Expanding the spectrum: Modified bacteriocin nisin as a Trojan horse against Gram-negative SalmonellaTyphimurium and Escherichia coli

Autores
Lanza, Lucía; Masias, Ruth Emilse; Chalon, Miriam Carolina; Cattaneo, Mauricio; Bellomio, Augusto
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Nisin is an antimicrobial peptide widely used as a biopreservative to extend shelf life and control foodborne pathogens, particularly Gram-positive bacteria such as Listeria monocytogenes. However, its efficacy against Gram-negative bacteria is limited due to its inability to penetrate their outer membrane and reach its target, lipid II, in the inner membrane. Agents that alter the outer membrane’s permeability can overcome this limitation, enabling nisin to access the periplasm and expand its antimicrobial spectrum. In this study, we aimed to extend nisin’s spectrum of action towards Gram-negative bacteria by fusing it with salmochelin, a siderophore produced by Salmonella.Siderophores capture extracellular iron and enter cells through specific receptors. The nisin-salmochelin conjugate was synthesized through an external chemical reaction, yielding a compound with activity against Gram-positive and Gram-negative bacteria. Among the Gram-negative bacteria, notable strains include S. enterica Newport, S. entericaTyphimurium, E. coli O157, E. coli EC3 (uropathogenic isolate), and Providencia rettgeri. This novel conjugate exhibits broad-spectrum activity against foodborne pathogens, significantly enhancing nisin’s potential as a food biopreservative and expanding its clinical applications.
Fil: Lanza, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Masias, Ruth Emilse. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Chalon, Miriam Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Cattaneo, Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Bellomio, Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Materia
SIDEROPHORE
BACTERIOSIN
MULTI-RESISTANCE
TROJAN HORSE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/263671
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/263671
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Expanding the spectrum: Modified bacteriocin nisin as a Trojan horse against Gram-negative SalmonellaTyphimurium and Escherichia coliLanza, LucíaMasias, Ruth EmilseChalon, Miriam CarolinaCattaneo, MauricioBellomio, AugustoSIDEROPHOREBACTERIOSINMULTI-RESISTANCETROJAN HORSEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Nisin is an antimicrobial peptide widely used as a biopreservative to extend shelf life and control foodborne pathogens, particularly Gram-positive bacteria such as Listeria monocytogenes. However, its efficacy against Gram-negative bacteria is limited due to its inability to penetrate their outer membrane and reach its target, lipid II, in the inner membrane. Agents that alter the outer membrane’s permeability can overcome this limitation, enabling nisin to access the periplasm and expand its antimicrobial spectrum. In this study, we aimed to extend nisin’s spectrum of action towards Gram-negative bacteria by fusing it with salmochelin, a siderophore produced by Salmonella.Siderophores capture extracellular iron and enter cells through specific receptors. The nisin-salmochelin conjugate was synthesized through an external chemical reaction, yielding a compound with activity against Gram-positive and Gram-negative bacteria. Among the Gram-negative bacteria, notable strains include S. enterica Newport, S. entericaTyphimurium, E. coli O157, E. coli EC3 (uropathogenic isolate), and Providencia rettgeri. This novel conjugate exhibits broad-spectrum activity against foodborne pathogens, significantly enhancing nisin’s potential as a food biopreservative and expanding its clinical applications.Fil: Lanza, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Masias, Ruth Emilse. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Chalon, Miriam Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Cattaneo, Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; ArgentinaFil: Bellomio, Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaWiley-VCH2025-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/263671Lanza, Lucía; Masias, Ruth Emilse; Chalon, Miriam Carolina; Cattaneo, Mauricio; Bellomio, Augusto; Expanding the spectrum: Modified bacteriocin nisin as a Trojan horse against Gram-negative SalmonellaTyphimurium and Escherichia coli; Wiley-VCH; Biotechnology Journal; 4-2025; 1-181860-67681860-7314CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.22541/au.173113412.28706263/v1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:01:36Zoai:ri.conicet.gov.ar:11336/263671instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:01:37.021CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Expanding the spectrum: Modified bacteriocin nisin as a Trojan horse against Gram-negative SalmonellaTyphimurium and Escherichia coli
title Expanding the spectrum: Modified bacteriocin nisin as a Trojan horse against Gram-negative SalmonellaTyphimurium and Escherichia coli
spellingShingle Expanding the spectrum: Modified bacteriocin nisin as a Trojan horse against Gram-negative SalmonellaTyphimurium and Escherichia coli
Lanza, Lucía
SIDEROPHORE
BACTERIOSIN
MULTI-RESISTANCE
TROJAN HORSE
