Enhancing the Functional Properties of Tea Tree Oil: In Vitro Antimicrobial Activity and Microencapsulation Strategy

Autores
Manzanelli, Franco Antonio; Ravetti, Soledad; Brignone, Sofía Gisella; Garro, Ariel Gustavo; Martinez, Sol Romina; Vallejo, Mariana Guadalupe; Palma, Santiago Daniel
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In the context of addressing antimicrobial drug resistance in periocular infections, Tea TreeOil (TTO) has emerged as a promising therapeutic option. This study aimed to assess the efficacy ofTTO against bacterial strains isolated from ocular infections, with a particular focus on its ability toinhibit biofilm formation. Additionally, we designed and analyzed microcapsules containing TTOto overcome certain unfavorable physicochemical properties and enhance its inherent biologicalattributes. The quality of TTO was confirmed through rigorous analysis using GC-MS and UV-Vistechniques. Our agar diffusion assay demonstrated the effectiveness of Tea Tree Oil (TTO) againstocular bacterial strains, including Corynebacterium spp., coagulase-negative Staphylococcus spp.,and Staphylococcus aureus, as well as a reference strain of Staphylococcus aureus (ATCC 25923). Notably, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC)for all tested microorganisms were found to be 0.2% and 0.4%, respectively, with the exception ofCorynebacterium spp., which exhibited resistance to TTO. Furthermore, TTO exhibited a substantial reduction in biofilm biomass, ranging from 30% to 70%, as determined by the MTT method.Through the spray-drying technique, we successfully prepared two TTO-containing formulationswith high encapsulation yields (80–85%), microencapsulation efficiency (90–95%), and embeddingrates (approximately 40%). These formulations yielded microcapsules with diameters of 6–12 µm,as determined by laser scattering particle size distribution analysis, and exhibited regular, sphericalmorphologies under scanning electron microscopy. Importantly, UV-Vis analysis post-encapsulationconfirmed the presence of TTO within the capsules, with preserved antioxidant and antimicrobialactivities. In summary, our findings underscore the substantial therapeutic potential of TTO and itsmicrocapsules for treating ocular infections.
Fil: Manzanelli, Franco Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones y Transferencia de Villa María. Universidad Nacional de Villa María. Centro de Investigaciones y Transferencia de Villa María; Argentina
Fil: Ravetti, Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones y Transferencia de Villa María. Universidad Nacional de Villa María. Centro de Investigaciones y Transferencia de Villa María; Argentina
Fil: Brignone, Sofía Gisella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina
Fil: Garro, Ariel Gustavo. Provincia de Córdoba. Ministerio de Ciencia y Técnica; Argentina
Fil: Martinez, Sol Romina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; Argentina
Fil: Vallejo, Mariana Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina
Fil: Palma, Santiago Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina
Materia
ESSENTIAL OILS
BIOLOGICAL ACTIVITY
ANTIMICROBIAL RESISTANCE
MICROCAPSULES
PERIOCULAR INFECTIONS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/222402
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/222402
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Enhancing the Functional Properties of Tea Tree Oil: In Vitro Antimicrobial Activity and Microencapsulation StrategyManzanelli, Franco AntonioRavetti, SoledadBrignone, Sofía GisellaGarro, Ariel GustavoMartinez, Sol RominaVallejo, Mariana GuadalupePalma, Santiago DanielESSENTIAL OILSBIOLOGICAL ACTIVITYANTIMICROBIAL RESISTANCEMICROCAPSULESPERIOCULAR INFECTIONShttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1In the context of addressing antimicrobial drug resistance in periocular infections, Tea TreeOil (TTO) has emerged as a promising therapeutic option. This study aimed to assess the efficacy ofTTO against bacterial strains isolated from ocular infections, with a particular focus on its ability toinhibit biofilm formation. Additionally, we designed and analyzed microcapsules containing TTOto overcome certain unfavorable physicochemical properties and enhance its inherent biologicalattributes. The quality of TTO was confirmed through rigorous analysis using GC-MS and UV-Vistechniques. Our agar diffusion assay demonstrated the effectiveness of Tea Tree Oil (TTO) againstocular bacterial strains, including Corynebacterium spp., coagulase-negative Staphylococcus spp.,and Staphylococcus aureus, as well as a reference strain of Staphylococcus aureus (ATCC 25923). Notably, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC)for all tested microorganisms were found to be 0.2% and 0.4%, respectively, with the exception ofCorynebacterium spp., which exhibited resistance to TTO. Furthermore, TTO exhibited a substantial reduction in biofilm biomass, ranging from 30% to 70%, as determined by the MTT method.Through the spray-drying technique, we successfully prepared two TTO-containing formulationswith high encapsulation yields (80–85%), microencapsulation efficiency (90–95%), and embeddingrates (approximately 40%). These formulations yielded microcapsules with diameters of 6–12 µm,as determined by laser scattering particle size distribution analysis, and exhibited regular, sphericalmorphologies under scanning electron microscopy. Importantly, UV-Vis analysis post-encapsulationconfirmed the presence of TTO within the capsules, with preserved antioxidant and antimicrobialactivities. In summary, our findings