Effect of electrical and chemical stimulation of the subthalamic nucleus on the release of striatal dopamine

Autores
Pazo, Jorge Horacio; Höcht, Christian; Barceló, Ana C.; Fillipini, Bárbara; Lomastro, María Julieta
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
High-frequency stimulation (HFS) of the subthalamic nucleus (STN) alleviates the cardinal symptoms of Parkinson’s disease, but the mechanisms underlying these clinical results remain to be clarified. The HFS of STN is associated with the release of dopamine (DA) in the striatum. This study examines possible mechanisms by which HFS–STN release DA. The experiments were performed in rats anesthetized with urethane. The STN was stimulated by electrical HF and chemical microinjections of an antagonist and an agonist of GABAA receptors, the bicuculline, and the muscimol, respectively. The extracellular striatal DA-DOPAC (3-4-dihydroxyphenilacetic acid) content was collected by means of intracerebral microdialysis cannula and analyzed with HPLC with an electrochemical detector. The HFS of STN and microinjection of bicuculline intrasubthalamic produced a significant increase of extracellular striatal DA, whereas DOPAC levels were unchanged. The microinjection of muscimol depresses spontaneous release of DA, without changes in DOPAC. The kainic acid lesion of the globus pallidus (GP) and the substantia nigra pars reticulata (SNr), ipsilateral to dialyzed striatum, did not modify the release of DA-DOPAC. These data provide evidence that the STN has a tonic action on the substantia nigra pars compacta (SNc), and the release of striatal DA by HFS–STN may be due to activation of the STN acting directly on SNc neurons.
Fil: Pazo, Jorge Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Neurofisiología; Argentina
Fil: Höcht, Christian. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Barceló, Ana C.. Universidad de Buenos Aires. Facultad de Odontología; Argentina
Fil: Fillipini, Bárbara. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Neurofisiología; Argentina
Fil: Lomastro, María Julieta. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Neurofisiología; Argentina
Materia
Striatal dopamine
Subthalamic nucleus
High-frequency stimulation
Bicuculline
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/280999
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/280999
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of electrical and chemical stimulation of the subthalamic nucleus on the release of striatal dopaminePazo, Jorge HoracioHöcht, ChristianBarceló, Ana C.Fillipini, BárbaraLomastro, María JulietaStriatal dopamineSubthalamic nucleusHigh-frequency stimulationBicucullinehttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3High-frequency stimulation (HFS) of the subthalamic nucleus (STN) alleviates the cardinal symptoms of Parkinson’s disease, but the mechanisms underlying these clinical results remain to be clarified. The HFS of STN is associated with the release of dopamine (DA) in the striatum. This study examines possible mechanisms by which HFS–STN release DA. The experiments were performed in rats anesthetized with urethane. The STN was stimulated by electrical HF and chemical microinjections of an antagonist and an agonist of GABAA receptors, the bicuculline, and the muscimol, respectively. The extracellular striatal DA-DOPAC (3-4-dihydroxyphenilacetic acid) content was collected by means of intracerebral microdialysis cannula and analyzed with HPLC with an electrochemical detector. The HFS of STN and microinjection of bicuculline intrasubthalamic produced a significant increase of extracellular striatal DA, whereas DOPAC levels were unchanged. The microinjection of muscimol depresses spontaneous release of DA, without changes in DOPAC. The kainic acid lesion of the globus pallidus (GP) and the substantia nigra pars reticulata (SNr), ipsilateral to dialyzed striatum, did not modify the release of DA-DOPAC. These data provide evidence that the STN has a tonic action on the substantia nigra pars compacta (SNc), and the release of striatal DA by HFS–STN may be due to activation of the STN acting directly on SNc neurons.Fil: Pazo, Jorge Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Neurofisiología; ArgentinaFil: Höcht, Christian. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Barceló, Ana C.. Universidad de Buenos Aires. Facultad de Odontología; ArgentinaFil: Fillipini, Bárbara. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Neurofisiología; ArgentinaFil: Lomastro, María Julieta. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Neurofisiología; ArgentinaWiley-liss, div John Wiley & Sons Inc.2010-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/280999Pazo, Jorge Horacio; Höcht, Christian; Barceló, Ana C.; Fillipini, Bárbara; Lomastro, María Julieta; Effect of electrical and chemical stimulation of the subthalamic nucleus on the release of striatal dopamine; Wiley-liss, div John Wiley & Sons Inc.; Synapse; 64; 12; 4-2010; 905-9150887-4476CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1002/syn.20809info:eu-repo/semantics/altIdentifier/doi/10.1002/syn.20809info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-02-26T10:29:53Zoai:ri.conicet.gov.ar:11336/280999instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-02-26 10:29:53.288CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of electrical and chemical stimulation of the subthalamic nucleus on the release of striatal dopamine
title Effect of electrical and chemical stimulation of the subthalamic nucleus on the release of striatal dopamine
spellingShingle Effect of electrical and chemical stimulation of the subthalamic nucleus on the release of striatal dopamine
Pazo, Jorge Horacio
Striatal dopamine
Subthalamic nucleus
High-frequency stimulation
Bicuculline
title_short Effect of electrical and chemical stimulation of the subthalamic nucleus on the release of striatal dopamine
title_full Effect of electrical and chemical stimulation of the subthalamic nucleus on the release of striatal dopamine
title_fullStr Effect of electrical and chemical stimulation of the subthalamic nucleus on the release of striatal dopamine
title_full_unstemmed Effect of electrical and chemical stimulation of the subthalamic nucleus on the release of striatal dopamine
title_sort Effect of electrical and chemical stimulation of the subthalamic nucleus on the release of striatal dopamine
dc.creator.none.fl_str_mv Pazo, Jorge Horacio
Höcht, Christian
Barceló, Ana C.
