First detection of hepatitis E virus in Central Argentina: Environmental and serological survey

Autores
Martinez Wassaf, Maribel Graciela; Pisano, María Belén; Barril, Patricia Angelica; Elbarcha, Osvaldo C.; Pinto, Marcelo A.; Oliveira, Jaqueline Mendes de; DiGiusto, Pablo; Nates, Silvia Viviana; Ré, Viviana Elizabeth
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: The hepatitis E virus (HEV) is an emergent causative agent of acute hepatitis worldwide, transmitted by fecal-oral route. In Argentina it is considered rare, so differential laboratory testing is not routinely performed. Besides, in Argentina's central area epidemiological and molecular characteristics of HEV are still unknown. Objectives: Provide evidence of local circulation of HEV by molecular detection on environmental samples and by serological survey in healthy adult population of Córdoba city, Argentina. Study design: Environmental surveillance was conducted in river and sewage samples collected between 2007 and 2009–2011. Viral detection was performed by RT-Nested PCR of ORF-1 and ORF-2 partial regions. Anti-HEV IgG was determined by EIA in 433 serum samples collected between 2009 and 2010. Results: HEV was detected in 6.3% of raw sewage samples and in 3.2% of riverine samples. Nucleotide sequencing analyses revealed that all isolates belonged to genotype 3, subtypes a, b and c. The prevalence of IgG anti-HEV was 4.4%. Seroprevalence increased with the age of the individuals (OR: 3.50; 95% CI 1.39–8.87; p = 0.0065) and, although the prevalence was higher in low income population, no statistical relation was found between anti-HEV and socioeconomic level. Conclusions: The environmental findings added to serological results, demonstrate that HEV circulates in central Argentina. Contamination of water with HEV could represent a route of transmission for local populations, which have a high number of susceptible individuals. This fact alerts local health care systems in order to include detection of HEV in the diagnostic algorithm of viral hepatitis.
Fil: Martinez Wassaf, Maribel Graciela. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología “Dr. J. M. Vanella”; Argentina. Universidad Catolica de Córdoba. Facultad de Ciencias Químicas; Argentina. Laboratorio LACE; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pisano, María Belén. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología “Dr. J. M. Vanella”; Argentina. Universidad Catolica de Córdoba. Facultad de Ciencias Químicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Barril, Patricia Angelica. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología “Dr. J. M. Vanella”; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Elbarcha, Osvaldo C.. Laboratorio LACE; Argentina. Universidad Catolica de Córdoba. Facultad de Ciencias Químicas; Argentina
Fil: Pinto, Marcelo A.. Instituto Oswaldo Cruz; Brasil
Fil: Oliveira, Jaqueline Mendes de. Instituto Oswaldo Cruz; Brasil
Fil: DiGiusto, Pablo. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología “Dr. J. M. Vanella”; Argentina
Fil: Nates, Silvia Viviana. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología “Dr. J. M. Vanella”; Argentina
Fil: Ré, Viviana Elizabeth. Universidad Catolica de Córdoba. Facultad de Ciencias Químicas; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología “Dr. J. M. Vanella”; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Hepatitis e Virus
Environmental Surveillance
Seroprevalence
Molecular Epidemiology
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/34757
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/34757
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling First detection of hepatitis E virus in Central Argentina: Environmental and serological surveyMartinez Wassaf, Maribel GracielaPisano, María BelénBarril, Patricia AngelicaElbarcha, Osvaldo C.Pinto, Marcelo A.Oliveira, Jaqueline Mendes deDiGiusto, PabloNates, Silvia VivianaRé, Viviana ElizabethHepatitis e VirusEnvironmental SurveillanceSeroprevalenceMolecular Epidemiologyhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background: The hepatitis E virus (HEV) is an emergent causative agent of acute hepatitis worldwide, transmitted by fecal-oral route. In Argentina it is considered rare, so differential laboratory testing is not routinely performed. Besides, in Argentina's central area epidemiological and molecular characteristics of HEV are still unknown. Objectives: Provide evidence of local circulation of HEV by molecular detection on environmental samples and by serological survey in healthy adult population of Córdoba city, Argentina. Study design: Environmental surveillance was conducted in river and sewage samples collected between 2007 