Integrated strategies for the control of Nacobbus aberrans s.l. in tomato

Autores
Sosa, Ana Laura; Girardi, Natalia Soledad; Rosso, Laura Cristina; Passone, Maria Alejandra
Año de publicación
2024
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Microorganisms in the tomato rhizosphere ecosystem develop associations, many of which canbe beneficial or pathogenic for this crop. The plant parasitic nematode (PPN) Nacobbus sp. cause significant economic losses to horticultural farmers in Argentina. The distribution of this genusis limited to the American continent and is consider a quarantine pest. Biological control strategies of PPN are environmentally friendly alternatives that ensure the sustainability of the horticultural system. The aim of this study was to evaluate the potential of the combined application of broccoli aqueous extract (BAE) and Purpureocillium lilacinum SR14 fungus in tomato plants (Solanumlycopersicum var. Platense) for the management of N. aberrans s.l. Three-week-old seedlings were transplanted into pots containing the following treatments: T1: control (sterile horticultural soil), T2: naturally infested horticultural soil (NIHS), T3: NIHS + SR14(1x10exp6 spores/g), T4:NIHS + BAE(12%), T5: SHNI + SR14(1x10exp6 spores/g) + BAE(12%). Six replicates per treatment were carried out and plants were maintained in a green house. Data, collected at 120 days post transplant,revealed that tomato growth was affected in NIHS(T2). Plants showed a 47% and 30% reduction in weight and length, respectively, compared to uninfected soil (T1). The highest number (4 tomatoes/plant) and weight mean (80 g) of fruits were recorded in T4. The fungus alone or combined with BAE (T3, T4 and T5) effectively controlled N. aberrans s.l. population (reduction percentages: galls = 35, 90 and 93%, egg masses = 5,94 and 90% and reproduction factor = 90, 93 and 98%). Physicochemical and biological analyzes were also performed to evaluate the effect of the treatment on the soil ecosystem. The data showed an increase in organic matter for all treatments compared to the initial condition. Nutrient values such as P, N and Swere maintained throughout the trial. Regarding the soil mycobiota, the fungal count (cfu/g) was around 106 for all treatments, but a lower diversity was observed in T5 with a prevalence of P. lilacinum (3x10exp4). As regards the nematofauna, treatments did not significantly influence the number of individuals (n°/g of soil) belonging to the different trophic groups (phytopathogens:16, bacteriophages: 19, predators: 10 and fungivores: 15), with the exception of T4, where ahigher count of omnivores was observed (30). Finally, studies on microbial activity revealed that treatments did not influence the counts (MPN/g) of ammonifying (mean: 1.9x10exp2) and denitrifying agents (mean: 2.7x1013), while T4 and T5 reduced the density of 1 log unit of nitrate- reducing bacteria (mean: 3.4x10exp6) and aerobic-heterotrophic bacteria (mean: 3.9x10exp8). In conclusion, the integrated strategy reveal a synergistic effect for the control of N. aberrans s.l.,without affecting tomato plant development, nutrient content and soil microbiome.
