Comparative plasma pharmacokinetics, tissue distribution and efficacy of two formulations ciprofloxacin-based in mouse

Autores
Breda, Susana Andrea; González, Carlos; Confalonieri, Alejandra; Sanchez Bruni, Sergio Fabian; Manzo, Ruben Hilario; Olivera, María Eugenia
Año de publicación
2009
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The aim of this study was to assess the plasma pharmacokinetics, tissue distribution and efficacy of a formulation ciprofloxacin (CIP)-Aluminum complex based (CIP-Al) in comparison with a conventional CIP formulation. For this study 96 Balb-C mice were divided in two groups and treated as follows: Group I received orally a single dose of CIP 5 mg/kg. Animals of Group II were identically treated but with CIP-AL formulation. Samples of blood, lung, intestine and kidney, were taken over 12 h post-treatment, and frozen until analysis by HPLC. The experimental efficacy study was based in an experimental Salmonella infection model in mice. Mice were divided into 3 groups: Control (distilled water treatment), CIP and CIP-Al. After 5 days of treatment survival was recorded. The plasma pharmacokinetics study outcomes revealed similar AUC values for CIP and CIP-Al. However, CIP concentration levels were statistically (P< 0.05) higher in lung, intestine and kidney after CIP-Al administration than those obtained for CIP conventional formulation group after 1h post treatment. The higher tissue concentrations of CIP-Al may have contributed to the observed efficacy trend where survived 33% of mice treated compared with the conventional CIP formulation assayed (0%). This fact would be related to the improved aqueous compatibility of CIP after aluminum complexation. We found that CIP-Al is at least as effective as CIP and exhibited advantageous pharmacokinetic and dispositional properties. It may become a valuable asset based on its formulation versatility due to higher solubility.
Fil: Breda, Susana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina
Fil: González, Carlos. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina
Fil: Confalonieri, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina
Fil: Sanchez Bruni, Sergio Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina
Fil: Manzo, Ruben Hilario. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina
Fil: Olivera, María Eugenia. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina
LXI Reunion Anual de Sociedad Argentina de Farmacología Experimental
Rosario
Argentina
Sociedad Argentina de Farmacología Experimental
Materia
CIPROFLOXACIN-ALUMINIUM
PHARMACOKINETICS
MICE
TISSUE DISTRIBUTION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/242057
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/242057
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Comparative plasma pharmacokinetics, tissue distribution and efficacy of two formulations ciprofloxacin-based in mouseBreda, Susana AndreaGonzález, CarlosConfalonieri, AlejandraSanchez Bruni, Sergio FabianManzo, Ruben HilarioOlivera, María EugeniaCIPROFLOXACIN-ALUMINIUMPHARMACOKINETICSMICETISSUE DISTRIBUTIONhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3The aim of this study was to assess the plasma pharmacokinetics, tissue distribution and efficacy of a formulation ciprofloxacin (CIP)-Aluminum complex based (CIP-Al) in comparison with a conventional CIP formulation. For this study 96 Balb-C mice were divided in two groups and treated as follows: Group I received orally a single dose of CIP 5 mg/kg. Animals of Group II were identically treated but with CIP-AL formulation. Samples of blood, lung, intestine and kidney, were taken over 12 h post-treatment, and frozen until analysis by HPLC. The experimental efficacy study was based in an experimental Salmonella infection model in mice. Mice were divided into 3 groups: Control (distilled water treatment), CIP and CIP-Al. After 5 days of treatment survival was recorded. The plasma pharmacokinetics study outcomes revealed similar AUC values for CIP and CIP-Al. However, CIP concentration levels were statistically (P< 0.05) higher in lung, intestine and kidney after CIP-Al administration than those obtained for CIP conventional formulation group after 1h post treatment. The higher tissue concentrations of CIP-Al may have contributed to the observed efficacy trend where survived 33% of mice treated compared with the conventional CIP formulation assayed (0%). This fact would be related to the improved aqueous compatibility of CIP after aluminum complexation. We found that CIP-Al is at least as effective as CIP and exhibited advantageous pharmacokinetic and dispositional properties. It may become a valuable asset based on its formulation versatility due to higher solubility.Fil: Breda, Susana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; ArgentinaFil: González, Carlos. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; ArgentinaFil: Confalonieri, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; ArgentinaFil: Sanchez Bruni, Sergio Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; ArgentinaFil: Manzo, Ruben Hilario. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; ArgentinaFil: Olivera, María Eugenia. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; ArgentinaLXI Reunion Anual de Sociedad Argentina de Farmacología ExperimentalRosarioArgentinaSociedad Argentina de Farmacología ExperimentalSociedad Argentina de Farmacología Experimental2009info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/mswordapplication/pdfhttp://hdl.handle.net/11336/242057Comparative plasma pharmacokinetics, tissue distribution and efficacy of two formulations ciprofloxacin-based in mouse; LXI Reunion Anual de Sociedad Argentina de Farmacología Experimental; Rosario; Argentina; 2009; 28-28CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://aafeargentina.org/wp-content/uploads/2021/07/ACTA_2009.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:02:13Zoai:ri.conicet.gov.ar:11336/242057instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:02:13.845CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Comparative plasma pharmacokinetics, tissue distribution and efficacy of two formulations ciprofloxacin-based in mouse
title Comparative plasma pharmacokinetics, tissue distribution and efficacy of two formulations ciprofloxacin-based in mouse
