Molecular identification of three isolates of Trichoderma harzianum isolated from agricultural soils in Argentina, and their abilities to detoxify in vitro metsulfuron methyl

Autores
Vazquez, María Belén; Barrera, Viviana; Bianchinotti, Maria Virginia
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Metsulfuron methyl (MM) is a sulfonylurea herbicide used worldwide for the control of weeds in cereal crops. In a previous study, three Trichoderma strains (T5, T6, and T7) capable of using MM as a sole carbon and energy source were isolated. In this study, the three strains were identified as Trichoderma harzianum using genetic markers, and the transformation of MM by the T. harzianum strains was quantified using spectrophotometry. Solutions of different phytotoxic doses of MM were incubated with plugs of mycelia of the Trichoderma strains and the resulting mixtures were used to assess MM detoxification. The toxicity of the degradation products was tested with a bioassay using pre-germinated seeds of Lens culinaris Medik. and mycelia. Strain T7 was more efficient in transforming MM at higher concentrations than the T5 and T6 strains. In the bioassay, T5 showed the best performance at higher MM doses. We conclude that both T5 and T7 strains are promising for further studies regarding treatment or amelioration of MM contaminated soils.
Le metsulfuron méthyle (MM) est une sulfonylurée herbicide utilisée a` travers le monde pour contrôler les mauvaises herbes des champs de céréales. Dans une étude précédente, trois souches de Trichoderma (T5, T6 et T7) capables d'utiliser le MM comme unique source de carbone et d'énergie avaient été isolées. Dans l'étude actuelle, les trois souches ont attribuées a` Trichoderma harzianum par des marqueurs génétiques, et la transformation du MM par les souches de T. harzianum a été quantifiée par spectrophotométrie. Des solutions comprenant différentes doses phytotoxiques de MM ont été incubées avec des boulettes de mycélium des souches de Trichoderma et les mélanges résultants ont été utilisés afin d'évaluer la détoxication du MM. La toxicité des produits de dégradation a été testée par un dosage biologique a` l'aide de graines pré-germées de Lens culinaris Medik. et du mycélium. La souche T7 était plus efficace que les souches T5 et T6 dans la transformation du MM a` des concentrations plus élevées. Lors de l'essai biologique, la souche T5 affichait la meilleure performance a` des doses de MM plus élevées. Les auteurs concluent que les souches T5 et T7 sont prometteuses en vue d'études futures dans la perspective de traitement ou d'amélioration de sols contaminés au MM. [Traduit par la Rédaction]
Fil: Vazquez, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); Argentina
Fil: Barrera, Viviana. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Bianchinotti, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); Argentina. Universidad Nacional del Sur; Argentina
Materia
Bioassay
Detoxification
Metsulfuron Methyl
Spectrophotometric Quantification
Trichoderma
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/11590

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network_name_str CONICET Digital (CONICET)
spelling Molecular identification of three isolates of Trichoderma harzianum isolated from agricultural soils in Argentina, and their abilities to detoxify in vitro metsulfuron methylVazquez, María BelénBarrera, VivianaBianchinotti, Maria VirginiaBioassayDetoxificationMetsulfuron MethylSpectrophotometric QuantificationTrichodermahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Metsulfuron methyl (MM) is a sulfonylurea herbicide used worldwide for the control of weeds in cereal crops. In a previous study, three Trichoderma strains (T5, T6, and T7) capable of using MM as a sole carbon and energy source were isolated. In this study, the three strains were identified as Trichoderma harzianum using genetic markers, and the transformation of MM by the T. harzianum strains was quantified using spectrophotometry. Solutions of different phytotoxic doses of MM were incubated with plugs of mycelia of the Trichoderma strains and the resulting mixtures were used to assess MM detoxification. The toxicity of the degradation products was tested with a bioassay using pre-germinated seeds of Lens culinaris Medik. and mycelia. Strain T7 was more efficient in transforming MM at higher concentrations than the T5 and T6 strains. In the bioassay, T5 showed the best performance at higher MM doses. We conclude that both T5 and T7 strains are promising for further studies regarding treatment or amelioration of MM contaminated soils.Le metsulfuron méthyle (MM) est une sulfonylurée herbicide utilisée a` travers le monde pour contrôler les mauvaises herbes des champs de céréales. Dans une étude précédente, trois souches de Trichoderma (T5, T6 et T7) capables d'utiliser le MM comme unique source de carbone et d'énergie avaient été isolées. Dans l'étude actuelle, les trois souches ont attribuées a` Trichoderma harzianum par des marqueurs génétiques, et la transformation du MM par les souches de T. harzianum a été