Effects of dietary conjugated linoleic acid at high-fat levels on triacylglycerol regulation in mice

Autores
Andreoli, Maria Florencia; González, Marcela Aída; Martinelli, Marcela Inés; Mocchiutti, Norberto Oscar; Bernal, Claudio Adrian
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objective: Our aim was to investigate the effects of dietary conjugated linoleic acid (CLA) athigh-fat (HF) levels on parameters related to triacylglycerol (TG) regulation and some potentialimpacts on liver damage.Methods: Growing mice were fed a control diet (7% corn oil), an HF diet containing 20% corn oil, or an HF diet containing 3% CLA (HF CLA) for 30 d. Tissue and organ weights, plasma and tissue TG levels, and parameters related to their regulation were evaluated. Liver oxidative status was also assessed.Results: Dietary CLA showed detrimental and beneficial effects. CLA added to the HF diet caused hepatomegaly (+32%) and exacerbated the hepatic TG accumulation (+168%) observed with the HF diet without inducing liver damage; however, it significantly reduced plasma TG concentrations (+37%) and normalized muscular TG content. An increase in glutathione was associated with total normalization of liver lipid peroxidation. In addition, HF + CLA caused dystrophy of epididymal fat pads, even when the HF diet had increased the adipose tissue mass (30%). The biochemical mechanisms involved in the regulation of lipid levels were related to reduced (-20%) hepatic very low-density lipoprotein-TG secretion and decreased muscle (-35%) and adipose (-49%) tissue contributions to the removal of plasma TG by lipoprotein lipase enzymes.Conclusion: Examination of CLA at HF levels showed hepatomegaly and exacerbation of lipidaccretion as a negative impact; however, some positive aspects such as hypotriglyceridemia andprotection against oxidative stress were also induced. Even the fat reduction is nutritionallyimportant for weight control; the biochemical mechanisms whereby CLA mediates the potentialeffects could produce undesirable metabolic alterations.
Fil: Andreoli, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Ciencias Biológicas. Cátedra de Bromatología y Nutrición; Argentina
Fil: González, Marcela Aída. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Ciencias Biológicas. Cátedra de Bromatología y Nutrición; Argentina
Fil: Martinelli, Marcela Inés. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Ciencias Biológicas. Cátedra de Bromatología y Nutrición; Argentina
Fil: Mocchiutti, Norberto Oscar. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Ciencias Biológicas. Cátedra de Bromatología y Nutrición; Argentina
Fil: Bernal, Claudio Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Ciencias Biológicas. Cátedra de Bromatología y Nutrición; Argentina
Materia
Conjugated linoleic acid
Triacylglycerol regulation
Nutrition
Oxidative status
Dietary fat
Isomeric fatty acids
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/102156
Acceder
id CONICETDig_2828446686411bb1fe715632ce24775e
oai_identifier_str oai:ri.conicet.gov.ar:11336/102156
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effects of dietary conjugated linoleic acid at high-fat levels on triacylglycerol regulation in miceAndreoli, Maria FlorenciaGonzález, Marcela AídaMartinelli, Marcela InésMocchiutti, Norberto OscarBernal, Claudio AdrianConjugated linoleic acidTriacylglycerol regulationNutritionOxidative statusDietary fatIsomeric fatty acidshttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Objective: Our aim was to investigate the effects of dietary conjugated linoleic acid (CLA) athigh-fat (HF) levels on parameters related to triacylglycerol (TG) regulation and some potentialimpacts on liver damage.Methods: Growing mice were fed a control diet (7% corn oil), an HF diet containing 20% corn oil, or an HF diet containing 3% CLA (HF CLA) for 30 d. Tissue and organ weights, plasma and tissue TG levels, and parameters related to their regulation were evaluated. Liver oxidative status was also assessed.Results: Dietary CLA showed detrimental and beneficial effects. CLA added to the HF diet caused hepatomegaly (+32%) and exacerbated the hepatic TG accumulation (+168%) observed with the HF diet without inducing liver damage; however, it significantly reduced plasma TG concentrations (+37%) and normalized muscular TG content. An increase in glutathione was associated with total normalization of liver lipid peroxidation. In addition, HF + CLA caused dystrophy of epididymal fat pads, even when the HF diet had increased the adipose tissue mass (30%). The biochemical mechanisms involved in the regulation of lipid levels were related to reduced (-20%) hepatic very low-density lipoprotein-TG secretion and decreased muscle (-35%) and adipose (-49%) tissue contributions to the removal of plasma TG by lipoprotein lipase enzymes.Conclusion: Examination of CLA at HF levels showed hepatomegaly and exacerbation of lipidaccretion as a negative impact; however, some positive aspects such as hypotriglyceridemia andprotection against oxidative stress were also induced. Even the fat reduction is nutritionallyimportant for weight control; the biochemical mechanisms whereby CLA mediates the potentialeffects could produce undesirable metabolic alterations.Fil: Andreoli, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Ciencias Biológicas. Cátedra de Bromatología y Nutrición; ArgentinaFil: González, Marcela Aída. