Calcitonin modifies ligand binding to muscarinic receptor in CNS membranes

Autores
Rodriguez, Georgina Emma; Schneider, Patricia Graciela
Año de publicación
2000
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Calcitonin (CT) is a peptide produced by the thyroid gland, whose best described role is to prevent bone reabsorption, though it also participates in other biological functions through both central and peripheral mechanisms. CT is able to inhibit brain Na+, K+-ATPase activity (Rodriguez de Lores Arnaiz, Lopez Ordieres, Peptides 1997;18:613-5) and a relationship between such enzyme activity and cholinergic function has been suggested. Accordingly, we tested CT effect on [3H]-quinuclidinyl benzilate ([3H]-QNB) binding to rat CNS membranes to determine whether the peptide is able to modify the cholinergic muscarinic receptor as well. It was found that 1x10-7-1x10-5 M CT decreased 20-70% ligand binding to hippocampal, cerebellar, cortical and striatal membranes. Scatchard analysis of saturation curves showed that 5x10-6 M CT significantly modified binding kinetic constants, thus it increased roughly 220% Kd values and decreased 20-36% Bmax values in cerebral cortical and cerebellar membranes. Since the peptide decreases affinity ligand binding and reduces the number of binding sites, CT may well be acting as a cholinergic modulator through a decrease in muscarinic receptor functionality. © 2000 Elsevier Science B.V.
Fil: Rodriguez, Georgina Emma. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia ; Argentina
Fil: Schneider, Patricia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia ; Argentina
Materia
Calcitonin Effect
Cholinergic Muscarinic Receptor
Cholinergic System
Cns Membranes
Na+, K+-Atpase
Qnb Binding
Sodium Pump
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/39154
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/39154
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Calcitonin modifies ligand binding to muscarinic receptor in CNS membranesRodriguez, Georgina EmmaSchneider, Patricia GracielaCalcitonin EffectCholinergic Muscarinic ReceptorCholinergic SystemCns MembranesNa+, K+-AtpaseQnb BindingSodium Pumphttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Calcitonin (CT) is a peptide produced by the thyroid gland, whose best described role is to prevent bone reabsorption, though it also participates in other biological functions through both central and peripheral mechanisms. CT is able to inhibit brain Na+, K+-ATPase activity (Rodriguez de Lores Arnaiz, Lopez Ordieres, Peptides 1997;18:613-5) and a relationship between such enzyme activity and cholinergic function has been suggested. Accordingly, we tested CT effect on [3H]-quinuclidinyl benzilate ([3H]-QNB) binding to rat CNS membranes to determine whether the peptide is able to modify the cholinergic muscarinic receptor as well. It was found that 1x10-7-1x10-5 M CT decreased 20-70% ligand binding to hippocampal, cerebellar, cortical and striatal membranes. Scatchard analysis of saturation curves showed that 5x10-6 M CT significantly modified binding kinetic constants, thus it increased roughly 220% Kd values and decreased 20-36% Bmax values in cerebral cortical and cerebellar membranes. Since the peptide decreases affinity ligand binding and reduces the number of binding sites, CT may well be acting as a cholinergic modulator through a decrease in muscarinic receptor functionality. © 2000 Elsevier Science B.V.Fil: Rodriguez, Georgina Emma. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia ; ArgentinaFil: Schneider, Patricia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia ; ArgentinaElsevier Science2000-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/39154Rodriguez, Georgina Emma; Schneider, Patricia Graciela; Calcitonin modifies ligand binding to muscarinic receptor in CNS membranes; Elsevier Science; Regulatory Peptides; 88; 1-3; 3-2000; 21-260167-0115CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/S0167-0115(99)00109-3info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0167011599001093info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:33:31Zoai:ri.conicet.gov.ar:11336/39154instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:33:31.487CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Calcitonin modifies ligand binding to muscarinic receptor in CNS membranes
title Calcitonin modifies ligand binding to muscarinic receptor in CNS membranes
spellingShingle Calcitonin modifies ligand binding to muscarinic receptor in CNS membranes
Rodriguez, Georgina Emma
Calcitonin Effect
Cholinergic Muscarinic Receptor
