Anatomical, Histochemical and Cytogenetic Features of Doryopteris triphylla (Pteridaceae)

Autores
Neira, Diego Amando; Andrada, Aldo Rubén; Páez, Valeria de los Ángeles; Rodriguez, Ana María del Valle; Ríos, Norma Fabiana; Martinez, Olga Gladys; Hernández, Marcela Alicia
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Doryopteris triphylla (Pteridaceae-Cheilanthoideae) grows in xeric habitats inBrazil, Paraguay, Uruguay and Argentina. The aim of this study was to characterizeD. triphylla anatomically, histochemically and cytogenetically. Foranatomical characterization, rhizomes, roots, petioles and leaves were madeand then stained using Safranine-Astra Blue for further observations. Leafblades were also cleared. For histochemical analysis, leaf cross sections werestained with different reagents to identify glandular trichomes compounds.For cytogenetic characterization, a karyogram was performed using laboratorycultivated roots. Results show a dictyostelic rhizome covered with scales withapical secreting gland; diarch roots; petiole cross-sections show thick cuticle,uniseriate epidermis, parenchymatic cortex cells with thick walls and a vascularbundle with two xylem groups; and hypostomatic fronds with glandulartrichomes. Histochemical studies of secretion products of the glandular trichomeswere positive for polysaccharides, pectins, lipids, acid lipids, dihydroxyphenols,phenols and flavonoids. Cytogenetically, D. triphylla is describedas a diploid species (2n = 60), with chromosomes gradually decreasingin size. The apical glands in scales of rhizomes, the presence of two xylemgroups in the vascular bundle in the petiole and the glandular trichomes onthe abaxial surface are new contributions to the species. The type of chemicalproducts secreted by glandular leaf trichomes and karyotype estimation isshown for the first time in this species.
Fil: Neira, Diego Amando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación Miguel Lillo; Argentina
Fil: Andrada, Aldo Rubén. Fundación Miguel Lillo; Argentina
Fil: Páez, Valeria de los Ángeles. Fundación Miguel Lillo; Argentina
Fil: Rodriguez, Ana María del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Ríos, Norma Fabiana. Fundación Miguel Lillo; Argentina
Fil: Martinez, Olga Gladys. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Bio y Geociencias del NOA. Universidad Nacional de Salta. Facultad de Ciencias Naturales. Museo de Ciencias Naturales. Instituto de Bio y Geociencias del NOA; Argentina
Fil: Hernández, Marcela Alicia. Fundación Miguel Lillo; Argentina
Materia
ANATOMY
HISTOCHEMISTRY
KARYOGRAM
PTERIDACEAE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/45882
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/45882
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Anatomical, Histochemical and Cytogenetic Features of Doryopteris triphylla (Pteridaceae)Neira, Diego AmandoAndrada, Aldo RubénPáez, Valeria de los ÁngelesRodriguez, Ana María del ValleRíos, Norma FabianaMartinez, Olga GladysHernández, Marcela AliciaANATOMYHISTOCHEMISTRYKARYOGRAMPTERIDACEAEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Doryopteris triphylla (Pteridaceae-Cheilanthoideae) grows in xeric habitats inBrazil, Paraguay, Uruguay and Argentina. The aim of this study was to characterizeD. triphylla anatomically, histochemically and cytogenetically. Foranatomical characterization, rhizomes, roots, petioles and leaves were madeand then stained using Safranine-Astra Blue for further observations. Leafblades were also cleared. For histochemical analysis, leaf cross sections werestained with different reagents to identify glandular trichomes compounds.For cytogenetic characterization, a karyogram was performed using laboratorycultivated roots. Results show a dictyostelic rhizome covered with scales withapical secreting gland; diarch roots; petiole cross-sections show thick cuticle,uniseriate epidermis, parenchymatic cortex cells with thick walls and a vascularbundle with two xylem groups; and hypostomatic fronds with glandulartrichomes. Histochemical studies of secretion products of the glandular trichomeswere positive for polysaccharides, pectins, lipids, acid lipids, dihydroxyphenols,phenols and flavonoids. Cytogenetically, D. triphylla is describedas a diploid species (2n = 60), with chromosomes gradually decreasingin size. The apical glands in scales of rhizomes, the presence of two xylemgroups in the vascular bundle in the petiole and the glandular trichomes onthe abaxial surface are new contributions to the species. The type of chemicalproducts secreted by glandular leaf trichomes and karyotype estimation isshown for the first time in this species.Fil: Neira, Diego Amando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación Miguel Lillo; ArgentinaFil: Andrada, Aldo Rubén. Fundación Miguel Lillo; ArgentinaFil: Páez, Valeria de los Ángeles. Fundación Miguel Lillo; ArgentinaFil: Rodriguez, Ana María del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; ArgentinaFil: Ríos, Norma Fabiana. Fundación Miguel Lillo; ArgentinaFil: Martinez, Olga Gladys. