Immunodetection of heparin-binding growth associated molecule (pleiotrophin) in striatal interneurons

Autores
Taravini, Irene Rita Eloisa; Ferrario, Juan Esteban; Delbe, Jean; Ginestet, Laure; Debeir, Thomas; Courty, José; Murer, Mario Gustavo; Gershanik, Oscar Samuel; Raisman Vozari, Rita
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Pleiotrophin (PTN), a developmentally-regulated trophic factor, is over-expressed in the striatum of parkinsonian rats. Because striatal PTN can provide trophic support to dopamine neurons, we identified the cellular types containing PTN in the striatum of adult rats. By means of fluorescent double-immunolabeling, we found PTN to co-localize with a neuronal nuclei marker but not with glial fibrillary acidic protein. The number, distribution, and morphology of the PTN-immunolabeled cells suggested that they were interneurons. Further double-immunolabeling studies ruled out PTN localization to calretinin- and parvalbumin-containing interneurons. Instead, ∼40% of the PTN-immunolabeled neurons contained nitric oxide synthase or somatostatin and ∼60% expressed the vesicular acetylcholine transporter, supporting that they were GABAergic nitric oxide synthase/somatostatin-containing and cholinergic interneurons. Further work is necessary to determine if PTN from striatal interneurons can provide trophic support to dopamine neurons.
Fil: Taravini, Irene Rita Eloisa.
Fil: Ferrario, Juan Esteban.
Fil: Jean Delbe.
Fil: Laure Ginestet.
Fil: Thomas Debeir.
Fil: José Courty.
Fil: M. Gustavo Murer. FACULTAD DE MEDICINA; Argentina
Fil: Gershanik, Oscar Samuel. ININFA; Argentina
Fil: Rita Raisman-Vozari.
Materia
PLEIOTROPHIN
STRIATAL INTERNEURON
IMMUNOHISTOCHEMISTRY
PARKINSON´S DISEASE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/94392
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/94392
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Immunodetection of heparin-binding growth associated molecule (pleiotrophin) in striatal interneuronsTaravini, Irene Rita EloisaFerrario, Juan EstebanDelbe, JeanGinestet, LaureDebeir, ThomasCourty, JoséMurer, Mario GustavoGershanik, Oscar SamuelRaisman Vozari, RitaPLEIOTROPHINSTRIATAL INTERNEURONIMMUNOHISTOCHEMISTRYPARKINSON´S DISEASEhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Pleiotrophin (PTN), a developmentally-regulated trophic factor, is over-expressed in the striatum of parkinsonian rats. Because striatal PTN can provide trophic support to dopamine neurons, we identified the cellular types containing PTN in the striatum of adult rats. By means of fluorescent double-immunolabeling, we found PTN to co-localize with a neuronal nuclei marker but not with glial fibrillary acidic protein. The number, distribution, and morphology of the PTN-immunolabeled cells suggested that they were interneurons. Further double-immunolabeling studies ruled out PTN localization to calretinin- and parvalbumin-containing interneurons. Instead, ∼40% of the PTN-immunolabeled neurons contained nitric oxide synthase or somatostatin and ∼60% expressed the vesicular acetylcholine transporter, supporting that they were GABAergic nitric oxide synthase/somatostatin-containing and cholinergic interneurons. Further work is necessary to determine if PTN from striatal interneurons can provide trophic support to dopamine neurons.Fil: Taravini, Irene Rita Eloisa.Fil: Ferrario, Juan Esteban.Fil: Jean Delbe.Fil: Laure Ginestet.Fil: Thomas Debeir.Fil: José Courty.Fil: M. Gustavo Murer. FACULTAD DE MEDICINA; ArgentinaFil: Gershanik, Oscar Samuel. ININFA; ArgentinaFil: Rita Raisman-Vozari.Elsevier Science2005-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/94392Taravini, Irene Rita Eloisa; Ferrario, Juan Esteban; Delbe, Jean; Ginestet, Laure; Debeir, Thomas; et al.; Immunodetection of heparin-binding growth associated molecule (pleiotrophin) in striatal interneurons; Elsevier Science; Brain Research; 1066; 1-2; 12-2005; 196-2000006-8993CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0006899305014460info:eu-repo/semantics/altIdentifier/doi/10.1016/j.brainres.2005.10.055info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:56:35Zoai:ri.conicet.gov.ar:11336/94392instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:56:36.132CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Immunodetection of heparin-binding growth associated molecule (pleiotrophin) in striatal interneurons
title Immunodetection of heparin-binding growth associated molecule (pleiotrophin) in striatal interneurons
spellingShingle Immunodetection of heparin-binding growth associated molecule (pleiotrophin) in striatal interneurons
Taravini, Irene Rita Eloisa
PLEIOTROPHIN
STRIATAL INTERNEURON
IMMUNOHISTOCHEMISTRY
PARKINSON´S DISEASE