underscore the substantial therapeutic potential of TTO and itsmicrocapsules for treating ocular infections.Fil: Manzanelli, Franco Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones y Transferencia de Villa María. Universidad Nacional de Villa María. Centro de Investigaciones y Transferencia de Villa María; ArgentinaFil: Ravetti, Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones y Transferencia de Villa María. Universidad Nacional de Villa María. Centro de Investigaciones y Transferencia de Villa María; ArgentinaFil: Brignone, Sofía Gisella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; ArgentinaFil: Garro, Ariel Gustavo. Provincia de Córdoba. Ministerio de Ciencia y Técnica; ArgentinaFil: Martinez, Sol Romina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; ArgentinaFil: Vallejo, Mariana Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; ArgentinaFil: Palma, Santiago Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; ArgentinaMDPI2023-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/222402Manzanelli, Franco Antonio; Ravetti, Soledad; Brignone, Sofía Gisella; Garro, Ariel Gustavo; Martinez, Sol Romina; et al.; Enhancing the Functional Properties of Tea Tree Oil: In Vitro Antimicrobial Activity and Microencapsulation Strategy; MDPI; Pharmaceutics; 15; 10; 10-2023; 2489-25061999-4923CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1999-4923/15/10/2489info:eu-repo/semantics/altIdentifier/doi/10.3390/pharmaceutics15102489info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T10:59:28Zoai:ri.conicet.gov.ar:11336/222402instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 10:59:29.101CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Enhancing the Functional Properties of Tea Tree Oil: In Vitro Antimicrobial Activity and Microencapsulation Strategy
title Enhancing the Functional Properties of Tea Tree Oil: In Vitro Antimicrobial Activity and Microencapsulation Strategy
spellingShingle Enhancing the Functional Properties of Tea Tree Oil: In Vitro Antimicrobial Activity and Microencapsulation Strategy
Manzanelli, Franco Antonio
ESSENTIAL OILS
BIOLOGICAL ACTIVITY
ANTIMICROBIAL RESISTANCE
MICROCAPSULES
PERIOCULAR INFECTIONS
title_short Enhancing the Functional Properties of Tea Tree Oil: In Vitro Antimicrobial Activity and Microencapsulation Strategy
title_full Enhancing the Functional Properties of Tea Tree Oil: In Vitro Antimicrobial Activity and Microencapsulation Strategy
title_fullStr Enhancing the Functional Properties of Tea Tree Oil: In Vitro Antimicrobial Activity and Microencapsulation Strategy
title_full_unstemmed Enhancing the Functional Properties of Tea Tree Oil: In Vitro Antimicrobial Activity and Microencapsulation Strategy
title_sort Enhancing the Functional Properties of Tea Tree Oil: In Vitro Antimicrobial Activity and Microencapsulation Strategy
dc.creator.none.fl_str_mv Manzanelli, Franco Antonio
Ravetti, Soledad
Brignone, Sofía Gisella
Garro, Ariel Gustavo
Martinez, Sol Romina
Vallejo, Mariana Guadalupe
Palma, Santiago Daniel
author Manzanelli, Franco Antonio
author_facet Manzanelli, Franco Antonio
Ravetti, Soledad
Brignone, Sofía Gisella
Garro, Ariel Gustavo
Martinez, Sol Romina
Vallejo, Mariana Guadalupe
Palma, Santiago Daniel
author_role author
author2 Ravetti, Soledad
Brignone, Sofía Gisella
Garro, Ariel Gustavo
Martinez, Sol Romina
Vallejo, Mariana Guadalupe
Palma, Santiago Daniel
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ESSENTIAL OILS
BIOLOGICAL ACTIVITY
ANTIMICROBIAL RESISTANCE
MICROCAPSULES
PERIOCULAR INFECTIONS
topic ESSENTIAL OILS
BIOLOGICAL ACTIVITY
ANTIMICROBIAL RESISTANCE
MICROCAPSULES
PERIOCULAR INFECTIONS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In the context of addressing antimicrobial drug resistance in periocular infections, Tea TreeOil (TTO) has emerged as a promising therapeutic option. This study aimed to assess the efficacy ofTTO against bacterial strains isolated from ocular infections, with a particular focus on its ability toinhibit biofilm formation. Additionally, we designed and analyzed microcapsules containing TTOto overcome certain unfavorable physicochemical properties and enhance its inherent biologicalattributes. The quality of TTO was confirmed through rigorous analysis using GC-MS and UV-Vistechniques. Our agar diffusion assay demonstrated the effectiveness of Tea Tree Oil (TTO) againstocular bacterial strains, including Corynebacterium spp., coagulase-negative Staphylococcus spp.,and Staphylococcus aureus, as well as a reference strain of Staphylococcus aureus (ATCC 25923). Notably, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC)for all tested microorganisms were found to be 0.2% and 0.4%, respectively, with the exception ofCorynebacterium spp., which exhibited resistance to TTO. Furthermore, TTO exhibited a substantial reduction in biofilm biomass, ranging from 30% to 70%, as determined by the MTT method.Through the spray-drying technique, we successfully prepared two TTO-containing formulationswith high encapsulation yields (80–85%), microencapsulation efficiency (90–95%), and embeddingrates (approximately 40%). These formulations yielded microcapsules with diameters of 6–12 µm,as determined by laser scattering particle size distribution analysis, and exhibited regular, sphericalmorphologies under scanning electron microscopy. Importantly, UV-Vis analysis post-encapsulationconfirmed the presence of TTO within the capsules, with preserved antioxidant and antimicrobialactivities. In summary, our findings underscore the substantial therapeutic potential of TTO and itsmicrocapsules for treating ocular infections.