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Bio y Geociencias del NOA. Universidad Nacional de Salta. Facultad de Ciencias Naturales. Museo de Ciencias Naturales. Instituto de Bio y Geociencias del NOA; ArgentinaFil: Hernández, Marcela Alicia. Fundación Miguel Lillo; ArgentinaScientific Research2017-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/45882Neira, Diego Amando; Andrada, Aldo Rubén; Páez, Valeria de los Ángeles; Rodriguez, Ana María del Valle; Ríos, Norma Fabiana; et al.; Anatomical, Histochemical and Cytogenetic Features of Doryopteris triphylla (Pteridaceae); Scientific Research; American Journal of Plant Sciences; 8; 4; 3-2017; 907-9202158-2742CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.scirp.org/journal/doi.aspx?DOI=10.4236/ajps.2017.84061info:eu-repo/semantics/altIdentifier/doi/10.4236/ajps.2017.84061info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:15:07Zoai:ri.conicet.gov.ar:11336/45882instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:15:07.277CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Anatomical, Histochemical and Cytogenetic Features of Doryopteris triphylla (Pteridaceae)
title Anatomical, Histochemical and Cytogenetic Features of Doryopteris triphylla (Pteridaceae)
spellingShingle Anatomical, Histochemical and Cytogenetic Features of Doryopteris triphylla (Pteridaceae)
Neira, Diego Amando
ANATOMY
HISTOCHEMISTRY
KARYOGRAM
PTERIDACEAE
title_short Anatomical, Histochemical and Cytogenetic Features of Doryopteris triphylla (Pteridaceae)
title_full Anatomical, Histochemical and Cytogenetic Features of Doryopteris triphylla (Pteridaceae)
title_fullStr Anatomical, Histochemical and Cytogenetic Features of Doryopteris triphylla (Pteridaceae)
title_full_unstemmed Anatomical, Histochemical and Cytogenetic Features of Doryopteris triphylla (Pteridaceae)
title_sort Anatomical, Histochemical and Cytogenetic Features of Doryopteris triphylla (Pteridaceae)
dc.creator.none.fl_str_mv Neira, Diego Amando
Andrada, Aldo Rubén
Páez, Valeria de los Ángeles
Rodriguez, Ana María del Valle
Ríos, Norma Fabiana
Martinez, Olga Gladys
Hernández, Marcela Alicia
author Neira, Diego Amando
author_facet Neira, Diego Amando
Andrada, Aldo Rubén
Páez, Valeria de los Ángeles
Rodriguez, Ana María del Valle
Ríos, Norma Fabiana
Martinez, Olga Gladys
Hernández, Marcela Alicia
author_role author
author2 Andrada, Aldo Rubén
Páez, Valeria de los Ángeles
Rodriguez, Ana María del Valle
Ríos, Norma Fabiana
Martinez, Olga Gladys
Hernández, Marcela Alicia
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ANATOMY
HISTOCHEMISTRY
KARYOGRAM
PTERIDACEAE
topic ANATOMY
HISTOCHEMISTRY
KARYOGRAM
PTERIDACEAE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Doryopteris triphylla (Pteridaceae-Cheilanthoideae) grows in xeric habitats inBrazil, Paraguay, Uruguay and Argentina. The aim of this study was to characterizeD. triphylla anatomically, histochemically and cytogenetically. Foranatomical characterization, rhizomes, roots, petioles and leaves were madeand then stained using Safranine-Astra Blue for further observations. Leafblades were also cleared. For histochemical analysis, leaf cross sections werestained with different reagents to identify glandular trichomes compounds.For cytogenetic characterization, a karyogram was performed using laboratorycultivated roots. Results show a dictyostelic rhizome covered with scales withapical secreting gland; diarch roots; petiole cross-sections show thick cuticle,uniseriate epidermis, parenchymatic cortex cells with thick walls and a vascularbundle with two xylem groups; and hypostomatic fronds with glandulartrichomes. Histochemical studies of secretion products of the glandular trichomeswere positive for polysaccharides, pectins, lipids, acid lipids, dihydroxyphenols,phenols and flavonoids. Cytogenetically, D. triphylla is describedas a diploid species (2n = 60), with chromosomes gradually decreasingin size. The apical glands in scales of rhizomes, the presence of two xylemgroups in the vascular bundle in the petiole and the glandular trichomes onthe abaxial surface are new contributions to the species. The type of chemicalproducts secreted by glandular leaf trichomes and karyotype estimation isshown for the first time in this species.