title_short Expanding the spectrum: Modified bacteriocin nisin as a Trojan horse against Gram-negative SalmonellaTyphimurium and Escherichia coli
title_full Expanding the spectrum: Modified bacteriocin nisin as a Trojan horse against Gram-negative SalmonellaTyphimurium and Escherichia coli
title_fullStr Expanding the spectrum: Modified bacteriocin nisin as a Trojan horse against Gram-negative SalmonellaTyphimurium and Escherichia coli
title_full_unstemmed Expanding the spectrum: Modified bacteriocin nisin as a Trojan horse against Gram-negative SalmonellaTyphimurium and Escherichia coli
title_sort Expanding the spectrum: Modified bacteriocin nisin as a Trojan horse against Gram-negative SalmonellaTyphimurium and Escherichia coli
dc.creator.none.fl_str_mv Lanza, Lucía
Masias, Ruth Emilse
Chalon, Miriam Carolina
Cattaneo, Mauricio
Bellomio, Augusto
author Lanza, Lucía
author_facet Lanza, Lucía
Masias, Ruth Emilse
Chalon, Miriam Carolina
Cattaneo, Mauricio
Bellomio, Augusto
author_role author
author2 Masias, Ruth Emilse
Chalon, Miriam Carolina
Cattaneo, Mauricio
Bellomio, Augusto
author2_role author
author
author
author
dc.subject.none.fl_str_mv SIDEROPHORE
BACTERIOSIN
MULTI-RESISTANCE
TROJAN HORSE
topic SIDEROPHORE
BACTERIOSIN
MULTI-RESISTANCE
TROJAN HORSE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Nisin is an antimicrobial peptide widely used as a biopreservative to extend shelf life and control foodborne pathogens, particularly Gram-positive bacteria such as Listeria monocytogenes. However, its efficacy against Gram-negative bacteria is limited due to its inability to penetrate their outer membrane and reach its target, lipid II, in the inner membrane. Agents that alter the outer membrane’s permeability can overcome this limitation, enabling nisin to access the periplasm and expand its antimicrobial spectrum. In this study, we aimed to extend nisin’s spectrum of action towards Gram-negative bacteria by fusing it with salmochelin, a siderophore produced by Salmonella.Siderophores capture extracellular iron and enter cells through specific receptors. The nisin-salmochelin conjugate was synthesized through an external chemical reaction, yielding a compound with activity against Gram-positive and Gram-negative bacteria. Among the Gram-negative bacteria, notable strains include S. enterica Newport, S. entericaTyphimurium, E. coli O157, E. coli EC3 (uropathogenic isolate), and Providencia rettgeri. This novel conjugate exhibits broad-spectrum activity against foodborne pathogens, significantly enhancing nisin’s potential as a food biopreservative and expanding its clinical applications.
Fil: Lanza, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Masias, Ruth Emilse. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Chalon, Miriam Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Cattaneo, Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Bellomio, Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
description Nisin is an antimicrobial peptide widely used as a biopreservative to extend shelf life and control foodborne pathogens, particularly Gram-positive bacteria such as Listeria monocytogenes. However, its efficacy against Gram-negative bacteria is limited due to its inability to penetrate their outer membrane and reach its target, lipid II, in the inner membrane. Agents that alter the outer membrane’s permeability can overcome this limitation, enabling nisin to access the periplasm and expand its antimicrobial spectrum. In this study, we aimed to extend nisin’s spectrum of action towards Gram-negative bacteria by fusing it with salmochelin, a siderophore produced by Salmonella.Siderophores capture extracellular iron and enter cells through specific receptors. The nisin-salmochelin conjugate was synthesized through an external chemical reaction, yielding a compound with activity against Gram-positive and Gram-negative bacteria. Among the Gram-negative bacteria, notable strains include S. enterica Newport, S. entericaTyphimurium, E. coli O157, E. coli EC3 (uropathogenic isolate), and Providencia rettgeri. This novel conjugate exhibits broad-spectrum activity against foodborne pathogens, significantly enhancing nisin’s potential as a food biopreservative and expanding its clinical applications.
publishDate 2025
dc.date.none.fl_str_mv 2025-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/263671
Lanza, Lucía; Masias, Ruth Emilse; Chalon, Miriam Carolina; Cattaneo, Mauricio; Bellomio, Augusto; Expanding the spectrum: Modified bacteriocin nisin as a Trojan horse against Gram-negative SalmonellaTyphimurium and Escherichia coli; Wiley-VCH; Biotechnology Journal; 4-2025; 1-18
1860-6768
1860-7314
CONICET Digital
CONICET
url http://hdl.handle.net/11336/263671
identifier_str_mv Lanza, Lucía; Masias, Ruth Emilse; Chalon, Miriam Carolina; Cattaneo, Mauricio; Bellomio, Augusto; Expanding the spectrum: Modified bacteriocin nisin as a Trojan horse against Gram-negative SalmonellaTyphimurium and Escherichia coli; Wiley-VCH; Biotechnology Journal; 4-2025; 1-18
1860-6768
1860-7314
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.22541/au.173113412.28706263/v1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley-VCH
publisher.none.fl_str_mv Wiley-VCH
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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