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Ciencias Biológicas. Cátedra de Bromatología y Nutrición; ArgentinaFil: Martinelli, Marcela Inés. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Ciencias Biológicas. Cátedra de Bromatología y Nutrición; ArgentinaFil: Mocchiutti, Norberto Oscar. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Ciencias Biológicas. Cátedra de Bromatología y Nutrición; ArgentinaFil: Bernal, Claudio Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Ciencias Biológicas. Cátedra de Bromatología y Nutrición; ArgentinaElsevier Science Inc2009-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/102156Andreoli, Maria Florencia; González, Marcela Aída; Martinelli, Marcela Inés; Mocchiutti, Norberto Oscar; Bernal, Claudio Adrian; Effects of dietary conjugated linoleic acid at high-fat levels on triacylglycerol regulation in mice; Elsevier Science Inc; Nutrition; 25; 4; 4-2009; 445-4520899-9007CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0899900708004383info:eu-repo/semantics/altIdentifier/doi/10.1016/j.nut.2008.10.015info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:39:49Zoai:ri.conicet.gov.ar:11336/102156instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:39:49.518CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effects of dietary conjugated linoleic acid at high-fat levels on triacylglycerol regulation in mice
title Effects of dietary conjugated linoleic acid at high-fat levels on triacylglycerol regulation in mice
spellingShingle Effects of dietary conjugated linoleic acid at high-fat levels on triacylglycerol regulation in mice
Andreoli, Maria Florencia
Conjugated linoleic acid
Triacylglycerol regulation
Nutrition
Oxidative status
Dietary fat
Isomeric fatty acids
title_short Effects of dietary conjugated linoleic acid at high-fat levels on triacylglycerol regulation in mice
title_full Effects of dietary conjugated linoleic acid at high-fat levels on triacylglycerol regulation in mice
title_fullStr Effects of dietary conjugated linoleic acid at high-fat levels on triacylglycerol regulation in mice
title_full_unstemmed Effects of dietary conjugated linoleic acid at high-fat levels on triacylglycerol regulation in mice
title_sort Effects of dietary conjugated linoleic acid at high-fat levels on triacylglycerol regulation in mice
dc.creator.none.fl_str_mv Andreoli, Maria Florencia
González, Marcela Aída
Martinelli, Marcela Inés
Mocchiutti, Norberto Oscar
Bernal, Claudio Adrian
author Andreoli, Maria Florencia
author_facet Andreoli, Maria Florencia
González, Marcela Aída
Martinelli, Marcela Inés
Mocchiutti, Norberto Oscar
Bernal, Claudio Adrian
author_role author
author2 González, Marcela Aída
Martinelli, Marcela Inés
Mocchiutti, Norberto Oscar
Bernal, Claudio Adrian
author2_role author
author
author
author
dc.subject.none.fl_str_mv Conjugated linoleic acid
Triacylglycerol regulation
Nutrition
Oxidative status
Dietary fat
Isomeric fatty acids
topic Conjugated linoleic acid
Triacylglycerol regulation
Nutrition
Oxidative status
Dietary fat
Isomeric fatty acids
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Objective: Our aim was to investigate the effects of dietary conjugated linoleic acid (CLA) athigh-fat (HF) levels on parameters related to triacylglycerol (TG) regulation and some potentialimpacts on liver damage.Methods: Growing mice were fed a control diet (7% corn oil), an HF diet containing 20% corn oil, or an HF diet containing 3% CLA (HF CLA) for 30 d. Tissue and organ weights, plasma and tissue TG levels, and parameters related to their regulation were evaluated. Liver oxidative status was also assessed.Results: Dietary CLA showed detrimental and beneficial effects. CLA added to the HF diet caused hepatomegaly (+32%) and exacerbated the hepatic TG accumulation (+168%) observed with the HF diet without inducing liver damage; however, it significantly reduced plasma TG concentrations (+37%) and normalized muscular TG content. An increase in glutathione was associated with total normalization of liver lipid peroxidation. In addition, HF + CLA caused dystrophy of epididymal fat pads, even when the HF diet had increased the adipose tissue mass (30%). The biochemical mechanisms involved in the regulation of lipid levels were related to reduced (-20%) hepatic very low-density lipoprotein-TG secretion and decreased muscle (-35%) and adipose (-49%) tissue contributions to the removal of plasma TG by lipoprotein lipase enzymes.Conclusion: Examination of CLA at HF levels showed hepatomegaly and exacerbation of lipidaccretion as a negative impact; however, some positive aspects such as hypotriglyceridemia andprotection against oxidative stress were also induced. Even the fat reduction is nutritionallyimportant for weight control; the biochemical mechanisms whereby CLA mediates the potentialeffects could produce undesirable metabolic alterations.