Fil: Sosa, Ana Laura. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Ecología Microbiana; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
Fil: Girardi, Natalia Soledad. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Ecología Microbiana; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rosso, Laura Cristina. Istituto Per La Protezione Sostenibile Delle Piante; Italia
Fil: Passone, Maria Alejandra. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
35th Symposium of the European Society of Nematologists
Cordoba
España
European Society of Nematologists
Consejo Superior de Investigaciones Científicas
Instituto Andaluz de Investigación y Formación Agraria, Pesquera, Alimentaria y de la Producción Ecológica
Materia
BIOCONTROL
NACOBBUS ABERRANS
TOMATO
PURPUREOCILLUM LILACINUM
BOCCOLI EXTRACTS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/238304

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network_name_str CONICET Digital (CONICET)
spelling Integrated strategies for the control of Nacobbus aberrans s.l. in tomatoSosa, Ana LauraGirardi, Natalia SoledadRosso, Laura CristinaPassone, Maria AlejandraBIOCONTROLNACOBBUS ABERRANSTOMATOPURPUREOCILLUM LILACINUMBOCCOLI EXTRACTShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Microorganisms in the tomato rhizosphere ecosystem develop associations, many of which canbe beneficial or pathogenic for this crop. The plant parasitic nematode (PPN) Nacobbus sp. cause significant economic losses to horticultural farmers in Argentina. The distribution of this genusis limited to the American continent and is consider a quarantine pest. Biological control strategies of PPN are environmentally friendly alternatives that ensure the sustainability of the horticultural system. The aim of this study was to evaluate the potential of the combined application of broccoli aqueous extract (BAE) and Purpureocillium lilacinum SR14 fungus in tomato plants (Solanumlycopersicum var. Platense) for the management of N. aberrans s.l. Three-week-old seedlings were transplanted into pots containing the following treatments: T1: control (sterile horticultural soil), T2: naturally infested horticultural soil (NIHS), T3: NIHS + SR14(1x10exp6 spores/g), T4:NIHS + BAE(12%), T5: SHNI + SR14(1x10exp6 spores/g) + BAE(12%). Six replicates per treatment were carried out and plants were maintained in a green house. Data, collected at 120 days post transplant,revealed that tomato growth was affected in NIHS(T2). Plants showed a 47% and 30% reduction in weight and length, respectively, compared to uninfected soil (T1). The highest number (4 tomatoes/plant) and weight mean (80 g) of fruits were recorded in T4. The fungus alone or combined with BAE (T3, T4 and T5) effectively controlled N. aberrans s.l. population (reduction percentages: galls = 35, 90 and 93%, egg masses = 5,94 and 90% and reproduction factor = 90, 93 and 98%). Physicochemical and biological analyzes were also performed to evaluate the effect of the treatment on the soil ecosystem. The data showed an increase in organic matter for all treatments compared to the initial condition. Nutrient values such as P, N and Swere maintained throughout the trial. Regarding the soil mycobiota, the fungal count (cfu/g) was around 106 for all treatments, but a lower diversity was observed in T5 with a prevalence of P. lilacinum (3x10exp4). As regards the nematofauna, treatments did not significantly influence the number of individuals (n°/g of soil) belonging to the different trophic groups (phytopathogens:16, bacteriophages: 19, predators: 10 and fungivores: 15), with the exception of T4, where ahigher count of omnivores was observed (30). Finally, studies on microbial activity revealed that treatments did not influence the counts (MPN/g) of ammonifying (mean: 1.9x10exp2) and denitrifying agents (mean: 2.7x1013), while T4 and T5 reduced the density of 1 log unit of nitrate- reducing bacteria (mean: 3.4x10exp6) and aerobic-heterotrophic bacteria (mean: 3.9x10exp8). In conclusion, the integrated strategy reveal a synergistic effect for the control of N. aberrans s.l.,without affecting tomato plant development, nutrient content and soil microbiome.Fil: Sosa, Ana Laura. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Ecología Microbiana; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; ArgentinaFil: Girardi, Natalia Soledad. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Ecología Microbiana; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rosso, Laura Cristina. Istituto Per La Protezione Sostenibile Delle Piante; ItaliaFil: Passone, Maria Alejandra. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina35th Symposium of the European Society of NematologistsCordobaEspañaEuropean Society of NematologistsConsejo Superior de Investigaciones CientíficasInstituto Andaluz de Investigación y Formación Agraria, Pesquera, Alimentaria y de la Producción EcológicaEuropean Society of Nematologists2024info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectSimposioBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/238304Integrated strategies for the control of Nacobbus aberrans s.l. in tomato; 35th Symposium of the European Society of Nematologists; Cordoba; España; 2024; 290-291CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://esn24cordoba.com/Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:48:09Zoai:ri.conicet.gov.ar:11336/238304instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:48:09.771CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Integrated strategies for the control of Nacobbus aberrans s.l. in tomato