Fil: Neira, Diego Amando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación Miguel Lillo; Argentina
Fil: Andrada, Aldo Rubén. Fundación Miguel Lillo; Argentina
Fil: Páez, Valeria de los Ángeles. Fundación Miguel Lillo; Argentina
Fil: Rodriguez, Ana María del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Ríos, Norma Fabiana. Fundación Miguel Lillo; Argentina
Fil: Martinez, Olga Gladys. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Bio y Geociencias del NOA. Universidad Nacional de Salta. Facultad de Ciencias Naturales. Museo de Ciencias Naturales. Instituto de Bio y Geociencias del NOA; Argentina
Fil: Hernández, Marcela Alicia. Fundación Miguel Lillo; Argentina
description Doryopteris triphylla (Pteridaceae-Cheilanthoideae) grows in xeric habitats inBrazil, Paraguay, Uruguay and Argentina. The aim of this study was to characterizeD. triphylla anatomically, histochemically and cytogenetically. Foranatomical characterization, rhizomes, roots, petioles and leaves were madeand then stained using Safranine-Astra Blue for further observations. Leafblades were also cleared. For histochemical analysis, leaf cross sections werestained with different reagents to identify glandular trichomes compounds.For cytogenetic characterization, a karyogram was performed using laboratorycultivated roots. Results show a dictyostelic rhizome covered with scales withapical secreting gland; diarch roots; petiole cross-sections show thick cuticle,uniseriate epidermis, parenchymatic cortex cells with thick walls and a vascularbundle with two xylem groups; and hypostomatic fronds with glandulartrichomes. Histochemical studies of secretion products of the glandular trichomeswere positive for polysaccharides, pectins, lipids, acid lipids, dihydroxyphenols,phenols and flavonoids. Cytogenetically, D. triphylla is describedas a diploid species (2n = 60), with chromosomes gradually decreasingin size. The apical glands in scales of rhizomes, the presence of two xylemgroups in the vascular bundle in the petiole and the glandular trichomes onthe abaxial surface are new contributions to the species. The type of chemicalproducts secreted by glandular leaf trichomes and karyotype estimation isshown for the first time in this species.
publishDate 2017
dc.date.none.fl_str_mv 2017-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/45882
Neira, Diego Amando; Andrada, Aldo Rubén; Páez, Valeria de los Ángeles; Rodriguez, Ana María del Valle; Ríos, Norma Fabiana; et al.; Anatomical, Histochemical and Cytogenetic Features of Doryopteris triphylla (Pteridaceae); Scientific Research; American Journal of Plant Sciences; 8; 4; 3-2017; 907-920
2158-2742
CONICET Digital
CONICET
url http://hdl.handle.net/11336/45882
identifier_str_mv Neira, Diego Amando; Andrada, Aldo Rubén; Páez, Valeria de los Ángeles; Rodriguez, Ana María del Valle; Ríos, Norma Fabiana; et al.; Anatomical, Histochemical and Cytogenetic Features of Doryopteris triphylla (Pteridaceae); Scientific Research; American Journal of Plant Sciences; 8; 4; 3-2017; 907-920
2158-2742
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.scirp.org/journal/doi.aspx?DOI=10.4236/ajps.2017.84061
info:eu-repo/semantics/altIdentifier/doi/10.4236/ajps.2017.84061
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Scientific Research
publisher.none.fl_str_mv Scientific Research
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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