quantifiée par spectrophotométrie. Des solutions comprenant différentes doses phytotoxiques de MM ont été incubées avec des boulettes de mycélium des souches de Trichoderma et les mélanges résultants ont été utilisés afin d'évaluer la détoxication du MM. La toxicité des produits de dégradation a été testée par un dosage biologique a` l'aide de graines pré-germées de Lens culinaris Medik. et du mycélium. La souche T7 était plus efficace que les souches T5 et T6 dans la transformation du MM a` des concentrations plus élevées. Lors de l'essai biologique, la souche T5 affichait la meilleure performance a` des doses de MM plus élevées. Les auteurs concluent que les souches T5 et T7 sont prometteuses en vue d'études futures dans la perspective de traitement ou d'amélioration de sols contaminés au MM. [Traduit par la Rédaction]Fil: Vazquez, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); ArgentinaFil: Barrera, Viviana. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: Bianchinotti, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); Argentina. Universidad Nacional del Sur; ArgentinaCanadian Science Publishing2015-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/11590Vazquez, María Belén; Barrera, Viviana; Bianchinotti, Maria Virginia; Molecular identification of three isolates of Trichoderma harzianum isolated from agricultural soils in Argentina, and their abilities to detoxify in vitro metsulfuron methyl; Canadian Science Publishing; Botany; 93; 11; 11-2015; 793-8001916-27901916-2804enginfo:eu-repo/semantics/altIdentifier/url/http://www.nrcresearchpress.com/doi/10.1139/cjb-2015-0085#.WH_G5vnhCJAinfo:eu-repo/semantics/altIdentifier/doi/10.1139/cjb-2015-0085info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:54:42Zoai:ri.conicet.gov.ar:11336/11590instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:54:43.081CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Molecular identification of three isolates of Trichoderma harzianum isolated from agricultural soils in Argentina, and their abilities to detoxify in vitro metsulfuron methyl
title Molecular identification of three isolates of Trichoderma harzianum isolated from agricultural soils in Argentina, and their abilities to detoxify in vitro metsulfuron methyl
spellingShingle Molecular identification of three isolates of Trichoderma harzianum isolated from agricultural soils in Argentina, and their abilities to detoxify in vitro metsulfuron methyl
Vazquez, María Belén
Bioassay
Detoxification
Metsulfuron Methyl
Spectrophotometric Quantification
Trichoderma
title_short Molecular identification of three isolates of Trichoderma harzianum isolated from agricultural soils in Argentina, and their abilities to detoxify in vitro metsulfuron methyl
title_full Molecular identification of three isolates of Trichoderma harzianum isolated from agricultural soils in Argentina, and their abilities to detoxify in vitro metsulfuron methyl
title_fullStr Molecular identification of three isolates of Trichoderma harzianum isolated from agricultural soils in Argentina, and their abilities to detoxify in vitro metsulfuron methyl
title_full_unstemmed Molecular identification of three isolates of Trichoderma harzianum isolated from agricultural soils in Argentina, and their abilities to detoxify in vitro metsulfuron methyl
title_sort Molecular identification of three isolates of Trichoderma harzianum isolated from agricultural soils in Argentina, and their abilities to detoxify in vitro metsulfuron methyl
dc.creator.none.fl_str_mv Vazquez, María Belén
Barrera, Viviana
Bianchinotti, Maria Virginia
author Vazquez, María Belén
author_facet Vazquez, María Belén
Barrera, Viviana
Bianchinotti, Maria Virginia
author_role author
author2 Barrera, Viviana
Bianchinotti, Maria Virginia
author2_role author
author
dc.subject.none.fl_str_mv Bioassay
Detoxification
Metsulfuron Methyl
Spectrophotometric Quantification
Trichoderma
topic Bioassay
Detoxification
Metsulfuron Methyl
Spectrophotometric Quantification
Trichoderma
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Metsulfuron methyl (MM) is a sulfonylurea herbicide used worldwide for the control of weeds in cereal crops. In a previous study, three Trichoderma strains (T5, T6, and T7) capable of using MM as a sole carbon and energy source were isolated. In this study, the three strains were identified as Trichoderma harzianum using genetic markers, and the transformation of MM by the T. harzianum strains was quantified using spectrophotometry. Solutions of different phytotoxic doses of MM were incubated with plugs of mycelia of the Trichoderma strains and the resulting mixtures were used to assess MM detoxification. The toxicity of the degradation products was tested with a bioassay using pre-germinated seeds of Lens culinaris Medik. and mycelia. Strain T7 was more efficient in transforming MM at higher concentrations than the T5 and T6 strains. In the bioassay, T5 showed the best performance at higher MM doses. We conclude that both T5 and T7 strains are promising for further studies regarding treatment or amelioration of MM contaminated soils.
