Adsorption of the bacteriocins produced by Lactobacillus curvatus CRL705 on a multilayer-LLDPE film for food-packaging applications

Autores
Blanco Massani, Mariana Raquel; Vignolo, Graciela Margarita; Eisenberg, Patricia; Morando, Pedro Juan
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Adsorption of bacteriocins produced by Lactobacillus curvatus CRL705, lactocin 705 (whose activity depends upon complementation of two peptides, lac705α and lac705β) and bacteriocin/s with strong anti-Listeria activity, on a multilayer film was investigated. Lactocin 705 adsorption equilibrium at 30 °C was reached from 1 h of film contact. This bacteriocin exhibited a Langmuir-type adsorption, showing a mass adsorption maximum of 0.72 ± 0.05 μg cm−2 and a minimum inhibition concentration of 1 μg ml−1. The influence of impurities, generated from bacteriocinogenic strains growth, on bacteriocins adsorption to the film was investigated by inhibition area evaluation in semisolid agar. Impurities from LAB growth strongly influenced adsorption and lactocin 705 antimicrobial activity on the film, while antilisterial bacteriocin/s adsorption remained unaffected. To explain these results, a lack of lac705β and lac705α peptides complementation necessary for antimicrobial activity, while no interactions among impurities and antilisterial bacteriocin/s during adsorption was suggested. Antilisterial bacteriocin/s activity on the film was not influenced by lactocin 705 adsorption; conformational reorganization of adsorbed antilisterial bacteriocin/s in the presence of lactocin 705 could allow the adsorption of both bacteriocins while maintaining antilisterial antimicrobial activity. This study highlights the technological importance of adsorption optimization to obtain effective antimicrobial food packaging systems.
Fil: Blanco Massani, Mariana Raquel. Instituto Nacional de Tecnología Industrial; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vignolo, Graciela Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Eisenberg, Patricia. Instituto Nacional de Tecnología Industrial; Argentina. Universidad Nacional de San Martín; Argentina
Fil: Morando, Pedro Juan. Comisión Nacional de Energía Atómica. Centro Atómico Constituyentes; Argentina. Universidad Nacional de San Martín. Instituto Sabato; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Bacteriocins
Adsorption
Antilisteria
Active Packaging
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/28115
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/28115
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Adsorption of the bacteriocins produced by Lactobacillus curvatus CRL705 on a multilayer-LLDPE film for food-packaging applicationsBlanco Massani, Mariana RaquelVignolo, Graciela MargaritaEisenberg, PatriciaMorando, Pedro JuanBacteriocinsAdsorptionAntilisteriaActive Packaginghttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Adsorption of bacteriocins produced by Lactobacillus curvatus CRL705, lactocin 705 (whose activity depends upon complementation of two peptides, lac705α and lac705β) and bacteriocin/s with strong anti-Listeria activity, on a multilayer film was investigated. Lactocin 705 adsorption equilibrium at 30 °C was reached from 1 h of film contact. This bacteriocin exhibited a Langmuir-type adsorption, showing a mass adsorption maximum of 0.72 ± 0.05 μg cm−2 and a minimum inhibition concentration of 1 μg ml−1. The influence of impurities, generated from bacteriocinogenic strains growth, on bacteriocins adsorption to the film was investigated by inhibition area evaluation in semisolid agar. Impurities from LAB growth strongly influenced adsorption and lactocin 705 antimicrobial activity on the film, while antilisterial bacteriocin/s adsorption remained unaffected. To explain these results, a lack of lac705β and lac705α peptides complementation necessary for antimicrobial activity, while no interactions among impurities and antilisterial bacteriocin/s during adsorption was suggested. Antilisterial bacteriocin/s activity on the film was not influenced by lactocin 705 adsorption; conformational reorganization of adsorbed antilisterial bacteriocin/s in the presence of lactocin 705 could allow the adsorption of both bacteriocins while maintaining antilisterial antimicrobial activity. This study highlights the technological importance of adsorption optimization to obtain effective antimicrobial food packaging systems.Fil: Blanco Massani, Mariana Raquel. Instituto Nacional de Tecnología Industrial; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Vignolo, Graciela Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Eisenberg, Patricia. Instituto Nacional de Tecnología Industrial; Argentina. Universidad Nacional de San Martín; ArgentinaFil: Morando, Pedro Juan. Comisión Nacional de Energía Atómica. Centro Atómico Constituyentes; Argentina. Universidad Nacional de San Martín. Instituto Sabato; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2013-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/28115Blanco Massani, Mariana Raquel; Vignolo, Graciela Margarita; Eisenberg, Patricia; Morando, Pedro Juan; Adsorption of the bacteriocins produced by Lactobacillus curvatus CRL705 on a multilayer-LLDPE film for food-packaging applications; Elsevier; LWT - Food Science and Technology; 53; 1; 1-2013; 128-1381096-1127CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.lwt.2013.01.018info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0023643813000327info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:30:16Zoai:ri.conicet.gov.ar:11336/28115instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:30:16.729CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Adsorption of the bacteriocins produced by Lactobacillus curvatus CRL705 on a multilayer-LLDPE film for food-packaging applications
title Adsorption of the bacteriocins produced by Lactobacillus curvatus CRL705 on a multilayer-LLDPE film for food-packaging applications
spellingShingle Adsorption of the bacteriocins produced by Lactobacillus curvatus CRL705 on a multilayer-LLDPE film for food-packaging applications
