Molecular identification of three isolates of Trichoderma harzianum isolated from agricultural soils in Argentina, and their abilities to detoxify in vitro metsulfuron methyl
- Autores
- Vazquez, María Belén; Barrera, Viviana; Bianchinotti, Maria Virginia
- Año de publicación
- 2015
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Metsulfuron methyl (MM) is a sulfonylurea herbicide used worldwide for the control of weeds in cereal crops. In a previous study, three Trichoderma strains (T5, T6, and T7) capable of using MM as a sole carbon and energy source were isolated. In this study, the three strains were identified as Trichoderma harzianum using genetic markers, and the transformation of MM by the T. harzianum strains was quantified using spectrophotometry. Solutions of different phytotoxic doses of MM were incubated with plugs of mycelia of the Trichoderma strains and the resulting mixtures were used to assess MM detoxification. The toxicity of the degradation products was tested with a bioassay using pre-germinated seeds of Lens culinaris Medik. and mycelia. Strain T7 was more efficient in transforming MM at higher concentrations than the T5 and T6 strains. In the bioassay, T5 showed the best performance at higher MM doses. We conclude that both T5 and T7 strains are promising for further studies regarding treatment or amelioration of MM contaminated soils.
Le metsulfuron méthyle (MM) est une sulfonylurée herbicide utilisée a` travers le monde pour contrôler les mauvaises herbes des champs de céréales. Dans une étude précédente, trois souches de Trichoderma (T5, T6 et T7) capables d'utiliser le MM comme unique source de carbone et d'énergie avaient été isolées. Dans l'étude actuelle, les trois souches ont attribuées a` Trichoderma harzianum par des marqueurs génétiques, et la transformation du MM par les souches de T. harzianum a été quantifiée par spectrophotométrie. Des solutions comprenant différentes doses phytotoxiques de MM ont été incubées avec des boulettes de mycélium des souches de Trichoderma et les mélanges résultants ont été utilisés afin d'évaluer la détoxication du MM. La toxicité des produits de dégradation a été testée par un dosage biologique a` l'aide de graines pré-germées de Lens culinaris Medik. et du mycélium. La souche T7 était plus efficace que les souches T5 et T6 dans la transformation du MM a` des concentrations plus élevées. Lors de l'essai biologique, la souche T5 affichait la meilleure performance a` des doses de MM plus élevées. Les auteurs concluent que les souches T5 et T7 sont prometteuses en vue d'études futures dans la perspective de traitement ou d'amélioration de sols contaminés au MM. [Traduit par la Rédaction]
Fil: Vazquez, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); Argentina
Fil: Barrera, Viviana. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Bianchinotti, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); Argentina. Universidad Nacional del Sur; Argentina - Materia
-
Bioassay
Detoxification
Metsulfuron Methyl
Spectrophotometric Quantification
Trichoderma - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/11590
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Molecular identification of three isolates of Trichoderma harzianum isolated from agricultural soils in Argentina, and their abilities to detoxify in vitro metsulfuron methylVazquez, María BelénBarrera, VivianaBianchinotti, Maria VirginiaBioassayDetoxificationMetsulfuron MethylSpectrophotometric QuantificationTrichodermahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Metsulfuron methyl (MM) is a sulfonylurea herbicide used worldwide for the control of weeds in cereal crops. In a previous study, three Trichoderma strains (T5, T6, and T7) capable of using MM as a sole carbon and energy source were isolated. In this study, the three strains were identified as Trichoderma harzianum using genetic markers, and the transformation of MM by the T. harzianum strains was quantified using spectrophotometry. Solutions of different phytotoxic doses of MM were incubated with plugs of mycelia of the Trichoderma strains and the resulting mixtures were used to assess MM detoxification. The toxicity of the degradation products was tested with a bioassay using pre-germinated seeds of Lens culinaris Medik. and mycelia. Strain T7 was more efficient in transforming MM at higher concentrations than the T5 and T6 strains. In the bioassay, T5 showed the best performance at higher MM doses. We conclude that both T5 and T7 strains are promising for further studies regarding treatment or amelioration of MM contaminated soils.Le metsulfuron méthyle (MM) est une sulfonylurée herbicide utilisée a` travers le monde pour contrôler les mauvaises herbes des champs