Differential regulation of endothelium behavior by progesterone and medroxyprogesterone acetate

Autores
Cutini, Pablo Hernan; Campelo, Adrián Esteban; Massheimer, Virginia Laura
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Medroxyprogesterone acetate (MPA) is a synthetic progestin commonly used in hormone replacement therapy (HRT). The aim of this work was to study and compare the effect of progesterone (Pg) and MPA, on the regulation of cellular events associated with vascular homeostasis and disease. Platelet adhesion to endothelial cells (ECs), nitric oxide (NO) production, and cell migration were studied using murine endothelial cells in vitro exposed to the progestins. After seven minutes treatment, MPA significantly inhibited NO synthesis with respect to control value; meanwhile Pg markedly increased vasoactive production. In senile ECs, the stimulatory action of Pg decreases; meanwhile MPA maintained its ability to inhibit NO synthesis.The presence of RU486 antagonized each steroid action. When ECs were preincubated with PD98059 (MAPK inhibitor) or chelerythrine (PKC inhibitor) before Pg or MPA treatment, the former totally suppressed the steroid action, but the PKC antagonist did not affect NO production. In the presence of a PI3K inhibitor (LY294002) a partial reduction in Pg effect, and a reversal of MPA action was detected. Using indomethacin the contribution of cyclooxygenase (COX) pathway was also detected. On platelet adhesion assays, Pg inhibited and MPA stimulated platelet adhesion to ECs. Under inflammatory conditions, Pg prevented platelet adhesion induced by lipopolysaccharide (LPS); meanwhile MPA potentiated the stimulatory action of LPS. Finally, although both steroids enhanced ECs migration, MPA exhibited a greater effect. In conclusion the data presented in this research provide evidence of a differential regulation of vascular function by Pg and MPA
Fil: Cutini, Pablo Hernan. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Campelo, Adrián Esteban. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahia Blanca; Argentina
Fil: Massheimer, Virginia Laura. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahia Blanca; Argentina
Materia
Endothelium
Progesterone
Medroxiprogesterone
Vascular
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/7452
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/7452
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Differential regulation of endothelium behavior by progesterone and medroxyprogesterone acetateCutini, Pablo HernanCampelo, Adrián EstebanMassheimer, Virginia LauraEndotheliumProgesteroneMedroxiprogesteroneVascularhttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Medroxyprogesterone acetate (MPA) is a synthetic progestin commonly used in hormone replacement therapy (HRT). The aim of this work was to study and compare the effect of progesterone (Pg) and MPA, on the regulation of cellular events associated with vascular homeostasis and disease. Platelet adhesion to endothelial cells (ECs), nitric oxide (NO) production, and cell migration were studied using murine endothelial cells in vitro exposed to the progestins. After seven minutes treatment, MPA significantly inhibited NO synthesis with respect to control value; meanwhile Pg markedly increased vasoactive production. In senile ECs, the stimulatory action of Pg decreases; meanwhile MPA maintained its ability to inhibit NO synthesis.The presence of RU486 antagonized each steroid action. When ECs were preincubated with PD98059 (MAPK inhibitor) or chelerythrine (PKC inhibitor) before Pg or MPA treatment, the former totally suppressed the steroid action, but the PKC antagonist did not affect NO production. In the presence of a PI3K inhibitor (LY294002) a partial reduction in Pg effect, and a reversal of MPA action was detected. Using indomethacin the contribution of cyclooxygenase (COX) pathway was also detected. On platelet adhesion assays, Pg inhibited and MPA stimulated platelet adhesion to ECs. Under inflammatory conditions, Pg prevented platelet adhesion induced by lipopolysaccharide (LPS); meanwhile MPA potentiated the stimulatory action of LPS. Finally, although both steroids enhanced ECs migration, MPA exhibited a greater effect. In conclusion the data presented in this research provide evidence of a differential regulation of vascular function by Pg and MPAFil: Cutini, Pablo Hernan. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Campelo, Adrián Esteban. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahia Blanca; ArgentinaFil: Massheimer, Virginia Laura. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahia Blanca; ArgentinaBioscientifica2013-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/rarapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/7452Cutini, Pablo Hernan; Campelo, Adrián Esteban; Massheimer, Virginia Laura; Differential regulation of endothelium behavior by progesterone and medroxyprogesterone acetate; Bioscientifica; Journal Of Endocrinology; 220; 12-2013; 179-1930022-0795enginfo:eu-repo/semantics/altIdentifier/url/http://joe.endocrinology-journals.org/content/220/3/179.longinfo:eu-repo/semantics/altIdentifier/doi/10.1530/JOE-13-0263info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:46:23Zoai:ri.conicet.gov.ar:11336/7452instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:46:24.049CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Differential regulation of endothelium behavior by progesterone and medroxyprogesterone acetate
title Differential regulation of endothelium behavior by progesterone and medroxyprogesterone acetate
spellingShingle Differential regulation of endothelium behavior by progesterone and medroxyprogesterone acetate
Cutini, Pablo Hernan
Endothelium
Progesterone
Medroxiprogesterone
Vascular
