Antitumor effects of Freeze-Dried Robusta Coffee (Coffea canephora) extracts on breast cancer cell lines

Autores
Nigra, Ayelén Denise; de Almeida Bauer Guimarães, Deborah; Prucca, Cesar German; Freitas Silva, Otniel; Teodoro, Anderson J.; Gil, German Alejandro
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Coffee consumption is believed to have chemopreventive and chemotherapeutic effects and to contribute to preventing the development and progression of cancer. However, there is still controversy around these claims. As indicated in our previous works, diet can influence the risk of breast cancer. Intake of coffee is hypothesized to reduce this risk, but current scientific evidence is not conclusive. This work is aimed at studying the effects of Robusta coffee bean extract on cell viability, proliferation, and apoptosis of different human cancers, especially breast cancer cell lines. To this end, cell viability was evaluated by Alamar Blue in 2D and 3D models, the cell cycle by PI, apoptosis by annexin V, mitochondrial morphology, and functionality by mitoTracker, and colony formation capacity by the clonogenic assay. Green and dark coffee extract significantly reduced viability in human breast, colorectal, brain, and bone cancer cells. Coffee anticancer activity was clearly evidenced in MDA-MB-231 (ER-) and MCF-7 (ER+) breast cancer cells but not in the normal breast cell line. In addition, coffee extract induces an increase S phase and a decrease G2/M population in breast cancer cells, affected the mitochondrial morphology, and triggered apoptosis. MDA-MB-231 breast cancer cells lost their clonogenic capacity after treatment. The antitumor activity was demonstrated in both 2D and 3D culture cell models.
Fil: Nigra, Ayelén Denise. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: de Almeida Bauer Guimarães, Deborah. Universidade Federal do Rio de Janeiro; Brasil
Fil: Prucca, Cesar German. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Freitas Silva, Otniel. Universidade Federal do Rio de Janeiro; Brasil
Fil: Teodoro, Anderson J.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Gil, German Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Materia
BREAST
CANCER
COFFEE
CHEMOTHERAPEUTIC
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/184276
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/184276
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Antitumor effects of Freeze-Dried Robusta Coffee (Coffea canephora) extracts on breast cancer cell linesNigra, Ayelén Denisede Almeida Bauer Guimarães, DeborahPrucca, Cesar GermanFreitas Silva, OtnielTeodoro, Anderson J.Gil, German AlejandroBREASTCANCERCOFFEECHEMOTHERAPEUTIChttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Coffee consumption is believed to have chemopreventive and chemotherapeutic effects and to contribute to preventing the development and progression of cancer. However, there is still controversy around these claims. As indicated in our previous works, diet can influence the risk of breast cancer. Intake of coffee is hypothesized to reduce this risk, but current scientific evidence is not conclusive. This work is aimed at studying the effects of Robusta coffee bean extract on cell viability, proliferation, and apoptosis of different human cancers, especially breast cancer cell lines. To this end, cell viability was evaluated by Alamar Blue in 2D and 3D models, the cell cycle by PI, apoptosis by annexin V, mitochondrial morphology, and functionality by mitoTracker, and colony formation capacity by the clonogenic assay. Green and dark coffee extract significantly reduced viability in human breast, colorectal, brain, and bone cancer cells. Coffee anticancer activity was clearly evidenced in MDA-MB-231 (ER-) and MCF-7 (ER+) breast cancer cells but not in the normal breast cell line. In addition, coffee extract induces an increase S phase and a decrease G2/M population in breast cancer cells, affected the mitochondrial morphology, and triggered apoptosis. MDA-MB-231 breast cancer cells lost their clonogenic capacity after treatment. The antitumor activity was demonstrated in both 2D and 3D culture cell models.Fil: Nigra, Ayelén Denise. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaFil: de Almeida Bauer Guimarães, Deborah. Universidade Federal do Rio de Janeiro; BrasilFil: Prucca, Cesar German. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Freitas Silva, Otniel. Universidade Federal do Rio de Janeiro; BrasilFil: Teodoro, Anderson J.. Universidade Federal do Rio de Janeiro; BrasilFil: Gil, German Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaLandes Bioscience2021-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/184276Nigra, Ayelén Denise; de Almeida Bauer Guimarães, Deborah; Prucca, Cesar German; Freitas Silva, Otniel; Teodoro, Anderson J.; et al.; Antitumor effects of Freeze-Dried Robusta Coffee (Coffea canephora) extracts on breast cancer cell lines; Landes Bioscience; Oxidative Medicine and Cellular Longevity; 2021; 5-2021; 1-161942-09001942-0994CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/omcl/2021/5572630/info:eu-repo/semantics/altIdentifier/doi/10.1155/2021/5572630info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:49:09Zoai:ri.conicet.gov.ar:11336/184276instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:49:09.438CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antitumor effects of Freeze-Dried Robusta Coffee (Coffea canephora) extracts on breast cancer cell lines
title Antitumor effects of Freeze-Dried Robusta Coffee (Coffea canephora) extracts on breast cancer cell lines
spellingShingle Antitumor effects of Freeze-Dried Robusta Coffee (Coffea canephora) extracts on breast cancer cell lines
Nigra, Ayelén Denise
BREAST
CANCER
COFFEE
CHEMOTHERAPEUTIC
title_short Antitumor effects of Freeze-Dried Robusta Coffee (Coffea canephora) extracts on breast cancer cell lines
title_full Antitumor effects of Freeze-Dried Robusta Coffee (Coffea canephora) extracts on breast cancer cell lines
title_fullStr Antitumor effects of Freeze-Dried Robusta Coffee (Coffea canephora) extracts on breast cancer cell lines
title_full_unstemmed Antitumor effects of Freeze-Dried Robusta Coffee (Coffea canephora) extracts on breast cancer cell lines
title_sort Antitumor effects of Freeze-Dried Robusta Coffee (Coffea canephora) extracts on breast cancer cell lines
dc.creator.none.fl_str_mv Nigra, Ayelén Denise
de Almeida Bauer Guimarães, Deborah
Prucca, Cesar German
Freitas Silva, Otniel
Teodoro, Anderson J.
