α-lipoic acid protects the ischemia reperfusion injury in human simultaneous kidney-pancreas transplantation.

Autores
Ambrosi, Nella Gabriela; Arrosagaray, Victoria; Guerrieri, Diego; Uva, Pablo Daniel; Petroni, Jorgelina; Buonpensiere, Mónica; Iovanna, Juan; León, Luis; Incardona, Claudio; Chuluyan, Hector Eduardo; Casadei, Domingo H.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Multiple factors have been implicated in the process of ischemia-reperfusion injury (IRI) in organ transplantation. Among these factors, oxidative damage seems to initiate the injury. α-lipoic acid (ALA) is a potent antioxidant that is used in patients with diabetic polyneuropathy. The aim of the present study was to determine the effect of ALA in patients undergoing simultaneous kidney-pancreas transplant by evaluating the functional recovery of the graft and biochemical markers of IRI. Methods: Twenty-six patients were included in the following groups: (i) untreated control; (ii) donor and recipient (DR) ALA-treated, in which ALA was administered both to the deceased donor and to the recipients; and (iii) recipient ALA-treated group. The expression of inflammatory genes, as observed in biopsies taken at the end of surgery, as well as the serum cytokines, secretory leukocyte protease inhibitor, regenerating islet-derived protein 3β/pancreatitis-associated protein, amylase, lipase, glucose, and creatinine levels were quantified as markers of organ function. Results: The DR group showed high levels of TGFβ and low levels of C3 and TNFα in the kidneys, whereas high levels of C3 and heme oxygenase were identified in pancreas biopsies. Decreases in serum IL-8, IL-6, secretory leukocyte protease inhibitor, and regenerating islet-derived protein 3 β/pancreatitis-associated protein were observed after surgery in the DR group. Serum lipase and amylase were lower in the DR group than in the control and recipient groups. Early kidney dysfunction and clinical pancreatitis were higher in the control group than in either treatment group. Conclusions: These results show that ALA preconditioning is capable of reducing inflammatory markers while decreasing early kidney dysfunction and clinical posttransplant pancreatitis.
Fil: Ambrosi, Nella Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Arrosagaray, Victoria. Instituto de Nefrologia de Buenos Aires; Argentina
Fil: Guerrieri, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Uva, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Nefrologia de Buenos Aires; Argentina
Fil: Petroni, Jorgelina. Instituto de Nefrologia de Buenos Aires; Argentina
Fil: Buonpensiere, Mónica. Instituto de Nefrologia de Buenos Aires; Argentina
Fil: Iovanna, Juan. Aix-Marseille Université. Institut Paoli-Calmettes. Centre de Recherche en Cancérologie de Marseille; Francia
Fil: León, Luis. Instituto de Nefrologia de Buenos Aires; Argentina
Fil: Incardona, Claudio. Gador; Argentina
Fil: Chuluyan, Hector Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Casadei, Domingo H.. Instituto de Nefrologia de Buenos Aires; Argentina
Materia
Α-Lipoic Acid
Trasplantation
Donor Receptor
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/14009
Acceder
id CONICETDig_1d0bb99fa5ca23cb8d270290e9c5ca97
oai_identifier_str oai:ri.conicet.gov.ar:11336/14009
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling α-lipoic acid protects the ischemia reperfusion injury in human simultaneous kidney-pancreas transplantation.Ambrosi, Nella GabrielaArrosagaray, VictoriaGuerrieri, DiegoUva, Pablo DanielPetroni, JorgelinaBuonpensiere, MónicaIovanna, JuanLeón, LuisIncardona, ClaudioChuluyan, Hector EduardoCasadei, Domingo H.Α-Lipoic AcidTrasplantationDonor Receptorhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Background: Multiple factors have been implicated in the process of ischemia-reperfusion injury (IRI) in organ transplantation. Among these factors, oxidative damage seems to initiate the injury. α-lipoic acid (ALA) is a potent antioxidant that is used in patients with diabetic polyneuropathy. The aim of the present study was to determine the effect of ALA in patients undergoing simultaneous kidney-pancreas transplant by evaluating the functional recovery of the graft and biochemical markers of IRI. Methods: Twenty-six patients were included in the following groups: (i) untreated control; (ii) donor and recipient (DR) ALA-treated, in which ALA was administered both to the