spellingShingle Comparative plasma pharmacokinetics, tissue distribution and efficacy of two formulations ciprofloxacin-based in mouse
Breda, Susana Andrea
CIPROFLOXACIN-ALUMINIUM
PHARMACOKINETICS
MICE
TISSUE DISTRIBUTION
title_short Comparative plasma pharmacokinetics, tissue distribution and efficacy of two formulations ciprofloxacin-based in mouse
title_full Comparative plasma pharmacokinetics, tissue distribution and efficacy of two formulations ciprofloxacin-based in mouse
title_fullStr Comparative plasma pharmacokinetics, tissue distribution and efficacy of two formulations ciprofloxacin-based in mouse
title_full_unstemmed Comparative plasma pharmacokinetics, tissue distribution and efficacy of two formulations ciprofloxacin-based in mouse
title_sort Comparative plasma pharmacokinetics, tissue distribution and efficacy of two formulations ciprofloxacin-based in mouse
dc.creator.none.fl_str_mv Breda, Susana Andrea
González, Carlos
Confalonieri, Alejandra
Sanchez Bruni, Sergio Fabian
Manzo, Ruben Hilario
Olivera, María Eugenia
author Breda, Susana Andrea
author_facet Breda, Susana Andrea
González, Carlos
Confalonieri, Alejandra
Sanchez Bruni, Sergio Fabian
Manzo, Ruben Hilario
Olivera, María Eugenia
author_role author
author2 González, Carlos
Confalonieri, Alejandra
Sanchez Bruni, Sergio Fabian
Manzo, Ruben Hilario
Olivera, María Eugenia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv CIPROFLOXACIN-ALUMINIUM
PHARMACOKINETICS
MICE
TISSUE DISTRIBUTION
topic CIPROFLOXACIN-ALUMINIUM
PHARMACOKINETICS
MICE
TISSUE DISTRIBUTION
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The aim of this study was to assess the plasma pharmacokinetics, tissue distribution and efficacy of a formulation ciprofloxacin (CIP)-Aluminum complex based (CIP-Al) in comparison with a conventional CIP formulation. For this study 96 Balb-C mice were divided in two groups and treated as follows: Group I received orally a single dose of CIP 5 mg/kg. Animals of Group II were identically treated but with CIP-AL formulation. Samples of blood, lung, intestine and kidney, were taken over 12 h post-treatment, and frozen until analysis by HPLC. The experimental efficacy study was based in an experimental Salmonella infection model in mice. Mice were divided into 3 groups: Control (distilled water treatment), CIP and CIP-Al. After 5 days of treatment survival was recorded. The plasma pharmacokinetics study outcomes revealed similar AUC values for CIP and CIP-Al. However, CIP concentration levels were statistically (P< 0.05) higher in lung, intestine and kidney after CIP-Al administration than those obtained for CIP conventional formulation group after 1h post treatment. The higher tissue concentrations of CIP-Al may have contributed to the observed efficacy trend where survived 33% of mice treated compared with the conventional CIP formulation assayed (0%). This fact would be related to the improved aqueous compatibility of CIP after aluminum complexation. We found that CIP-Al is at least as effective as CIP and exhibited advantageous pharmacokinetic and dispositional properties. It may become a valuable asset based on its formulation versatility due to higher solubility.
Fil: Breda, Susana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina
Fil: González, Carlos. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina
Fil: Confalonieri, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina
Fil: Sanchez Bruni, Sergio Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina
Fil: Manzo, Ruben Hilario. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina
Fil: Olivera, María Eugenia. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina
LXI Reunion Anual de Sociedad Argentina de Farmacología Experimental
Rosario
Argentina
Sociedad Argentina de Farmacología Experimental
description The aim of this study was to assess the plasma pharmacokinetics, tissue distribution and efficacy of a formulation ciprofloxacin (CIP)-Aluminum complex based (CIP-Al) in comparison with a conventional CIP formulation. For this study 96 Balb-C mice were divided in two groups and treated as follows: Group I received orally a single dose of CIP 5 mg/kg. Animals of Group II were identically treated but with CIP-AL formulation. Samples of blood, lung, intestine and kidney, were taken over 12 h post-treatment, and frozen until analysis by HPLC. The experimental efficacy study was based in an experimental Salmonella infection model in mice. Mice were divided into 3 groups: Control (distilled water treatment), CIP and CIP-Al. After 5 days of treatment survival was recorded. The plasma pharmacokinetics study outcomes revealed similar AUC values for CIP and CIP-Al. However, CIP concentration levels were statistically (P< 0.05) higher in lung, intestine and kidney after CIP-Al administration than those obtained for CIP conventional formulation group after 1h post treatment. The higher tissue concentrations of CIP-Al may have contributed to the observed efficacy trend where survived 33% of mice treated compared with the conventional CIP formulation assayed (0%). This fact would be related to the improved aqueous compatibility of CIP after aluminum complexation. We found that CIP-Al is at least as effective as CIP and exhibited advantageous pharmacokinetic and dispositional properties. It may become a valuable asset based on its formulation versatility due to higher solubility.
publishDate 2009
dc.date.none.fl_str_mv 2009
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/242057
Comparative plasma pharmacokinetics, tissue distribution and efficacy of two formulations ciprofloxacin-based in mouse; LXI Reunion Anual de Sociedad Argentina de Farmacología Experimental; Rosario; Argentina; 2009; 28-28
CONICET Digital
CONICET
url http://hdl.handle.net/11336/242057
identifier_str_mv Comparative plasma pharmacokinetics, tissue distribution and efficacy of two formulations ciprofloxacin-based in mouse; LXI Reunion Anual de Sociedad Argentina de Farmacología Experimental; Rosario; Argentina; 2009; 28-28
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://aafeargentina.org/wp-content/uploads/2021/07/ACTA_2009.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/msword
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Farmacología Experimental
publisher.none.fl_str_mv Sociedad Argentina de Farmacología Experimental
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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