Hippocampal high-frequency stimulation inhibites the progression of rapid kindling-induced seizure in rats

Autores
Gori, María Belén; Pereyra, Magdalena; Toibaro, Lucas; Brescacin, Carola; Battaglia, Gerardo Ángel; Pastorino, Julieta; Smigliani, Ariela; Galardi, Milagros; Kochen, Silvia
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Epilepsy is one of the most common serious neurological disorders. Pharmacoresistant epilepsy patients are poorly con-trolled or their seizures are refractory to drug treatment. Resective surgery is frequently a promising therapy in this population, however, not all the patients meet the eligibility criteria for the surgical treatment. Deep brain stimulation has been investigated in clinical studies and animal studies as an alternative treatment, but the optimal stimulation pa- rameters remain an issue. The present study was designed to investigate the effect of unilateral high-frequency stimula- tion (HFS) of hippocampus on seizure development by using the hippocampal rapid kindling method (hRK) in rats, and compared the results with those of low-frequency stimulation previously published by our group. We used male Wistar rats implanted with electrodes in the ventral hippocampus. All rats underwent hRK (biphasic square wave pulses, 20 Hz for 10 seconds) during three consecutive days (twelve stimulations per day). The control group (hRK; n = 7) received only RK stimulus, while the treated group (HFS-hRK; n = 9) received also HFS (biphasic square wave pulses, 130 Hz for 30 seconds) immediately before the RK stimulus, during three consecutive days. At the end of behavioral testing 78% (p < 0.01) of the animals receiving HFS treatment were still not fully kindled staying in stages 0 - III (p < 0.01). HFS group needed a higher number of stimulations to achieve stage III (p < 0.05) with respect to control group. How- ever, no significant differences in the cumulative daily afterdischarge duration were observed. HFS did not present sig- nificant differences compared with LFS in any of studied parameters. The findings suggest that unilateral HFS applied on hippocampus effectively inhibited the epileptogenic process induced by hippocampal rapid kindling. According to the comparative results about hippocampal rapid kindled animals stimulated with HFS and LFS (5 Hz), we found no conclusive information on which treatment is most efficient.
Fil: Gori, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: Pereyra, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: Toibaro, Lucas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: Brescacin, Carola. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: Battaglia, Gerardo Ángel. Universidad Tecnológica Nacional; Argentina
Fil: Pastorino, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: Smigliani, Ariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: Galardi, Milagros. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: Kochen, Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Materia
HIPPOCAMPAL RAPID KINDLING
EPILEPSY
ELECTRICAL STIMULATION
HIGH-FREQUENCY STIMULATION
LOW-FREQUENCY STIMULATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/1257
Acceder
id CONICETDig_7264e03632f701411876dc1be6815643
oai_identifier_str oai:ri.conicet.gov.ar:11336/1257
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Hippocampal high-frequency stimulation inhibites the progression of rapid kindling-induced seizure in ratsGori, María BelénPereyra, MagdalenaToibaro, LucasBrescacin, CarolaBattaglia, Gerardo ÁngelPastorino, JulietaSmigliani, ArielaGalardi, MilagrosKochen, SilviaHIPPOCAMPAL RAPID KINDLINGEPILEPSYELECTRICAL STIMULATIONHIGH-FREQUENCY STIMULATIONLOW-FREQUENCY STIMULATIONhttps://purl.org/becyt/ford/3.5https://purl.org/becyt/ford/3Epilepsy is one of the most common serious neurological disorders. Pharmacoresistant epilepsy patients are poorly con-trolled or their seizures are refractory to drug treatment. Resective surgery is frequently a promising therapy in this population, however, not all the patients meet the eligibility criteria for the surgical treatment. Deep brain stimulation has been investigated in clinical studies and animal studies as an alternative treatment, but the optimal stimulation pa- rameters remain an issue. The present study was designed to investigate the effect of unilateral high-frequency stimula- tion (HFS) of hippocampus on seizure development by using the hippocampal rapid kindling method (hRK) in rats, and compared the results with those of low-frequency stimulation previously published by our group. We used male Wistar rats implanted with electrodes in the ventral hippocampus. All rats underwent hRK (biphasic square wave pulses, 20 Hz for 10 seconds) during three consecutive days (twelve stimulations per day). The control group (hRK; n = 7) received only RK stimulus, while the treated group (HFS-hRK; n = 9) received also HFS (biphasic square wave pulses, 130 Hz for 30 seconds) immediately before the RK stimulus, during three consecutive days. At the end of behavioral testing 78% (p < 0.01) of the animals receiving HFS treatment were still not fully kindled staying in stages 0 - III (p < 0.01). HFS group needed a higher number of stimulations to achieve stage III (p < 0.05) with respect to control group. How- ever, no significant differences in the cumulative daily afterdischarge duration were observed. HFS did not present sig- nificant differences compared with LFS in any of studied parameters. The findings suggest that unilateral HFS applied on hippocampus effectively inhibited the epileptogenic process induced by hippocampal rapid kindling. According to the comparative results about hippocampal rapid kindled animals stimulated with HFS and LFS (5 Hz), we found no conclusive information on which treatment is most efficient.Fil: Gori, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; ArgentinaFil: Pereyra, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; ArgentinaFil: Toibaro, Lucas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; ArgentinaFil: Brescacin, Carola. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; ArgentinaFil: Battaglia, Gerardo Ángel. Universidad Tecnológica Nacional; ArgentinaFil: Pastorino, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; ArgentinaFil: Smigliani, Ariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; ArgentinaFil: Galardi, Milagros. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; ArgentinaFil: Kochen, Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; ArgentinaAcademic Journals2013-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/1257Gori, María Belén; Pereyra, Magdalena; Toibaro, Lucas; Brescacin, Carola; Battaglia, Gerardo Ángel; et al.; Hippocampal high-frequency stimulation inhibites the progression of rapid kindling-induced seizure in rats; Academic Journals; Scientific Research And Essays; 2013; 4; 6-2013; 71-761992-2248enginfo:eu-repo/semantics/altIdentifier/doi/10.4236/nm.2013.42011info:eu-repo/semantics/altIdentifier/url/http://www.scirp.org/journal/nminfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:06:50Zoai:ri.conicet.gov.ar:11336/1257instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:06:50.697CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Hippocampal high-frequency stimulation inhibites the progression of rapid kindling-induced seizure in rats
title Hippocampal high-frequency stimulation inhibites the progression of rapid kindling-induced seizure in rats
spellingShingle Hippocampal high-frequency stimulation inhibites the progression of rapid kindling-induced seizure in rats
Gori, María Belén
HIPPOCAMPAL RAPID KINDLING
EPILEPSY
ELECTRICAL STIMULATION
HIGH-FREQUENCY STIMULATION
LOW-FREQUENCY STIMULATION
title_short Hippocampal high-frequency stimulation inhibites the progression of rapid kindling-induced seizure in rats
title_full Hippocampal high-frequency stimulation inhibites the progression of rapid kindling-induced seizure in rats
title_fullStr Hippocampal high-frequency stimulation inhibites the progression of rapid kindling-induced seizure in rats
title_full_unstemmed Hippocampal high-frequency stimulation inhibites the progression of rapid kindling-induced seizure in rats
title_sort Hippocampal high-frequency stimulation inhibites the progression of rapid kindling-induced seizure in rats
dc.creator.none.fl_str_mv Gori, María Belén
Pereyra, Magdalena
Toibaro, Lucas
Brescacin, Carola
Battaglia, Gerardo Ángel
Pastorino, Julieta
Smigliani, Ariela
Galardi, Milagros
Kochen, Silvia
author Gori, María Belén
author_facet Gori, María Belén
Pereyra, Magdalena
Toibaro, Lucas
Brescacin, Carola
Battaglia, Gerardo Ángel
Pastorino, Julieta
Smigliani, Ariela
Galardi, Milagros
Kochen, Silvia
author_role author
author2 Pereyra, Magdalena
Toibaro, Lucas
Brescacin, Carola
Battaglia, Gerardo Ángel
Pastorino, Julieta
Smigliani, Ariela
Galardi, Milagros
Kochen, Silvia
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv HIPPOCAMPAL RAPID KINDLING
EPILEPSY
ELECTRICAL STIMULATION
HIGH-FREQUENCY STIMULATION
LOW-FREQUENCY STIMULATION
topic HIPPOCAMPAL RAPID KINDLING
EPILEPSY
ELECTRICAL STIMULATION
HIGH-FREQUENCY STIMULATION
LOW-FREQUENCY STIMULATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.5
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Epilepsy is one of the most common serious neurological disorders. Pharmacoresistant epilepsy patients are poorly con-trolled or their seizures are refractory to drug treatment. Resective surgery is frequently a promising therapy in this population, however, not all the patients meet the eligibility criteria for the surgical treatment. Deep brain stimulation has been investigated in clinical studies and animal studies as an alternative treatment, but the optimal stimulation pa- rameters remain an issue. The present study was designed to investigate the effect of unilateral high-frequency stimula- tion (HFS) of hippocampus on seizure development by using the hippocampal rapid kindling method (hRK) in rats, and compared the results with those of low-frequency stimulation previously published by our group. We used male Wistar rats implanted with electrodes in the ventral hippocampus. All rats underwent hRK (biphasic square wave pulses, 20 Hz for 10 seconds) during three consecutive days (twelve stimulations per day). The control group (hRK; n = 7) received only RK stimulus, while the treated group (HFS-hRK; n = 9) received also HFS (biphasic square wave pulses, 130 Hz for 30 seconds) immediately before the RK stimulus, during three consecutive days. At the end of behavioral testing 78% (p < 0.01) of the animals receiving HFS treatment were still not fully kindled staying in stages 0 - III (p < 0.01). HFS group needed a higher number of stimulations to achieve stage III (p < 0.05) with respect to control group. How- ever, no significant differences in the cumulative daily afterdischarge duration were observed. HFS did not present sig- nificant differences compared with LFS in any of studied parameters. The findings suggest that unilateral HFS applied on hippocampus effectively inhibited the epileptogenic process induced by hippocampal rapid kindling. According to the comparative results about hippocampal rapid kindled animals stimulated with HFS and LFS (5 Hz), we found no conclusive information on which treatment is most efficient.