deceased donor and to the recipients; and (iii) recipient ALA-treated group. The expression of inflammatory genes, as observed in biopsies taken at the end of surgery, as well as the serum cytokines, secretory leukocyte protease inhibitor, regenerating islet-derived protein 3β/pancreatitis-associated protein, amylase, lipase, glucose, and creatinine levels were quantified as markers of organ function. Results: The DR group showed high levels of TGFβ and low levels of C3 and TNFα in the kidneys, whereas high levels of C3 and heme oxygenase were identified in pancreas biopsies. Decreases in serum IL-8, IL-6, secretory leukocyte protease inhibitor, and regenerating islet-derived protein 3 β/pancreatitis-associated protein were observed after surgery in the DR group. Serum lipase and amylase were lower in the DR group than in the control and recipient groups. Early kidney dysfunction and clinical pancreatitis were higher in the control group than in either treatment group. Conclusions: These results show that ALA preconditioning is capable of reducing inflammatory markers while decreasing early kidney dysfunction and clinical posttransplant pancreatitis.Fil: Ambrosi, Nella Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Arrosagaray, Victoria. Instituto de Nefrologia de Buenos Aires; ArgentinaFil: Guerrieri, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Uva, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Nefrologia de Buenos Aires; ArgentinaFil: Petroni, Jorgelina. Instituto de Nefrologia de Buenos Aires; ArgentinaFil: Buonpensiere, Mónica. Instituto de Nefrologia de Buenos Aires; ArgentinaFil: Iovanna, Juan. Aix-Marseille Université. Institut Paoli-Calmettes. Centre de Recherche en Cancérologie de Marseille; FranciaFil: León, Luis. Instituto de Nefrologia de Buenos Aires; ArgentinaFil: Incardona, Claudio. Gador; ArgentinaFil: Chuluyan, Hector Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Casadei, Domingo H.. Instituto de Nefrologia de Buenos Aires; ArgentinaLippincott Williams2016-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/14009Ambrosi, Nella Gabriela; Arrosagaray, Victoria; Guerrieri, Diego; Uva, Pablo Daniel; Petroni, Jorgelina; et al.; α-lipoic acid protects the ischemia reperfusion injury in human simultaneous kidney-pancreas transplantation.; Lippincott Williams; Transplantation; 100; 4; 4-2016; 908-9150041-1337enginfo:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/doi/10.1097/TP.0000000000000981info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:11:34Zoai:ri.conicet.gov.ar:11336/14009instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:11:34.661CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv α-lipoic acid protects the ischemia reperfusion injury in human simultaneous kidney-pancreas transplantation.
title α-lipoic acid protects the ischemia reperfusion injury in human simultaneous kidney-pancreas transplantation.
spellingShingle α-lipoic acid protects the ischemia reperfusion injury in human simultaneous kidney-pancreas transplantation.
Ambrosi, Nella Gabriela
Α-Lipoic Acid
Trasplantation
Donor Receptor
title_short α-lipoic acid protects the ischemia reperfusion injury in human simultaneous kidney-pancreas transplantation.
title_full α-lipoic acid protects the ischemia reperfusion injury in human simultaneous kidney-pancreas transplantation.
title_fullStr α-lipoic acid protects the ischemia reperfusion injury in human simultaneous kidney-pancreas transplantation.
title_full_unstemmed α-lipoic acid protects the ischemia reperfusion injury in human simultaneous kidney-pancreas transplantation.
title_sort α-lipoic acid protects the ischemia reperfusion injury in human simultaneous kidney-pancreas transplantation.
dc.creator.none.fl_str_mv Ambrosi, Nella Gabriela
Arrosagaray, Victoria
Guerrieri, Diego
Uva, Pablo Daniel
Petroni, Jorgelina
Buonpensiere, Mónica
Iovanna, Juan
León, Luis
Incardona, Claudio
Chuluyan, Hector Eduardo
Casadei, Domingo H.
author Ambrosi, Nella Gabriela
author_facet Ambrosi, Nella Gabriela
Arrosagaray, Victoria
Guerrieri, Diego
Uva, Pablo Daniel
Petroni, Jorgelina
Buonpensiere, Mónica
Iovanna, Juan
León, Luis
Incardona, Claudio
Chuluyan, Hector Eduardo
Casadei, Domingo H.
author_role author
author2 Arrosagaray, Victoria
Guerrieri, Diego
Uva, Pablo Daniel
Petroni, Jorgelina
Buonpensiere, Mónica
Iovanna, Juan
León, Luis
Incardona, Claudio
Chuluyan, Hector Eduardo
Casadei, Domingo H.