Blanco Massani, Mariana Raquel
Bacteriocins
Adsorption
Antilisteria
Active Packaging
title_short Adsorption of the bacteriocins produced by Lactobacillus curvatus CRL705 on a multilayer-LLDPE film for food-packaging applications
title_full Adsorption of the bacteriocins produced by Lactobacillus curvatus CRL705 on a multilayer-LLDPE film for food-packaging applications
title_fullStr Adsorption of the bacteriocins produced by Lactobacillus curvatus CRL705 on a multilayer-LLDPE film for food-packaging applications
title_full_unstemmed Adsorption of the bacteriocins produced by Lactobacillus curvatus CRL705 on a multilayer-LLDPE film for food-packaging applications
title_sort Adsorption of the bacteriocins produced by Lactobacillus curvatus CRL705 on a multilayer-LLDPE film for food-packaging applications
dc.creator.none.fl_str_mv Blanco Massani, Mariana Raquel
Vignolo, Graciela Margarita
Eisenberg, Patricia
Morando, Pedro Juan
author Blanco Massani, Mariana Raquel
author_facet Blanco Massani, Mariana Raquel
Vignolo, Graciela Margarita
Eisenberg, Patricia
Morando, Pedro Juan
author_role author
author2 Vignolo, Graciela Margarita
Eisenberg, Patricia
Morando, Pedro Juan
author2_role author
author
author
dc.subject.none.fl_str_mv Bacteriocins
Adsorption
Antilisteria
Active Packaging
topic Bacteriocins
Adsorption
Antilisteria
Active Packaging
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Adsorption of bacteriocins produced by Lactobacillus curvatus CRL705, lactocin 705 (whose activity depends upon complementation of two peptides, lac705α and lac705β) and bacteriocin/s with strong anti-Listeria activity, on a multilayer film was investigated. Lactocin 705 adsorption equilibrium at 30 °C was reached from 1 h of film contact. This bacteriocin exhibited a Langmuir-type adsorption, showing a mass adsorption maximum of 0.72 ± 0.05 μg cm−2 and a minimum inhibition concentration of 1 μg ml−1. The influence of impurities, generated from bacteriocinogenic strains growth, on bacteriocins adsorption to the film was investigated by inhibition area evaluation in semisolid agar. Impurities from LAB growth strongly influenced adsorption and lactocin 705 antimicrobial activity on the film, while antilisterial bacteriocin/s adsorption remained unaffected. To explain these results, a lack of lac705β and lac705α peptides complementation necessary for antimicrobial activity, while no interactions among impurities and antilisterial bacteriocin/s during adsorption was suggested. Antilisterial bacteriocin/s activity on the film was not influenced by lactocin 705 adsorption; conformational reorganization of adsorbed antilisterial bacteriocin/s in the presence of lactocin 705 could allow the adsorption of both bacteriocins while maintaining antilisterial antimicrobial activity. This study highlights the technological importance of adsorption optimization to obtain effective antimicrobial food packaging systems.
Fil: Blanco Massani, Mariana Raquel. Instituto Nacional de Tecnología Industrial; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vignolo, Graciela Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Eisenberg, Patricia. Instituto Nacional de Tecnología Industrial; Argentina. Universidad Nacional de San Martín; Argentina
Fil: Morando, Pedro Juan. Comisión Nacional de Energía Atómica. Centro Atómico Constituyentes; Argentina. Universidad Nacional de San Martín. Instituto Sabato; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Adsorption of bacteriocins produced by Lactobacillus curvatus CRL705, lactocin 705 (whose activity depends upon complementation of two peptides, lac705α and lac705β) and bacteriocin/s with strong anti-Listeria activity, on a multilayer film was investigated. Lactocin 705 adsorption equilibrium at 30 °C was reached from 1 h of film contact. This bacteriocin exhibited a Langmuir-type adsorption, showing a mass adsorption maximum of 0.72 ± 0.05 μg cm−2 and a minimum inhibition concentration of 1 μg ml−1. The influence of impurities, generated from bacteriocinogenic strains growth, on bacteriocins adsorption to the film was investigated by inhibition area evaluation in semisolid agar. Impurities from LAB growth strongly influenced adsorption and lactocin 705 antimicrobial activity on the film, while antilisterial bacteriocin/s adsorption remained unaffected. To explain these results, a lack of lac705β and lac705α peptides complementation necessary for antimicrobial activity, while no interactions among impurities and antilisterial bacteriocin/s during adsorption was suggested. Antilisterial bacteriocin/s activity on the film was not influenced by lactocin 705 adsorption; conformational reorganization of adsorbed antilisterial bacteriocin/s in the presence of lactocin 705 could allow the adsorption of both bacteriocins while maintaining antilisterial antimicrobial activity. This study highlights the technological importance of adsorption optimization to obtain effective antimicrobial food packaging systems.
publishDate 2013
dc.date.none.fl_str_mv 2013-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/28115
Blanco Massani, Mariana Raquel; Vignolo, Graciela Margarita; Eisenberg, Patricia; Morando, Pedro Juan; Adsorption of the bacteriocins produced by Lactobacillus curvatus CRL705 on a multilayer-LLDPE film for food-packaging applications; Elsevier; LWT - Food Science and Technology; 53; 1; 1-2013; 128-138
1096-1127
CONICET Digital
CONICET
url http://hdl.handle.net/11336/28115
identifier_str_mv Blanco Massani, Mariana Raquel; Vignolo, Graciela Margarita; Eisenberg, Patricia; Morando, Pedro Juan; Adsorption of the bacteriocins produced by Lactobacillus curvatus CRL705 on a multilayer-LLDPE film for food-packaging applications; Elsevier; LWT - Food Science and Technology; 53; 1; 1-2013; 128-138
1096-1127
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.lwt.2013.01.018
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0023643813000327
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846082778612891648
score 13.22299

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