A comprehensive analysis of gene expression alterations in newly synthesized Paspalum notatum autotetraploid

Autores
Martelotto, Luciano G.; Ortiz, Juan Pablo Amelio; Stein, Juliana; Espinoza, Francisco; Quarin, Camilo Luis; Pessino, Silvina Claudia
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Although the causes of novel variation in polyploids are not well understood, they could involve changes in gene expression through dosage-regulation, altered regulatory interactions and rapid genetic/epigenetic changes. The objective of this work was to contribute to the elucidation of the patterns and mechanisms of gene regulation during the early stages of polyploid formation. The genome-wide expression of ∼10,000 genes was monitored by using differential display transcript profiling on flowers of a diploid genotype and a newly-formed autotetraploid derivative. Differential expression of 64 clones was validated by reverse-Northern blot. Sequence analysis showed that 42 were homologous to 26 distinct genes of known function while the remaining 22 were classified as novel sequences. Genes detected were involved in processes of DNA repair, chromatin structure modification, regulation of transcription, proteolysis, protein folding, carbohydrate and lipid metabolism and signal transduction. Our results show evidence for gene expression alterations occurring immediately after polyploidization in Paspalum
Fil: Martelotto, Luciano G.. Universidad Catolica de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ortiz, Juan Pablo Amelio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Stein, Juliana. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Espinoza, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Quarin, Camilo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Pessino, Silvina Claudia. Universidad Nacional de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Autopolyploidy
Differential Display
Gene Expression Regulation
Paspalum Notatum
Ploidy-Regulated Expression
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/30520
Acceder
id CONICETDig_2c12271cb98b7bc93845a5173d66f4f5
oai_identifier_str oai:ri.conicet.gov.ar:11336/30520
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A comprehensive analysis of gene expression alterations in newly synthesized Paspalum notatum autotetraploidMartelotto, Luciano G.Ortiz, Juan Pablo AmelioStein, JulianaEspinoza, FranciscoQuarin, Camilo LuisPessino, Silvina ClaudiaAutopolyploidyDifferential DisplayGene Expression RegulationPaspalum NotatumPloidy-Regulated Expressionhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Although the causes of novel variation in polyploids are not well understood, they could involve changes in gene expression through dosage-regulation, altered regulatory interactions and rapid genetic/epigenetic changes. The objective of this work was to contribute to the elucidation of the patterns and mechanisms of gene regulation during the early stages of polyploid formation. The genome-wide expression of ∼10,000 genes was monitored by using differential display transcript profiling on flowers of a diploid genotype and a newly-formed autotetraploid derivative. Differential expression of 64 clones was validated by reverse-Northern blot. Sequence analysis showed that 42 were homologous to 26 distinct genes of known function while the remaining 22 were classified as novel sequences. Genes detected were involved in processes of DNA repair, chromatin structure modification, regulation of transcription, proteolysis, protein folding, carbohydrate and lipid metabolism and signal transduction. Our results show evidence for gene expression alterations occurring immediately after polyploidization in PaspalumFil: Martelotto, Luciano G.. Universidad Catolica de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ortiz, Juan Pablo Amelio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaFil: Stein, Juliana. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Espinoza, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Quarin, Camilo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Pessino, Silvina Claudia. Universidad Nacional de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier Ireland2005-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/30520Martelotto, Luciano G.; Ortiz, Juan Pablo Amelio; Stein, Juliana; Espinoza, Francisco; Quarin, Camilo Luis; et al.; A comprehensive analysis of gene expression alterations in newly synthesized Paspalum notatum autotetraploid; Elsevier Ireland; Plant Science; 169; 1; 12-2005; 211-2200168-9452CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.plantsci.2005.03.01info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0168945205001093info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:46:53Zoai:ri.conicet.gov.ar:11336/30520instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:46:53.96CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A comprehensive analysis of gene expression alterations in newly synthesized Paspalum notatum autotetraploid
title A comprehensive analysis of gene expression alterations in newly synthesized Paspalum notatum autotetraploid
spellingShingle A comprehensive analysis of gene expression alterations in newly synthesized Paspalum notatum autotetraploid
Martelotto, Luciano G.
