Different responses in Mandarin cultivars uncover a role of cuticular waxes in the resistance to citrus canker

Autores
Favaro, María Alejandra; Molina, Maria Celeste; Roeschlin, Roxana Andrea; Gadea, José; Gariglio, Norberto Francisco; Marano, María Rosa
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
'Okitsu' is a mandarin cultivar showing substantial resistance to X. citri subsp. citri (X. citri). We have previously shown that this cultivar has significantly lower canker incidence and severity than 'Clemenules', particularly during early stages of leaf development in the field. This differential response is only seen when the leaves are inoculated by spraying, suggesting that leaf surface contributes to resistance. In this work, we have studied structural and chemical properties of leaf surface barriers of both cultivars. Ultrastructural analysis showed a thicker cuticle covering epidermal surface and guard cells in young 'Okitsu' leaves than in 'Clemenules'. This thicker cuticle was associated with a smaller stomatal aperture and reduced cuticle permeability. These findings correlated with an accumulation of cuticular wax components, including primary alcohols, alkanes, and fatty acids. None of these differences were observed in mature leaves, where both cultivars are equally resistant to the bacterium. Remarkably, mechanical alteration of cuticular thickness of young 'Okitsu' leaves allows canker development. Furthermore, cuticular waxes extracted from young 'Okitsu' leaves have higher antibacterial activity against X. citri than 'Clemenules'. Taken together, these data suggest that a faster development of epicuticular waxes in 'Okitsu' leaves play a central role in its resistance to X. citri.
Fil: Favaro, María Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Ciencias Agropecuarias del Litoral. - Universidad Nacional del Litoral. Instituto de Ciencias Agropecuarias del Litoral.; Argentina
Fil: Molina, Maria Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Roeschlin, Roxana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Gadea, José. Instituto de Biologia Molecular y Celular de Plantas; España
Fil: Gariglio, Norberto Francisco. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Ciencias Agropecuarias del Litoral. - Universidad Nacional del Litoral. Instituto de Ciencias Agropecuarias del Litoral.; Argentina
Fil: Marano, María Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Materia
'OKITSU' MANDARIN
BACTERIOLOGY
CANKER DISEASE RESISTANCE
CUTICLE
CUTICULAR THICKNESS
GENETICS AND RESISTANCE
STOMATAL DEFENSE
WAXES
XANTHOMONAS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/151039
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/151039
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Different responses in Mandarin cultivars uncover a role of cuticular waxes in the resistance to citrus cankerFavaro, María AlejandraMolina, Maria CelesteRoeschlin, Roxana AndreaGadea, JoséGariglio, Norberto FranciscoMarano, María Rosa'OKITSU' MANDARINBACTERIOLOGYCANKER DISEASE RESISTANCECUTICLECUTICULAR THICKNESSGENETICS AND RESISTANCESTOMATAL DEFENSEWAXESXANTHOMONAShttps://purl.org/becyt/ford/4.5https://purl.org/becyt/ford/4'Okitsu' is a mandarin cultivar showing substantial resistance to X. citri subsp. citri (X. citri). We have previously shown that this cultivar has significantly lower canker incidence and severity than 'Clemenules', particularly during early stages of leaf development in the field. This differential response is only seen when the leaves are inoculated by spraying, suggesting that leaf surface contributes to resistance. In this work, we have studied structural and chemical properties of leaf surface barriers of both cultivars. Ultrastructural analysis showed a thicker cuticle covering epidermal surface and guard cells in young 'Okitsu' leaves than in 'Clemenules'. This thicker cuticle was associated with a smaller stomatal aperture and reduced cuticle permeability. These findings correlated with an accumulation of cuticular wax components, including primary alcohols, alkanes, and fatty acids. None of these differences were observed in mature leaves, where both cultivars are equally resistant to the bacterium. Remarkably, mechanical alteration of cuticular thickness of young 'Okitsu' leaves allows canker development. Furthermore, cuticular waxes extracted from young 'Okitsu' leaves have higher antibacterial activity against X. citri than 'Clemenules'. Taken together, these data suggest that a faster development of epicuticular waxes in 'Okitsu' leaves play a central role in its resistance to X. citri.Fil: Favaro, María Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Ciencias Agropecuarias del Litoral. - Universidad Nacional del Litoral. Instituto de Ciencias Agropecuarias del Litoral.; ArgentinaFil: Molina, Maria Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Roeschlin, Roxana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Gadea, José. Instituto de Biologia Molecular y Celular de Plantas; EspañaFil: Gariglio, Norberto Francisco. