A device to record ultra-rapid cooling profiles

Autores
Juan de Paz, Leornardo; Graf, Daniel Adolfo; Scandizzi, Angel Luis; Guibert, Edgardo Elvio; Rodriguez, Joaquin Valentin
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This work deals with the construction and performance of a measuring system capable of estimating temperature at sufficiently high speed (up to 1000 samples/sec). Due to its simple design and the utilization of standard materials, it could serve to recording the cooling profile of ultra-rapid procedures. An immersion device was also developed with the purpose of normalize the penetration speed of the sample in the LN2. The device allows also the comparative analysis of different cooling profiles. The system consists of an immersion device of the sample in the cooling agent, a temperature measurement system developed by Kleihans F and a laptop computer. To test the system, we recorded the cooling profiles of 10 L of distilled water and 6 M glycerol solution, obtaining a cooling rate of 8732 °C.min-1 and 4441 °C.min-1 respectively. Also we determine a cooling rate of 204.012 °C.min-1 during the immersion of the thermocouple assembly in LN2. Although, the same device, with small technical modifications related to the handling of the sample, could be used to evaluate the recovery from LN2 temperature to room temperature (re-warming).
Fil: Juan de Paz, Leornardo. Universidad Nacional de Rosario. Secretaria de Ciencia y Tecnica. Centro Binacional de Investigación en Criobiologia Clinica y Aplicada; Argentina
Fil: Graf, Daniel Adolfo. Universidad Nacional de Rosario. Secretaria de Ciencia y Tecnica. Centro Binacional de Investigación en Criobiologia Clinica y Aplicada; Argentina
Fil: Scandizzi, Angel Luis. Universidad Nacional de Rosario. Secretaria de Ciencia y Tecnica. Centro Binacional de Investigación en Criobiologia Clinica y Aplicada; Argentina
Fil: Guibert, Edgardo Elvio. Universidad Nacional de Rosario. Secretaria de Ciencia y Tecnica. Centro Binacional de Investigación en Criobiologia Clinica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rodriguez, Joaquin Valentin. Universidad Nacional de Rosario. Secretaria de Ciencia y Tecnica. Centro Binacional de Investigación en Criobiologia Clinica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Cooling
Vitrification
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/21966
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/21966
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A device to record ultra-rapid cooling profilesJuan de Paz, LeornardoGraf, Daniel AdolfoScandizzi, Angel LuisGuibert, Edgardo ElvioRodriguez, Joaquin ValentinCoolingVitrificationhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1This work deals with the construction and performance of a measuring system capable of estimating temperature at sufficiently high speed (up to 1000 samples/sec). Due to its simple design and the utilization of standard materials, it could serve to recording the cooling profile of ultra-rapid procedures. An immersion device was also developed with the purpose of normalize the penetration speed of the sample in the LN2. The device allows also the comparative analysis of different cooling profiles. The system consists of an immersion device of the sample in the cooling agent, a temperature measurement system developed by Kleihans F and a laptop computer. To test the system, we recorded the cooling profiles of 10 L of distilled water and 6 M glycerol solution, obtaining a cooling rate of 8732 °C.min-1 and 4441 °C.min-1 respectively. Also we determine a cooling rate of 204.012 °C.min-1 during the immersion of the thermocouple assembly in LN2. Although, the same device, with small technical modifications related to the handling of the sample, could be used to evaluate the recovery from LN2 temperature to room temperature (re-warming).Fil: Juan de Paz, Leornardo. Universidad Nacional de Rosario. Secretaria de Ciencia y Tecnica. Centro Binacional de Investigación en Criobiologia Clinica y Aplicada; ArgentinaFil: Graf, Daniel Adolfo. Universidad Nacional de Rosario. Secretaria de Ciencia y Tecnica. Centro Binacional de Investigación en Criobiologia Clinica y Aplicada; ArgentinaFil: Scandizzi, Angel Luis. Universidad Nacional de Rosario. Secretaria de Ciencia y Tecnica. Centro Binacional de Investigación en Criobiologia Clinica y Aplicada; ArgentinaFil: Guibert, Edgardo Elvio. Universidad Nacional de Rosario. Secretaria de Ciencia y Tecnica. Centro Binacional de Investigación en Criobiologia Clinica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rodriguez, Joaquin Valentin. Universidad Nacional de Rosario. Secretaria de Ciencia y Tecnica. Centro Binacional de Investigación en Criobiologia Clinica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaCryo Letters2013-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/21966Juan de Paz, Leornardo; Graf, Daniel Adolfo; Scandizzi, Angel Luis; Guibert, Edgardo Elvio; Rodriguez, Joaquin Valentin; A device to record ultra-rapid cooling profiles; Cryo Letters; Cryo-letters; 34; 4; 8-2013; 360-3680143-2044CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.cryoletters.org/Abstracts/vol_34_4_2013.htm#360info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:01:34Zoai:ri.conicet.gov.ar:11336/21966instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:01:34.309CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A device to record ultra-rapid cooling profiles