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Α-Lipoic Acid
Trasplantation
Donor Receptor
topic Α-Lipoic Acid
Trasplantation
Donor Receptor
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background: Multiple factors have been implicated in the process of ischemia-reperfusion injury (IRI) in organ transplantation. Among these factors, oxidative damage seems to initiate the injury. α-lipoic acid (ALA) is a potent antioxidant that is used in patients with diabetic polyneuropathy. The aim of the present study was to determine the effect of ALA in patients undergoing simultaneous kidney-pancreas transplant by evaluating the functional recovery of the graft and biochemical markers of IRI. Methods: Twenty-six patients were included in the following groups: (i) untreated control; (ii) donor and recipient (DR) ALA-treated, in which ALA was administered both to the deceased donor and to the recipients; and (iii) recipient ALA-treated group. The expression of inflammatory genes, as observed in biopsies taken at the end of surgery, as well as the serum cytokines, secretory leukocyte protease inhibitor, regenerating islet-derived protein 3β/pancreatitis-associated protein, amylase, lipase, glucose, and creatinine levels were quantified as markers of organ function. Results: The DR group showed high levels of TGFβ and low levels of C3 and TNFα in the kidneys, whereas high levels of C3 and heme oxygenase were identified in pancreas biopsies. Decreases in serum IL-8, IL-6, secretory leukocyte protease inhibitor, and regenerating islet-derived protein 3 β/pancreatitis-associated protein were observed after surgery in the DR group. Serum lipase and amylase were lower in the DR group than in the control and recipient groups. Early kidney dysfunction and clinical pancreatitis were higher in the control group than in either treatment group. Conclusions: These results show that ALA preconditioning is capable of reducing inflammatory markers while decreasing early kidney dysfunction and clinical posttransplant pancreatitis.
Fil: Ambrosi, Nella Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Arrosagaray, Victoria. Instituto de Nefrologia de Buenos Aires; Argentina
Fil: Guerrieri, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Uva, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Nefrologia de Buenos Aires; Argentina
Fil: Petroni, Jorgelina. Instituto de Nefrologia de Buenos Aires; Argentina
Fil: Buonpensiere, Mónica. Instituto de Nefrologia de Buenos Aires; Argentina
Fil: Iovanna, Juan. Aix-Marseille Université. Institut Paoli-Calmettes. Centre de Recherche en Cancérologie de Marseille; Francia
Fil: León, Luis. Instituto de Nefrologia de Buenos Aires; Argentina
Fil: Incardona, Claudio. Gador; Argentina
Fil: Chuluyan, Hector Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Casadei, Domingo H.. Instituto de Nefrologia de Buenos Aires; Argentina
description Background: Multiple factors have been implicated in the process of ischemia-reperfusion injury (IRI) in organ transplantation. Among these factors, oxidative damage seems to initiate the injury. α-lipoic acid (ALA) is a potent antioxidant that is used in patients with diabetic polyneuropathy. The aim of the present study was to determine the effect of ALA in patients undergoing simultaneous kidney-pancreas transplant by evaluating the functional recovery of the graft and biochemical markers of IRI. Methods: Twenty-six patients were included in the following groups: (i) untreated control; (ii) donor and recipient (DR) ALA-treated, in which ALA was administered both to the deceased donor and to the recipients; and (iii) recipient ALA-treated group. The expression of inflammatory genes, as observed in biopsies taken at the end of surgery, as well as the serum cytokines, secretory leukocyte protease inhibitor, regenerating islet-derived protein 3β/pancreatitis-associated protein, amylase, lipase, glucose, and creatinine levels were quantified as markers of organ function. Results: The DR group showed high levels of TGFβ and low levels of C3 and TNFα in the kidneys, whereas high levels of C3 and heme oxygenase were identified in pancreas biopsies. Decreases in serum IL-8, IL-6, secretory leukocyte protease inhibitor, and regenerating islet-derived protein 3 β/pancreatitis-associated protein were observed after surgery in the DR group. Serum lipase and amylase were lower in the DR group than in the control and recipient groups. Early kidney dysfunction and clinical pancreatitis were higher in the control group than in either treatment group. Conclusions: These results show that ALA preconditioning is capable of reducing inflammatory markers while decreasing early kidney dysfunction and clinical posttransplant pancreatitis.
publishDate 2016
dc.date.none.fl_str_mv 2016-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/14009
Ambrosi, Nella Gabriela; Arrosagaray, Victoria; Guerrieri, Diego; Uva, Pablo Daniel; Petroni, Jorgelina; et al.; α-lipoic acid protects the ischemia reperfusion injury in human simultaneous kidney-pancreas transplantation.; Lippincott Williams; Transplantation; 100; 4; 4-2016; 908-915
0041-1337
url http://hdl.handle.net/11336/14009
identifier_str_mv Ambrosi, Nella Gabriela; Arrosagaray, Victoria; Guerrieri, Diego; Uva, Pablo Daniel; Petroni, Jorgelina; et al.; α-lipoic acid protects the ischemia reperfusion injury in human simultaneous kidney-pancreas transplantation.; Lippincott Williams; Transplantation; 100; 4; 4-2016; 908-915
0041-1337
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/
info:eu-repo/semantics/altIdentifier/doi/10.1097/TP.0000000000000981
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Lippincott Williams
publisher.none.fl_str_mv Lippincott Williams
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842270163636846592
score 13.13397

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