Comparative proteomics of soluble factors secreted by human breast adipose tissue from tumor and normal breast
- Autores
- Fletcher, Sabrina Johanna; Hapon, María Belén; Callegari, E.; Crosbie, M. L.; Santiso, N.; Ursino, A.; Amato, A. R.; Gutiérrez, A.; Sacca, Paula Alejandra; Dreszman, R.; Pérez, A.; Caron, Ruben Walter; Calvo, Juan Carlos; Pistone Creydt, Virginia
- Año de publicación
- 2018
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Tumor progression depends on the tumor-stroma interaction. In the breast, adipose tissue is the predominant stromal type. We have previously demonstrated that conditioned media (CMs) from explants of human adipose tissue of tumor breasts (hATT) increase proliferation and migration of breast cancer epithelial cells when compared to human adipose tissue from normal breasts (hATN). In this work, we aim to identify specific proteins and molecular/biological pathways associated with the secretion profile of hATT and hATN explants. hATT-CMs and hATN-CMs were separated by SDS-PAGE and analyzed by means of two-dimensional nano-liquid chromatography-mass spectrometry. The data was analyzed using ProteoIQ and FunRich software. In addition, 42 cytokines from hATTCMs and hATN-CMs were assayed by a protein antibody assay. Compared to hATNCMs, hATT-CMs showed greater protein diversity. We found that hATT-CMs presented a greater amount of proteins related to complement system activity, metabolism and immune system, as well as proteins involved in a variety of biological processes such as signal transduction and cell communication. Specifically, apolipoprotein AI and AII, complement component 3, and vimentin and desmin were significantly increased in hATT-CMs versus hATN-CMs. Moreover, a multivariate discriminant analysis of the cytokines detected by the array showed that IL-6, MCP-2 and GRO cytokines were sufficient and necessary to differentiate hATT-CMs from hATN-CMs. This analysis also showed that the levels of these three cytokines, taken together, correlated with stage and histological grade of the tumor in the hATT-CMs group, and with body mass index in the hATN-CMs group.
Fil: Fletcher, Sabrina Johanna. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Hapon, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Callegari, E.. University Of South Dakota; Estados Unidos
Fil: Crosbie, M. L.. Complejo Médico Policial "Churruca Visca"; Argentina
Fil: Santiso, N.. Complejo Médico Policial "Churruca Visca"; Argentina
Fil: Ursino, A.. Complejo Médico Policial "Churruca Visca"; Argentina
Fil: Amato, A. R.. Complejo Médico Policial "Churruca Visca"; Argentina
Fil: Gutiérrez, A.. Complejo Médico Policial "Churruca Visca"; Argentina
Fil: Sacca, Paula Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Dreszman, R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Pérez, A.. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Matemática; Argentina
Fil: Caron, Ruben Walter. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Calvo, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Pistone Creydt, Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina - Materia
-
ADIPOSE TISSUE
EPITHELIAL-STROMAL INTERACTION
HUMAN BREAST CANCER
PROTEOMICS ANALYSIS
TUMOR MICROENVIRONMENT - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/92113
Ver los metadatos del registro completo
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Comparative proteomics of soluble factors secreted by human breast adipose tissue from tumor and normal breastFletcher, Sabrina JohannaHapon, María BelénCallegari, E.Crosbie, M. L.Santiso, N.Ursino, A.Amato, A. R.Gutiérrez, A.Sacca, Paula AlejandraDreszman, R.Pérez, A.Caron, Ruben WalterCalvo, Juan CarlosPistone Creydt, VirginiaADIPOSE TISSUEEPITHELIAL-STROMAL INTERACTIONHUMAN BREAST CANCERPROTEOMICS ANALYSISTUMOR MICROENVIRONMENThttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Tumor progression depends on the tumor-stroma interaction. In the breast, adipose tissue is the predominant stromal type. We have previously demonstrated that conditioned media (CMs) from explants of human adipose tissue of tumor breasts (hATT) increase proliferation and migration of breast cancer epithelial cells when compared to human adipose tissue from normal breasts (hATN). In this work, we aim to identify specific proteins and molecular/biological pathways associated with the secretion profile of hATT and hATN explants. hATT-CMs and hATN-CMs were separated by SDS-PAGE and analyzed by means of two-dimensional nano-liquid chromatography-mass spectrometry. The data was analyzed using ProteoIQ and FunRich software. In addition, 42 cytokines from hATTCMs and hATN-CMs were assayed by a protein antibody assay. Compared to hATNCMs, hATT-CMs showed greater protein diversity. We found that hATT-CMs presented a greater amount of proteins related to complement system activity, metabolism and immune system, as well as proteins involved in a variety of biological processes such as signal transduction and cell communication. Specifically, apolipoprotein AI and AII, complement component 3, and vimentin and desmin were significantly increased in hATT-CMs versus hATN-CMs. Moreover, a multivariate discriminant analysis of the cytokines detected by the array showed that IL-6, MCP-2 and GRO cytokines were sufficient and necessary to differentiate hATT-CMs from hATN-CMs. This analysis also showed that the levels of these three cytokines, taken together, correlated with stage and histological grade of the tumor in the hATT-CMs group, and with body mass index in the hATN-CMs group.Fil: Fletcher, Sabrina Johanna. