Genomic Analysis of two NDM-1 Providencia stuartii Strains Recovered from a Single Patient

Autores
Hoard, Amparo; Montaña, Sabrina Daiana; Moriano, Alessandro; Fernandez, Jennifer S.; Traglia, German Matias; Quiroga, Cecilia; Franchi, Agustina; Cohen, Emilia; Corigliano, Cecilia; Almuzara, Marisa; Ramirez, Maria Soledad
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In the last years, an increasing number of untreatable infections caused by drug-resistant microbes have impacted the health care system. Worldwide, infections caused by carbapenem-resistant (CR) Gram-negative bacilli have dramatically increased. Among the CR-Gram-negative bacilli, those producing carbapenemases, such as NDM-1, are the main concern. Different Enterobacterales harboring NDM-1 have been reported lately. Providencia stuartii, a member of the Morganellaceae family, is ubiquitous in the environment, but is also known to cause nosocomial infections. Here we describe the genomic analysis of two NDM-1- producing P. stuartii strains recovered from the same patient as well as other carbapenem resistant strains recovered from the same hospital. As a result of the genomic analysis thirteen resistance genes, including three to β-lactams (blaOXA-1, blaTEM-1, blaNDM-1), four to aminoglycosides (aphA6, aac(3)-IId, aac(2′)-Ia, aac(6′)-Ib-cr5), one to sulfonamides (sul1), two to chloramphenicol (catB3, catA3), one to rifampicin, one to bleomycin (ble), and one to tetracycline (tet(B)) were found. Moreover, a variety of mobile genetic elements, such as insertion sequences, plasmids and phage- related sequences, were found within P. stuartii genomes. The spread of carbapenem-resistant isolates remains a significant clinical and public health concern. Therefore, we considered that the detection of CR isolates is an essential step in addressing this problem.
Fil: Hoard, Amparo. California State University; Estados Unidos
Fil: Montaña, Sabrina Daiana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Moriano, Alessandro. California State University; Estados Unidos
Fil: Fernandez, Jennifer S.. California State University; Estados Unidos
Fil: Traglia, German Matias. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Quiroga, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Franchi, Agustina. Gobierno de la Provincia de Buenos Aires. Hospital Interzonal de Agudos Evita.; Argentina
Fil: Cohen, Emilia. Gobierno de la Provincia de Buenos Aires. Hospital Interzonal de Agudos Evita.; Argentina
Fil: Corigliano, Cecilia. Gobierno de la Provincia de Buenos Aires. Hospital Interzonal de Agudos Evita.; Argentina
Fil: Almuzara, Marisa. Gobierno de la Provincia de Buenos Aires. Hospital Interzonal de Agudos Evita.; Argentina
Fil: Ramirez, Maria Soledad. California State University; Estados Unidos
Materia
PROVIDENCIA
NDM-1
GENOME ANALYSIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/144702
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Genomic Analysis of two NDM-1 Providencia stuartii Strains Recovered from a Single PatientHoard, AmparoMontaña, Sabrina DaianaMoriano, AlessandroFernandez, Jennifer S.Traglia, German MatiasQuiroga, CeciliaFranchi, AgustinaCohen, EmiliaCorigliano, CeciliaAlmuzara, MarisaRamirez, Maria SoledadPROVIDENCIANDM-1GENOME ANALYSIShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In the last years, an increasing number of untreatable infections caused by drug-resistant microbes have impacted the health care system. Worldwide, infections caused by carbapenem-resistant (CR) Gram-negative bacilli have dramatically increased. Among the CR-Gram-negative bacilli, those producing carbapenemases, such as NDM-1, are the main concern. Different Enterobacterales harboring NDM-1 have been reported lately. Providencia stuartii, a member of the Morganellaceae family, is ubiquitous in the environment, but is also known to cause nosocomial infections. Here we describe the genomic analysis of two NDM-1- producing P. stuartii strains