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Ciencias Agropecuarias del Litoral. - Universidad Nacional del Litoral. Instituto de Ciencias Agropecuarias del Litoral.; ArgentinaFil: Marano, María Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaAmerican Phytopathological Society2020-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/zipapplication/pdfhttp://hdl.handle.net/11336/151039Favaro, María Alejandra; Molina, Maria Celeste; Roeschlin, Roxana Andrea; Gadea, José; Gariglio, Norberto Francisco; et al.; Different responses in Mandarin cultivars uncover a role of cuticular waxes in the resistance to citrus canker; American Phytopathological Society; Phytopathology; 110; 11; 6-2020; 1791-18010031-949XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://apsjournals.apsnet.org/doi/10.1094/PHYTO-02-20-0053-Rinfo:eu-repo/semantics/altIdentifier/doi/10.1094/PHYTO-02-20-0053-Rinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:36:04Zoai:ri.conicet.gov.ar:11336/151039instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:36:05.18CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Different responses in Mandarin cultivars uncover a role of cuticular waxes in the resistance to citrus canker
title Different responses in Mandarin cultivars uncover a role of cuticular waxes in the resistance to citrus canker
spellingShingle Different responses in Mandarin cultivars uncover a role of cuticular waxes in the resistance to citrus canker
Favaro, María Alejandra
'OKITSU' MANDARIN
BACTERIOLOGY
CANKER DISEASE RESISTANCE
CUTICLE
CUTICULAR THICKNESS
GENETICS AND RESISTANCE
STOMATAL DEFENSE
WAXES
XANTHOMONAS
title_short Different responses in Mandarin cultivars uncover a role of cuticular waxes in the resistance to citrus canker
title_full Different responses in Mandarin cultivars uncover a role of cuticular waxes in the resistance to citrus canker
title_fullStr Different responses in Mandarin cultivars uncover a role of cuticular waxes in the resistance to citrus canker
title_full_unstemmed Different responses in Mandarin cultivars uncover a role of cuticular waxes in the resistance to citrus canker
title_sort Different responses in Mandarin cultivars uncover a role of cuticular waxes in the resistance to citrus canker
dc.creator.none.fl_str_mv Favaro, María Alejandra
Molina, Maria Celeste
Roeschlin, Roxana Andrea
Gadea, José
Gariglio, Norberto Francisco
Marano, María Rosa
author Favaro, María Alejandra
author_facet Favaro, María Alejandra
Molina, Maria Celeste
Roeschlin, Roxana Andrea
Gadea, José
Gariglio, Norberto Francisco
Marano, María Rosa
author_role author
author2 Molina, Maria Celeste
Roeschlin, Roxana Andrea
Gadea, José
Gariglio, Norberto Francisco
Marano, María Rosa
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv 'OKITSU' MANDARIN
BACTERIOLOGY
CANKER DISEASE RESISTANCE
CUTICLE
CUTICULAR THICKNESS
GENETICS AND RESISTANCE
STOMATAL DEFENSE
WAXES
XANTHOMONAS
topic 'OKITSU' MANDARIN
BACTERIOLOGY
CANKER DISEASE RESISTANCE
CUTICLE
CUTICULAR THICKNESS
GENETICS AND RESISTANCE
STOMATAL DEFENSE
WAXES
XANTHOMONAS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.5
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv 'Okitsu' is a mandarin cultivar showing substantial resistance to X. citri subsp. citri (X. citri). We have previously shown that this cultivar has significantly lower canker incidence and severity than 'Clemenules', particularly during early stages of leaf development in the field. This differential response is only seen when the leaves are inoculated by spraying, suggesting that leaf surface contributes to resistance. In this work, we have studied structural and chemical properties of leaf surface barriers of both cultivars. Ultrastructural analysis showed a thicker cuticle covering epidermal surface and guard cells in young 'Okitsu' leaves than in 'Clemenules'. This thicker cuticle was associated with a smaller stomatal aperture and reduced cuticle permeability. These findings correlated with an accumulation of cuticular wax components, including primary alcohols, alkanes, and fatty acids. None of these differences were observed in mature leaves, where both cultivars are equally resistant to the bacterium. Remarkably, mechanical alteration of cuticular thickness of young 'Okitsu' leaves allows canker development. Furthermore, cuticular waxes extracted from young 'Okitsu' leaves have higher antibacterial activity against X. citri than 'Clemenules'. Taken together, these data suggest that a faster development of epicuticular waxes in 'Okitsu' leaves play a central role in its resistance to X. citri.