Le metsulfuron méthyle (MM) est une sulfonylurée herbicide utilisée a` travers le monde pour contrôler les mauvaises herbes des champs de céréales. Dans une étude précédente, trois souches de Trichoderma (T5, T6 et T7) capables d'utiliser le MM comme unique source de carbone et d'énergie avaient été isolées. Dans l'étude actuelle, les trois souches ont attribuées a` Trichoderma harzianum par des marqueurs génétiques, et la transformation du MM par les souches de T. harzianum a été quantifiée par spectrophotométrie. Des solutions comprenant différentes doses phytotoxiques de MM ont été incubées avec des boulettes de mycélium des souches de Trichoderma et les mélanges résultants ont été utilisés afin d'évaluer la détoxication du MM. La toxicité des produits de dégradation a été testée par un dosage biologique a` l'aide de graines pré-germées de Lens culinaris Medik. et du mycélium. La souche T7 était plus efficace que les souches T5 et T6 dans la transformation du MM a` des concentrations plus élevées. Lors de l'essai biologique, la souche T5 affichait la meilleure performance a` des doses de MM plus élevées. Les auteurs concluent que les souches T5 et T7 sont prometteuses en vue d'études futures dans la perspective de traitement ou d'amélioration de sols contaminés au MM. [Traduit par la Rédaction]
Fil: Vazquez, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); Argentina
Fil: Barrera, Viviana. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Bianchinotti, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); Argentina. Universidad Nacional del Sur; Argentina
description Metsulfuron methyl (MM) is a sulfonylurea herbicide used worldwide for the control of weeds in cereal crops. In a previous study, three Trichoderma strains (T5, T6, and T7) capable of using MM as a sole carbon and energy source were isolated. In this study, the three strains were identified as Trichoderma harzianum using genetic markers, and the transformation of MM by the T. harzianum strains was quantified using spectrophotometry. Solutions of different phytotoxic doses of MM were incubated with plugs of mycelia of the Trichoderma strains and the resulting mixtures were used to assess MM detoxification. The toxicity of the degradation products was tested with a bioassay using pre-germinated seeds of Lens culinaris Medik. and mycelia. Strain T7 was more efficient in transforming MM at higher concentrations than the T5 and T6 strains. In the bioassay, T5 showed the best performance at higher MM doses. We conclude that both T5 and T7 strains are promising for further studies regarding treatment or amelioration of MM contaminated soils.
publishDate 2015
dc.date.none.fl_str_mv 2015-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/11590
Vazquez, María Belén; Barrera, Viviana; Bianchinotti, Maria Virginia; Molecular identification of three isolates of Trichoderma harzianum isolated from agricultural soils in Argentina, and their abilities to detoxify in vitro metsulfuron methyl; Canadian Science Publishing; Botany; 93; 11; 11-2015; 793-800
1916-2790
1916-2804
url http://hdl.handle.net/11336/11590
identifier_str_mv Vazquez, María Belén; Barrera, Viviana; Bianchinotti, Maria Virginia; Molecular identification of three isolates of Trichoderma harzianum isolated from agricultural soils in Argentina, and their abilities to detoxify in vitro metsulfuron methyl; Canadian Science Publishing; Botany; 93; 11; 11-2015; 793-800
1916-2790
1916-2804
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/doi/10.1139/cjb-2015-0085
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dc.publisher.none.fl_str_mv Canadian Science Publishing
publisher.none.fl_str_mv Canadian Science Publishing
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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