title_short Differential regulation of endothelium behavior by progesterone and medroxyprogesterone acetate
title_full Differential regulation of endothelium behavior by progesterone and medroxyprogesterone acetate
title_fullStr Differential regulation of endothelium behavior by progesterone and medroxyprogesterone acetate
title_full_unstemmed Differential regulation of endothelium behavior by progesterone and medroxyprogesterone acetate
title_sort Differential regulation of endothelium behavior by progesterone and medroxyprogesterone acetate
dc.creator.none.fl_str_mv Cutini, Pablo Hernan
Campelo, Adrián Esteban
Massheimer, Virginia Laura
author Cutini, Pablo Hernan
author_facet Cutini, Pablo Hernan
Campelo, Adrián Esteban
Massheimer, Virginia Laura
author_role author
author2 Campelo, Adrián Esteban
Massheimer, Virginia Laura
author2_role author
author
dc.subject.none.fl_str_mv Endothelium
Progesterone
Medroxiprogesterone
Vascular
topic Endothelium
Progesterone
Medroxiprogesterone
Vascular
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Medroxyprogesterone acetate (MPA) is a synthetic progestin commonly used in hormone replacement therapy (HRT). The aim of this work was to study and compare the effect of progesterone (Pg) and MPA, on the regulation of cellular events associated with vascular homeostasis and disease. Platelet adhesion to endothelial cells (ECs), nitric oxide (NO) production, and cell migration were studied using murine endothelial cells in vitro exposed to the progestins. After seven minutes treatment, MPA significantly inhibited NO synthesis with respect to control value; meanwhile Pg markedly increased vasoactive production. In senile ECs, the stimulatory action of Pg decreases; meanwhile MPA maintained its ability to inhibit NO synthesis.The presence of RU486 antagonized each steroid action. When ECs were preincubated with PD98059 (MAPK inhibitor) or chelerythrine (PKC inhibitor) before Pg or MPA treatment, the former totally suppressed the steroid action, but the PKC antagonist did not affect NO production. In the presence of a PI3K inhibitor (LY294002) a partial reduction in Pg effect, and a reversal of MPA action was detected. Using indomethacin the contribution of cyclooxygenase (COX) pathway was also detected. On platelet adhesion assays, Pg inhibited and MPA stimulated platelet adhesion to ECs. Under inflammatory conditions, Pg prevented platelet adhesion induced by lipopolysaccharide (LPS); meanwhile MPA potentiated the stimulatory action of LPS. Finally, although both steroids enhanced ECs migration, MPA exhibited a greater effect. In conclusion the data presented in this research provide evidence of a differential regulation of vascular function by Pg and MPA
Fil: Cutini, Pablo Hernan. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Campelo, Adrián Esteban. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahia Blanca; Argentina
Fil: Massheimer, Virginia Laura. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahia Blanca; Argentina
description Medroxyprogesterone acetate (MPA) is a synthetic progestin commonly used in hormone replacement therapy (HRT). The aim of this work was to study and compare the effect of progesterone (Pg) and MPA, on the regulation of cellular events associated with vascular homeostasis and disease. Platelet adhesion to endothelial cells (ECs), nitric oxide (NO) production, and cell migration were studied using murine endothelial cells in vitro exposed to the progestins. After seven minutes treatment, MPA significantly inhibited NO synthesis with respect to control value; meanwhile Pg markedly increased vasoactive production. In senile ECs, the stimulatory action of Pg decreases; meanwhile MPA maintained its ability to inhibit NO synthesis.The presence of RU486 antagonized each steroid action. When ECs were preincubated with PD98059 (MAPK inhibitor) or chelerythrine (PKC inhibitor) before Pg or MPA treatment, the former totally suppressed the steroid action, but the PKC antagonist did not affect NO production. In the presence of a PI3K inhibitor (LY294002) a partial reduction in Pg effect, and a reversal of MPA action was detected. Using indomethacin the contribution of cyclooxygenase (COX) pathway was also detected. On platelet adhesion assays, Pg inhibited and MPA stimulated platelet adhesion to ECs. Under inflammatory conditions, Pg prevented platelet adhesion induced by lipopolysaccharide (LPS); meanwhile MPA potentiated the stimulatory action of LPS. Finally, although both steroids enhanced ECs migration, MPA exhibited a greater effect. In conclusion the data presented in this research provide evidence of a differential regulation of vascular function by Pg and MPA
publishDate 2013
dc.date.none.fl_str_mv 2013-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/7452
Cutini, Pablo Hernan; Campelo, Adrián Esteban; Massheimer, Virginia Laura; Differential regulation of endothelium behavior by progesterone and medroxyprogesterone acetate; Bioscientifica; Journal Of Endocrinology; 220; 12-2013; 179-193
0022-0795
url http://hdl.handle.net/11336/7452
identifier_str_mv Cutini, Pablo Hernan; Campelo, Adrián Esteban; Massheimer, Virginia Laura; Differential regulation of endothelium behavior by progesterone and medroxyprogesterone acetate; Bioscientifica; Journal Of Endocrinology; 220; 12-2013; 179-193
0022-0795
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://joe.endocrinology-journals.org/content/220/3/179.long
info:eu-repo/semantics/altIdentifier/doi/10.1530/JOE-13-0263
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/rar
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Bioscientifica
publisher.none.fl_str_mv Bioscientifica
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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