Fil: Favaro, María Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Ciencias Agropecuarias del Litoral. - Universidad Nacional del Litoral. Instituto de Ciencias Agropecuarias del Litoral.; Argentina
Fil: Molina, Maria Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Roeschlin, Roxana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Gadea, José. Instituto de Biologia Molecular y Celular de Plantas; España
Fil: Gariglio, Norberto Francisco. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Ciencias Agropecuarias del Litoral. - Universidad Nacional del Litoral. Instituto de Ciencias Agropecuarias del Litoral.; Argentina
Fil: Marano, María Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
description 'Okitsu' is a mandarin cultivar showing substantial resistance to X. citri subsp. citri (X. citri). We have previously shown that this cultivar has significantly lower canker incidence and severity than 'Clemenules', particularly during early stages of leaf development in the field. This differential response is only seen when the leaves are inoculated by spraying, suggesting that leaf surface contributes to resistance. In this work, we have studied structural and chemical properties of leaf surface barriers of both cultivars. Ultrastructural analysis showed a thicker cuticle covering epidermal surface and guard cells in young 'Okitsu' leaves than in 'Clemenules'. This thicker cuticle was associated with a smaller stomatal aperture and reduced cuticle permeability. These findings correlated with an accumulation of cuticular wax components, including primary alcohols, alkanes, and fatty acids. None of these differences were observed in mature leaves, where both cultivars are equally resistant to the bacterium. Remarkably, mechanical alteration of cuticular thickness of young 'Okitsu' leaves allows canker development. Furthermore, cuticular waxes extracted from young 'Okitsu' leaves have higher antibacterial activity against X. citri than 'Clemenules'. Taken together, these data suggest that a faster development of epicuticular waxes in 'Okitsu' leaves play a central role in its resistance to X. citri.
publishDate 2020
dc.date.none.fl_str_mv 2020-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/151039
Favaro, María Alejandra; Molina, Maria Celeste; Roeschlin, Roxana Andrea; Gadea, José; Gariglio, Norberto Francisco; et al.; Different responses in Mandarin cultivars uncover a role of cuticular waxes in the resistance to citrus canker; American Phytopathological Society; Phytopathology; 110; 11; 6-2020; 1791-1801
0031-949X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/151039
identifier_str_mv Favaro, María Alejandra; Molina, Maria Celeste; Roeschlin, Roxana Andrea; Gadea, José; Gariglio, Norberto Francisco; et al.; Different responses in Mandarin cultivars uncover a role of cuticular waxes in the resistance to citrus canker; American Phytopathological Society; Phytopathology; 110; 11; 6-2020; 1791-1801
0031-949X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://apsjournals.apsnet.org/doi/10.1094/PHYTO-02-20-0053-R
info:eu-repo/semantics/altIdentifier/doi/10.1094/PHYTO-02-20-0053-R
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/zip
application/pdf
dc.publisher.none.fl_str_mv American Phytopathological Society
publisher.none.fl_str_mv American Phytopathological Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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