and 2009–2011. Viral detection was performed by RT-Nested PCR of ORF-1 and ORF-2 partial regions. Anti-HEV IgG was determined by EIA in 433 serum samples collected between 2009 and 2010. Results: HEV was detected in 6.3% of raw sewage samples and in 3.2% of riverine samples. Nucleotide sequencing analyses revealed that all isolates belonged to genotype 3, subtypes a, b and c. The prevalence of IgG anti-HEV was 4.4%. Seroprevalence increased with the age of the individuals (OR: 3.50; 95% CI 1.39–8.87; p = 0.0065) and, although the prevalence was higher in low income population, no statistical relation was found between anti-HEV and socioeconomic level. Conclusions: The environmental findings added to serological results, demonstrate that HEV circulates in central Argentina. Contamination of water with HEV could represent a route of transmission for local populations, which have a high number of susceptible individuals. This fact alerts local health care systems in order to include detection of HEV in the diagnostic algorithm of viral hepatitis.Fil: Martinez Wassaf, Maribel Graciela. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología “Dr. J. M. Vanella”; Argentina. Universidad Catolica de Córdoba. Facultad de Ciencias Químicas; Argentina. Laboratorio LACE; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pisano, María Belén. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología “Dr. J. M. Vanella”; Argentina. Universidad Catolica de Córdoba. Facultad de Ciencias Químicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Barril, Patricia Angelica. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología “Dr. J. M. Vanella”; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Elbarcha, Osvaldo C.. Laboratorio LACE; Argentina. Universidad Catolica de Córdoba. Facultad de Ciencias Químicas; ArgentinaFil: Pinto, Marcelo A.. Instituto Oswaldo Cruz; BrasilFil: Oliveira, Jaqueline Mendes de. Instituto Oswaldo Cruz; BrasilFil: DiGiusto, Pablo. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología “Dr. J. M. Vanella”; ArgentinaFil: Nates, Silvia Viviana. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología “Dr. J. M. Vanella”; ArgentinaFil: Ré, Viviana Elizabeth. Universidad Catolica de Córdoba. Facultad de Ciencias Químicas; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología “Dr. J. M. Vanella”; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier Science2014-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/34757Martinez Wassaf, Maribel Graciela; Pisano, María Belén; Barril, Patricia Angelica; Elbarcha, Osvaldo C.; Pinto, Marcelo A.; et al.; First detection of hepatitis E virus in Central Argentina: Environmental and serological survey; Elsevier Science; Journal of Clinical Virology; 61; 3; 8-2014; 334-3391386-6532CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.jcv.2014.08.016info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1386653214003163info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:55:31Zoai:ri.conicet.gov.ar:11336/34757instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:55:31.698CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv First detection of hepatitis E virus in Central Argentina: Environmental and serological survey
title First detection of hepatitis E virus in Central Argentina: Environmental and serological survey
spellingShingle First detection of hepatitis E virus in Central Argentina: Environmental and serological survey
Martinez Wassaf, Maribel Graciela
Hepatitis e Virus
Environmental Surveillance
Seroprevalence
Molecular Epidemiology
title_short First detection of hepatitis E virus in Central Argentina: Environmental and serological survey
title_full First detection of hepatitis E virus in Central Argentina: Environmental and serological survey
title_fullStr First detection of hepatitis E virus in Central Argentina: Environmental and serological survey
title_full_unstemmed First detection of hepatitis E virus in Central Argentina: Environmental and serological survey
title_sort First detection of hepatitis E virus in Central Argentina: Environmental and serological survey
dc.creator.none.fl_str_mv Martinez Wassaf, Maribel Graciela
Pisano, María Belén
Barril, Patricia Angelica
Elbarcha, Osvaldo C.
Pinto, Marcelo A.
Oliveira, Jaqueline Mendes de
DiGiusto, Pablo
Nates, Silvia Viviana
Ré, Viviana Elizabeth
author Martinez Wassaf, Maribel Graciela
author_facet Martinez Wassaf, Maribel Graciela
Pisano, María Belén
Barril, Patricia Angelica
Elbarcha, Osvaldo C.
Pinto, Marcelo A.
Oliveira, Jaqueline Mendes de
DiGiusto, Pablo
Nates, Silvia Viviana
Ré, Viviana Elizabeth
author_role author
author2 Pisano, María Belén
Barril, Patricia Angelica
Elbarcha, Osvaldo C.
Pinto, Marcelo A.