Autopolyploidy
Differential Display
Gene Expression Regulation
Paspalum Notatum
Ploidy-Regulated Expression
title_short A comprehensive analysis of gene expression alterations in newly synthesized Paspalum notatum autotetraploid
title_full A comprehensive analysis of gene expression alterations in newly synthesized Paspalum notatum autotetraploid
title_fullStr A comprehensive analysis of gene expression alterations in newly synthesized Paspalum notatum autotetraploid
title_full_unstemmed A comprehensive analysis of gene expression alterations in newly synthesized Paspalum notatum autotetraploid
title_sort A comprehensive analysis of gene expression alterations in newly synthesized Paspalum notatum autotetraploid
dc.creator.none.fl_str_mv Martelotto, Luciano G.
Ortiz, Juan Pablo Amelio
Stein, Juliana
Espinoza, Francisco
Quarin, Camilo Luis
Pessino, Silvina Claudia
author Martelotto, Luciano G.
author_facet Martelotto, Luciano G.
Ortiz, Juan Pablo Amelio
Stein, Juliana
Espinoza, Francisco
Quarin, Camilo Luis
Pessino, Silvina Claudia
author_role author
author2 Ortiz, Juan Pablo Amelio
Stein, Juliana
Espinoza, Francisco
Quarin, Camilo Luis
Pessino, Silvina Claudia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Autopolyploidy
Differential Display
Gene Expression Regulation
Paspalum Notatum
Ploidy-Regulated Expression
topic Autopolyploidy
Differential Display
Gene Expression Regulation
Paspalum Notatum
Ploidy-Regulated Expression
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Although the causes of novel variation in polyploids are not well understood, they could involve changes in gene expression through dosage-regulation, altered regulatory interactions and rapid genetic/epigenetic changes. The objective of this work was to contribute to the elucidation of the patterns and mechanisms of gene regulation during the early stages of polyploid formation. The genome-wide expression of ∼10,000 genes was monitored by using differential display transcript profiling on flowers of a diploid genotype and a newly-formed autotetraploid derivative. Differential expression of 64 clones was validated by reverse-Northern blot. Sequence analysis showed that 42 were homologous to 26 distinct genes of known function while the remaining 22 were classified as novel sequences. Genes detected were involved in processes of DNA repair, chromatin structure modification, regulation of transcription, proteolysis, protein folding, carbohydrate and lipid metabolism and signal transduction. Our results show evidence for gene expression alterations occurring immediately after polyploidization in Paspalum
Fil: Martelotto, Luciano G.. Universidad Catolica de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ortiz, Juan Pablo Amelio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Stein, Juliana. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Espinoza, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Quarin, Camilo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Pessino, Silvina Claudia. Universidad Nacional de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Although the causes of novel variation in polyploids are not well understood, they could involve changes in gene expression through dosage-regulation, altered regulatory interactions and rapid genetic/epigenetic changes. The objective of this work was to contribute to the elucidation of the patterns and mechanisms of gene regulation during the early stages of polyploid formation. The genome-wide expression of ∼10,000 genes was monitored by using differential display transcript profiling on flowers of a diploid genotype and a newly-formed autotetraploid derivative. Differential expression of 64 clones was validated by reverse-Northern blot. Sequence analysis showed that 42 were homologous to 26 distinct genes of known function while the remaining 22 were classified as novel sequences. Genes detected were involved in processes of DNA repair, chromatin structure modification, regulation of transcription, proteolysis, protein folding, carbohydrate and lipid metabolism and signal transduction. Our results show evidence for gene expression alterations occurring immediately after polyploidization in Paspalum
publishDate 2005
dc.date.none.fl_str_mv 2005-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/30520
Martelotto, Luciano G.; Ortiz, Juan Pablo Amelio; Stein, Juliana; Espinoza, Francisco; Quarin, Camilo Luis; et al.; A comprehensive analysis of gene expression alterations in newly synthesized Paspalum notatum autotetraploid; Elsevier Ireland; Plant Science; 169; 1; 12-2005; 211-220
0168-9452
CONICET Digital
CONICET
url http://hdl.handle.net/11336/30520
identifier_str_mv Martelotto, Luciano G.; Ortiz, Juan Pablo Amelio; Stein, Juliana; Espinoza, Francisco; Quarin, Camilo Luis; et al.; A comprehensive analysis of gene expression alterations in newly synthesized Paspalum notatum autotetraploid; Elsevier Ireland; Plant Science; 169; 1; 12-2005; 211-220
0168-9452
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.plantsci.2005.03.01
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0168945205001093
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613462890643456
score 13.070432

Publicaciones similares