Biosynthesized ZnO-NPs from Morus indica attenuates methylglyoxal-induced protein glycation and RBC damage: In-vitro, in-vivo and molecular docking study

• Autores: Anandan, Satish; Mahadevamurthy, Murali; Ansari, Mohammad Azam; Alzohairy, Mohammad A.; Alomary, Mohammad N.; Siraj, Syeda Farha; Nagaraja, Sarjan Halugudde; Chikkamadaiah, Mahendra; Ramachandrappa, Lakshmeesha Thimappa; Krishnappa, Hemanth Kumar Naguvanahalli; Ledesma, Ana Estela; Nagaraj, Amruthesh Kestur; Urooj, Asna
• Año de publicación: 2019
• Idioma: inglés
• Tipo de recurso: artículo
• Materia: glycation; in-vivo; diabetes; methylglyoxal; molecular docking; ribose; ZnO-NPs
• Institución: Consejo Nacional de Investigaciones Científicas y Técnicas

The development of advanced glycation end-products (AGEs) inhibitors is considered to have therapeutic potential in diabetic complications inhibiting the loss of the biomolecular function. In the present study, zinc oxide nanoparticles (ZnO-NPs) were synthesized from aqueous leaf extract of Morus indica and were characterized by various techniques such as ultraviolet (UV)-Vis spectroscopy, Powder X-Ray Diffraction (PXRD), Fourier Transform Infrared Spectroscopy (FT-IR), Scanning electron microscopy (SEM), and energy dispersive spectroscopy (EDS). Further, the inhibition of AGEs formation after exposure to ZnO-NPs was investigated by in-vitro, in-vivo, and molecular docking studies. Biochemical and histopathological changes after exposure to ZnO-NPs were also studied in streptozotocin-induced diabetic rats. ZnO-NPs showed an absorption peak at 359 nm with a purity of 92.62% and ~6–12 nm in size, which is characteristic of nanoparticles. The images of SEM showed agglomeration of smaller ZnO-NPs and EDS authenticating that the synthesized nanoparticles were without impurities. The biosynthesized ZnO-NPs showed significant inhibition in the formation of AGEs. The particles were effective against methylglyoxal (MGO) mediated glycation of bovine serum albumin (BSA) by inhibiting the formation of AGEs, which was dose-dependent. Further, the presence of MGO resulted in complete damage of biconcave red blood corpuscles (RBCs) to an irregular shape, whereas the morphological changes were prevented when they were treated with ZnO-NPs leading to the prevention of complications caused due to glycation. The administration of ZnO-NPs (100 mg Kg−1) in streptozotocin(STZ)-induced diabetic rats reversed hyperglycemia and significantly improved hepatic enzymes level and renal functionality, also the histopathological studies revealed restoration of kidney and liver damage nearer to normal conditions. Molecular docking of BSA with ZnO-NPs confirms that masking of lysine and arginine residues is one of the possible mechanisms responsible for the potent antiglycation activity of ZnO-NPs. The findings strongly suggest scope for exploring the therapeutic potential of diabetes-related complications.
Fil: Anandan, Satish. University of Mysore; India
Fil: Mahadevamurthy, Murali. University of Mysore; India
Fil: Ansari, Mohammad Azam. Imam Abdulrahman Bin Faisal University; Arabia Saudita
Fil: Alzohairy, Mohammad A.. Al Qassim University; Arabia Saudita
Fil: Alomary, Mohammad N.. King Abdulaziz City For Science And Technology; Arabia Saudita
Fil: Siraj, Syeda Farha. University of Mysore; India
Fil: Nagaraja, Sarjan Halugudde. University of Mysore; India
Fil: Chikkamadaiah, Mahendra. University of Mysore; India
Fil: Ramachandrappa, Lakshmeesha Thimappa. University of Mysore; India
Fil: Krishnappa, Hemanth Kumar Naguvanahalli. University of Mysore; India
Fil: Ledesma, Ana Estela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Centro de Investigación en Biofísica Aplicada y Alimentos. - Universidad Nacional de Santiago del Estero. Centro de Investigación en Biofísica Aplicada y Alimentos; Argentina
Fil: Nagaraj, Amruthesh Kestur. University of Mysore; India
Fil: Urooj, Asna. University of Mysore; India