Gil, German Alejandro
author Nigra, Ayelén Denise
author_facet Nigra, Ayelén Denise
de Almeida Bauer Guimarães, Deborah
Prucca, Cesar German
Freitas Silva, Otniel
Teodoro, Anderson J.
Gil, German Alejandro
author_role author
author2 de Almeida Bauer Guimarães, Deborah
Prucca, Cesar German
Freitas Silva, Otniel
Teodoro, Anderson J.
Gil, German Alejandro
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv BREAST
CANCER
COFFEE
CHEMOTHERAPEUTIC
topic BREAST
CANCER
COFFEE
CHEMOTHERAPEUTIC
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Coffee consumption is believed to have chemopreventive and chemotherapeutic effects and to contribute to preventing the development and progression of cancer. However, there is still controversy around these claims. As indicated in our previous works, diet can influence the risk of breast cancer. Intake of coffee is hypothesized to reduce this risk, but current scientific evidence is not conclusive. This work is aimed at studying the effects of Robusta coffee bean extract on cell viability, proliferation, and apoptosis of different human cancers, especially breast cancer cell lines. To this end, cell viability was evaluated by Alamar Blue in 2D and 3D models, the cell cycle by PI, apoptosis by annexin V, mitochondrial morphology, and functionality by mitoTracker, and colony formation capacity by the clonogenic assay. Green and dark coffee extract significantly reduced viability in human breast, colorectal, brain, and bone cancer cells. Coffee anticancer activity was clearly evidenced in MDA-MB-231 (ER-) and MCF-7 (ER+) breast cancer cells but not in the normal breast cell line. In addition, coffee extract induces an increase S phase and a decrease G2/M population in breast cancer cells, affected the mitochondrial morphology, and triggered apoptosis. MDA-MB-231 breast cancer cells lost their clonogenic capacity after treatment. The antitumor activity was demonstrated in both 2D and 3D culture cell models.
Fil: Nigra, Ayelén Denise. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: de Almeida Bauer Guimarães, Deborah. Universidade Federal do Rio de Janeiro; Brasil
Fil: Prucca, Cesar German. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Freitas Silva, Otniel. Universidade Federal do Rio de Janeiro; Brasil
Fil: Teodoro, Anderson J.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Gil, German Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
description Coffee consumption is believed to have chemopreventive and chemotherapeutic effects and to contribute to preventing the development and progression of cancer. However, there is still controversy around these claims. As indicated in our previous works, diet can influence the risk of breast cancer. Intake of coffee is hypothesized to reduce this risk, but current scientific evidence is not conclusive. This work is aimed at studying the effects of Robusta coffee bean extract on cell viability, proliferation, and apoptosis of different human cancers, especially breast cancer cell lines. To this end, cell viability was evaluated by Alamar Blue in 2D and 3D models, the cell cycle by PI, apoptosis by annexin V, mitochondrial morphology, and functionality by mitoTracker, and colony formation capacity by the clonogenic assay. Green and dark coffee extract significantly reduced viability in human breast, colorectal, brain, and bone cancer cells. Coffee anticancer activity was clearly evidenced in MDA-MB-231 (ER-) and MCF-7 (ER+) breast cancer cells but not in the normal breast cell line. In addition, coffee extract induces an increase S phase and a decrease G2/M population in breast cancer cells, affected the mitochondrial morphology, and triggered apoptosis. MDA-MB-231 breast cancer cells lost their clonogenic capacity after treatment. The antitumor activity was demonstrated in both 2D and 3D culture cell models.
publishDate 2021
dc.date.none.fl_str_mv 2021-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/184276
Nigra, Ayelén Denise; de Almeida Bauer Guimarães, Deborah; Prucca, Cesar German; Freitas Silva, Otniel; Teodoro, Anderson J.; et al.; Antitumor effects of Freeze-Dried Robusta Coffee (Coffea canephora) extracts on breast cancer cell lines; Landes Bioscience; Oxidative Medicine and Cellular Longevity; 2021; 5-2021; 1-16
1942-0900
1942-0994
CONICET Digital
CONICET
url http://hdl.handle.net/11336/184276
identifier_str_mv Nigra, Ayelén Denise; de Almeida Bauer Guimarães, Deborah; Prucca, Cesar German; Freitas Silva, Otniel; Teodoro, Anderson J.; et al.; Antitumor effects of Freeze-Dried Robusta Coffee (Coffea canephora) extracts on breast cancer cell lines; Landes Bioscience; Oxidative Medicine and Cellular Longevity; 2021; 5-2021; 1-16
1942-0900
1942-0994
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/omcl/2021/5572630/
info:eu-repo/semantics/altIdentifier/doi/10.1155/2021/5572630
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Landes Bioscience
publisher.none.fl_str_mv Landes Bioscience
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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