Measurement of cooling and warming rates in vitrification-based plant cryopreservation protocols

Autores
Schneider Teixeira, Aline; Gonzalez Benito, M. Elena; Molina García, Antonio D.
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cryopreservation protocols include the use of additives and pre-treatments aimed to reduce the probability of ice nucleation at all temperatures, mainly through micro-viscosity increase. Still, there is a risk of ice formation in the temperature region comprised between the equilibrium freezing (Tf) and the glass transition (TG) temperatures. Consequently, fast cooling and warming, especially in this region, is a must to avoid ice-derived damage. Vitrification and droplet-vitrification techniques, frequently used cryopreservation protocols based in fast cooling, were studied, alongside with the corresponding warming procedures. A very fast data acquisition system, able to read very low temperatures, down to that of liquid nitrogen, was employed. Cooling rates, measured between -20 and -120 ºC, ranged from ca. 5 ºC s-1 to 400 ºC s-1, while warming rates spanned from ca. 2 ºC s-1 to 280 ºC s-1, for the different protocols and conditions studied. A wider measuring window (0 ºC to -150 ºC) produced lower rates for all cases. The cooling and warming rates were also related to the survival observed after the different procedures. Those protocols with the faster rates yielded the highest survival percentages.
Fil: Schneider Teixeira, Aline. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigaciones En Criotecnología de Alimentos (i); Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Instituto de Ciencia y Tecnología de Alimentos y Nutrición; España
Fil: Gonzalez Benito, M. Elena. Universidad Politecnica de Madrid; España
Fil: Molina García, Antonio D.. Consejo Superior de Investigaciones Científicas. Instituto de Ciencia y Tecnologia de Alimentos y Nutrición; España
Materia
Cooling
Warming
Rates
Vitrification
Droplet-vitrification
Plant
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/10655
Acceder
id CONICETDig_18eae6288ab70e4f7f73ef66f655c6cd
oai_identifier_str oai:ri.conicet.gov.ar:11336/10655
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Measurement of cooling and warming rates in vitrification-based plant cryopreservation protocolsSchneider Teixeira, AlineGonzalez Benito, M. ElenaMolina García, Antonio D.CoolingWarmingRatesVitrificationDroplet-vitrificationPlanthttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Cryopreservation protocols include the use of additives and pre-treatments aimed to reduce the probability of ice nucleation at all temperatures, mainly through micro-viscosity increase. Still, there is a risk of ice formation in the temperature region comprised between the equilibrium freezing (Tf) and the glass transition (TG) temperatures. Consequently, fast cooling and warming, especially in this region, is a must to avoid ice-derived damage. Vitrification and droplet-vitrification techniques, frequently used cryopreservation protocols based in fast cooling, were studied, alongside with the corresponding warming procedures. A very fast data acquisition system, able to read very low temperatures, down to that of liquid nitrogen, was employed. Cooling rates, measured between -20 and -120 ºC, ranged from ca. 5 ºC s-1 to 400 ºC s-1, while warming rates spanned from ca. 2 ºC s-1 to 280 ºC s-1, for the different protocols and conditions studied. A wider measuring window (0 ºC to -150 ºC) produced lower rates for all cases. The cooling and warming rates were also related to the survival observed after the different procedures. Those protocols with the faster rates yielded the highest survival percentages.Fil: Schneider Teixeira, Aline. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigaciones En Criotecnología de Alimentos (i); Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Instituto de Ciencia y Tecnología de Alimentos y Nutrición; EspañaFil: Gonzalez Benito, M. Elena. Universidad Politecnica de Madrid; EspañaFil: Molina García, Antonio D.. Consejo Superior de Investigaciones Científicas. Instituto de Ciencia y Tecnologia de Alimentos y Nutrición; EspañaAmerican Chemical Society2014-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/10655Schneider Teixeira, Aline; Gonzalez Benito, M. Elena; Molina García, Antonio D.; Measurement of cooling and warming rates in vitrification-based plant cryopreservation protocols; American Chemical Society; Biotechnology Progress; 30; 5; 8-2014; 1177–11848756-79381520-6033enginfo:eu-repo/semantics/altIdentifier/doi/10.1002/btpr.1938info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/btpr.1938/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:51:48Zoai:ri.conicet.gov.ar:11336/10655instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:51:48.304CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Measurement of cooling and warming rates in vitrification-based plant cryopreservation protocols
title Measurement of cooling and warming rates in vitrification-based plant cryopreservation protocols
spellingShingle Measurement of cooling and warming rates in vitrification-based plant cryopreservation protocols
Schneider Teixeira, Aline
Cooling
Warming
Rates
Vitrification
Droplet-vitrification
Plant
title_short Measurement of cooling and warming rates in vitrification-based plant cryopreservation protocols
title_full Measurement of cooling and warming rates in vitrification-based plant cryopreservation protocols
title_fullStr Measurement of cooling and warming rates in vitrification-based plant cryopreservation protocols
title_full_unstemmed Measurement of cooling and warming rates in vitrification-based plant cryopreservation protocols
title_sort Measurement of cooling and warming rates in vitrification-based plant cryopreservation protocols
dc.creator.none.fl_str_mv Schneider Teixeira, Aline
Gonzalez Benito, M. Elena
Molina García, Antonio D.