Oliveira, Jaqueline Mendes de
DiGiusto, Pablo
Nates, Silvia Viviana
Ré, Viviana Elizabeth
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Hepatitis e Virus
Environmental Surveillance
Seroprevalence
Molecular Epidemiology
topic Hepatitis e Virus
Environmental Surveillance
Seroprevalence
Molecular Epidemiology
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background: The hepatitis E virus (HEV) is an emergent causative agent of acute hepatitis worldwide, transmitted by fecal-oral route. In Argentina it is considered rare, so differential laboratory testing is not routinely performed. Besides, in Argentina's central area epidemiological and molecular characteristics of HEV are still unknown. Objectives: Provide evidence of local circulation of HEV by molecular detection on environmental samples and by serological survey in healthy adult population of Córdoba city, Argentina. Study design: Environmental surveillance was conducted in river and sewage samples collected between 2007 and 2009–2011. Viral detection was performed by RT-Nested PCR of ORF-1 and ORF-2 partial regions. Anti-HEV IgG was determined by EIA in 433 serum samples collected between 2009 and 2010. Results: HEV was detected in 6.3% of raw sewage samples and in 3.2% of riverine samples. Nucleotide sequencing analyses revealed that all isolates belonged to genotype 3, subtypes a, b and c. The prevalence of IgG anti-HEV was 4.4%. Seroprevalence increased with the age of the individuals (OR: 3.50; 95% CI 1.39–8.87; p = 0.0065) and, although the prevalence was higher in low income population, no statistical relation was found between anti-HEV and socioeconomic level. Conclusions: The environmental findings added to serological results, demonstrate that HEV circulates in central Argentina. Contamination of water with HEV could represent a route of transmission for local populations, which have a high number of susceptible individuals. This fact alerts local health care systems in order to include detection of HEV in the diagnostic algorithm of viral hepatitis.
Fil: Martinez Wassaf, Maribel Graciela. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología “Dr. J. M. Vanella”; Argentina. Universidad Catolica de Córdoba. Facultad de Ciencias Químicas; Argentina. Laboratorio LACE; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pisano, María Belén. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología “Dr. J. M. Vanella”; Argentina. Universidad Catolica de Córdoba. Facultad de Ciencias Químicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Barril, Patricia Angelica. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología “Dr. J. M. Vanella”; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Elbarcha, Osvaldo C.. Laboratorio LACE; Argentina. Universidad Catolica de Córdoba. Facultad de Ciencias Químicas; Argentina
Fil: Pinto, Marcelo A.. Instituto Oswaldo Cruz; Brasil
Fil: Oliveira, Jaqueline Mendes de. Instituto Oswaldo Cruz; Brasil
Fil: DiGiusto, Pablo. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología “Dr. J. M. Vanella”; Argentina
Fil: Nates, Silvia Viviana. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología “Dr. J. M. Vanella”; Argentina
Fil: Ré, Viviana Elizabeth. Universidad Catolica de Córdoba. Facultad de Ciencias Químicas; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología “Dr. J. M. Vanella”; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Background: The hepatitis E virus (HEV) is an emergent causative agent of acute hepatitis worldwide, transmitted by fecal-oral route. In Argentina it is considered rare, so differential laboratory testing is not routinely performed. Besides, in Argentina's central area epidemiological and molecular characteristics of HEV are still unknown. Objectives: Provide evidence of local circulation of HEV by molecular detection on environmental samples and by serological survey in healthy adult population of Córdoba city, Argentina. Study design: Environmental surveillance was conducted in river and sewage samples collected between 2007 and 2009–2011. Viral detection was performed by RT-Nested PCR of ORF-1 and ORF-2 partial regions. Anti-HEV IgG was determined by EIA in 433 serum samples collected between 2009 and 2010. Results: HEV was detected in 6.3% of raw sewage samples and in 3.2% of riverine samples. Nucleotide sequencing analyses revealed that all isolates belonged to genotype 3, subtypes a, b and c. The prevalence of IgG anti-HEV was 4.4%. Seroprevalence increased with the age of the individuals (OR: 3.50; 95% CI 1.39–8.87; p = 0.0065) and, although the prevalence was higher in low income population, no statistical relation was found between anti-HEV and socioeconomic level. Conclusions: The environmental findings added to serological results, demonstrate that HEV circulates in central Argentina. Contamination of water with HEV could represent a route of transmission for local populations, which have a high number of susceptible individuals. This fact alerts local health care systems in order to include detection of HEV in the diagnostic algorithm of viral hepatitis.
publishDate 2014
dc.date.none.fl_str_mv 2014-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/34757
Martinez Wassaf, Maribel Graciela; Pisano, María Belén; Barril, Patricia Angelica; Elbarcha, Osvaldo C.; Pinto, Marcelo A.; et al.; First detection of hepatitis E virus in Central Argentina: Environmental and serological survey; Elsevier Science; Journal of Clinical Virology; 61; 3; 8-2014; 334-339
1386-6532
CONICET Digital
CONICET
url http://hdl.handle.net/11336/34757
identifier_str_mv Martinez Wassaf, Maribel Graciela; Pisano, María Belén; Barril, Patricia Angelica; Elbarcha, Osvaldo C.; Pinto, Marcelo A.; et al.; First detection of hepatitis E virus in Central Argentina: Environmental and serological survey; Elsevier Science; Journal of Clinical Virology; 61; 3; 8-2014; 334-339
1386-6532
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jcv.2014.08.016
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1386653214003163
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
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dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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