Fillipini, Bárbara
Lomastro, María Julieta
author Pazo, Jorge Horacio
author_facet Pazo, Jorge Horacio
Höcht, Christian
Barceló, Ana C.
Fillipini, Bárbara
Lomastro, María Julieta
author_role author
author2 Höcht, Christian
Barceló, Ana C.
Fillipini, Bárbara
Lomastro, María Julieta
author2_role author
author
author
author
dc.subject.none.fl_str_mv Striatal dopamine
Subthalamic nucleus
High-frequency stimulation
Bicuculline
topic Striatal dopamine
Subthalamic nucleus
High-frequency stimulation
Bicuculline
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv High-frequency stimulation (HFS) of the subthalamic nucleus (STN) alleviates the cardinal symptoms of Parkinson’s disease, but the mechanisms underlying these clinical results remain to be clarified. The HFS of STN is associated with the release of dopamine (DA) in the striatum. This study examines possible mechanisms by which HFS–STN release DA. The experiments were performed in rats anesthetized with urethane. The STN was stimulated by electrical HF and chemical microinjections of an antagonist and an agonist of GABAA receptors, the bicuculline, and the muscimol, respectively. The extracellular striatal DA-DOPAC (3-4-dihydroxyphenilacetic acid) content was collected by means of intracerebral microdialysis cannula and analyzed with HPLC with an electrochemical detector. The HFS of STN and microinjection of bicuculline intrasubthalamic produced a significant increase of extracellular striatal DA, whereas DOPAC levels were unchanged. The microinjection of muscimol depresses spontaneous release of DA, without changes in DOPAC. The kainic acid lesion of the globus pallidus (GP) and the substantia nigra pars reticulata (SNr), ipsilateral to dialyzed striatum, did not modify the release of DA-DOPAC. These data provide evidence that the STN has a tonic action on the substantia nigra pars compacta (SNc), and the release of striatal DA by HFS–STN may be due to activation of the STN acting directly on SNc neurons.
Fil: Pazo, Jorge Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Neurofisiología; Argentina
Fil: Höcht, Christian. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Barceló, Ana C.. Universidad de Buenos Aires. Facultad de Odontología; Argentina
Fil: Fillipini, Bárbara. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Neurofisiología; Argentina
Fil: Lomastro, María Julieta. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Neurofisiología; Argentina
description High-frequency stimulation (HFS) of the subthalamic nucleus (STN) alleviates the cardinal symptoms of Parkinson’s disease, but the mechanisms underlying these clinical results remain to be clarified. The HFS of STN is associated with the release of dopamine (DA) in the striatum. This study examines possible mechanisms by which HFS–STN release DA. The experiments were performed in rats anesthetized with urethane. The STN was stimulated by electrical HF and chemical microinjections of an antagonist and an agonist of GABAA receptors, the bicuculline, and the muscimol, respectively. The extracellular striatal DA-DOPAC (3-4-dihydroxyphenilacetic acid) content was collected by means of intracerebral microdialysis cannula and analyzed with HPLC with an electrochemical detector. The HFS of STN and microinjection of bicuculline intrasubthalamic produced a significant increase of extracellular striatal DA, whereas DOPAC levels were unchanged. The microinjection of muscimol depresses spontaneous release of DA, without changes in DOPAC. The kainic acid lesion of the globus pallidus (GP) and the substantia nigra pars reticulata (SNr), ipsilateral to dialyzed striatum, did not modify the release of DA-DOPAC. These data provide evidence that the STN has a tonic action on the substantia nigra pars compacta (SNc), and the release of striatal DA by HFS–STN may be due to activation of the STN acting directly on SNc neurons.
publishDate 2010
dc.date.none.fl_str_mv 2010-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/280999
Pazo, Jorge Horacio; Höcht, Christian; Barceló, Ana C.; Fillipini, Bárbara; Lomastro, María Julieta; Effect of electrical and chemical stimulation of the subthalamic nucleus on the release of striatal dopamine; Wiley-liss, div John Wiley & Sons Inc.; Synapse; 64; 12; 4-2010; 905-915
0887-4476
CONICET Digital
CONICET
url http://hdl.handle.net/11336/280999
identifier_str_mv Pazo, Jorge Horacio; Höcht, Christian; Barceló, Ana C.; Fillipini, Bárbara; Lomastro, María Julieta; Effect of electrical and chemical stimulation of the subthalamic nucleus on the release of striatal dopamine; Wiley-liss, div John Wiley & Sons Inc.; Synapse; 64; 12; 4-2010; 905-915
0887-4476
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1002/syn.20809
info:eu-repo/semantics/altIdentifier/doi/10.1002/syn.20809
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley-liss, div John Wiley & Sons Inc.
publisher.none.fl_str_mv Wiley-liss, div John Wiley & Sons Inc.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.665996

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