author Schneider Teixeira, Aline
author_facet Schneider Teixeira, Aline
Gonzalez Benito, M. Elena
Molina García, Antonio D.
author_role author
author2 Gonzalez Benito, M. Elena
Molina García, Antonio D.
author2_role author
author
dc.subject.none.fl_str_mv Cooling
Warming
Rates
Vitrification
Droplet-vitrification
Plant
topic Cooling
Warming
Rates
Vitrification
Droplet-vitrification
Plant
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Cryopreservation protocols include the use of additives and pre-treatments aimed to reduce the probability of ice nucleation at all temperatures, mainly through micro-viscosity increase. Still, there is a risk of ice formation in the temperature region comprised between the equilibrium freezing (Tf) and the glass transition (TG) temperatures. Consequently, fast cooling and warming, especially in this region, is a must to avoid ice-derived damage. Vitrification and droplet-vitrification techniques, frequently used cryopreservation protocols based in fast cooling, were studied, alongside with the corresponding warming procedures. A very fast data acquisition system, able to read very low temperatures, down to that of liquid nitrogen, was employed. Cooling rates, measured between -20 and -120 ºC, ranged from ca. 5 ºC s-1 to 400 ºC s-1, while warming rates spanned from ca. 2 ºC s-1 to 280 ºC s-1, for the different protocols and conditions studied. A wider measuring window (0 ºC to -150 ºC) produced lower rates for all cases. The cooling and warming rates were also related to the survival observed after the different procedures. Those protocols with the faster rates yielded the highest survival percentages.
Fil: Schneider Teixeira, Aline. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigaciones En Criotecnología de Alimentos (i); Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Instituto de Ciencia y Tecnología de Alimentos y Nutrición; España
Fil: Gonzalez Benito, M. Elena. Universidad Politecnica de Madrid; España
Fil: Molina García, Antonio D.. Consejo Superior de Investigaciones Científicas. Instituto de Ciencia y Tecnologia de Alimentos y Nutrición; España
description Cryopreservation protocols include the use of additives and pre-treatments aimed to reduce the probability of ice nucleation at all temperatures, mainly through micro-viscosity increase. Still, there is a risk of ice formation in the temperature region comprised between the equilibrium freezing (Tf) and the glass transition (TG) temperatures. Consequently, fast cooling and warming, especially in this region, is a must to avoid ice-derived damage. Vitrification and droplet-vitrification techniques, frequently used cryopreservation protocols based in fast cooling, were studied, alongside with the corresponding warming procedures. A very fast data acquisition system, able to read very low temperatures, down to that of liquid nitrogen, was employed. Cooling rates, measured between -20 and -120 ºC, ranged from ca. 5 ºC s-1 to 400 ºC s-1, while warming rates spanned from ca. 2 ºC s-1 to 280 ºC s-1, for the different protocols and conditions studied. A wider measuring window (0 ºC to -150 ºC) produced lower rates for all cases. The cooling and warming rates were also related to the survival observed after the different procedures. Those protocols with the faster rates yielded the highest survival percentages.
publishDate 2014
dc.date.none.fl_str_mv 2014-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/10655
Schneider Teixeira, Aline; Gonzalez Benito, M. Elena; Molina García, Antonio D.; Measurement of cooling and warming rates in vitrification-based plant cryopreservation protocols; American Chemical Society; Biotechnology Progress; 30; 5; 8-2014; 1177–1184
8756-7938
1520-6033
url http://hdl.handle.net/11336/10655
identifier_str_mv Schneider Teixeira, Aline; Gonzalez Benito, M. Elena; Molina García, Antonio D.; Measurement of cooling and warming rates in vitrification-based plant cryopreservation protocols; American Chemical Society; Biotechnology Progress; 30; 5; 8-2014; 1177–1184
8756-7938
1520-6033
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/btpr.1938
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/btpr.1938/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269117924507648
score 13.13397

Publicaciones similares