Cholinergic System
Cns Membranes
Na+, K+-Atpase
Qnb Binding
Sodium Pump
title_short Calcitonin modifies ligand binding to muscarinic receptor in CNS membranes
title_full Calcitonin modifies ligand binding to muscarinic receptor in CNS membranes
title_fullStr Calcitonin modifies ligand binding to muscarinic receptor in CNS membranes
title_full_unstemmed Calcitonin modifies ligand binding to muscarinic receptor in CNS membranes
title_sort Calcitonin modifies ligand binding to muscarinic receptor in CNS membranes
dc.creator.none.fl_str_mv Rodriguez, Georgina Emma
Schneider, Patricia Graciela
author Rodriguez, Georgina Emma
author_facet Rodriguez, Georgina Emma
Schneider, Patricia Graciela
author_role author
author2 Schneider, Patricia Graciela
author2_role author
dc.subject.none.fl_str_mv Calcitonin Effect
Cholinergic Muscarinic Receptor
Cholinergic System
Cns Membranes
Na+, K+-Atpase
Qnb Binding
Sodium Pump
topic Calcitonin Effect
Cholinergic Muscarinic Receptor
Cholinergic System
Cns Membranes
Na+, K+-Atpase
Qnb Binding
Sodium Pump
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Calcitonin (CT) is a peptide produced by the thyroid gland, whose best described role is to prevent bone reabsorption, though it also participates in other biological functions through both central and peripheral mechanisms. CT is able to inhibit brain Na+, K+-ATPase activity (Rodriguez de Lores Arnaiz, Lopez Ordieres, Peptides 1997;18:613-5) and a relationship between such enzyme activity and cholinergic function has been suggested. Accordingly, we tested CT effect on [3H]-quinuclidinyl benzilate ([3H]-QNB) binding to rat CNS membranes to determine whether the peptide is able to modify the cholinergic muscarinic receptor as well. It was found that 1x10-7-1x10-5 M CT decreased 20-70% ligand binding to hippocampal, cerebellar, cortical and striatal membranes. Scatchard analysis of saturation curves showed that 5x10-6 M CT significantly modified binding kinetic constants, thus it increased roughly 220% Kd values and decreased 20-36% Bmax values in cerebral cortical and cerebellar membranes. Since the peptide decreases affinity ligand binding and reduces the number of binding sites, CT may well be acting as a cholinergic modulator through a decrease in muscarinic receptor functionality. © 2000 Elsevier Science B.V.
Fil: Rodriguez, Georgina Emma. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia ; Argentina
Fil: Schneider, Patricia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia ; Argentina
description Calcitonin (CT) is a peptide produced by the thyroid gland, whose best described role is to prevent bone reabsorption, though it also participates in other biological functions through both central and peripheral mechanisms. CT is able to inhibit brain Na+, K+-ATPase activity (Rodriguez de Lores Arnaiz, Lopez Ordieres, Peptides 1997;18:613-5) and a relationship between such enzyme activity and cholinergic function has been suggested. Accordingly, we tested CT effect on [3H]-quinuclidinyl benzilate ([3H]-QNB) binding to rat CNS membranes to determine whether the peptide is able to modify the cholinergic muscarinic receptor as well. It was found that 1x10-7-1x10-5 M CT decreased 20-70% ligand binding to hippocampal, cerebellar, cortical and striatal membranes. Scatchard analysis of saturation curves showed that 5x10-6 M CT significantly modified binding kinetic constants, thus it increased roughly 220% Kd values and decreased 20-36% Bmax values in cerebral cortical and cerebellar membranes. Since the peptide decreases affinity ligand binding and reduces the number of binding sites, CT may well be acting as a cholinergic modulator through a decrease in muscarinic receptor functionality. © 2000 Elsevier Science B.V.
publishDate 2000
dc.date.none.fl_str_mv 2000-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/39154
Rodriguez, Georgina Emma; Schneider, Patricia Graciela; Calcitonin modifies ligand binding to muscarinic receptor in CNS membranes; Elsevier Science; Regulatory Peptides; 88; 1-3; 3-2000; 21-26
0167-0115
CONICET Digital
CONICET
url http://hdl.handle.net/11336/39154
identifier_str_mv Rodriguez, Georgina Emma; Schneider, Patricia Graciela; Calcitonin modifies ligand binding to muscarinic receptor in CNS membranes; Elsevier Science; Regulatory Peptides; 88; 1-3; 3-2000; 21-26
0167-0115
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/S0167-0115(99)00109-3
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0167011599001093
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614350780760064
score 13.070432

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