Fil: Gori, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: Pereyra, Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: Toibaro, Lucas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: Brescacin, Carola. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: Battaglia, Gerardo Ángel. Universidad Tecnológica Nacional; Argentina
Fil: Pastorino, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: Smigliani, Ariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: Galardi, Milagros. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: Kochen, Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
description Epilepsy is one of the most common serious neurological disorders. Pharmacoresistant epilepsy patients are poorly con-trolled or their seizures are refractory to drug treatment. Resective surgery is frequently a promising therapy in this population, however, not all the patients meet the eligibility criteria for the surgical treatment. Deep brain stimulation has been investigated in clinical studies and animal studies as an alternative treatment, but the optimal stimulation pa- rameters remain an issue. The present study was designed to investigate the effect of unilateral high-frequency stimula- tion (HFS) of hippocampus on seizure development by using the hippocampal rapid kindling method (hRK) in rats, and compared the results with those of low-frequency stimulation previously published by our group. We used male Wistar rats implanted with electrodes in the ventral hippocampus. All rats underwent hRK (biphasic square wave pulses, 20 Hz for 10 seconds) during three consecutive days (twelve stimulations per day). The control group (hRK; n = 7) received only RK stimulus, while the treated group (HFS-hRK; n = 9) received also HFS (biphasic square wave pulses, 130 Hz for 30 seconds) immediately before the RK stimulus, during three consecutive days. At the end of behavioral testing 78% (p < 0.01) of the animals receiving HFS treatment were still not fully kindled staying in stages 0 - III (p < 0.01). HFS group needed a higher number of stimulations to achieve stage III (p < 0.05) with respect to control group. How- ever, no significant differences in the cumulative daily afterdischarge duration were observed. HFS did not present sig- nificant differences compared with LFS in any of studied parameters. The findings suggest that unilateral HFS applied on hippocampus effectively inhibited the epileptogenic process induced by hippocampal rapid kindling. According to the comparative results about hippocampal rapid kindled animals stimulated with HFS and LFS (5 Hz), we found no conclusive information on which treatment is most efficient.
publishDate 2013
dc.date.none.fl_str_mv 2013-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/1257
Gori, María Belén; Pereyra, Magdalena; Toibaro, Lucas; Brescacin, Carola; Battaglia, Gerardo Ángel; et al.; Hippocampal high-frequency stimulation inhibites the progression of rapid kindling-induced seizure in rats; Academic Journals; Scientific Research And Essays; 2013; 4; 6-2013; 71-76
1992-2248
url http://hdl.handle.net/11336/1257
identifier_str_mv Gori, María Belén; Pereyra, Magdalena; Toibaro, Lucas; Brescacin, Carola; Battaglia, Gerardo Ángel; et al.; Hippocampal high-frequency stimulation inhibites the progression of rapid kindling-induced seizure in rats; Academic Journals; Scientific Research And Essays; 2013; 4; 6-2013; 71-76
1992-2248
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.4236/nm.2013.42011
info:eu-repo/semantics/altIdentifier/url/http://www.scirp.org/journal/nm
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Journals
publisher.none.fl_str_mv Academic Journals
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269976955715584
score 13.13397

Publicaciones similares