Fil: Manzanelli, Franco Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones y Transferencia de Villa María. Universidad Nacional de Villa María. Centro de Investigaciones y Transferencia de Villa María; Argentina
Fil: Ravetti, Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones y Transferencia de Villa María. Universidad Nacional de Villa María. Centro de Investigaciones y Transferencia de Villa María; Argentina
Fil: Brignone, Sofía Gisella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina
Fil: Garro, Ariel Gustavo. Provincia de Córdoba. Ministerio de Ciencia y Técnica; Argentina
Fil: Martinez, Sol Romina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; Argentina
Fil: Vallejo, Mariana Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina
Fil: Palma, Santiago Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina
description In the context of addressing antimicrobial drug resistance in periocular infections, Tea TreeOil (TTO) has emerged as a promising therapeutic option. This study aimed to assess the efficacy ofTTO against bacterial strains isolated from ocular infections, with a particular focus on its ability toinhibit biofilm formation. Additionally, we designed and analyzed microcapsules containing TTOto overcome certain unfavorable physicochemical properties and enhance its inherent biologicalattributes. The quality of TTO was confirmed through rigorous analysis using GC-MS and UV-Vistechniques. Our agar diffusion assay demonstrated the effectiveness of Tea Tree Oil (TTO) againstocular bacterial strains, including Corynebacterium spp., coagulase-negative Staphylococcus spp.,and Staphylococcus aureus, as well as a reference strain of Staphylococcus aureus (ATCC 25923). Notably, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC)for all tested microorganisms were found to be 0.2% and 0.4%, respectively, with the exception ofCorynebacterium spp., which exhibited resistance to TTO. Furthermore, TTO exhibited a substantial reduction in biofilm biomass, ranging from 30% to 70%, as determined by the MTT method.Through the spray-drying technique, we successfully prepared two TTO-containing formulationswith high encapsulation yields (80–85%), microencapsulation efficiency (90–95%), and embeddingrates (approximately 40%). These formulations yielded microcapsules with diameters of 6–12 µm,as determined by laser scattering particle size distribution analysis, and exhibited regular, sphericalmorphologies under scanning electron microscopy. Importantly, UV-Vis analysis post-encapsulationconfirmed the presence of TTO within the capsules, with preserved antioxidant and antimicrobialactivities. In summary, our findings underscore the substantial therapeutic potential of TTO and itsmicrocapsules for treating ocular infections.
publishDate 2023
dc.date.none.fl_str_mv 2023-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/222402
Manzanelli, Franco Antonio; Ravetti, Soledad; Brignone, Sofía Gisella; Garro, Ariel Gustavo; Martinez, Sol Romina; et al.; Enhancing the Functional Properties of Tea Tree Oil: In Vitro Antimicrobial Activity and Microencapsulation Strategy; MDPI; Pharmaceutics; 15; 10; 10-2023; 2489-2506
1999-4923
CONICET Digital
CONICET
url http://hdl.handle.net/11336/222402
identifier_str_mv Manzanelli, Franco Antonio; Ravetti, Soledad; Brignone, Sofía Gisella; Garro, Ariel Gustavo; Martinez, Sol Romina; et al.; Enhancing the Functional Properties of Tea Tree Oil: In Vitro Antimicrobial Activity and Microencapsulation Strategy; MDPI; Pharmaceutics; 15; 10; 10-2023; 2489-2506
1999-4923
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1999-4923/15/10/2489
info:eu-repo/semantics/altIdentifier/doi/10.3390/pharmaceutics15102489
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
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reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.000565

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