title Integrated strategies for the control of Nacobbus aberrans s.l. in tomato
spellingShingle Integrated strategies for the control of Nacobbus aberrans s.l. in tomato
Sosa, Ana Laura
BIOCONTROL
NACOBBUS ABERRANS
TOMATO
PURPUREOCILLUM LILACINUM
BOCCOLI EXTRACTS
title_short Integrated strategies for the control of Nacobbus aberrans s.l. in tomato
title_full Integrated strategies for the control of Nacobbus aberrans s.l. in tomato
title_fullStr Integrated strategies for the control of Nacobbus aberrans s.l. in tomato
title_full_unstemmed Integrated strategies for the control of Nacobbus aberrans s.l. in tomato
title_sort Integrated strategies for the control of Nacobbus aberrans s.l. in tomato
dc.creator.none.fl_str_mv Sosa, Ana Laura
Girardi, Natalia Soledad
Rosso, Laura Cristina
Passone, Maria Alejandra
author Sosa, Ana Laura
author_facet Sosa, Ana Laura
Girardi, Natalia Soledad
Rosso, Laura Cristina
Passone, Maria Alejandra
author_role author
author2 Girardi, Natalia Soledad
Rosso, Laura Cristina
Passone, Maria Alejandra
author2_role author
author
author
dc.subject.none.fl_str_mv BIOCONTROL
NACOBBUS ABERRANS
TOMATO
PURPUREOCILLUM LILACINUM
BOCCOLI EXTRACTS
topic BIOCONTROL
NACOBBUS ABERRANS
TOMATO
PURPUREOCILLUM LILACINUM
BOCCOLI EXTRACTS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Microorganisms in the tomato rhizosphere ecosystem develop associations, many of which canbe beneficial or pathogenic for this crop. The plant parasitic nematode (PPN) Nacobbus sp. cause significant economic losses to horticultural farmers in Argentina. The distribution of this genusis limited to the American continent and is consider a quarantine pest. Biological control strategies of PPN are environmentally friendly alternatives that ensure the sustainability of the horticultural system. The aim of this study was to evaluate the potential of the combined application of broccoli aqueous extract (BAE) and Purpureocillium lilacinum SR14 fungus in tomato plants (Solanumlycopersicum var. Platense) for the management of N. aberrans s.l. Three-week-old seedlings were transplanted into pots containing the following treatments: T1: control (sterile horticultural soil), T2: naturally infested horticultural soil (NIHS), T3: NIHS + SR14(1x10exp6 spores/g), T4:NIHS + BAE(12%), T5: SHNI + SR14(1x10exp6 spores/g) + BAE(12%). Six replicates per treatment were carried out and plants were maintained in a green house. Data, collected at 120 days post transplant,revealed that tomato growth was affected in NIHS(T2). Plants showed a 47% and 30% reduction in weight and length, respectively, compared to uninfected soil (T1). The highest number (4 tomatoes/plant) and weight mean (80 g) of fruits were recorded in T4. The fungus alone or combined with BAE (T3, T4 and T5) effectively controlled N. aberrans s.l. population (reduction percentages: galls = 35, 90 and 93%, egg masses = 5,94 and 90% and reproduction factor = 90, 93 and 98%). Physicochemical and biological analyzes were also performed to evaluate the effect of the treatment on the soil ecosystem. The data showed an increase in organic matter for all treatments compared to the initial condition. Nutrient values such as P, N and Swere maintained throughout the trial. Regarding the soil mycobiota, the fungal count (cfu/g) was around 106 for all treatments, but a lower diversity was observed in T5 with a prevalence of P. lilacinum (3x10exp4). As regards the nematofauna, treatments did not significantly influence the number of individuals (n°/g of soil) belonging to the different trophic groups (phytopathogens:16, bacteriophages: 19, predators: 10 and fungivores: 15), with the exception of T4, where ahigher count of omnivores was observed (30). Finally, studies on microbial activity revealed that treatments did not influence the counts (MPN/g) of ammonifying (mean: 1.9x10exp2) and denitrifying agents (mean: 2.7x1013), while T4 and T5 reduced the density of 1 log unit of nitrate- reducing bacteria (mean: 3.4x10exp6) and aerobic-heterotrophic bacteria (mean: 3.9x10exp8). In conclusion, the integrated strategy reveal a synergistic effect for the control of N. aberrans s.l.,without affecting tomato plant development, nutrient content and soil microbiome.