recovered from the same patient as well as other carbapenem resistant strains recovered from the same hospital. As a result of the genomic analysis thirteen resistance genes, including three to β-lactams (blaOXA-1, blaTEM-1, blaNDM-1), four to aminoglycosides (aphA6, aac(3)-IId, aac(2′)-Ia, aac(6′)-Ib-cr5), one to sulfonamides (sul1), two to chloramphenicol (catB3, catA3), one to rifampicin, one to bleomycin (ble), and one to tetracycline (tet(B)) were found. Moreover, a variety of mobile genetic elements, such as insertion sequences, plasmids and phage- related sequences, were found within P. stuartii genomes. The spread of carbapenem-resistant isolates remains a significant clinical and public health concern. Therefore, we considered that the detection of CR isolates is an essential step in addressing this problem.Fil: Hoard, Amparo. California State University; Estados UnidosFil: Montaña, Sabrina Daiana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; ArgentinaFil: Moriano, Alessandro. California State University; Estados UnidosFil: Fernandez, Jennifer S.. California State University; Estados UnidosFil: Traglia, German Matias. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; ArgentinaFil: Quiroga, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Franchi, Agustina. Gobierno de la Provincia de Buenos Aires. Hospital Interzonal de Agudos Evita.; ArgentinaFil: Cohen, Emilia. Gobierno de la Provincia de Buenos Aires. Hospital Interzonal de Agudos Evita.; ArgentinaFil: Corigliano, Cecilia. Gobierno de la Provincia de Buenos Aires. Hospital Interzonal de Agudos Evita.; ArgentinaFil: Almuzara, Marisa. Gobierno de la Provincia de Buenos Aires. Hospital Interzonal de Agudos Evita.; ArgentinaFil: Ramirez, Maria Soledad. California State University; Estados UnidosSpringer2020-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/144702Hoard, Amparo; Montaña, Sabrina Daiana; Moriano, Alessandro; Fernandez, Jennifer S.; Traglia, German Matias; et al.; Genomic Analysis of two NDM-1 Providencia stuartii Strains Recovered from a Single Patient; Springer; Current Microbiology; 77; 12; 10-2020; 4029-40360343-8651CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://link.springer.com/10.1007/s00284-020-02242-6info:eu-repo/semantics/altIdentifier/doi/10.1007/s00284-020-02242-6info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:12:48Zoai:ri.conicet.gov.ar:11336/144702instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:12:48.282CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genomic Analysis of two NDM-1 Providencia stuartii Strains Recovered from a Single Patient
title Genomic Analysis of two NDM-1 Providencia stuartii Strains Recovered from a Single Patient
spellingShingle Genomic Analysis of two NDM-1 Providencia stuartii Strains Recovered from a Single Patient
Hoard, Amparo
PROVIDENCIA
NDM-1
GENOME ANALYSIS
title_short Genomic Analysis of two NDM-1 Providencia stuartii Strains Recovered from a Single Patient
title_full Genomic Analysis of two NDM-1 Providencia stuartii Strains Recovered from a Single Patient
title_fullStr Genomic Analysis of two NDM-1 Providencia stuartii Strains Recovered from a Single Patient
title_full_unstemmed Genomic Analysis of two NDM-1 Providencia stuartii Strains Recovered from a Single Patient
title_sort Genomic Analysis of two NDM-1 Providencia stuartii Strains Recovered from a Single Patient
dc.creator.none.fl_str_mv Hoard, Amparo
Montaña, Sabrina Daiana
Moriano, Alessandro
Fernandez, Jennifer S.
Traglia, German Matias
Quiroga, Cecilia
Franchi, Agustina
Cohen, Emilia
Corigliano, Cecilia
Almuzara, Marisa
Ramirez, Maria Soledad
author Hoard, Amparo
author_facet Hoard, Amparo
Montaña, Sabrina Daiana
Moriano, Alessandro
Fernandez, Jennifer S.
Traglia, German Matias
Quiroga, Cecilia
Franchi, Agustina
Cohen, Emilia
Corigliano, Cecilia
Almuzara, Marisa
Ramirez, Maria Soledad
author_role author
author2 Montaña, Sabrina Daiana
Moriano, Alessandro
Fernandez, Jennifer S.