de céréales. Dans une étude précédente, trois souches de Trichoderma (T5, T6 et T7) capables d'utiliser le MM comme unique source de carbone et d'énergie avaient été isolées. Dans l'étude actuelle, les trois souches ont attribuées a` Trichoderma harzianum par des marqueurs génétiques, et la transformation du MM par les souches de T. harzianum a été quantifiée par spectrophotométrie. Des solutions comprenant différentes doses phytotoxiques de MM ont été incubées avec des boulettes de mycélium des souches de Trichoderma et les mélanges résultants ont été utilisés afin d'évaluer la détoxication du MM. La toxicité des produits de dégradation a été testée par un dosage biologique a` l'aide de graines pré-germées de Lens culinaris Medik. et du mycélium. La souche T7 était plus efficace que les souches T5 et T6 dans la transformation du MM a` des concentrations plus élevées. Lors de l'essai biologique, la souche T5 affichait la meilleure performance a` des doses de MM plus élevées. Les auteurs concluent que les souches T5 et T7 sont prometteuses en vue d'études futures dans la perspective de traitement ou d'amélioration de sols contaminés au MM. [Traduit par la Rédaction]Fil: Vazquez, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); ArgentinaFil: Barrera, Viviana. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: Bianchinotti, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); Argentina. Universidad Nacional del Sur; ArgentinaCanadian Science Publishing2015-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/11590Vazquez, María Belén; Barrera, Viviana; Bianchinotti, Maria Virginia; Molecular identification of three isolates of Trichoderma harzianum isolated from agricultural soils in Argentina, and their abilities to detoxify in vitro metsulfuron methyl; Canadian Science Publishing; Botany; 93; 11; 11-2015; 793-8001916-27901916-2804enginfo:eu-repo/semantics/altIdentifier/url/http://www.nrcresearchpress.com/doi/10.1139/cjb-2015-0085#.WH_G5vnhCJAinfo:eu-repo/semantics/altIdentifier/doi/10.1139/cjb-2015-0085info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:54:42Zoai:ri.conicet.gov.ar:11336/11590instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:54:43.081CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Molecular identification of three isolates of Trichoderma harzianum isolated from agricultural soils in Argentina, and their abilities to detoxify in vitro metsulfuron methyl |
title |
Molecular identification of three isolates of Trichoderma harzianum isolated from agricultural soils in Argentina, and their abilities to detoxify in vitro metsulfuron methyl |
spellingShingle |
Molecular identification of three isolates of Trichoderma harzianum isolated from agricultural soils in Argentina, and their abilities to detoxify in vitro metsulfuron methyl Vazquez, María Belén Bioassay Detoxification Metsulfuron Methyl Spectrophotometric Quantification Trichoderma |
title_short |
Molecular identification of three isolates of Trichoderma harzianum isolated from agricultural soils in Argentina, and their abilities to detoxify in vitro metsulfuron methyl |
title_full |
Molecular identification of three isolates of Trichoderma harzianum isolated from agricultural soils in Argentina, and their abilities to detoxify in vitro metsulfuron methyl |
title_fullStr |
Molecular identification of three isolates of Trichoderma harzianum isolated from agricultural soils in Argentina, and their abilities to detoxify in vitro metsulfuron methyl |
title_full_unstemmed |
Molecular identification of three isolates of Trichoderma harzianum isolated from agricultural soils in Argentina, and their abilities to detoxify in vitro metsulfuron methyl |
title_sort |
Molecular identification of three isolates of Trichoderma harzianum isolated from agricultural soils in Argentina, and their abilities to detoxify in vitro metsulfuron methyl |
dc.creator.none.fl_str_mv |
Vazquez, María Belén Barrera, Viviana Bianchinotti, Maria Virginia |
author |
Vazquez, María Belén |
author_facet |
Vazquez, María Belén Barrera, Viviana Bianchinotti, Maria Virginia |
author_role |
author |
author2 |
Barrera, Viviana Bianchinotti, Maria Virginia |
author2_role |
author author |
dc.subject.none.fl_str_mv |
Bioassay Detoxification Metsulfuron Methyl Spectrophotometric Quantification Trichoderma |
topic |
