Ca2+ signaling in injured in situ endothelium of rat aorta

Autores
Berra Romani, Roberto; Raqeeb, Abdul; Avelino Cruz, José Everardo; Moccia, Francesco; Oldani, Amanda; Speroni Aguirre, Francisco José; Taglietti, Vanni; Tanzi, Franco
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The inner wall of excised rat aorta was scraped by a microelectrode and Ca2+ signals were investigated by fluorescence microscopy in endothelial cells (ECs) directly coupled with injured cells. The injury caused an immediate increase in the intracellular Ca2+ concentration ([Ca2+]i), followed by a long-lasting decay phase due to Ca2+ influx from extracellular space. The immediate response was mainly due to activation of purinergic receptors, as shown by the effect of P2X and P2Y receptors agonists and antagonists, such as suramin, α,β-MeATP, MRS-2179 and 2-MeSAMP. Inhibition of store-operated Ca2+ influx did not affect either the peak response or the decay phase. Furthermore, the latter was: (i) insensitive to phospholipase C inhibition, (ii) sensitive to the gap junction blockers, palmitoleic acid, heptanol, octanol and oleamide, and (iii) sensitive to La3+ and Ni2+, but not to Gd3+. Finally, ethidium bromide or Lucifer Yellow did not enter ECs facing the scraped area. These results suggest that endothelium scraping: (i) causes a short-lasting stimulation of healthy ECs by extracellular nucleotides released from damaged cells and (ii) uncouples the hemichannels of the ECs facing the injury site; these hemichannels do not fully close and allow a long-lasting Ca2+ entry.
Fil: Berra Romani, Roberto. Universita degli Studi di Pavia; Italia
Fil: Raqeeb, Abdul. Universita degli Studi di Pavia; Italia
Fil: Avelino Cruz, José Everardo. Universita degli Studi di Pavia; Italia
Fil: Moccia, Francesco. Universita degli Studi di Pavia; Italia
Fil: Oldani, Amanda. Universita degli Studi di Pavia; Italia
Fil: Speroni Aguirre, Francisco José. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina. Universita degli Studi di Pavia; Italia
Fil: Taglietti, Vanni. Universita degli Studi di Pavia; Italia
Fil: Tanzi, Franco. Universita degli Studi di Pavia; Italia
Materia
ATP
CA2+
GAP JUNCTIONS
IN SITU ENDOTHELIUM
INJURY
RAT AORTA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/159337
Acceder
id CONICETDig_2c24d2f2c18ccf00f1ab6f13058b7633
oai_identifier_str oai:ri.conicet.gov.ar:11336/159337
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Ca2+ signaling in injured in situ endothelium of rat aortaBerra Romani, RobertoRaqeeb, AbdulAvelino Cruz, José EverardoMoccia, FrancescoOldani, AmandaSperoni Aguirre, Francisco JoséTaglietti, VanniTanzi, FrancoATPCA2+GAP JUNCTIONSIN SITU ENDOTHELIUMINJURYRAT AORTAhttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2The inner wall of excised rat aorta was scraped by a microelectrode and Ca2+ signals were investigated by fluorescence microscopy in endothelial cells (ECs) directly coupled with injured cells. The injury caused an immediate increase in the intracellular Ca2+ concentration ([Ca2+]i), followed by a long-lasting decay phase due to Ca2+ influx from extracellular space. The immediate response was mainly due to activation of purinergic receptors, as shown by the effect of P2X and P2Y receptors agonists and antagonists, such as suramin, α,β-MeATP, MRS-2179 and 2-MeSAMP. Inhibition of store-operated Ca2+ influx did not affect either the peak response or the decay phase. Furthermore, the latter was: (i) insensitive to phospholipase C inhibition, (ii) sensitive to the gap junction blockers, palmitoleic acid, heptanol, octanol and oleamide, and (iii) sensitive to La3+ and Ni2+, but not to Gd3+. Finally, ethidium bromide or Lucifer Yellow did not enter ECs facing the scraped area. These results suggest that endothelium scraping: (i) causes a short-lasting stimulation of healthy ECs by extracellular nucleotides released from damaged cells and (ii) uncouples the hemichannels of the ECs facing the injury site; these hemichannels do not fully close and allow a long-lasting Ca2+ entry.Fil: Berra Romani, Roberto. Universita degli Studi di Pavia; ItaliaFil: Raqeeb, Abdul. Universita degli Studi di Pavia; ItaliaFil: Avelino Cruz, José Everardo. Universita degli Studi di Pavia; ItaliaFil: Moccia, Francesco. Universita degli Studi di Pavia; ItaliaFil: Oldani, Amanda. Universita degli Studi di Pavia; ItaliaFil: Speroni Aguirre, Francisco José. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina. Universita degli Studi di Pavia; ItaliaFil: Taglietti, Vanni. Universita degli Studi di Pavia; ItaliaFil: Tanzi, Franco. Universita degli Studi di Pavia; ItaliaElsevier2008-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/159337Berra Romani, Roberto; Raqeeb, Abdul; Avelino Cruz, José Everardo; Moccia, Francesco; Oldani, Amanda; et al.; Ca2+ signaling in injured in situ endothelium of rat aorta; Elsevier; Cell Calcium; 44; 3; 9-2008; 298-3090143-41601532-1991CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0143416007002138info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ceca.2007.12.007info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-05T10:34:25Zoai:ri.conicet.gov.ar:11336/159337instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-05 10:34:25.607CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Ca2+ signaling in injured in situ endothelium of rat aorta
title Ca2+ signaling in injured in situ endothelium of rat aorta