Fil: Sosa, Ana Laura. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Ecología Microbiana; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina
Fil: Girardi, Natalia Soledad. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Ecología Microbiana; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rosso, Laura Cristina. Istituto Per La Protezione Sostenibile Delle Piante; Italia
Fil: Passone, Maria Alejandra. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigación en Micología y Micotoxicología. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación en Micología y Micotoxicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
35th Symposium of the European Society of Nematologists
Cordoba
España
European Society of Nematologists
Consejo Superior de Investigaciones Científicas
Instituto Andaluz de Investigación y Formación Agraria, Pesquera, Alimentaria y de la Producción Ecológica
description Microorganisms in the tomato rhizosphere ecosystem develop associations, many of which canbe beneficial or pathogenic for this crop. The plant parasitic nematode (PPN) Nacobbus sp. cause significant economic losses to horticultural farmers in Argentina. The distribution of this genusis limited to the American continent and is consider a quarantine pest. Biological control strategies of PPN are environmentally friendly alternatives that ensure the sustainability of the horticultural system. The aim of this study was to evaluate the potential of the combined application of broccoli aqueous extract (BAE) and Purpureocillium lilacinum SR14 fungus in tomato plants (Solanumlycopersicum var. Platense) for the management of N. aberrans s.l. Three-week-old seedlings were transplanted into pots containing the following treatments: T1: control (sterile horticultural soil), T2: naturally infested horticultural soil (NIHS), T3: NIHS + SR14(1x10exp6 spores/g), T4:NIHS + BAE(12%), T5: SHNI + SR14(1x10exp6 spores/g) + BAE(12%). Six replicates per treatment were carried out and plants were maintained in a green house. Data, collected at 120 days post transplant,revealed that tomato growth was affected in NIHS(T2). Plants showed a 47% and 30% reduction in weight and length, respectively, compared to uninfected soil (T1). The highest number (4 tomatoes/plant) and weight mean (80 g) of fruits were recorded in T4. The fungus alone or combined with BAE (T3, T4 and T5) effectively controlled N. aberrans s.l. population (reduction percentages: galls = 35, 90 and 93%, egg masses = 5,94 and 90% and reproduction factor = 90, 93 and 98%). Physicochemical and biological analyzes were also performed to evaluate the effect of the treatment on the soil ecosystem. The data showed an increase in organic matter for all treatments compared to the initial condition. Nutrient values such as P, N and Swere maintained throughout the trial. Regarding the soil mycobiota, the fungal count (cfu/g) was around 106 for all treatments, but a lower diversity was observed in T5 with a prevalence of P. lilacinum (3x10exp4). As regards the nematofauna, treatments did not significantly influence the number of individuals (n°/g of soil) belonging to the different trophic groups (phytopathogens:16, bacteriophages: 19, predators: 10 and fungivores: 15), with the exception of T4, where ahigher count of omnivores was observed (30). Finally, studies on microbial activity revealed that treatments did not influence the counts (MPN/g) of ammonifying (mean: 1.9x10exp2) and denitrifying agents (mean: 2.7x1013), while T4 and T5 reduced the density of 1 log unit of nitrate- reducing bacteria (mean: 3.4x10exp6) and aerobic-heterotrophic bacteria (mean: 3.9x10exp8). In conclusion, the integrated strategy reveal a synergistic effect for the control of N. aberrans s.l.,without affecting tomato plant development, nutrient content and soil microbiome.
publishDate 2024
dc.date.none.fl_str_mv 2024
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info:eu-repo/semantics/conferenceObject
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info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
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dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/238304
Integrated strategies for the control of Nacobbus aberrans s.l. in tomato; 35th Symposium of the European Society of Nematologists; Cordoba; España; 2024; 290-291
CONICET Digital
CONICET
url http://hdl.handle.net/11336/238304
identifier_str_mv Integrated strategies for the control of Nacobbus aberrans s.l. in tomato; 35th Symposium of the European Society of Nematologists; Cordoba; España; 2024; 290-291
CONICET Digital
CONICET
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language eng
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