title A device to record ultra-rapid cooling profiles
spellingShingle A device to record ultra-rapid cooling profiles
Juan de Paz, Leornardo
Cooling
Vitrification
title_short A device to record ultra-rapid cooling profiles
title_full A device to record ultra-rapid cooling profiles
title_fullStr A device to record ultra-rapid cooling profiles
title_full_unstemmed A device to record ultra-rapid cooling profiles
title_sort A device to record ultra-rapid cooling profiles
dc.creator.none.fl_str_mv Juan de Paz, Leornardo
Graf, Daniel Adolfo
Scandizzi, Angel Luis
Guibert, Edgardo Elvio
Rodriguez, Joaquin Valentin
author Juan de Paz, Leornardo
author_facet Juan de Paz, Leornardo
Graf, Daniel Adolfo
Scandizzi, Angel Luis
Guibert, Edgardo Elvio
Rodriguez, Joaquin Valentin
author_role author
author2 Graf, Daniel Adolfo
Scandizzi, Angel Luis
Guibert, Edgardo Elvio
Rodriguez, Joaquin Valentin
author2_role author
author
author
author
dc.subject.none.fl_str_mv Cooling
Vitrification
topic Cooling
Vitrification
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv This work deals with the construction and performance of a measuring system capable of estimating temperature at sufficiently high speed (up to 1000 samples/sec). Due to its simple design and the utilization of standard materials, it could serve to recording the cooling profile of ultra-rapid procedures. An immersion device was also developed with the purpose of normalize the penetration speed of the sample in the LN2. The device allows also the comparative analysis of different cooling profiles. The system consists of an immersion device of the sample in the cooling agent, a temperature measurement system developed by Kleihans F and a laptop computer. To test the system, we recorded the cooling profiles of 10 L of distilled water and 6 M glycerol solution, obtaining a cooling rate of 8732 °C.min-1 and 4441 °C.min-1 respectively. Also we determine a cooling rate of 204.012 °C.min-1 during the immersion of the thermocouple assembly in LN2. Although, the same device, with small technical modifications related to the handling of the sample, could be used to evaluate the recovery from LN2 temperature to room temperature (re-warming).
Fil: Juan de Paz, Leornardo. Universidad Nacional de Rosario. Secretaria de Ciencia y Tecnica. Centro Binacional de Investigación en Criobiologia Clinica y Aplicada; Argentina
Fil: Graf, Daniel Adolfo. Universidad Nacional de Rosario. Secretaria de Ciencia y Tecnica. Centro Binacional de Investigación en Criobiologia Clinica y Aplicada; Argentina
Fil: Scandizzi, Angel Luis. Universidad Nacional de Rosario. Secretaria de Ciencia y Tecnica. Centro Binacional de Investigación en Criobiologia Clinica y Aplicada; Argentina
Fil: Guibert, Edgardo Elvio. Universidad Nacional de Rosario. Secretaria de Ciencia y Tecnica. Centro Binacional de Investigación en Criobiologia Clinica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rodriguez, Joaquin Valentin. Universidad Nacional de Rosario. Secretaria de Ciencia y Tecnica. Centro Binacional de Investigación en Criobiologia Clinica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description This work deals with the construction and performance of a measuring system capable of estimating temperature at sufficiently high speed (up to 1000 samples/sec). Due to its simple design and the utilization of standard materials, it could serve to recording the cooling profile of ultra-rapid procedures. An immersion device was also developed with the purpose of normalize the penetration speed of the sample in the LN2. The device allows also the comparative analysis of different cooling profiles. The system consists of an immersion device of the sample in the cooling agent, a temperature measurement system developed by Kleihans F and a laptop computer. To test the system, we recorded the cooling profiles of 10 L of distilled water and 6 M glycerol solution, obtaining a cooling rate of 8732 °C.min-1 and 4441 °C.min-1 respectively. Also we determine a cooling rate of 204.012 °C.min-1 during the immersion of the thermocouple assembly in LN2. Although, the same device, with small technical modifications related to the handling of the sample, could be used to evaluate the recovery from LN2 temperature to room temperature (re-warming).
publishDate 2013
dc.date.none.fl_str_mv 2013-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/21966
Juan de Paz, Leornardo; Graf, Daniel Adolfo; Scandizzi, Angel Luis; Guibert, Edgardo Elvio; Rodriguez, Joaquin Valentin; A device to record ultra-rapid cooling profiles; Cryo Letters; Cryo-letters; 34; 4; 8-2013; 360-368
0143-2044
CONICET Digital
CONICET
url http://hdl.handle.net/11336/21966
identifier_str_mv Juan de Paz, Leornardo; Graf, Daniel Adolfo; Scandizzi, Angel Luis; Guibert, Edgardo Elvio; Rodriguez, Joaquin Valentin; A device to record ultra-rapid cooling profiles; Cryo Letters; Cryo-letters; 34; 4; 8-2013; 360-368
0143-2044
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.cryoletters.org/Abstracts/vol_34_4_2013.htm#360
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cryo Letters
publisher.none.fl_str_mv Cryo Letters
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269704307081216
score 13.13397

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