spellingShingle Ca2+ signaling in injured in situ endothelium of rat aorta
Berra Romani, Roberto
ATP
CA2+
GAP JUNCTIONS
IN SITU ENDOTHELIUM
INJURY
RAT AORTA
title_short Ca2+ signaling in injured in situ endothelium of rat aorta
title_full Ca2+ signaling in injured in situ endothelium of rat aorta
title_fullStr Ca2+ signaling in injured in situ endothelium of rat aorta
title_full_unstemmed Ca2+ signaling in injured in situ endothelium of rat aorta
title_sort Ca2+ signaling in injured in situ endothelium of rat aorta
dc.creator.none.fl_str_mv Berra Romani, Roberto
Raqeeb, Abdul
Avelino Cruz, José Everardo
Moccia, Francesco
Oldani, Amanda
Speroni Aguirre, Francisco José
Taglietti, Vanni
Tanzi, Franco
author Berra Romani, Roberto
author_facet Berra Romani, Roberto
Raqeeb, Abdul
Avelino Cruz, José Everardo
Moccia, Francesco
Oldani, Amanda
Speroni Aguirre, Francisco José
Taglietti, Vanni
Tanzi, Franco
author_role author
author2 Raqeeb, Abdul
Avelino Cruz, José Everardo
Moccia, Francesco
Oldani, Amanda
Speroni Aguirre, Francisco José
Taglietti, Vanni
Tanzi, Franco
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ATP
CA2+
GAP JUNCTIONS
IN SITU ENDOTHELIUM
INJURY
RAT AORTA
topic ATP
CA2+
GAP JUNCTIONS
IN SITU ENDOTHELIUM
INJURY
RAT AORTA
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The inner wall of excised rat aorta was scraped by a microelectrode and Ca2+ signals were investigated by fluorescence microscopy in endothelial cells (ECs) directly coupled with injured cells. The injury caused an immediate increase in the intracellular Ca2+ concentration ([Ca2+]i), followed by a long-lasting decay phase due to Ca2+ influx from extracellular space. The immediate response was mainly due to activation of purinergic receptors, as shown by the effect of P2X and P2Y receptors agonists and antagonists, such as suramin, α,β-MeATP, MRS-2179 and 2-MeSAMP. Inhibition of store-operated Ca2+ influx did not affect either the peak response or the decay phase. Furthermore, the latter was: (i) insensitive to phospholipase C inhibition, (ii) sensitive to the gap junction blockers, palmitoleic acid, heptanol, octanol and oleamide, and (iii) sensitive to La3+ and Ni2+, but not to Gd3+. Finally, ethidium bromide or Lucifer Yellow did not enter ECs facing the scraped area. These results suggest that endothelium scraping: (i) causes a short-lasting stimulation of healthy ECs by extracellular nucleotides released from damaged cells and (ii) uncouples the hemichannels of the ECs facing the injury site; these hemichannels do not fully close and allow a long-lasting Ca2+ entry.
Fil: Berra Romani, Roberto. Universita degli Studi di Pavia; Italia
Fil: Raqeeb, Abdul. Universita degli Studi di Pavia; Italia
Fil: Avelino Cruz, José Everardo. Universita degli Studi di Pavia; Italia
Fil: Moccia, Francesco. Universita degli Studi di Pavia; Italia
Fil: Oldani, Amanda. Universita degli Studi di Pavia; Italia
Fil: Speroni Aguirre, Francisco José. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina. Universita degli Studi di Pavia; Italia
Fil: Taglietti, Vanni. Universita degli Studi di Pavia; Italia
Fil: Tanzi, Franco. Universita degli Studi di Pavia; Italia
description The inner wall of excised rat aorta was scraped by a microelectrode and Ca2+ signals were investigated by fluorescence microscopy in endothelial cells (ECs) directly coupled with injured cells. The injury caused an immediate increase in the intracellular Ca2+ concentration ([Ca2+]i), followed by a long-lasting decay phase due to Ca2+ influx from extracellular space. The immediate response was mainly due to activation of purinergic receptors, as shown by the effect of P2X and P2Y receptors agonists and antagonists, such as suramin, α,β-MeATP, MRS-2179 and 2-MeSAMP. Inhibition of store-operated Ca2+ influx did not affect either the peak response or the decay phase. Furthermore, the latter was: (i) insensitive to phospholipase C inhibition, (ii) sensitive to the gap junction blockers, palmitoleic acid, heptanol, octanol and oleamide, and (iii) sensitive to La3+ and Ni2+, but not to Gd3+. Finally, ethidium bromide or Lucifer Yellow did not enter ECs facing the scraped area. These results suggest that endothelium scraping: (i) causes a short-lasting stimulation of healthy ECs by extracellular nucleotides released from damaged cells and (ii) uncouples the hemichannels of the ECs facing the injury site; these hemichannels do not fully close and allow a long-lasting Ca2+ entry.
publishDate 2008
dc.date.none.fl_str_mv 2008-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/159337
Berra Romani, Roberto; Raqeeb, Abdul; Avelino Cruz, José Everardo; Moccia, Francesco; Oldani, Amanda; et al.; Ca2+ signaling in injured in situ endothelium of rat aorta; Elsevier; Cell Calcium; 44; 3; 9-2008; 298-309
0143-4160
1532-1991
CONICET Digital
CONICET
url http://hdl.handle.net/11336/159337
identifier_str_mv Berra Romani, Roberto; Raqeeb, Abdul; Avelino Cruz, José Everardo; Moccia, Francesco; Oldani, Amanda; et al.; Ca2+ signaling in injured in situ endothelium of rat aorta; Elsevier; Cell Calcium; 44; 3; 9-2008; 298-309
0143-4160
1532-1991
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0143416007002138
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ceca.2007.12.007
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.087074

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