinical outcomes as biomarkers. To his end, LDGs subsets were measured in peripheral blood by flow cytometry and confocal microscopy in 33 CKD patients undergoing peritoneal dialysis and 15 healthy controls (HC). Analyses were replicated in an additional cohort. DEF3 (marker of early granulopoiesis) gene expression on PBMCs was quantified by qPCR. Total CD15+ LDGs and both CD14lowCD16+ and CD14−CD16− subsets were expanded in CKD. The relative frequency of the CD14−CD16− subpopulation was higher among the CD15+ pool in CKD. This alteration was stable over-time. The increased CD14−CD16−CD15+ paralleled Kauppila scores and DEF3 expression, whereas no association was found with CD14lowCD16+ CD15+. Both subsets differed in their CD11b, CD10, CD35, CD31, CD62L, IFNAR1 and CD68 expression, FSC/SSC features and nuclear morphology, pointing to different origins and maturation status. In conclusion, LDGs were expanded in CKD showing a skewed distribution towards a CD14−CD16−CD15+ enrichment, in association with vascular calcification. DEF3 expression in PBMC can be a marker of LDG expansion.
Fil: Rodríguez Carrio, Javier. Hospital Universitario Central de Asturias. Instituto de Investigación Sanitaria del Principado de Asturias (ISPA). Bone and Mineral Research Unit; España. Universidad de Oviedo; España
Fil: Carrillo López, Natalia. Hospital Universitario Central de Asturias; España
Fil: Ulloa, Catalina. Hospital Universitario Central de Asturias; España
Fil: Seijo, Mariana. Hospital Universitario Central de Asturias; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina
Fil: Rodríguez García, Minerva. Hospital Universitario Central de Asturias; España
Fil: Rodríguez Suárez, Carmen. Hospital Universitario Central de Asturias; España
Fil: Díaz-Corte, Carmen. Hospital Universitario Central de Asturias; España
Fil: Cannata Andía, Jorge B.. Universidad de Oviedo; España. Hospital Universitario Central de Asturias; España
Fil: Suárez, Ana. Universidad de Oviedo; España
Fil: Dusso, Adriana. Hospital Universitario Central de Asturias; España
description Inflammation is central to chronic kidney disease (CKD) pathogenesis and vascular outcomes, but the exact players remain unidentified. Since low density granulocytes (LDGs) are emerging mediators in inflammatory conditions, we aimed to evaluate whether LDGs may be altered in CKD and related to clinical outcomes as biomarkers. To his end, LDGs subsets were measured in peripheral blood by flow cytometry and confocal microscopy in 33 CKD patients undergoing peritoneal dialysis and 15 healthy controls (HC). Analyses were replicated in an additional cohort. DEF3 (marker of early granulopoiesis) gene expression on PBMCs was quantified by qPCR. Total CD15+ LDGs and both CD14lowCD16+ and CD14−CD16− subsets were expanded in CKD. The relative frequency of the CD14−CD16− subpopulation was higher among the CD15+ pool in CKD. This alteration was stable over-time. The increased CD14−CD16−CD15+ paralleled Kauppila scores and DEF3 expression, whereas no association was found with CD14lowCD16+ CD15+. Both subsets differed in their CD11b, CD10, CD35, CD31, CD62L, IFNAR1 and CD68 expression, FSC/SSC features and nuclear morphology, pointing to different origins and maturation status. In conclusion, LDGs were expanded in CKD showing a skewed distribution towards a CD14−CD16−CD15+ enrichment, in association with vascular calcification. DEF3 expression in PBMC can be a marker of LDG expansion.
publishDate 2019
dc.date.none.fl_str_mv 2019-09-13
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/116165
Rodríguez Carrio, Javier; Carrillo López, Natalia; Ulloa, Catalina; Seijo, Mariana; Rodríguez García, Minerva; et al.; A subset of low density granulocytes is associated with vascular calcification in chronic kidney disease patients; Nature Publishing Group; Scientific Reports; 9; 1; 13-9-2019; 1-11
2045-2322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/116165
identifier_str_mv Rodríguez Carrio, Javier; Carrillo López, Natalia; Ulloa, Catalina; Seijo, Mariana; Rodríguez García, Minerva; et al.; A subset of low density granulocytes is associated with vascular calcification in chronic kidney disease patients; Nature Publishing Group; Scientific Reports; 9; 1; 13-9-2019; 1-11
2045-2322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.nature.com/articles/s41598-019-49429-x
info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-019-49429-x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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