Traglia, German Matias
Quiroga, Cecilia
Franchi, Agustina
Cohen, Emilia
Corigliano, Cecilia
Almuzara, Marisa
Ramirez, Maria Soledad
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv PROVIDENCIA
NDM-1
GENOME ANALYSIS
topic PROVIDENCIA
NDM-1
GENOME ANALYSIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In the last years, an increasing number of untreatable infections caused by drug-resistant microbes have impacted the health care system. Worldwide, infections caused by carbapenem-resistant (CR) Gram-negative bacilli have dramatically increased. Among the CR-Gram-negative bacilli, those producing carbapenemases, such as NDM-1, are the main concern. Different Enterobacterales harboring NDM-1 have been reported lately. Providencia stuartii, a member of the Morganellaceae family, is ubiquitous in the environment, but is also known to cause nosocomial infections. Here we describe the genomic analysis of two NDM-1- producing P. stuartii strains recovered from the same patient as well as other carbapenem resistant strains recovered from the same hospital. As a result of the genomic analysis thirteen resistance genes, including three to β-lactams (blaOXA-1, blaTEM-1, blaNDM-1), four to aminoglycosides (aphA6, aac(3)-IId, aac(2′)-Ia, aac(6′)-Ib-cr5), one to sulfonamides (sul1), two to chloramphenicol (catB3, catA3), one to rifampicin, one to bleomycin (ble), and one to tetracycline (tet(B)) were found. Moreover, a variety of mobile genetic elements, such as insertion sequences, plasmids and phage- related sequences, were found within P. stuartii genomes. The spread of carbapenem-resistant isolates remains a significant clinical and public health concern. Therefore, we considered that the detection of CR isolates is an essential step in addressing this problem.
Fil: Hoard, Amparo. California State University; Estados Unidos
Fil: Montaña, Sabrina Daiana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Moriano, Alessandro. California State University; Estados Unidos
Fil: Fernandez, Jennifer S.. California State University; Estados Unidos
Fil: Traglia, German Matias. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Quiroga, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Franchi, Agustina. Gobierno de la Provincia de Buenos Aires. Hospital Interzonal de Agudos Evita.; Argentina
Fil: Cohen, Emilia. Gobierno de la Provincia de Buenos Aires. Hospital Interzonal de Agudos Evita.; Argentina
Fil: Corigliano, Cecilia. Gobierno de la Provincia de Buenos Aires. Hospital Interzonal de Agudos Evita.; Argentina
Fil: Almuzara, Marisa. Gobierno de la Provincia de Buenos Aires. Hospital Interzonal de Agudos Evita.; Argentina
Fil: Ramirez, Maria Soledad. California State University; Estados Unidos
description In the last years, an increasing number of untreatable infections caused by drug-resistant microbes have impacted the health care system. Worldwide, infections caused by carbapenem-resistant (CR) Gram-negative bacilli have dramatically increased. Among the CR-Gram-negative bacilli, those producing carbapenemases, such as NDM-1, are the main concern. Different Enterobacterales harboring NDM-1 have been reported lately. Providencia stuartii, a member of the Morganellaceae family, is ubiquitous in the environment, but is also known to cause nosocomial infections. Here we describe the genomic analysis of two NDM-1- producing P. stuartii strains recovered from the same patient as well as other carbapenem resistant strains recovered from the same hospital. As a result of the genomic analysis thirteen resistance genes, including three to β-lactams (blaOXA-1, blaTEM-1, blaNDM-1), four to aminoglycosides (aphA6, aac(3)-IId, aac(2′)-Ia, aac(6′)-Ib-cr5), one to sulfonamides (sul1), two to chloramphenicol (catB3, catA3), one to rifampicin, one to bleomycin (ble), and one to tetracycline (tet(B)) were found. Moreover, a variety of mobile genetic elements, such as insertion sequences, plasmids and phage- related sequences, were found within P. stuartii genomes. The spread of carbapenem-resistant isolates remains a significant clinical and public health concern. Therefore, we considered that the detection of CR isolates is an essential step in addressing this problem.
publishDate 2020
dc.date.none.fl_str_mv 2020-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/144702
Hoard, Amparo; Montaña, Sabrina Daiana; Moriano, Alessandro; Fernandez, Jennifer S.; Traglia, German Matias; et al.; Genomic Analysis of two NDM-1 Providencia stuartii Strains Recovered from a Single Patient; Springer; Current Microbiology; 77; 12; 10-2020; 4029-4036
0343-8651
CONICET Digital
CONICET
url http://hdl.handle.net/11336/144702
identifier_str_mv Hoard, Amparo; Montaña, Sabrina Daiana; Moriano, Alessandro; Fernandez, Jennifer S.; Traglia, German Matias; et al.; Genomic Analysis of two NDM-1 Providencia stuartii Strains Recovered from a Single Patient; Springer; Current Microbiology; 77; 12; 10-2020; 4029-4036
0343-8651
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/doi/10.1007/s00284-020-02242-6
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
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dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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