Fil: Andreoli, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Ciencias Biológicas. Cátedra de Bromatología y Nutrición; Argentina
Fil: González, Marcela Aída. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Ciencias Biológicas. Cátedra de Bromatología y Nutrición; Argentina
Fil: Martinelli, Marcela Inés. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Ciencias Biológicas. Cátedra de Bromatología y Nutrición; Argentina
Fil: Mocchiutti, Norberto Oscar. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Ciencias Biológicas. Cátedra de Bromatología y Nutrición; Argentina
Fil: Bernal, Claudio Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Ciencias Biológicas. Cátedra de Bromatología y Nutrición; Argentina
description Objective: Our aim was to investigate the effects of dietary conjugated linoleic acid (CLA) athigh-fat (HF) levels on parameters related to triacylglycerol (TG) regulation and some potentialimpacts on liver damage.Methods: Growing mice were fed a control diet (7% corn oil), an HF diet containing 20% corn oil, or an HF diet containing 3% CLA (HF CLA) for 30 d. Tissue and organ weights, plasma and tissue TG levels, and parameters related to their regulation were evaluated. Liver oxidative status was also assessed.Results: Dietary CLA showed detrimental and beneficial effects. CLA added to the HF diet caused hepatomegaly (+32%) and exacerbated the hepatic TG accumulation (+168%) observed with the HF diet without inducing liver damage; however, it significantly reduced plasma TG concentrations (+37%) and normalized muscular TG content. An increase in glutathione was associated with total normalization of liver lipid peroxidation. In addition, HF + CLA caused dystrophy of epididymal fat pads, even when the HF diet had increased the adipose tissue mass (30%). The biochemical mechanisms involved in the regulation of lipid levels were related to reduced (-20%) hepatic very low-density lipoprotein-TG secretion and decreased muscle (-35%) and adipose (-49%) tissue contributions to the removal of plasma TG by lipoprotein lipase enzymes.Conclusion: Examination of CLA at HF levels showed hepatomegaly and exacerbation of lipidaccretion as a negative impact; however, some positive aspects such as hypotriglyceridemia andprotection against oxidative stress were also induced. Even the fat reduction is nutritionallyimportant for weight control; the biochemical mechanisms whereby CLA mediates the potentialeffects could produce undesirable metabolic alterations.
publishDate 2009
dc.date.none.fl_str_mv 2009-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/102156
Andreoli, Maria Florencia; González, Marcela Aída; Martinelli, Marcela Inés; Mocchiutti, Norberto Oscar; Bernal, Claudio Adrian; Effects of dietary conjugated linoleic acid at high-fat levels on triacylglycerol regulation in mice; Elsevier Science Inc; Nutrition; 25; 4; 4-2009; 445-452
0899-9007
CONICET Digital
CONICET
url http://hdl.handle.net/11336/102156
identifier_str_mv Andreoli, Maria Florencia; González, Marcela Aída; Martinelli, Marcela Inés; Mocchiutti, Norberto Oscar; Bernal, Claudio Adrian; Effects of dietary conjugated linoleic acid at high-fat levels on triacylglycerol regulation in mice; Elsevier Science Inc; Nutrition; 25; 4; 4-2009; 445-452
0899-9007
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0899900708004383
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.nut.2008.10.015
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Inc
publisher.none.fl_str_mv Elsevier Science Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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