Bioassay Detoxification Metsulfuron Methyl Spectrophotometric Quantification Trichoderma |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Metsulfuron methyl (MM) is a sulfonylurea herbicide used worldwide for the control of weeds in cereal crops. In a previous study, three Trichoderma strains (T5, T6, and T7) capable of using MM as a sole carbon and energy source were isolated. In this study, the three strains were identified as Trichoderma harzianum using genetic markers, and the transformation of MM by the T. harzianum strains was quantified using spectrophotometry. Solutions of different phytotoxic doses of MM were incubated with plugs of mycelia of the Trichoderma strains and the resulting mixtures were used to assess MM detoxification. The toxicity of the degradation products was tested with a bioassay using pre-germinated seeds of Lens culinaris Medik. and mycelia. Strain T7 was more efficient in transforming MM at higher concentrations than the T5 and T6 strains. In the bioassay, T5 showed the best performance at higher MM doses. We conclude that both T5 and T7 strains are promising for further studies regarding treatment or amelioration of MM contaminated soils. Le metsulfuron méthyle (MM) est une sulfonylurée herbicide utilisée a` travers le monde pour contrôler les mauvaises herbes des champs de céréales. Dans une étude précédente, trois souches de Trichoderma (T5, T6 et T7) capables d'utiliser le MM comme unique source de carbone et d'énergie avaient été isolées. Dans l'étude actuelle, les trois souches ont attribuées a` Trichoderma harzianum par des marqueurs génétiques, et la transformation du MM par les souches de T. harzianum a été quantifiée par spectrophotométrie. Des solutions comprenant différentes doses phytotoxiques de MM ont été incubées avec des boulettes de mycélium des souches de Trichoderma et les mélanges résultants ont été utilisés afin d'évaluer la détoxication du MM. La toxicité des produits de dégradation a été testée par un dosage biologique a` l'aide de graines pré-germées de Lens culinaris Medik. et du mycélium. La souche T7 était plus efficace que les souches T5 et T6 dans la transformation du MM a` des concentrations plus élevées. Lors de l'essai biologique, la souche T5 affichait la meilleure performance a` des doses de MM plus élevées. Les auteurs concluent que les souches T5 et T7 sont prometteuses en vue d'études futures dans la perspective de traitement ou d'amélioration de sols contaminés au MM. [Traduit par la Rédaction] Fil: Vazquez, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); Argentina Fil: Barrera, Viviana. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias. Centro de Investigación de Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Bianchinotti, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida(i); Argentina. Universidad Nacional del Sur; Argentina |
description |
Metsulfuron methyl (MM) is a sulfonylurea herbicide used worldwide for the control of weeds in cereal crops. In a previous study, three Trichoderma strains (T5, T6, and T7) capable of using MM as a sole carbon and energy source were isolated. In this study, the three strains were identified as Trichoderma harzianum using genetic markers, and the transformation of MM by the T. harzianum strains was quantified using spectrophotometry. Solutions of different phytotoxic doses of MM were incubated with plugs of mycelia of the Trichoderma strains and the resulting mixtures were used to assess MM detoxification. The toxicity of the degradation products was tested with a bioassay using pre-germinated seeds of Lens culinaris Medik. and mycelia. Strain T7 was more efficient in transforming MM at higher concentrations than the T5 and T6 strains. In the bioassay, T5 showed the best performance at higher MM doses. We conclude that both T5 and T7 strains are promising for further studies regarding treatment or amelioration of MM contaminated soils. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-11 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/11590 Vazquez, María Belén; Barrera, Viviana; Bianchinotti, Maria Virginia; Molecular identification of three isolates of Trichoderma harzianum isolated from agricultural soils in Argentina, and their abilities to detoxify in vitro metsulfuron methyl; Canadian Science Publishing; Botany; 93; 11; 11-2015; 793-800 1916-2790 1916-2804 |
url |
http://hdl.handle.net/11336/11590 |
identifier_str_mv |
Vazquez, María Belén; Barrera, Viviana; Bianchinotti, Maria Virginia; Molecular identification of three isolates of Trichoderma harzianum isolated from agricultural soils in Argentina, and their abilities to detoxify in vitro metsulfuron methyl; Canadian Science Publishing; Botany; 93; 11; 11-2015; 793-800 1916-2790 1916-2804 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.nrcresearchpress.com/doi/10.1139/cjb-2015-0085#.WH_G5vnhCJA info:eu-repo/semantics/altIdentifier/doi/10.1139/cjb-2015-0085 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Canadian Science Publishing |
publisher.none.fl_str_mv |
Canadian Science Publishing |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.13397 |