title_short Immunodetection of heparin-binding growth associated molecule (pleiotrophin) in striatal interneurons
title_full Immunodetection of heparin-binding growth associated molecule (pleiotrophin) in striatal interneurons
title_fullStr Immunodetection of heparin-binding growth associated molecule (pleiotrophin) in striatal interneurons
title_full_unstemmed Immunodetection of heparin-binding growth associated molecule (pleiotrophin) in striatal interneurons
title_sort Immunodetection of heparin-binding growth associated molecule (pleiotrophin) in striatal interneurons
dc.creator.none.fl_str_mv Taravini, Irene Rita Eloisa
Ferrario, Juan Esteban
Delbe, Jean
Ginestet, Laure
Debeir, Thomas
Courty, José
Murer, Mario Gustavo
Gershanik, Oscar Samuel
Raisman Vozari, Rita
author Taravini, Irene Rita Eloisa
author_facet Taravini, Irene Rita Eloisa
Ferrario, Juan Esteban
Delbe, Jean
Ginestet, Laure
Debeir, Thomas
Courty, José
Murer, Mario Gustavo
Gershanik, Oscar Samuel
Raisman Vozari, Rita
author_role author
author2 Ferrario, Juan Esteban
Delbe, Jean
Ginestet, Laure
Debeir, Thomas
Courty, José
Murer, Mario Gustavo
Gershanik, Oscar Samuel
Raisman Vozari, Rita
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv PLEIOTROPHIN
STRIATAL INTERNEURON
IMMUNOHISTOCHEMISTRY
PARKINSON´S DISEASE
topic PLEIOTROPHIN
STRIATAL INTERNEURON
IMMUNOHISTOCHEMISTRY
PARKINSON´S DISEASE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Pleiotrophin (PTN), a developmentally-regulated trophic factor, is over-expressed in the striatum of parkinsonian rats. Because striatal PTN can provide trophic support to dopamine neurons, we identified the cellular types containing PTN in the striatum of adult rats. By means of fluorescent double-immunolabeling, we found PTN to co-localize with a neuronal nuclei marker but not with glial fibrillary acidic protein. The number, distribution, and morphology of the PTN-immunolabeled cells suggested that they were interneurons. Further double-immunolabeling studies ruled out PTN localization to calretinin- and parvalbumin-containing interneurons. Instead, ∼40% of the PTN-immunolabeled neurons contained nitric oxide synthase or somatostatin and ∼60% expressed the vesicular acetylcholine transporter, supporting that they were GABAergic nitric oxide synthase/somatostatin-containing and cholinergic interneurons. Further work is necessary to determine if PTN from striatal interneurons can provide trophic support to dopamine neurons.
Fil: Taravini, Irene Rita Eloisa.
Fil: Ferrario, Juan Esteban.
Fil: Jean Delbe.
Fil: Laure Ginestet.
Fil: Thomas Debeir.
Fil: José Courty.
Fil: M. Gustavo Murer. FACULTAD DE MEDICINA; Argentina
Fil: Gershanik, Oscar Samuel. ININFA; Argentina
Fil: Rita Raisman-Vozari.
description Pleiotrophin (PTN), a developmentally-regulated trophic factor, is over-expressed in the striatum of parkinsonian rats. Because striatal PTN can provide trophic support to dopamine neurons, we identified the cellular types containing PTN in the striatum of adult rats. By means of fluorescent double-immunolabeling, we found PTN to co-localize with a neuronal nuclei marker but not with glial fibrillary acidic protein. The number, distribution, and morphology of the PTN-immunolabeled cells suggested that they were interneurons. Further double-immunolabeling studies ruled out PTN localization to calretinin- and parvalbumin-containing interneurons. Instead, ∼40% of the PTN-immunolabeled neurons contained nitric oxide synthase or somatostatin and ∼60% expressed the vesicular acetylcholine transporter, supporting that they were GABAergic nitric oxide synthase/somatostatin-containing and cholinergic interneurons. Further work is necessary to determine if PTN from striatal interneurons can provide trophic support to dopamine neurons.
publishDate 2005
dc.date.none.fl_str_mv 2005-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/94392
Taravini, Irene Rita Eloisa; Ferrario, Juan Esteban; Delbe, Jean; Ginestet, Laure; Debeir, Thomas; et al.; Immunodetection of heparin-binding growth associated molecule (pleiotrophin) in striatal interneurons; Elsevier Science; Brain Research; 1066; 1-2; 12-2005; 196-200
0006-8993
CONICET Digital
CONICET
url http://hdl.handle.net/11336/94392
identifier_str_mv Taravini, Irene Rita Eloisa; Ferrario, Juan Esteban; Delbe, Jean; Ginestet, Laure; Debeir, Thomas; et al.; Immunodetection of heparin-binding growth associated molecule (pleiotrophin) in striatal interneurons; Elsevier Science; Brain Research; 1066; 1-2; 12-2005; 196-200
0006-8993
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0006899305014460
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.brainres.2005.10.055
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269412358356992
score 13.13397

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