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Hapon, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Callegari, E.. University Of South Dakota; Estados UnidosFil: Crosbie, M. L.. Complejo Médico Policial "Churruca Visca"; ArgentinaFil: Santiso, N.. Complejo Médico Policial "Churruca Visca"; ArgentinaFil: Ursino, A.. Complejo Médico Policial "Churruca Visca"; ArgentinaFil: Amato, A. R.. Complejo Médico Policial "Churruca Visca"; ArgentinaFil: Gutiérrez, A.. Complejo Médico Policial "Churruca Visca"; ArgentinaFil: Sacca, Paula Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Dreszman, R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Pérez, A.. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Matemática; ArgentinaFil: Caron, Ruben Walter. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Calvo, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Pistone Creydt, Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaImpact Journals LLC2018-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/92113Fletcher, Sabrina Johanna; Hapon, María Belén; Callegari, E.; Crosbie, M. L.; Santiso, N.; et al.; Comparative proteomics of soluble factors secreted by human breast adipose tissue from tumor and normal breast; Impact Journals LLC; Oncotarget; 9; 57; 7-2018; 31007-310171949-2553CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.18632/oncotarget.25749info:eu-repo/semantics/altIdentifier/url/http://www.oncotarget.com/index.php?journal=oncotarget&page=article&op=view&path[]=25749&path[]=80555info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:02:23Zoai:ri.conicet.gov.ar:11336/92113instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:02:24.157CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Comparative proteomics of soluble factors secreted by human breast adipose tissue from tumor and normal breast |
| title |
Comparative proteomics of soluble factors secreted by human breast adipose tissue from tumor and normal breast |
| spellingShingle |
Comparative proteomics of soluble factors secreted by human breast adipose tissue from tumor and normal breast Fletcher, Sabrina Johanna ADIPOSE TISSUE EPITHELIAL-STROMAL INTERACTION HUMAN BREAST CANCER PROTEOMICS ANALYSIS TUMOR MICROENVIRONMENT |
| title_short |
Comparative proteomics of soluble factors secreted by human breast adipose tissue from tumor and normal breast |
| title_full |
Comparative proteomics of soluble factors secreted by human breast adipose tissue from tumor and normal breast |
| title_fullStr |
Comparative proteomics of soluble factors secreted by human breast adipose tissue from tumor and normal breast |
| title_full_unstemmed |
Comparative proteomics of soluble factors secreted by human breast adipose tissue from tumor and normal breast |
| title_sort |
Comparative proteomics of soluble factors secreted by human breast adipose tissue from tumor and normal breast |
| dc.creator.none.fl_str_mv |
Fletcher, Sabrina Johanna Hapon, María Belén Callegari, E. Crosbie, M. L. Santiso, N. Ursino, A. Amato, A. R. Gutiérrez, A. Sacca, Paula Alejandra Dreszman, R. Pérez, A. Caron, Ruben Walter Calvo, Juan Carlos Pistone Creydt, Virginia |
| author |
Fletcher, Sabrina Johanna |
| author_facet |
Fletcher, Sabrina Johanna Hapon, María Belén Callegari, E. Crosbie, M. L. Santiso, N. Ursino, A. Amato, A. R. Gutiérrez, A. Sacca, Paula Alejandra Dreszman, R. Pérez, A. Caron, Ruben Walter Calvo, Juan Carlos Pistone Creydt, Virginia |
| author_role |
author |
| author2 |
Hapon, María Belén Callegari, E. Crosbie, M. L. Santiso, N. Ursino, A. Amato, A. R. Gutiérrez, A. Sacca, Paula Alejandra Dreszman, R. Pérez, A. Caron, Ruben Walter Calvo, Juan Carlos Pistone Creydt, Virginia |
| author2_role |
author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
ADIPOSE TISSUE EPITHELIAL-STROMAL INTERACTION HUMAN BREAST CANCER PROTEOMICS ANALYSIS TUMOR MICROENVIRONMENT |
| topic |
ADIPOSE TISSUE EPITHELIAL-STROMAL INTERACTION HUMAN BREAST CANCER PROTEOMICS ANALYSIS TUMOR MICROENVIRONMENT |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
| dc.description.none.fl_txt_mv |
Tumor progression depends on the tumor-stroma interaction. In the breast, adipose tissue is the predominant stromal type. We have previously demonstrated that conditioned media (CMs) from explants of human adipose tissue of tumor breasts (hATT) increase proliferation and migration of breast cancer epithelial cells when compared to human adipose tissue from normal breasts (hATN). In this work, we aim to identify specific proteins and molecular/biological pathways associated with the secretion profile of hATT and hATN explants. hATT-CMs and hATN-CMs were separated by SDS-PAGE and analyzed by means of two-dimensional nano-liquid chromatography-mass spectrometry. The data was analyzed using ProteoIQ and FunRich software. In addition, 42 cytokines from hATTCMs and hATN-CMs were assayed by a protein antibody assay. Compared to hATNCMs, hATT-CMs showed greater protein diversity. We found that hATT-CMs presented a greater amount of proteins related to complement system activity, metabolism and immune system, as well as proteins involved in a variety of biological processes such as signal transduction and cell communication. Specifically, apolipoprotein AI and AII, complement component 3, and vimentin and desmin were significantly increased in hATT-CMs versus hATN-CMs. Moreover, a multivariate discriminant analysis of the cytokines detected by the array showed that IL-6, MCP-2 and GRO cytokines were sufficient and necessary to differentiate hATT-CMs from hATN-CMs. This analysis also showed that the levels of these three cytokines, taken together, correlated with stage and histological grade of the tumor in the hATT-CMs group, and with body mass index in the hATN-CMs group. Fil: Fletcher, Sabrina Johanna. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina Fil: Hapon, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina Fil: Callegari, E.. University Of South Dakota; Estados Unidos Fil: Crosbie, M. L.. Complejo Médico Policial "Churruca Visca"; Argentina Fil: Santiso, N.. Complejo Médico Policial "Churruca Visca"; Argentina Fil: Ursino, A.. Complejo Médico Policial "Churruca Visca"; Argentina Fil: Amato, A. R.. Complejo Médico Policial "Churruca Visca"; Argentina Fil: Gutiérrez, A.. Complejo Médico Policial "Churruca Visca"; Argentina Fil: Sacca, Paula Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina Fil: Dreszman, R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina Fil: Pérez, A.. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Matemática; Argentina Fil: Caron, Ruben Walter. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina Fil: Calvo, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina Fil: Pistone Creydt, Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina |
| description |
Tumor progression depends on the tumor-stroma interaction. In the breast, adipose tissue is the predominant stromal type. We have previously demonstrated that conditioned media (CMs) from explants of human adipose tissue of tumor breasts (hATT) increase proliferation and migration of breast cancer epithelial cells when compared to human adipose tissue from normal breasts (hATN). In this work, we aim to identify specific proteins and molecular/biological pathways associated with the secretion profile of hATT and hATN explants. hATT-CMs and hATN-CMs were separated by SDS-PAGE and analyzed by means of two-dimensional nano-liquid chromatography-mass spectrometry. The data was analyzed using ProteoIQ and FunRich software. In addition, 42 cytokines from hATTCMs and hATN-CMs were assayed by a protein antibody assay. Compared to hATNCMs, hATT-CMs showed greater protein diversity. We found that hATT-CMs presented a greater amount of proteins related to complement system activity, metabolism and immune system, as well as proteins involved in a variety of biological processes such as signal transduction and cell communication. Specifically, apolipoprotein AI and AII, complement component 3, and vimentin and desmin were significantly increased in hATT-CMs versus hATN-CMs. Moreover, a multivariate discriminant analysis of the cytokines detected by the array showed that IL-6, MCP-2 and GRO cytokines were sufficient and necessary to differentiate hATT-CMs from hATN-CMs. This analysis also showed that the levels of these three cytokines, taken together, correlated with stage and histological grade of the tumor in the hATT-CMs group, and with body mass index in the hATN-CMs group. |
| publishDate |
2018 |
| dc.date.none.fl_str_mv |
2018-07 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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publishedVersion |
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http://hdl.handle.net/11336/92113 Fletcher, Sabrina Johanna; Hapon, María Belén; Callegari, E.; Crosbie, M. L.; Santiso, N.; et al.; Comparative proteomics of soluble factors secreted by human breast adipose tissue from tumor and normal breast; Impact Journals LLC; Oncotarget; 9; 57; 7-2018; 31007-31017 1949-2553 CONICET Digital CONICET |
| url |
http://hdl.handle.net/11336/92113 |
| identifier_str_mv |
Fletcher, Sabrina Johanna; Hapon, María Belén; Callegari, E.; Crosbie, M. L.; Santiso, N.; et al.; Comparative proteomics of soluble factors secreted by human breast adipose tissue from tumor and normal breast; Impact Journals LLC; Oncotarget; 9; 57; 7-2018; 31007-31017 1949-2553 CONICET Digital CONICET |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.18632/oncotarget.25749 info:eu-repo/semantics/altIdentifier/url/http://www.oncotarget.com/index.php?journal=oncotarget&page=article&op=view&path[]=25749&path[]=80555 |
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info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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openAccess |
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https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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application/pdf application/pdf application/pdf application/pdf |
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Impact Journals LLC |
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Impact Journals LLC |
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reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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